The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate
- PMID:2186030
The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate
Abstract
The clpA gene, which codes for the ATP-binding subunit of the ATP-dependent Clp protease of Escherichia coli, has been sequenced. The coding region contains a single open reading frame for a protein of 758 amino acids; within the amino acid sequence are two consensus sequences for ATP-binding sites. The sequence of ClpA does not resemble that of other previously described ATPases or Lon, the other sequenced ATP-dependent protease of E. coli, except in the ATP-binding site consensus region. The clpA gene is expressed as a monocistronic message. Primer extension experiments define a major start point of transcription at -183 relative to the start of translation. A rho-independent terminator is located 23 bases beyond the end of the coding region. The ClpA protein is degraded in vivo in a Clp-dependent fashion (t1/2 approximately 60 min). A fusion protein containing the first 40 amino acids of ClpA fused in frame to beta-galactosidase is degraded very rapidly in a clpA+ host (t1/2 approximately 3 min) but not in a clpA- host. This fusion protein is the first Clp-specific substrate described.
Similar articles
- Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli.Maurizi MR, Clark WP, Katayama Y, Rudikoff S, Pumphrey J, Bowers B, Gottesman S.Maurizi MR, et al.J Biol Chem. 1990 Jul 25;265(21):12536-45.J Biol Chem. 1990.PMID:2197275
- The two-component, ATP-dependent Clp protease of Escherichia coli. Purification, cloning, and mutational analysis of the ATP-binding component.Katayama Y, Gottesman S, Pumphrey J, Rudikoff S, Clark WP, Maurizi MR.Katayama Y, et al.J Biol Chem. 1988 Oct 15;263(29):15226-36.J Biol Chem. 1988.PMID:3049606
- Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La.Chin DT, Goff SA, Webster T, Smith T, Goldberg AL.Chin DT, et al.J Biol Chem. 1988 Aug 25;263(24):11718-28.J Biol Chem. 1988.PMID:3042779
- Protease Ti (Clp), a multi-component ATP-dependent protease in Escherichia coli.Chung CH, Seol JH, Kang MS.Chung CH, et al.Biol Chem. 1996 Sep;377(9):549-54.Biol Chem. 1996.PMID:9067252Review.
- [Structural and functional characteristics of ATP-dependent Lon-proteinase from Escherichia coli].Rotanova TV.Rotanova TV.Bioorg Khim. 1999 Dec;25(12):883-91.Bioorg Khim. 1999.PMID:10734549Review.Russian.
Cited by
- ATP-dependent proteinases in bacteria.Hlavácek O, Váchová L.Hlavácek O, et al.Folia Microbiol (Praha). 2002;47(3):203-12. doi: 10.1007/BF02817639.Folia Microbiol (Praha). 2002.PMID:12094726Review.
- Proteome-wide alterations in Escherichia coli translation rates upon anaerobiosis.Kramer G, Sprenger RR, Nessen MA, Roseboom W, Speijer D, de Jong L, de Mattos MJ, Back J, de Koster CG.Kramer G, et al.Mol Cell Proteomics. 2010 Nov;9(11):2508-16. doi: 10.1074/mcp.M110.001826. Epub 2010 Aug 16.Mol Cell Proteomics. 2010.PMID:20713451Free PMC article.
- The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat.Shrader TE, Tobias JW, Varshavsky A.Shrader TE, et al.J Bacteriol. 1993 Jul;175(14):4364-74. doi: 10.1128/jb.175.14.4364-4374.1993.J Bacteriol. 1993.PMID:8331068Free PMC article.
- Reduction in adaptor amounts establishes degradation hierarchy among protease substrates.Yeom J, Gao X, Groisman EA.Yeom J, et al.Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4483-E4492. doi: 10.1073/pnas.1722246115. Epub 2018 Apr 23.Proc Natl Acad Sci U S A. 2018.PMID:29686082Free PMC article.
- Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana.Rosano GL, Bruch EM, Ceccarelli EA.Rosano GL, et al.J Biol Chem. 2011 Aug 26;286(34):29671-80. doi: 10.1074/jbc.M110.211946. Epub 2011 Jul 7.J Biol Chem. 2011.PMID:21737456Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous