Structural messenger RNA contains cytokeratin polymerization and depolymerization signals
- PMID:21987223
- DOI: 10.1007/s00441-011-1255-x
Structural messenger RNA contains cytokeratin polymerization and depolymerization signals
Abstract
We have previously shown that VegT mRNA plays a structural (translation-independent) role in the organization of the cytokeratin cytoskeleton in Xenopus oocytes. The depletion of VegT mRNA causes the fragmentation of the cytokeratin network in the vegetal cortex of Xenopus oocytes. This effect can be rescued by the injection of synthetic VegT RNA into the oocyte. Here, we show that the structural function of VegT mRNA in Xenopus oocyte depends on its combinatory signals for the induction or facilitation and for the maintenance of the depolymerization vs. polymerization status of cytokeratin filaments and that the 300-nucleotide fragment of VegT RNA isolated from the context of the entire molecule induces and maintains the depolymerization of cytokeratin filaments when injected into Xenopus oocytes. A computational analysis of three homologous Xenopus VegT mRNAs has revealed the presence, within this 300-nucleotide region, of a conserved base-pairing (hairpin) configuration that might function in RNA/protein interactions.
Similar articles
- Organization of cytokeratin cytoskeleton and germ plasm in the vegetal cortex of Xenopus laevis oocytes depends on coding and non-coding RNAs: three-dimensional and ultrastructural analysis.Kloc M, Bilinski S, Dougherty MT.Kloc M, et al.Exp Cell Res. 2007 May 1;313(8):1639-51. doi: 10.1016/j.yexcr.2007.02.018. Epub 2007 Feb 28.Exp Cell Res. 2007.PMID:17376434Free PMC article.
- Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.Kloc M, Wilk K, Vargas D, Shirato Y, Bilinski S, Etkin LD.Kloc M, et al.Development. 2005 Aug;132(15):3445-57. doi: 10.1242/dev.01919. Epub 2005 Jul 6.Development. 2005.PMID:16000384
- RNA of AmVegT, the axolotl orthologue of the Xenopus meso-endodermal determinant, is not localized in the oocyte.Nath K, Elinson RP.Nath K, et al.Gene Expr Patterns. 2007 Jan;7(1-2):197-201. doi: 10.1016/j.modgep.2006.07.003. Epub 2006 Jul 16.Gene Expr Patterns. 2007.PMID:16920404
- Binary function of mRNA.Kloc M, Foreman V, Reddy SA.Kloc M, et al.Biochimie. 2011 Nov;93(11):1955-61. doi: 10.1016/j.biochi.2011.07.008. Epub 2011 Jul 28.Biochimie. 2011.PMID:21784124Review.
- RNA localization in Xenopus oocytes.Rand K, Yisraeli J.Rand K, et al.Results Probl Cell Differ. 2001;34:157-73. doi: 10.1007/978-3-540-40025-7_10.Results Probl Cell Differ. 2001.PMID:11288674Review.No abstract available.
Cited by
- Structural dynamics control the MicroRNA maturation pathway.Dallaire P, Tan H, Szulwach K, Ma C, Jin P, Major F.Dallaire P, et al.Nucleic Acids Res. 2016 Nov 16;44(20):9956-9964. doi: 10.1093/nar/gkw793. Epub 2016 Sep 19.Nucleic Acids Res. 2016.PMID:27651454Free PMC article.
- RNA sequences required for the noncoding function of oskar RNA also mediate regulation of Oskar protein expression by Bicoid Stability Factor.Ryu YH, Macdonald PM.Ryu YH, et al.Dev Biol. 2015 Nov 15;407(2):211-23. doi: 10.1016/j.ydbio.2015.09.014. Epub 2015 Oct 9.Dev Biol. 2015.PMID:26433064Free PMC article.
- Cytotopic localization by long noncoding RNAs.Batista PJ, Chang HY.Batista PJ, et al.Curr Opin Cell Biol. 2013 Apr;25(2):195-9. doi: 10.1016/j.ceb.2012.12.001. Epub 2012 Dec 29.Curr Opin Cell Biol. 2013.PMID:23279909Free PMC article.Review.
- Maternal messages to live by: a personal historical perspective.King ML.King ML.Genesis. 2017 Jan;55(1-2):10.1002/dvg.23007. doi: 10.1002/dvg.23007.Genesis. 2017.PMID:28095642Free PMC article.Review.
- oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.Kanke M, Jambor H, Reich J, Marches B, Gstir R, Ryu YH, Ephrussi A, Macdonald PM.Kanke M, et al.RNA. 2015 Jun;21(6):1096-109. doi: 10.1261/rna.048298.114. Epub 2015 Apr 10.RNA. 2015.PMID:25862242Free PMC article.
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources