B2 RNA binds directly to RNA polymerase II to repress transcript synthesis
- PMID:15300239
- DOI: 10.1038/nsmb812
B2 RNA binds directly to RNA polymerase II to repress transcript synthesis
Abstract
B2 RNA is a small noncoding RNA polymerase III transcript that represses mRNA transcription in response to heat shock in mouse cells. Here we define the mechanism by which B2 RNA inhibits RNA polymerase II (Pol II) transcription. Using a purified Pol II transcription system, we found that B2 RNA potently inhibits transcription by binding to core Pol II with high affinity and specificity. Through this interaction, B2 RNA assembles into preinitiation complexes at the promoter and blocks RNA synthesis. Once B2 RNA is removed from preinitiation complexes, transcriptional activity is restored. Our studies describe a previously unobserved mechanism of transcriptional repression by a small RNA and suggest that B2 RNA associates with Pol II at promoters in heat shocked cells to actively inhibit transcription.
Comment in
- RNA regulators of transcription.Wassarman KM.Wassarman KM.Nat Struct Mol Biol. 2004 Sep;11(9):803-4. doi: 10.1038/nsmb0904-803.Nat Struct Mol Biol. 2004.PMID:15332076No abstract available.
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