Translational regulation of GCN4 and the general amino acid control of yeast
- PMID:16153175
- DOI: 10.1146/annurev.micro.59.031805.133833
Translational regulation of GCN4 and the general amino acid control of yeast
Abstract
Cells reprogram gene expression in response to environmental changes by mobilizing transcriptional activators. The activator protein Gcn4 of the yeast Saccharomyces cerevisiae is regulated by an intricate translational control mechanism, which is the primary focus of this review, and also by the modulation of its stability in response to nutrient availability. Translation of GCN4 mRNA is derepressed in amino acid-deprived cells, leading to transcriptional induction of nearly all genes encoding amino acid biosynthetic enzymes. The trans-acting proteins that control GCN4 translation have general functions in the initiation of protein synthesis, or regulate the activities of initiation factors, so that the molecular events that induce GCN4 translation also reduce the rate of general protein synthesis. This dual regulatory response enables cells to limit their consumption of amino acids while diverting resources into amino acid biosynthesis in nutrient-poor environments. Remarkably, mammalian cells use the same strategy to downregulate protein synthesis while inducing transcriptional activators of stress-response genes under various stressful conditions, including amino acid starvation.
Similar articles
- Gln3-Gcn4 hybrid transcriptional activator determines catabolic and biosynthetic gene expression in the yeast Saccharomyces cerevisiae.Hernández H, Aranda C, Riego L, González A.Hernández H, et al.Biochem Biophys Res Commun. 2011 Jan 21;404(3):859-64. doi: 10.1016/j.bbrc.2010.12.075. Epub 2010 Dec 22.Biochem Biophys Res Commun. 2011.PMID:21184740
- Heterologous expression of membrane and soluble proteins derepresses GCN4 mRNA translation in the yeast Saccharomyces cerevisiae.Steffensen L, Pedersen PA.Steffensen L, et al.Eukaryot Cell. 2006 Feb;5(2):248-61. doi: 10.1128/EC.5.2.248-261.2006.Eukaryot Cell. 2006.PMID:16467466Free PMC article.
- A feedback circuit between transcriptional activation and self-destruction of Gcn4 separates its metabolic and morphogenic response in diploid yeasts.Herzog B, Streckfuss-Bömeke K, Braus GH.Herzog B, et al.J Mol Biol. 2011 Jan 28;405(4):909-25. doi: 10.1016/j.jmb.2010.11.033. Epub 2010 Nov 25.J Mol Biol. 2011.PMID:21111745
- Translational control of GCN4: an in vivo barometer of initiation-factor activity.Hinnebusch AG.Hinnebusch AG.Trends Biochem Sci. 1994 Oct;19(10):409-14. doi: 10.1016/0968-0004(94)90089-2.Trends Biochem Sci. 1994.PMID:7817398Review.
- The general control of amino acid biosynthetic genes in the yeast Saccharomyces cerevisiae.Hinnebusch AG.Hinnebusch AG.CRC Crit Rev Biochem. 1986;21(3):277-317. doi: 10.3109/10409238609113614.CRC Crit Rev Biochem. 1986.PMID:3536302Review.
Cited by
- A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation.Gottfried S, Koloamatangi SMBMJ, Daube C, Schiemann AH, Sattlegger E.Gottfried S, et al.PLoS One. 2022 Nov 28;17(11):e0277648. doi: 10.1371/journal.pone.0277648. eCollection 2022.PLoS One. 2022.PMID:36441697Free PMC article.
- Cytosolic and mitochondrial tRNA synthetase inhibitors increase lifespan in aGCN4/atf-4-dependent manner.Robbins CE, Patel B, Sawyer DL, Wilkinson B, Kennedy BK, McCormick MA.Robbins CE, et al.iScience. 2022 Oct 21;25(11):105410. doi: 10.1016/j.isci.2022.105410. eCollection 2022 Nov 18.iScience. 2022.PMID:36388960Free PMC article.
- Regulation of Translation Initiation under Biotic and Abiotic Stresses.Echevarría-Zomeño S, Yángüez E, Fernández-Bautista N, Castro-Sanz AB, Ferrando A, Castellano MM.Echevarría-Zomeño S, et al.Int J Mol Sci. 2013 Feb 26;14(3):4670-83. doi: 10.3390/ijms14034670.Int J Mol Sci. 2013.PMID:23443165Free PMC article.
- Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.Tanaka M, Yokoyama T, Saito H, Nishimoto M, Tsuda K, Sotta N, Shigematsu H, Shirouzu M, Iwasaki S, Ito T, Fujiwara T.Tanaka M, et al.Nat Chem Biol. 2024 May;20(5):605-614. doi: 10.1038/s41589-023-01513-0. Epub 2024 Jan 24.Nat Chem Biol. 2024.PMID:38267667
- Impact of uORFs in mediating regulation of translation in stress conditions.Moro SG, Hermans C, Ruiz-Orera J, Albà MM.Moro SG, et al.BMC Mol Cell Biol. 2021 May 16;22(1):29. doi: 10.1186/s12860-021-00363-9.BMC Mol Cell Biol. 2021.PMID:33992089Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases