MYCOBACTERIUM LEPRAE IN MICE: MINIMAL INFECTIOUS DOSE, RELATIONSHIP BETWEEN STAINING QUALITY AND INFECTIVITY, AND EFFECT OF CORTISONE
- PMID:14255702
- PMCID: PMC305516
- DOI: 10.1128/jb.89.2.365-372.1965
MYCOBACTERIUM LEPRAE IN MICE: MINIMAL INFECTIOUS DOSE, RELATIONSHIP BETWEEN STAINING QUALITY AND INFECTIVITY, AND EFFECT OF CORTISONE
Abstract
Shepard, Charles C. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), and Dorothy H. McRae. Mycobacterium leprae in mice: minimal infectious dose, relationship between staining quality and infectivity, and effect of cortisone. J. Bacteriol. 89:365-372. 1965.-The minimal infectious dose of Mycobacterium leprae in mouse foot pads was found to be on the order of 10 solidly staining bacilli. In a titration experiment, the actual number found was 3.4 to 34 solid bacilli, and the order of magnitude was confirmed by experience with inocula containing varying numbers of solidly staining leprosy bacilli from mouse passage and from clinical sources. The acid-fast staining quality of leprosy bacilli was related in a useful way to the subsequent rate at which bacillary growth appeared. When the proportion of solidly staining bacilli was high, the calculated generation time was shortest, and the lower the proportion, the longer the generation times. The results were in accord with the hypothesis that all viable bacilli are solid, and that when they die, most of them become nonsolid. Varying proportions of the dead bacilli, perhaps up to 10%, remain solid, at least temporarily. The growth curve of M. leprae in mice was followed in several experiments with total counts of acid-fast bacteria and determination of the ratio of solid bacilli. What had been called a maximal stationary phase was seen to consist of sequential phases of conversion of solid to nonsolid bacilli (death), reappearance of solid bacilli (growth), and conversion of solid to nonsolid bacilli (death). When cortisone was administered, leprosy bacilli grew somewhat more slowly during the logarithmic phase, but attained a higher level, especially of solidly staining bacilli.
Similar articles
- Relationship Between the Staining Quality of Mycobacterium leprae and Infectivity for Mice.McRae DH, Shepard CC.McRae DH, et al.Infect Immun. 1971 Jan;3(1):116-20. doi: 10.1128/iai.3.1.116-120.1971.Infect Immun. 1971.PMID:16557926Free PMC article.
- The effect of fixatives in the staining procedures for lepra bacilli in the tissues.LEON BLANCO F, FITE GL.LEON BLANCO F, et al.Int J Lepr. 1948 Apr-Jun;16(2):296.Int J Lepr. 1948.PMID:18877281No abstract available.
- THE BEHAVIOR OF BACILLUS LEPRAE IN COLD-BLOODED ANIMALS.Couret M.Couret M.J Exp Med. 1911 May 1;13(5):576-89. doi: 10.1084/jem.13.5.576.J Exp Med. 1911.PMID:19867440Free PMC article.
- Leprosy: A Review of Epidemiology, Clinical Diagnosis, and Management.Chen KH, Lin CY, Su SB, Chen KT.Chen KH, et al.J Trop Med. 2022 Jul 4;2022:8652062. doi: 10.1155/2022/8652062. eCollection 2022.J Trop Med. 2022.PMID:35832335Free PMC article.Review.
- The Brief Case: Strictly Anaerobic and Staining Acid Fast.Morton AB, Boyle E, Pettengill MA, Gancher E.Morton AB, et al.J Clin Microbiol. 2023 May 23;61(5):e0015022. doi: 10.1128/jcm.00150-22. Epub 2023 May 23.J Clin Microbiol. 2023.PMID:37219093Free PMC article.Review.No abstract available.
Cited by
- Immunological problems in leprosy research. 2.[No authors listed][No authors listed]Bull World Health Organ. 1973;48(4):483-94.Bull World Health Organ. 1973.PMID:4543552Free PMC article.
- Leprosy treatment: Can we replace opinions with research?Scollard DM.Scollard DM.PLoS Negl Trop Dis. 2020 Oct 8;14(10):e0008636. doi: 10.1371/journal.pntd.0008636. eCollection 2020 Oct.PLoS Negl Trop Dis. 2020.PMID:33031407Free PMC article.No abstract available.
- Effects of activated macrophages on Mycobacterium leprae.Ramasesh N, Adams LB, Franzblau SG, Krahenbuhl JL.Ramasesh N, et al.Infect Immun. 1991 Sep;59(9):2864-9. doi: 10.1128/iai.59.9.2864-2869.1991.Infect Immun. 1991.PMID:1908824Free PMC article.
- Mycobacterium leprae Transcriptome DuringIn Vivo Growth andEx Vivo Stationary Phases.Ojo O, Williams DL, Adams LB, Lahiri R.Ojo O, et al.Front Cell Infect Microbiol. 2022 Jan 12;11:817221. doi: 10.3389/fcimb.2021.817221. eCollection 2021.Front Cell Infect Microbiol. 2022.PMID:35096659Free PMC article.
- Viability of Mycobacterium leprae after multiplication in mice.Welch TM, Gelber RH, Murray LP, Ng H, O'Neill SM, Levy L.Welch TM, et al.Infect Immun. 1980 Nov;30(2):325-8. doi: 10.1128/iai.30.2.325-328.1980.Infect Immun. 1980.PMID:7002795Free PMC article.
References
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Miscellaneous