Effects of bone-marrow mesenchymal stem cells transplanted into vitreous cavity of rat injured by ischemia/reperfusion
- PMID:19084985
- DOI: 10.1007/s00417-008-1009-y
Effects of bone-marrow mesenchymal stem cells transplanted into vitreous cavity of rat injured by ischemia/reperfusion
Abstract
Objective: To examine the survival, migration, integration, differentiation and the expression of various neurotrophic factors of bone-marrow mesenchymal stem cells (BMSCs) transplanted into the vitreous cavity of rats injured by ischemia/reperfusion(I/R).
Methods: The BMSCs were separated from rat marrow using the wall-sticking method, and cultured in vitro to expand. Flow cytometry detected the surface antigens of BMSCs. Ninety-six rats were randomly divided into four groups: normal control injected PBS(C+P), normal control injected BMSCs (C+B), ischemic/reperfusion injected PBS(I/R+P)and ischemic/reperfusion injected BMSCs(I/R+B). After retinal I/R injury was induced in each group by increasing intraocular pressure, 10 microl PBS and BMSC suspensions labeled by red fluorescence CM-Dil were immediately injected into the vitreous cavity. We observed the survival, migration and integration of BMSCs using confocal microscopy. The differentiation and expression of basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) of CM-Dil-labeled BMSCs were detected by immunofluorescent labeling and reserved by confocal microscopy. The expression of mRNA and proteins of bFGF, BDNF and CNTF were assayed by RT-PCR and Western Blot respectively.
Results: After transplantation to normal eyes, BMSCs labeled by CM-Dil were mostly present in the vitreous cavity, and did not migrate. After transplantation to I/R eyes, BMSCs labeled by CM-Dil were mostly present along with the inner limiting membrane. Only a few cells were integrated into the ganglion cell layer. Two or 4 weeks after transplantation, a few BMSCs labeled by CM-Dil were observed to express markers of neuron- neurone specific enolase (NSE), neurofilament (NF) and various neurotrophic factors. The BMSC-injected I/R model eyes showed less reduction in the number of RGCs than that of the I/R eyes with PBS injection.
Conclusions: BMSC transplantation is a valuable neuroprotection tool for the treatment of retina and optic nerve diseases.
Similar articles
- Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats.Zhu Q, Liu Z, Wang C, Nie L, He Y, Zhang Y, Liu X, Su G.Zhu Q, et al.Mol Med Rep. 2015 Oct;12(4):5691-700. doi: 10.3892/mmr.2015.4132. Epub 2015 Jul 28.Mol Med Rep. 2015.PMID:26238991Free PMC article.
- [Effects and mechanism of interleukin-17-modified mouse bone marrow mesenchymal stem cells on rejection reaction of allogeneic skin transplantation in mice].Ma TX, Xu YW, Jiang DY.Ma TX, et al.Zhonghua Shao Shang Za Zhi. 2020 Mar 20;36(3):234-243. doi: 10.3760/cma.j.cn501120-20190510-00232.Zhonghua Shao Shang Za Zhi. 2020.PMID:32241050Chinese.
- Transplantation of induced pluripotent stem cells without C-Myc attenuates retinal ischemia and reperfusion injury in rats.Fang IM, Yang CM, Yang CH, Chiou SH, Chen MS.Fang IM, et al.Exp Eye Res. 2013 Aug;113:49-59. doi: 10.1016/j.exer.2013.05.007. Epub 2013 May 28.Exp Eye Res. 2013.PMID:23726881
- Mesenchymal Stem Cell Migration and Tissue Repair.Fu X, Liu G, Halim A, Ju Y, Luo Q, Song AG.Fu X, et al.Cells. 2019 Jul 28;8(8):784. doi: 10.3390/cells8080784.Cells. 2019.PMID:31357692Free PMC article.Review.
- Functional Heterogeneity of Bone Marrow Mesenchymal Stem Cell Subpopulations in Physiology and Pathology.Ning K, Yang B, Chen M, Man G, Liu S, Wang DE, Xu H.Ning K, et al.Int J Mol Sci. 2022 Oct 7;23(19):11928. doi: 10.3390/ijms231911928.Int J Mol Sci. 2022.PMID:36233230Free PMC article.Review.
Cited by
- Stem Cell Ophthalmology Treatment Study (SCOTS) for retinal and optic nerve diseases: a preliminary report.Weiss JN, Levy S, Malkin A.Weiss JN, et al.Neural Regen Res. 2015 Jun;10(6):982-8. doi: 10.4103/1673-5374.158365.Neural Regen Res. 2015.PMID:26199618Free PMC article.
- Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats.Zhu Q, Liu Z, Wang C, Nie L, He Y, Zhang Y, Liu X, Su G.Zhu Q, et al.Mol Med Rep. 2015 Oct;12(4):5691-700. doi: 10.3892/mmr.2015.4132. Epub 2015 Jul 28.Mol Med Rep. 2015.PMID:26238991Free PMC article.
- Extracellular Vesicles as a Potential Therapy for Neonatal Conditions: State of the Art and Challenges in Clinical Translation.Matei AC, Antounians L, Zani A.Matei AC, et al.Pharmaceutics. 2019 Aug 11;11(8):404. doi: 10.3390/pharmaceutics11080404.Pharmaceutics. 2019.PMID:31405234Free PMC article.Review.
- Identification of barriers to retinal engraftment of transplanted stem cells.Johnson TV, Bull ND, Martin KR.Johnson TV, et al.Invest Ophthalmol Vis Sci. 2010 Feb;51(2):960-70. doi: 10.1167/iovs.09-3884. Epub 2009 Oct 22.Invest Ophthalmol Vis Sci. 2010.PMID:19850833Free PMC article.
- Cell-Based Neuroprotection of Retinal Ganglion Cells in Animal Models of Optic Neuropathies.Hu Y, Grodzki LM, Bartsch S, Bartsch U.Hu Y, et al.Biology (Basel). 2021 Nov 15;10(11):1181. doi: 10.3390/biology10111181.Biology (Basel). 2021.PMID:34827174Free PMC article.Review.
References
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources