A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling
- PMID:16728976
- DOI: 10.1038/nature04815
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling
Abstract
Lysine methylation of histones is recognized as an important component of an epigenetic indexing system demarcating transcriptionally active and inactive chromatin domains. Trimethylation of histone H3 lysine 4 (H3K4me3) marks transcription start sites of virtually all active genes. Recently, we reported that the WD40-repeat protein WDR5 is important for global levels of H3K4me3 and control of HOX gene expression. Here we show that a plant homeodomain (PHD) finger of nucleosome remodelling factor (NURF), an ISWI-containing ATP-dependent chromatin-remodelling complex, mediates a direct preferential association with H3K4me3 tails. Depletion of H3K4me3 causes partial release of the NURF subunit, BPTF (bromodomain and PHD finger transcription factor), from chromatin and defective recruitment of the associated ATPase, SNF2L (also known as ISWI and SMARCA1), to the HOXC8 promoter. Loss of BPTF in Xenopus embryos mimics WDR5 loss-of-function phenotypes, and compromises spatial control of Hox gene expression. These results strongly suggest that WDR5 and NURF function in a common biological pathway in vivo, and that NURF-mediated ATP-dependent chromatin remodelling is directly coupled to H3K4 trimethylation to maintain Hox gene expression patterns during development. We also identify a previously unknown function for the PHD finger as a highly specialized methyl-lysine-binding domain.
Comment in
- Gene regulation: a finger on the mark.Becker PB.Becker PB.Nature. 2006 Jul 6;442(7098):31-2. doi: 10.1038/442031a.Nature. 2006.PMID:16823438No abstract available.
Similar articles
- Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF.Li H, Ilin S, Wang W, Duncan EM, Wysocka J, Allis CD, Patel DJ.Li H, et al.Nature. 2006 Jul 6;442(7098):91-5. doi: 10.1038/nature04802. Epub 2006 May 21.Nature. 2006.PMID:16728978Free PMC article.
- Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger.Wang GG, Song J, Wang Z, Dormann HL, Casadio F, Li H, Luo JL, Patel DJ, Allis CD.Wang GG, et al.Nature. 2009 Jun 11;459(7248):847-51. doi: 10.1038/nature08036.Nature. 2009.PMID:19430464Free PMC article.
- Alternative splicing of NURF301 generates distinct NURF chromatin remodeling complexes with altered modified histone binding specificities.Kwon SY, Xiao H, Wu C, Badenhorst P.Kwon SY, et al.PLoS Genet. 2009 Jul;5(7):e1000574. doi: 10.1371/journal.pgen.1000574. Epub 2009 Jul 24.PLoS Genet. 2009.PMID:19629165Free PMC article.
- The role of ISWI chromatin remodeling complexes in brain development and neurodevelopmental disorders.Goodwin LR, Picketts DJ.Goodwin LR, et al.Mol Cell Neurosci. 2018 Mar;87:55-64. doi: 10.1016/j.mcn.2017.10.008. Epub 2017 Dec 15.Mol Cell Neurosci. 2018.PMID:29249292Review.
- Opportunity knocks for uncovering the new function of an understudied nucleosome remodeling complex member, the bromodomain PHD finger transcription factor, BPTF.Zahid H, Olson NM, Pomerantz WCK.Zahid H, et al.Curr Opin Chem Biol. 2021 Aug;63:57-67. doi: 10.1016/j.cbpa.2021.02.003. Epub 2021 Mar 8.Curr Opin Chem Biol. 2021.PMID:33706239Free PMC article.Review.
Cited by
- G9a in Cancer: Mechanisms, Therapeutic Advancements, and Clinical Implications.Ni Y, Shi M, Liu L, Lin D, Zeng H, Ong C, Wang Y.Ni Y, et al.Cancers (Basel). 2024 Jun 8;16(12):2175. doi: 10.3390/cancers16122175.Cancers (Basel). 2024.PMID:38927881Free PMC article.Review.
- Nucleosome conformation dictates the histone code.Marunde MR, Fuchs HA, Burg JM, Popova IK, Vaidya A, Hall NW, Weinzapfel EN, Meiners MJ, Watson R, Gillespie ZB, Taylor HF, Mukhsinova L, Onuoha UC, Howard SA, Novitzky K, McAnarney ET, Krajewski K, Cowles MW, Cheek MA, Sun ZW, Venters BJ, Keogh MC, Musselman CA.Marunde MR, et al.Elife. 2024 Feb 6;13:e78866. doi: 10.7554/eLife.78866.Elife. 2024.PMID:38319148Free PMC article.
- Comparative genome-wide identification and characterization of SET domain-containing and JmjC domain-containing proteins in piroplasms.Liang Q, Zhang S, Liu Z, Wang J, Yin H, Guan G, You C.Liang Q, et al.BMC Genomics. 2024 Aug 26;25(1):804. doi: 10.1186/s12864-024-10731-2.BMC Genomics. 2024.PMID:39187768Free PMC article.
- Conserved molecular interactions within the HBO1 acetyltransferase complexes regulate cell proliferation.Avvakumov N, Lalonde ME, Saksouk N, Paquet E, Glass KC, Landry AJ, Doyon Y, Cayrou C, Robitaille GA, Richard DE, Yang XJ, Kutateladze TG, Côté J.Avvakumov N, et al.Mol Cell Biol. 2012 Feb;32(3):689-703. doi: 10.1128/MCB.06455-11. Epub 2011 Dec 5.Mol Cell Biol. 2012.PMID:22144582Free PMC article.
- The Dynamics of Histone Modifications during Mammalian Zygotic Genome Activation.Sotomayor-Lugo F, Iglesias-Barrameda N, Castillo-Aleman YM, Casado-Hernandez I, Villegas-Valverde CA, Bencomo-Hernandez AA, Ventura-Carmenate Y, Rivero-Jimenez RA.Sotomayor-Lugo F, et al.Int J Mol Sci. 2024 Jan 25;25(3):1459. doi: 10.3390/ijms25031459.Int J Mol Sci. 2024.PMID:38338738Free PMC article.Review.
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases