doi: 10.1128/JB.01927-06. Epub 2007 Apr 6.
Molecular analysis of genes in Nostoc punctiforme involved in pilus biogenesis and plant infection
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- PMID:17416648
- PMCID: PMC1913353
- DOI: 10.1128/JB.01927-06
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Molecular analysis of genes in Nostoc punctiforme involved in pilus biogenesis and plant infection
Paula S Duggan et al. J Bacteriol.2007 Jun.
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Abstract
Hormogonia are the infective agents in many cyanobacterium-plant symbioses. Pilus-like appendages are expressed on the hormogonium surface, and mutations in pil-like genes altered surface piliation and reduced symbiotic competency. This is the first molecular evidence that pilus biogenesis in a filamentous cyanobacterium requires a type IV pilus system.
Figures
Conserved motifs of theN. punctiforme PilT proteins (NpR0117 and NpF2507 proteins),P. aeruginosa PilT (Pseu),Synechocystis strain PCC 6803 PilT1, andSynechocystis strain PCC 6803 PilT2. Black boxes indicate identical or conserved residues in all five of the sequences. Protein sequences were aligned using the program ClustalX. The Walker box motifs (thick lines), the Asp box and the His box (asterisks) are indicated. Note that only the central regions of the PilT sequences are shown.
Comparison of the cell surfaces of wild-type vegetative filaments and hormogonia. (A) Wild-type vegetative filaments; (B) wild-type hormogonia. Scale bars represent 1 μm. For electron microscopy, platinum wire (2 cm by 0.2 mm) was evaporated onto the surface of each sample by using an Edwards 306A high-vacuum coating unit and samples were viewed on a JEOL1200EX transmission electron microscope at 80 kV.
Presence of pilus-like appendages on the cell surface of hormogonia produced bypil mutants ofN. punctiforme. (A) Hormogonia of the NpR2800 mutant (pilD homologue); (B) hormogonia of the NpF2507 mutant (pilT homologue); (C) hormogonia of the NpR0117 mutant (pilT homologue). Scale bars represent 1 μm. At least 50 filaments were observed for each mutant phenotype, and the electron micrographs shown are representative of each strain.
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