Stem cell transcriptome profiling via massive-scale mRNA sequencing
- PMID:18516046
- DOI: 10.1038/nmeth.1223
Stem cell transcriptome profiling via massive-scale mRNA sequencing
Abstract
We developed a massive-scale RNA sequencing protocol, short quantitative random RNA libraries or SQRL, to survey the complexity, dynamics and sequence content of transcriptomes in a near-complete fashion. This method generates directional, random-primed, linear cDNA libraries that are optimized for next-generation short-tag sequencing. We surveyed the poly(A)(+) transcriptomes of undifferentiated mouse embryonic stem cells (ESCs) and embryoid bodies (EBs) at an unprecedented depth (10 Gb), using the Applied Biosystems SOLiD technology. These libraries capture the genomic landscape of expression, state-specific expression, single-nucleotide polymorphisms (SNPs), the transcriptional activity of repeat elements, and both known and new alternative splicing events. We investigated the impact of transcriptional complexity on current models of key signaling pathways controlling ESC pluripotency and differentiation, highlighting how SQRL can be used to characterize transcriptome content and dynamics in a quantitative and reproducible manner, and suggesting that our understanding of transcriptional complexity is far from complete.
Comment in
- The beginning of the end for microarrays?Shendure J.Shendure J.Nat Methods. 2008 Jul;5(7):585-7. doi: 10.1038/nmeth0708-585.Nat Methods. 2008.PMID:18587314No abstract available.
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