Evaluation of the estrogenic effects of legume extracts containing phytoestrogens
- PMID:12670155
- DOI: 10.1021/jf021114s
Evaluation of the estrogenic effects of legume extracts containing phytoestrogens
Abstract
Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.
Similar articles
- Requirement of metabolic activation for estrogenic activity of Pueraria mirifica.Lee YS, Park JS, Cho SD, Son JK, Cherdshewasart W, Kang KS.Lee YS, et al.J Vet Sci. 2002 Dec;3(4):273-7.J Vet Sci. 2002.PMID:12819377
- Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: an in vitro study in MCF-7 cells.Spagnuolo P, Rasini E, Luini A, Legnaro M, Luzzani M, Casareto E, Carreri M, Paracchini S, Marino F, Cosentino M.Spagnuolo P, et al.Fitoterapia. 2014 Apr;94:62-9. doi: 10.1016/j.fitote.2014.01.027. Epub 2014 Feb 5.Fitoterapia. 2014.PMID:24508860
- Estrogenic and antiestrogenic activities of flavonoid phytochemicals through estrogen receptor binding-dependent and -independent mechanisms.Collins-Burow BM, Burow ME, Duong BN, McLachlan JA.Collins-Burow BM, et al.Nutr Cancer. 2000;38(2):229-44. doi: 10.1207/S15327914NC382_13.Nutr Cancer. 2000.PMID:11525602
- Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy?Beck V, Rohr U, Jungbauer A.Beck V, et al.J Steroid Biochem Mol Biol. 2005 Apr;94(5):499-518. doi: 10.1016/j.jsbmb.2004.12.038. Epub 2005 Mar 23.J Steroid Biochem Mol Biol. 2005.PMID:15876415Review.
- [Phytoestrogens].Kinjo J.Kinjo J.Nihon Rinsho. 2000 Dec;58(12):2434-8.Nihon Rinsho. 2000.PMID:11187733Review.Japanese.
Cited by
- Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata.Li J, Li Z, Li C, Gou J, Zhang Y.Li J, et al.Plant Cell Rep. 2014 Jul;33(7):1173-85. doi: 10.1007/s00299-014-1606-7. Epub 2014 Apr 4.Plant Cell Rep. 2014.PMID:24700248
- Glyceollins as novel targeted therapeutic for the treatment of triple-negative breast cancer.Rhodes LV, Tilghman SL, Boue SM, Wang S, Khalili H, Muir SE, Bratton MR, Zhang Q, Wang G, Burow ME, Collins-Burow BM.Rhodes LV, et al.Oncol Lett. 2012 Jan;3(1):163-171. doi: 10.3892/ol.2011.460. Epub 2011 Oct 24.Oncol Lett. 2012.PMID:22740874Free PMC article.
- Environmental signaling and reproduction: a comparative biological and chemical perspective.McLachlan JA, Tilghman SL, Burow ME, Bratton MR.McLachlan JA, et al.Mol Cell Endocrinol. 2012 May 6;354(1-2):60-2. doi: 10.1016/j.mce.2011.11.028. Epub 2011 Dec 8.Mol Cell Endocrinol. 2012.PMID:22178089Free PMC article.Review.
- Analysis of the mechanisms underlying the endothelium-dependent antivasoconstriction of puerarin in rat aorta.Yan LP, Zhuang YL, Chan SW, Chen SL, Shi GG.Yan LP, et al.Naunyn Schmiedebergs Arch Pharmacol. 2009 Jun;379(6):587-97. doi: 10.1007/s00210-008-0388-2. Epub 2009 Feb 4.Naunyn Schmiedebergs Arch Pharmacol. 2009.PMID:19190894
- Antiosteoporotic activity of a syringic acid diet in ovariectomized mice.Tanaka T, Kawaguchi N, Zaima N, Moriyama T, Fukuta Y, Shirasaka N.Tanaka T, et al.J Nat Med. 2017 Oct;71(4):632-641. doi: 10.1007/s11418-017-1105-6. Epub 2017 Jul 5.J Nat Med. 2017.PMID:28681119
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous