Kinship and Y-chromosome analysis of 7th century human remains: novel DNA extraction and typing procedure for ancient material
- PMID:19480023
- PMCID: PMC2702742
- DOI: 10.3325/cmj.2009.50.286
Kinship and Y-chromosome analysis of 7th century human remains: novel DNA extraction and typing procedure for ancient material
Abstract
Aim: To develop novel DNA extraction and typing procedure for DNA identification of the 7th century human remains, determine the familiar relationship between the individuals, estimate the Y-chromosome haplogroup, and compare the Y-chromosome haplotype with the contemporary populations.
Methods: DNA from preserved femur samples was extracted using the modified silica-based extraction technique. Polymerase chain reaction amplification was performed using human identification kits MiniFiler, Identifiler, and Y-filer and also laboratory-developed and validated Y-chromosome short tandem repeat (STR) pentaplexes with short amplicons.
Results: For 244A, 244B, 244C samples, full autosomal DNA profiles (15 STR markers and Amelogenin) and for 244D, 244E, 244F samples, MiniFiler profiles were produced. Y-chromosome haplotypes consisting of up to 24 STR markers were determined and used to predict the Y-chromosome haplogroups and compare the resulting haplotypes with the current population. Samples 244A, 244B, 244C, and 244D belong to Y-chromosome haplogroup R1b and the samples 244E and 244F to haplogroup G2a. Comparison of ancient haplotypes with the current population yielded numerous close matches with genetic distance below 2.
Conclusion: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.
Figures
Similar articles
- Massively parallel sequencing of 17 commonly used forensic autosomal STRs and amelogenin with small amplicons.Kim EH, Lee HY, Yang IS, Jung SE, Yang WI, Shin KJ.Kim EH, et al.Forensic Sci Int Genet. 2016 May;22:1-7. doi: 10.1016/j.fsigen.2016.01.001. Epub 2016 Jan 8.Forensic Sci Int Genet. 2016.PMID:26799314
- Development and validation of a novel 29-plex Y-STR typing system for forensic application.Li M, Zhou W, Zhang Y, Huang L, Wang X, Wu J, Meng M, Wang H, Li C, Bian Y.Li M, et al.Forensic Sci Int Genet. 2020 Jan;44:102169. doi: 10.1016/j.fsigen.2019.102169. Epub 2019 Oct 1.Forensic Sci Int Genet. 2020.PMID:31614310
- Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits.Hill CR, Kline MC, Mulero JJ, Lagacé RE, Chang CW, Hennessy LK, Butler JM.Hill CR, et al.J Forensic Sci. 2007 Jul;52(4):870-3. doi: 10.1111/j.1556-4029.2007.00491.x. Epub 2007 Jun 6.J Forensic Sci. 2007.PMID:17553078
- Forensic typing of short tandem repeat markers on the X and Y chromosomes.Diegoli TM.Diegoli TM.Forensic Sci Int Genet. 2015 Sep;18:140-51. doi: 10.1016/j.fsigen.2015.03.013. Epub 2015 Mar 25.Forensic Sci Int Genet. 2015.PMID:25934544Review.
- Forensic use of Y-chromosome DNA: a general overview.Kayser M.Kayser M.Hum Genet. 2017 May;136(5):621-635. doi: 10.1007/s00439-017-1776-9. Epub 2017 Mar 17.Hum Genet. 2017.PMID:28315050Free PMC article.Review.
Cited by
- Results of a collaborative study on DNA identification of aged bone samples.Vanek D, Budowle B, Dubska-Votrubova J, Ambers A, Frolik J, Pospisek M, Al Afeefi AA, Al Hosani KI, Allen M, Al Naimi KS, Al Salafi D, Al Tayyari WAR, Arguetaa W, Bottinelli M, Bus MM, Cemper-Kiesslich J, Cepil O, De Cock G, Desmyter S, El Amri H, El Ossmani H, Galdies R, Grün S, Guidet F, Hoefges A, Iancu CB, Lotz P, Maresca A, Nagy M, Novotny J, Rachid H, Rothe J, Stenersen M, Stephenson M, Stevanovitch A, Strien J, Sumita DR, Vella J, Zander J.Vanek D, et al.Croat Med J. 2017 Jun 14;58(3):203-213. doi: 10.3325/cmj.2017.58.203.Croat Med J. 2017.PMID:28613037Free PMC article.
- Molecular genetic identification of skeletal remains from the Second World War Konfin I mass grave in Slovenia.Zupanic Pajnic I, Gornjak Pogorelc B, Balazic J.Zupanic Pajnic I, et al.Int J Legal Med. 2010 Jul;124(4):307-17. doi: 10.1007/s00414-010-0431-y. Epub 2010 Mar 10.Int J Legal Med. 2010.PMID:20217112Free PMC article.
- Effects of humic acid on DNA quantification with Quantifiler® Human DNA Quantification kit and short tandem repeat amplification efficiency.Seo SB, Lee HY, Zhang AH, Kim HY, Shin DH, Lee SD.Seo SB, et al.Int J Legal Med. 2012 Nov;126(6):961-8. doi: 10.1007/s00414-011-0616-z. Epub 2011 Sep 13.Int J Legal Med. 2012.PMID:21912926
- Highly efficient nuclear DNA typing of the World War II skeletal remains using three new autosomal short tandem repeat amplification kits with the extended European Standard Set of loci.Zupanic Pajnic I, Gornjak Pogorelc B, Balazic J, Zupanc T, Stefanic B.Zupanic Pajnic I, et al.Croat Med J. 2012 Feb 15;53(1):17-23. doi: 10.3325/cmj.2012.53.17.Croat Med J. 2012.PMID:22351574Free PMC article.
- Ancient DNA reveals male diffusion through the Neolithic Mediterranean route.Lacan M, Keyser C, Ricaut FX, Brucato N, Duranthon F, Guilaine J, Crubézy E, Ludes B.Lacan M, et al.Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9788-91. doi: 10.1073/pnas.1100723108. Epub 2011 May 31.Proc Natl Acad Sci U S A. 2011.PMID:21628562Free PMC article.
References
- Lassen C, Hummel S, Herrmann B. Molecular sex identification of stillborn and neonate individuals (“Traufkinder”) from the burial site Aegerten. Anthropol Anz. 2000;58:1–8. - PubMed
- Hummel S, Bramanti B, Finke T, Herrmann B. Evaluation of morphological sex determinations by molecular analyses. Anthropol Anz. 2000;58:9–13. - PubMed
- Schultes T, Hummel S, Herrmann B. Classification of isolated skeletal elements using aDNA typing. Anthropol Anz. 1997;55:207–16. [in German] - PubMed
- Alt K, Vach W. Kinship studies in skeletal remains – concepts and examples. In: Alt KW, Rösing FW, Teschler-Nicola M, editors. Dental anthropology. Fundamentals, limits and prospects. Wien: Springer; 1998.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources