Induction of pluripotent stem cells from adult human fibroblasts by defined factors
- PMID:18035408
- DOI: 10.1016/j.cell.2007.11.019
Induction of pluripotent stem cells from adult human fibroblasts by defined factors
Abstract
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
Comment in
- Induction of pluripotency: from mouse to human.Zaehres H, Schöler HR.Zaehres H, et al.Cell. 2007 Nov 30;131(5):834-5. doi: 10.1016/j.cell.2007.11.020.Cell. 2007.PMID:18045526
- Induced pluripotent cells mimicking human embryonic stem cells.Mohamadnejad M, Swenson ES.Mohamadnejad M, et al.Arch Iran Med. 2008 Jan;11(1):125-8.Arch Iran Med. 2008.PMID:18154436No abstract available.
- The undoing of differentiation by four defined factors: A big step forward towards generating patient specific pluripotent stem cells.Verfaillie C.Verfaillie C.J Hepatol. 2008 Nov;49(5):876-8. doi: 10.1016/j.jhep.2008.08.007. Epub 2008 Aug 21.J Hepatol. 2008.PMID:18814929No abstract available.
- Achieving pluripotency.Baumann K.Baumann K.Nat Rev Mol Cell Biol. 2010 Oct;11(10):677. doi: 10.1038/nrm2980.Nat Rev Mol Cell Biol. 2010.PMID:20861870No abstract available.
- Literature Watch: implications for transplantation.Muller YD, Meier RP, Bromberg JS, Bühler LH.Muller YD, et al.Am J Transplant. 2013 Jun;13(6):1377. doi: 10.1111/ajt.12322.Am J Transplant. 2013.PMID:23721549No abstract available.
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