Identification, cloning and sequencing of two major venom proteins from the box jellyfish, Chironex fleckeri
- PMID:17688901
- DOI: 10.1016/j.toxicon.2007.06.016
Identification, cloning and sequencing of two major venom proteins from the box jellyfish, Chironex fleckeri
Abstract
Two of the most abundant proteins found in the nematocysts of the box jellyfish Chironex fleckeri have been identified as C. fleckeri toxin-1 (CfTX-1) and toxin-2 (CfTX-2). The molecular masses of CfTX-1 and CfTX-2, as determined by SDS-PAGE, are approximately 43 and 45 kDa, respectively, and both proteins are strongly antigenic to commercially available box jellyfish antivenom and rabbit polyclonal antibodies raised against C. fleckeri nematocyst extracts. The amino acid sequences of mature CfTX-1 and CfTX-2 (436 and 445 residues, respectively) share significant homology with three known proteins: CqTX-A from Chiropsalmus quadrigatus, CrTXs from Carybdea rastoni and CaTX-A from Carybdea alata, all of which are lethal, haemolytic box jellyfish toxins. Multiple sequence alignment of the five jellyfish proteins has identified several short, but highly conserved regions of amino acids that coincide with a predicted transmembrane spanning region, referred to as TSR1, which may be involved in a pore-forming mechanism of action. Furthermore, remote protein homology predictions for CfTX-2 and CaTX-A suggest weak structural similarities to pore-forming insecticidal delta-endotoxins Cry1Aa, Cry3Bb and Cry3A.
Similar articles
- Partial purification of cytolytic venom proteins from the box jellyfish, Chironex fleckeri.Brinkman D, Burnell J.Brinkman D, et al.Toxicon. 2008 Apr;51(5):853-63. doi: 10.1016/j.toxicon.2007.12.017. Epub 2007 Dec 23.Toxicon. 2008.PMID:18243272
- Chironex fleckeri (box jellyfish) venom proteins: expansion of a cnidarian toxin family that elicits variable cytolytic and cardiovascular effects.Brinkman DL, Konstantakopoulos N, McInerney BV, Mulvenna J, Seymour JE, Isbister GK, Hodgson WC.Brinkman DL, et al.J Biol Chem. 2014 Feb 21;289(8):4798-812. doi: 10.1074/jbc.M113.534149. Epub 2014 Jan 8.J Biol Chem. 2014.PMID:24403082Free PMC article.
- A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus.Nagai H, Takuwa-Kuroda K, Nakao M, Oshiro N, Iwanaga S, Nakajima T.Nagai H, et al.Biosci Biotechnol Biochem. 2002 Jan;66(1):97-102. doi: 10.1271/bbb.66.97.Biosci Biotechnol Biochem. 2002.PMID:11866126
- Biochemical and molecular characterisation of cubozoan protein toxins.Brinkman DL, Burnell JN.Brinkman DL, et al.Toxicon. 2009 Dec 15;54(8):1162-73. doi: 10.1016/j.toxicon.2009.02.006. Epub 2009 Feb 20.Toxicon. 2009.PMID:19232527Review.
- Australian venomous jellyfish, envenomation syndromes, toxins and therapy.Tibballs J.Tibballs J.Toxicon. 2006 Dec 1;48(7):830-59. doi: 10.1016/j.toxicon.2006.07.020. Epub 2006 Jul 15.Toxicon. 2006.PMID:16928389Review.
Cited by
- Evolution of the Cytolytic Pore-Forming Proteins (Actinoporins) in Sea Anemones.Macrander J, Daly M.Macrander J, et al.Toxins (Basel). 2016 Dec 8;8(12):368. doi: 10.3390/toxins8120368.Toxins (Basel). 2016.PMID:27941639Free PMC article.
- Structural Characterisation of Predicted Helical Regions in theChironex fleckeri CfTX-1 Toxin.Andreosso A, Bansal PS, Smout MJ, Wilson D, Seymour JE, Daly NL.Andreosso A, et al.Mar Drugs. 2018 Jun 7;16(6):201. doi: 10.3390/md16060201.Mar Drugs. 2018.PMID:29880743Free PMC article.
- Crude venom from nematocysts of Pelagia noctiluca (Cnidaria: Scyphozoa) elicits a sodium conductance in the plasma membrane of mammalian cells.Morabito R, Costa R, Rizzo V, Remigante A, Nofziger C, La Spada G, Marino A, Paulmichl M, Dossena S.Morabito R, et al.Sci Rep. 2017 Jan 23;7:41065. doi: 10.1038/srep41065.Sci Rep. 2017.PMID:28112211Free PMC article.
- Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms' Lytic Activity Using Mass Spectrometry and Zymography.Yue Y, Yu H, Li R, Xing R, Liu S, Li K, Wang X, Chen X, Li P.Yue Y, et al.Toxins (Basel). 2017 Jan 26;9(2):47. doi: 10.3390/toxins9020047.Toxins (Basel). 2017.PMID:28134758Free PMC article.
- Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea AnemoneStichodactyla helianthus.Rivera-de-Torre E, Palacios-Ortega J, Slotte JP, Gavilanes JG, Martínez-Del-Pozo Á, García-Linares S.Rivera-de-Torre E, et al.Int J Mol Sci. 2020 Nov 24;21(23):8915. doi: 10.3390/ijms21238915.Int J Mol Sci. 2020.PMID:33255441Free PMC article.Review.
Publication types
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources