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.2008 Jun;117(1):37-48.
doi: 10.1007/s00122-008-0750-2. Epub 2008 Apr 1.

Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L

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Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L

L Gong et al. Theor Appl Genet.2008 Jun.

Abstract

Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer Olkürbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety "Lady Godiva" (O5) and the Italian crookneck variety "Bianco Friulano" (CN), which are the parents of our previous F(2) mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%.

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Fig. 1
Fig. 1
A genetic linkage map ofC. pepo showing only SSR, SCAR markers and the two morphological traits. Linkage groups (LGp) are numbered consecutively, subgroups are indicated bya andb. SCAR markers arebold, and morphological traits arebold and italic. Cumulative marker distances are given in cM on the left side of the linkage groups
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