doi: 10.1016/S0014-5793(04)00351-5.
P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity
Affiliations
- PMID:15094067
- DOI: 10.1016/S0014-5793(04)00351-5
Item in Clipboard
P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity
Yvonne M Yannoni et al. FEBS Lett..
Free article Display options
Format
Display options
Format
Abstract
Three mitogen activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two-hybrid approach. ShcA, a signaling phospho-protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66(ShcA), and HPH2 was confirmed using co-immunoprecipitation. MK2 is activated with p66(ShcA) co-expression and p66(ShcA) is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66(Shc) in MK2 activation.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous