doi: 10.1128/JB.187.16.5761-5766.2005.
The carbon-concentrating mechanism of the hydrothermal vent chemolithoautotroph Thiomicrospira crunogena
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- PMID:16077123
- PMCID: PMC1196061
- DOI: 10.1128/JB.187.16.5761-5766.2005
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The carbon-concentrating mechanism of the hydrothermal vent chemolithoautotroph Thiomicrospira crunogena
Kimberly P Dobrinski et al. J Bacteriol.2005 Aug.
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Steady-state growth rate constants (μ) and DIC concentrations forT. crunogena cells grown in chemostats under DIC limitation. Using the direct linear plot method (8), a rectangular hyperbola was generated to model the data (shown as a curve in the figure;KDIC = 0.22 mM; μmax = 0.44 h−1). The error bars indicate the standard error of the measurements (n = 3).
Carbon fixation rates forT. crunogena harvested from DIC-limited (solid circles) and DIC-sufficient (ammonia limited; open circles) chemostats with μ = 0.1 h−1. Data are presented with rectangular hyperbolae derived as in reference . For DIC-limited cells, theKDIC (0.026 mM) was lower than for DIC-sufficient cells (KDIC = 0.66 mM). TheVmax values for both were similar (133 and 120 nmol min−1 mg protein−1, respectively). The error bars indicate the standard deviations of the slopes.
Carbon fixation rates forT. crunogena harvested from DIC-limited chemostats with μ = 0.1 h−1, when supplied with CO2 (A) (KCO2 = 1.03 μM;Vmax = 97.2 nmol min−1 mg protein−1) or HCO3− (B) (KHCO3− = 53.6 μM;Vmax = 149 nmol min−1 mg protein−1). Data are presented with rectangular hyperbolae derived as in reference . In panel B, error bars indicate the standard deviations of the slopes. Error bars are not presented in panel A since the carbon fixation rates were based on two time points.
Intracellular DIC accumulation byT. crunogena cultivated under DIC limitation in a chemostat (DIC = 0.1 mM, μ = 0.1 h−1). A unity line (dashed; intracellular DIC = extracellular DIC) is presented with the data for comparison, and error bars are the standard errors of the intracellular concentrations (n = 3).
Energy dependence of intracellular DIC accumulation byT. crunogena. Cells were cultivated under DIC limitation with thiosulfate (Na2S2O3) as the electron donor. [DIC]i indicates the intracellular DIC concentration, [DIC]e indicates the extracellular DIC concentration (0.1 mM), and error bars indicate the standard error (n = 3).
References
- Badger, M., D. Hanson, and G. D. Price. 2002. Evolution and diversity of CO2 concentrating mechanisms in cyanobacteria. Funct. Plant Biol. 29:161-173. - PubMed
- Brocks, J. J., G. A. Logan, R. Buick, and R. E. Summons. 1999. Archean molecular fossils and the early rise of eukaryotes. Science 285:1033-1036. - PubMed
- Chain, P., J. Lamerdin, F. Larimer, W. Regala, V. Lao, M. Land, L. Hauser, A. Hooper, M. Klotz, J. Norton, L. Sayavedra-Soto, D. Arciero, N. Hommes, M. Whittaker, and D. Arp. 2003. Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotrophNitrosomonas europaea. J. Bacteriol. 185:2759-2773. - PMC - PubMed
- Childress, J. J. 1984. Metabolic and blood characteristics in the hydrothermal vent tube-wormRiftia pachyptila. Mar. Biol. 83:109-124.
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