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.2004 Feb;70(2):913-20.
doi: 10.1128/AEM.70.2.913-920.2004.

Multi-virulence-locus sequence typing of Listeria monocytogenes

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Multi-virulence-locus sequence typing of Listeria monocytogenes

Wei Zhang et al. Appl Environ Microbiol.2004 Feb.

Erratum in

  • Appl Environ Microbiol. 2013 May;79(9):3146

Abstract

A multi-virulence-locus sequence typing (MVLST) scheme was developed for subtyping Listeria monocytogenes, and the results obtained using this scheme were compared to those of pulsed-field gel electrophoresis (PFGE) and the published results of other typing methods, including ribotyping (RT) and multilocus sequence typing (MLST). A set of 28 strains (eight different serotypes and three known genetic lineages) of L. monocytogenes was selected from a strain collection (n > 1,000 strains) to represent the genetic diversity of this species. Internal fragments (ca. 418 to 469 bp) of three virulence genes (prfA, inlB, and inlC) and three virulence-associated genes (dal, lisR, and clpP) were sequenced and analyzed. Multiple DNA sequence alignment identified 10 (prfA), 19 (inlB), 13 (dal), 10 (lisR), 17 (inlC), and 16 (clpP) allelic types and a total of 28 unique sequence types. Comparison of MVLST with automated EcoRI-RT and PFGE with ApaI enzymatic digestion showed that MVLST was able to differentiate strains that were indistinguishable by RT (13 ribotypes; discrimination index = 0.921) or PFGE (22 profiles; discrimination index = 0.970). Comparison of MVLST with housekeeping-gene-based MLST analysis showed that MVLST provided higher discriminatory power for serotype 1/2a and 4b strains than MLST. Cluster analysis based on the intragenic sequences of the selected virulence genes indicated a strain phylogeny closely related to serotypes and genetic lineages. In conclusion, MVLST may improve the discriminatory power of MLST and provide a convenient tool for studying the local epidemiology of L. monocytogenes.

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Figures

FIG. 1.
FIG. 1.
Genomic locations of the selected virulence loci inL. monocytogenes strain EGD-e. The six loci analyzed are shown in boldface characters.
FIG. 2.
FIG. 2.
(A) PFGE dendrogram constructed by the UPGMA. (B) Computer-normalized PFGE banding patterns withApaI enzymatic digestion.
FIG. 3.
FIG. 3.
N-J tree of 28L. monocytogenes strains; the tree was constructed on the basis of the number of nucleotide differences in the six virulence-gene fragments analyzed. Bootstrap values (1,000 replications) are shown at the interior branches.
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References

    1. Brosch, R., M. Brett, B. Catimel, J. B. Luchansky, B. Ojeniyi, and J. Rocourt. 1996. Genomic fingerprinting of 80 strains from the WHO. multicenter international typing study of Listeria monocytogenes via pulsed-field gel electrophoresis (PFGE). Int. J. Food Microbiol. 32:343-355. - PubMed
    1. Bruce, J. L., R. J. Hubner, E. M. Cole, C. I. McDowell, and J. A. Webster. 1995. Sets of EcoRI fragments containing ribosomal RNA sequences are conserved among different strains of Listeria monocytogenes. Proc. Natl. Acad. Sci. USA 92:5229-5233. - PMC - PubMed
    1. Cai, S., D. Y. Kabuki, A. Y. Kuaye, T. G. Cargioli, M. S. Chung, R. Nielsen, and M. Wiedmann. 2002. Rational design of DNA sequence-based strategies for subtyping Listeria monocytogenes. J. Clin. Microbiol. 40:3319-3325. - PMC - PubMed
    1. Centers for Disease Control and Prevention. 2001. Draft assessment of the relative risk to public health from food-borne Listeria monocytogenes among selected categories of ready-to-eat foods. [Online.]http://vm.cfsan.fda.gov/∼dms/lmrisk.html. Accessed 1 March 2003.
    1. Chan, M., M. C. J. Maiden, and B. G. Spratt. 2001. Database-driven multi locus sequence typing (MLST) of bacterial pathogens. Bioinformatics 17:1077-1083. - PubMed

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