Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains
- PMID:14690610
- DOI: 10.1016/s1097-2765(03)00479-9
Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains
Abstract
The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains within euchromatin. In contrast, trimethylated H3 Lys9 was enriched at pericentric heterochromatin. Enzymes known to methylate H3 Lys9 displayed remarkably different enzymatic properties in vivo. G9a was responsible for all detectable H3 Lys9 dimethylation and a significant amount of monomethylation within silent euchromatin. In contrast, Suv39h1 and Suv39h2 directed H3 Lys9 trimethylation specifically at pericentric heterochromatin. Thus, different methylated states of H3 Lys9 are directed by specific histone methyltransferases to "mark" distinct domains of silent chromatin.
Similar articles
- Partitioning and plasticity of repressive histone methylation states in mammalian chromatin.Peters AH, Kubicek S, Mechtler K, O'Sullivan RJ, Derijck AA, Perez-Burgos L, Kohlmaier A, Opravil S, Tachibana M, Shinkai Y, Martens JH, Jenuwein T.Peters AH, et al.Mol Cell. 2003 Dec;12(6):1577-89. doi: 10.1016/s1097-2765(03)00477-5.Mol Cell. 2003.PMID:14690609
- G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis.Tachibana M, Sugimoto K, Nozaki M, Ueda J, Ohta T, Ohki M, Fukuda M, Takeda N, Niida H, Kato H, Shinkai Y.Tachibana M, et al.Genes Dev. 2002 Jul 15;16(14):1779-91. doi: 10.1101/gad.989402.Genes Dev. 2002.PMID:12130538Free PMC article.
- Catalytic properties and kinetic mechanism of human recombinant Lys-9 histone H3 methyltransferase SUV39H1: participation of the chromodomain in enzymatic catalysis.Chin HG, Patnaik D, Estève PO, Jacobsen SE, Pradhan S.Chin HG, et al.Biochemistry. 2006 Mar 14;45(10):3272-84. doi: 10.1021/bi051997r.Biochemistry. 2006.PMID:16519522
- The indexing potential of histone lysine methylation.Schotta G, Lachner M, Peters AH, Jenuwein T.Schotta G, et al.Novartis Found Symp. 2004;259:22-37; discussion 37-47, 163-9.Novartis Found Symp. 2004.PMID:15171245Review.
- The many faces of histone lysine methylation.Lachner M, Jenuwein T.Lachner M, et al.Curr Opin Cell Biol. 2002 Jun;14(3):286-98. doi: 10.1016/s0955-0674(02)00335-6.Curr Opin Cell Biol. 2002.PMID:12067650Review.
Cited by
- Genetic Aspects of Inflammation and Immune Response in Stroke.Nikolic D, Jankovic M, Petrovic B, Novakovic I.Nikolic D, et al.Int J Mol Sci. 2020 Oct 8;21(19):7409. doi: 10.3390/ijms21197409.Int J Mol Sci. 2020.PMID:33049931Free PMC article.Review.
- H3K9 Trimethylation Silences Fas Expression To Confer Colon Carcinoma Immune Escape and 5-Fluorouracil Chemoresistance.Paschall AV, Yang D, Lu C, Choi JH, Li X, Liu F, Figueroa M, Oberlies NH, Pearce C, Bollag WB, Nayak-Kapoor A, Liu K.Paschall AV, et al.J Immunol. 2015 Aug 15;195(4):1868-82. doi: 10.4049/jimmunol.1402243. Epub 2015 Jul 1.J Immunol. 2015.PMID:26136424Free PMC article.
- IκB kinase alpha and cancer.Liu S, Chen Z, Zhu F, Hu Y.Liu S, et al.J Interferon Cytokine Res. 2012 Apr;32(4):152-8. doi: 10.1089/jir.2011.0107. Epub 2011 Dec 7.J Interferon Cytokine Res. 2012.PMID:22149351Free PMC article.Review.
- Suz12 binds to silenced regions of the genome in a cell-type-specific manner.Squazzo SL, O'Geen H, Komashko VM, Krig SR, Jin VX, Jang SW, Margueron R, Reinberg D, Green R, Farnham PJ.Squazzo SL, et al.Genome Res. 2006 Jul;16(7):890-900. doi: 10.1101/gr.5306606. Epub 2006 Jun 2.Genome Res. 2006.PMID:16751344Free PMC article.
- Histone lysine methylation patterns in human cell types are arranged in distinct three-dimensional nuclear zones.Zinner R, Albiez H, Walter J, Peters AH, Cremer T, Cremer M.Zinner R, et al.Histochem Cell Biol. 2006 Jan;125(1-2):3-19. doi: 10.1007/s00418-005-0049-1. Epub 2005 Oct 8.Histochem Cell Biol. 2006.PMID:16215742
Publication types
MeSH terms
Substances
Related information
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases