Investigating the deep supercooling ability of an Alaskan beetle, Cucujus clavipes puniceus, via high throughput proteomics
- PMID:22094879
- DOI: 10.1016/j.jprot.2011.10.034
Investigating the deep supercooling ability of an Alaskan beetle, Cucujus clavipes puniceus, via high throughput proteomics
Abstract
Cucujus clavipes puniceus is a freeze avoiding beetle capable of surviving the long, extremely cold winters of the Interior of Alaska. Previous studies showed that some individuals typically supercool to mean values of approximately -40 °C, with some individuals supercooling to as low as -58 °C, but these non-deep supercooling (NDSC) individuals eventually freeze if temperatures drop below this. However, other larvae, especially if exposed to very cold temperatures, supercool even further. These deep supercooling (DSC) individuals do not freeze even if cooled to -100 °C. In addition, the body water of the DSC larvae vitrifies (turns to a glass) at glass transition temperatures of -58 to -70 °C. This study examines the proteomes of DSC and NDSC larvae to assess proteins that may contribute to or inhibit the DSC trait. Using high throughput proteomics, we identified 138 proteins and 513 Gene Ontology categories in the DSC group and 104 proteins and 573 GO categories in the NDSC group. GO categories enriched in DSC include alcohol metabolic process, cellular component morphogenesis, monosaccharide metabolic process, regulation of biological quality, extracellular region, structural molecule activity, and antioxidant activity. Proteins unique to DSC include alpha casein precursor, alpha-actinin, vimentin, tropomyosin, beta-lactoglobulin, immunoglobulins, tubulin, cuticle proteins and endothelins.
Copyright © 2011 Elsevier B.V. All rights reserved.
Similar articles
- Elucidating the biochemical overwintering adaptations of Larval Cucujus clavipes puniceus, a nonmodel organism, via high throughput proteomics.Carrasco MA, Buechler SA, Arnold RJ, Sformo T, Barnes BM, Duman JG.Carrasco MA, et al.J Proteome Res. 2011 Oct 7;10(10):4634-46. doi: 10.1021/pr200518y. Epub 2011 Sep 19.J Proteome Res. 2011.PMID:21923194
- Deep supercooling, vitrification and limited survival to -100{degrees}C in the Alaskan beetle Cucujus clavipes puniceus (Coleoptera: Cucujidae) larvae.Sformo T, Walters K, Jeannet K, Wowk B, Fahy GM, Barnes BM, Duman JG.Sformo T, et al.J Exp Biol. 2010 Feb 1;213(3):502-9. doi: 10.1242/jeb.035758.J Exp Biol. 2010.PMID:20086136
- Probability of freezing in the freeze-avoiding beetle larvae Cucujus clavipes puniceus (Coleoptera: Cucujidae) from interior Alaska.Sformo T, McIntyre J, Walters KR Jr, Barnes BM, Duman J.Sformo T, et al.J Insect Physiol. 2011 Aug;57(8):1170-7. doi: 10.1016/j.jinsphys.2011.04.011. Epub 2011 Apr 28.J Insect Physiol. 2011.PMID:21550349
- Reptile freeze tolerance: metabolism and gene expression.Storey KB.Storey KB.Cryobiology. 2006 Feb;52(1):1-16. doi: 10.1016/j.cryobiol.2005.09.005.Cryobiology. 2006.PMID:16321368Review.
- Antifreeze and ice nucleator proteins in terrestrial arthropods.Duman JG.Duman JG.Annu Rev Physiol. 2001;63:327-57. doi: 10.1146/annurev.physiol.63.1.327.Annu Rev Physiol. 2001.PMID:11181959Review.
Cited by
- From Phenology and Habitat Preferences to Climate Change: Importance of Citizen Science in Studying Insect Ecology in the Continental Scale with American Red Flat Bark Beetle,Cucujus clavipes, as a Model Species.Jaskuła R, Kolanowska M, Michalski M, Schwerk A.Jaskuła R, et al.Insects. 2021 Apr 20;12(4):369. doi: 10.3390/insects12040369.Insects. 2021.PMID:33924259Free PMC article.
- TMT-Based Quantitative Proteomic Profiling of OverwinteringLissorhoptrus oryzophilus.Xinxin Z, Shuang Y, Xunming Z, Shang W, Juhong Z, Jinghui X.Xinxin Z, et al.Front Physiol. 2020 Jan 21;10:1623. doi: 10.3389/fphys.2019.01623. eCollection 2019.Front Physiol. 2020.PMID:32038298Free PMC article.
- Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture.Eskandari A, Leow TC, Rahman MBA, Oslan SN.Eskandari A, et al.Biomolecules. 2020 Dec 9;10(12):1649. doi: 10.3390/biom10121649.Biomolecules. 2020.PMID:33317024Free PMC article.Review.
- Autumn shifts in cold tolerance metabolites in overwintering adult mountain pine beetles.Thompson KM, Huber DPW, Murray BW.Thompson KM, et al.PLoS One. 2020 Jan 8;15(1):e0227203. doi: 10.1371/journal.pone.0227203. eCollection 2020.PLoS One. 2020.PMID:31914144Free PMC article.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources