Lack of evidence for reovirus infection in tissues from patients with biliary atresia and congenital dilatation of the bile duct
- PMID:14739089
- DOI: 10.1016/j.jhep.2003.10.025
Lack of evidence for reovirus infection in tissues from patients with biliary atresia and congenital dilatation of the bile duct
Abstract
Background/aims: To clarify the association between the reovirus infection of the hepatobiliary tree and the development of infantile obstructive cholangiopathy (IOC) including biliary atresia (BA) and congenital dilatation of the bile duct (CBD).
Methods: We designed reovirus common primers for nested RT-PCR based on the L3 gene segment. The spectrum and the sensitivity of common primers were evaluated with purified reoviral RNAs and reovirus mixed with stool samples. Then, nested RT-PCRs were performed with hepatobiliary and fecal samples obtained from patients with BA, CBD, and control diseases. Additionally, electron microscopy of stool samples was performed.
Results: The L3 common primers could amplify cDNAs synthesized from RNAs of three prototypes of reovirus, and detect as much as 5.0x10(3) plaque forming unit of serotype 3 Dearing strain in 100 mg of fecal samples. However, no amplification product was detected in 136 hepatobiliary tissues taken from 67 patients including 26 BAs and 28 CBDs, or in 65 fecal samples obtained from 15 patients including 10 BAs and 1 CBD. Additionally, viral particles were not found in any stool specimens by the electron microscope.
Conclusions: These data do not suggest that reoviruses play a major role in the etiology of IOC or BA.
Similar articles
- Detection of reovirus RNA in hepatobiliary tissues from patients with extrahepatic biliary atresia and choledochal cysts.Tyler KL, Sokol RJ, Oberhaus SM, Le M, Karrer FM, Narkewicz MR, Tyson RW, Murphy JR, Low R, Brown WR.Tyler KL, et al.Hepatology. 1998 Jun;27(6):1475-82. doi: 10.1002/hep.510270603.Hepatology. 1998.PMID:9620316
- Reovirus 3 not detected by reverse transcriptase-mediated polymerase chain reaction analysis of preserved tissue from infants with cholestatic liver disease.Steele MI, Marshall CM, Lloyd RE, Randolph VE.Steele MI, et al.Hepatology. 1995 Mar;21(3):697-702.Hepatology. 1995.PMID:7533124
- Detection of reovirus by reverse transcription-polymerase chain reaction using primers corresponding to conserved regions of the viral L1 genome segment.Leary TP, Erker JC, Chalmers ML, Wetzel JD, Desai SM, Mushahwar IK, Dermody TS.Leary TP, et al.J Virol Methods. 2002 Jul;104(2):161-5. doi: 10.1016/s0166-0934(02)00058-7.J Virol Methods. 2002.PMID:12088825
- Evidence for viral infection as a causative factor of human biliary atresia.Saito T, Terui K, Mitsunaga T, Nakata M, Ono S, Mise N, Yoshida H.Saito T, et al.J Pediatr Surg. 2015 Aug;50(8):1398-404. doi: 10.1016/j.jpedsurg.2015.04.006. Epub 2015 Apr 25.J Pediatr Surg. 2015.PMID:25979202Review.
- Etiology of biliary atresia as a developmental anomaly: recent advances.Nakamura K, Tanoue A.Nakamura K, et al.J Hepatobiliary Pancreat Sci. 2013 Jun;20(5):459-64. doi: 10.1007/s00534-013-0604-4.J Hepatobiliary Pancreat Sci. 2013.PMID:23567964Review.
Cited by
- Cytomegalovirus-specific T-cell reactivity in biliary atresia at the time of diagnosis is associated with deficits in regulatory T cells.Brindley SM, Lanham AM, Karrer FM, Tucker RM, Fontenot AP, Mack CL.Brindley SM, et al.Hepatology. 2012 Apr;55(4):1130-8. doi: 10.1002/hep.24807.Hepatology. 2012.PMID:22105891Free PMC article.
- Biliary Atresia: Clinical Phenotypes and Aetiological Heterogeneity.Davenport M, Muntean A, Hadzic N.Davenport M, et al.J Clin Med. 2021 Dec 1;10(23):5675. doi: 10.3390/jcm10235675.J Clin Med. 2021.PMID:34884377Free PMC article.Review.
- Toll-like receptors in secondary obstructive cholangiopathy.Miranda-Díaz AG, Alonso-Martínez H, Hernández-Ojeda J, Arias-Carvajal O, Rodríguez-Carrizalez AD, Román-Pintos LM.Miranda-Díaz AG, et al.Gastroenterol Res Pract. 2011;2011:265093. doi: 10.1155/2011/265093. Epub 2011 Oct 27.Gastroenterol Res Pract. 2011.PMID:22114589Free PMC article.
- Bone marrow mononuclear stem cell infusion improves biochemical parameters and scintigraphy in infants with biliary atresia.Sharma S, Kumar L, Mohanty S, Kumar R, Datta Gupta S, Gupta DK.Sharma S, et al.Pediatr Surg Int. 2011 Jan;27(1):81-9. doi: 10.1007/s00383-010-2712-4.Pediatr Surg Int. 2011.PMID:20857300
- Biliary innate immunity: function and modulation.Harada K, Nakanuma Y.Harada K, et al.Mediators Inflamm. 2010;2010:373878. doi: 10.1155/2010/373878. Epub 2010 Jul 27.Mediators Inflamm. 2010.PMID:20798866Free PMC article.Review.
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources