Review
doi: 10.1016/j.chroma.2010.12.068. Epub 2010 Dec 23.Thin-layer chromatography with biological detection in phytochemistry
Affiliations
- PMID:21236438
- DOI: 10.1016/j.chroma.2010.12.068
Item in Clipboard
Review
Thin-layer chromatography with biological detection in phytochemistry
A Marston. J Chromatogr A..
Display options
Format
Display options
Format
Abstract
Bioautography on thin-layer chromatographic (TLC) plates is a means of detecting the biological activity of a sample which has migrated on the plate with a suitable solvent. It only requires small amounts of sample and is ideal for the investigation of plant constituents, which often occur as complex mixtures. It can be used for the target-directed isolation of these constituents. In contrast to HPLC, many samples can be run at the same time on TLC. Organic solvents, which cause inactivation of enzymes or death of living organisms, can be completely removed before biological detection. Many bioassays are compatible with TLC. Antimicrobial, radical scavenging, antioxidant activities and enzyme inhibition feature among the tests that are employed.
Copyright © 2010 Elsevier B.V. All rights reserved.
Similar articles
- Bioautography detection in thin-layer chromatography.Choma IM, Grzelak EM.Choma IM, et al.J Chromatogr A. 2011 May 13;1218(19):2684-91. doi: 10.1016/j.chroma.2010.12.069. Epub 2010 Dec 23.J Chromatogr A. 2011.PMID:21232747Review.
- Development of chromatographic and free radical scavenging activity fingerprints by thin-layer chromatography for selected Salvia species.Cieśla Ł, Staszek D, Hajnos M, Kowalska T, Waksmundzka-Hajnos M.Cieśla Ł, et al.Phytochem Anal. 2011 Jan-Feb;22(1):59-65. doi: 10.1002/pca.1251. Epub 2010 Aug 26.Phytochem Anal. 2011.PMID:20799273
- Effect-directed analysis in food by thin-layer chromatography assays.Cabezudo I, Salazar MO, Ramallo IA, Furlan RLE.Cabezudo I, et al.Food Chem. 2022 Oct 1;390:132937. doi: 10.1016/j.foodchem.2022.132937. Epub 2022 Apr 9.Food Chem. 2022.PMID:35569399Review.
- Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts.Yang Z, Zhang X, Duan D, Song Z, Yang M, Li S.Yang Z, et al.J Sep Sci. 2009 Sep;32(18):3257-9. doi: 10.1002/jssc.200900266.J Sep Sci. 2009.PMID:19697313
- An introduction to planar chromatography and its application to natural products isolation.Gibbons S.Gibbons S.Methods Mol Biol. 2012;864:117-53. doi: 10.1007/978-1-61779-624-1_6.Methods Mol Biol. 2012.PMID:22367896
Cited by
- Thin-layer chromatographic (TLC) separations and bioassays of plant extracts to identify antimicrobial compounds.Kagan IA, Flythe MD.Kagan IA, et al.J Vis Exp. 2014 Mar 27;(85):51411. doi: 10.3791/51411.J Vis Exp. 2014.PMID:24747583Free PMC article.
- The Bioassay-Guided Fractionation and Identification of Potent Acetylcholinesterase Inhibitors fromNarcissus c.v. 'Hawera' Using Optimized Vacuum Liquid Chromatography, High Resolution Mass Spectrometry and Bioautography.Mroczek T, Dymek A, Widelski J, Wojtanowski KK.Mroczek T, et al.Metabolites. 2020 Oct 4;10(10):395. doi: 10.3390/metabo10100395.Metabolites. 2020.PMID:33020380Free PMC article.
- Xanthones from the roots of Moutabea guianensis Aubl.Filho Hda S, Pacheco LC, Andrade Eda S, Correa MJ, Araújo RN, Guilhon GM, Silva JK, Santos LS.Filho Hda S, et al.Molecules. 2014 Dec 23;20(1):127-34. doi: 10.3390/molecules20010127.Molecules. 2014.PMID:25546625Free PMC article.
- Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography.Jović MD, Agatonovic-Kustrin S, Ristivojević PM, Trifković JĐ, Morton DW.Jović MD, et al.Molecules. 2023 Oct 30;28(21):7346. doi: 10.3390/molecules28217346.Molecules. 2023.PMID:37959765Free PMC article.
- Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis.Sobstyl E, Szopa A, Dziurka M, Ekiert H, Nikolaichuk H, Choma IM.Sobstyl E, et al.Molecules. 2022 Mar 25;27(7):2116. doi: 10.3390/molecules27072116.Molecules. 2022.PMID:35408515Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources