Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects
- PMID:16188237
- DOI: 10.1016/j.bbrc.2005.09.043
Cytochrome P450 CYP307A1/Spook: a regulator for ecdysone synthesis in insects
Abstract
The prothoracic gland (PG) has essential roles in synthesizing and secreting a steroid hormone called ecdysone that is critical for molting and metamorphosis of insects. However, little is known about the genes controlling ecdysteroidogenesis in the PG. To identify genes functioning in the PG of the silkworm, Bombyx mori, we used differential display PCR and focused on a cytochrome P450 gene designated Cyp307a1. Its expression level positively correlates with a change in the hemolymph ecdysteroid titer. In addition, Drosophila Cyp307a1 is encoded in the spook locus, one of the Halloween mutant family members showing a low ecdysone titer in vivo, suggesting that Cyp307a1 is involved in ecdysone synthesis. While Drosophila Cyp307a1 is expressed in the early embryos and adult ovaries, the expression is not observed in the PGs of embryos or third instar larvae. These results suggest a difference in the ecdysone synthesis pathways during larval development in these insects.
Similar articles
- Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera.Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho M, Warren JT, Marqués G, Shimell MJ, Gilbert LI, O'Connor MB.Ono H, et al.Dev Biol. 2006 Oct 15;298(2):555-70. doi: 10.1016/j.ydbio.2006.07.023. Epub 2006 Jul 29.Dev Biol. 2006.PMID:16949568
- Identification and expression profile of Halloween genes involved in ecdysteroid biosynthesis in Spodoptera littoralis.Iga M, Smagghe G.Iga M, et al.Peptides. 2010 Mar;31(3):456-67. doi: 10.1016/j.peptides.2009.08.002. Epub 2009 Aug 12.Peptides. 2010.PMID:19682519
- Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the 'Black Box' of the ecdysteroid biosynthesis pathway.Niwa R, Namiki T, Ito K, Shimada-Niwa Y, Kiuchi M, Kawaoka S, Kayukawa T, Banno Y, Fujimoto Y, Shigenobu S, Kobayashi S, Shimada T, Katsuma S, Shinoda T.Niwa R, et al.Development. 2010 Jun;137(12):1991-9. doi: 10.1242/dev.045641.Development. 2010.PMID:20501590
- The Halloween genes code for cytochrome P450 enzymes mediating synthesis of the insect moulting hormone.Rewitz KF, Rybczynski R, Warren JT, Gilbert LI.Rewitz KF, et al.Biochem Soc Trans. 2006 Dec;34(Pt 6):1256-60. doi: 10.1042/BST0341256.Biochem Soc Trans. 2006.PMID:17073797Review.
- A molecular genetic approach to the biosynthesis of the insect steroid molting hormone.Gilbert LI, Warren JT.Gilbert LI, et al.Vitam Horm. 2005;73:31-57. doi: 10.1016/S0083-6729(05)73002-8.Vitam Horm. 2005.PMID:16399407Review.
Cited by
- A deficit of detoxification enzymes: pesticide sensitivity and environmental response in the honeybee.Claudianos C, Ranson H, Johnson RM, Biswas S, Schuler MA, Berenbaum MR, Feyereisen R, Oakeshott JG.Claudianos C, et al.Insect Mol Biol. 2006 Oct;15(5):615-36. doi: 10.1111/j.1365-2583.2006.00672.x.Insect Mol Biol. 2006.PMID:17069637Free PMC article.
- The molecular evolution of cytochrome P450 genes within and between drosophila species.Good RT, Gramzow L, Battlay P, Sztal T, Batterham P, Robin C.Good RT, et al.Genome Biol Evol. 2014 Apr 20;6(5):1118-34. doi: 10.1093/gbe/evu083.Genome Biol Evol. 2014.PMID:24751979Free PMC article.
- Cooperative Control of Ecdysone Biosynthesis inDrosophila by Transcription Factors Séance, Ouija Board, and Molting Defective.Uryu O, Ou Q, Komura-Kawa T, Kamiyama T, Iga M, Syrzycka M, Hirota K, Kataoka H, Honda BM, King-Jones K, Niwa R.Uryu O, et al.Genetics. 2018 Feb;208(2):605-622. doi: 10.1534/genetics.117.300268. Epub 2017 Nov 29.Genetics. 2018.PMID:29187506Free PMC article.
- A cytochrome p450 conserved in insects is involved in cuticle formation.Sztal T, Chung H, Berger S, Currie PD, Batterham P, Daborn PJ.Sztal T, et al.PLoS One. 2012;7(5):e36544. doi: 10.1371/journal.pone.0036544. Epub 2012 May 4.PLoS One. 2012.PMID:22574182Free PMC article.
- TGFß/activin-dependent activation of Torso controls the timing of the metamorphic transition in the red flour beetle Tribolium castaneum.Chafino S, Salvia R, Cruz J, Martín D, Franch-Marro X.Chafino S, et al.PLoS Genet. 2023 Nov 27;19(11):e1010897. doi: 10.1371/journal.pgen.1010897. eCollection 2023 Nov.PLoS Genet. 2023.PMID:38011268Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Related information
LinkOut - more resources
Full Text Sources
Molecular Biology Databases