N-terminal PDZ-binding domain in Kv1 potassium channels
- PMID:12435606
- DOI: 10.1016/s0014-5793(02)03572-x
N-terminal PDZ-binding domain in Kv1 potassium channels
Abstract
We have investigated the interactions of prototypical PDZ domains with both the C- and N-termini of Kv1.5 and other Kv channels. A combination of in vitro binding and yeast two-hybrid assays unexpectedly showed that PDZ domains derived from PSD95 bind both the C- and N-termini of the channels with comparable avidity. From doubly transfected HEK293 cells, Kv1.5 was found to co-immunoprecipitate with the PDZ protein, irrespective of the presence of the canonical C-terminal PDZ-binding motif in Kv1.5. Imaging analysis of the same HEK cell lines demonstrated that co-localization of Kv1.5 with PSD95 at the cell surface is similarly independent of the canonical PDZ-binding motif. Deletion analysis localized the N-terminal PDZ-binding site in Kv1.5 to the T1 region of the channel. Co-expression of PSD95 with Kv1.5 N- and C-terminal deletions in HEK cells had contrasting effects on the magnitudes of the potassium currents across the membranes of these cells. These findings may have important implications for the regulation of channel expression and function by PDZ proteins like PSD95.
Similar articles
- Neuronal inwardly rectifying K(+) channels differentially couple to PDZ proteins of the PSD-95/SAP90 family.Nehring RB, Wischmeyer E, Döring F, Veh RW, Sheng M, Karschin A.Nehring RB, et al.J Neurosci. 2000 Jan 1;20(1):156-62. doi: 10.1523/JNEUROSCI.20-01-00156.2000.J Neurosci. 2000.PMID:10627592Free PMC article.
- Amino-terminal determinants of U-type inactivation of voltage-gated K+ channels.Kurata HT, Soon GS, Eldstrom JR, Lu GW, Steele DF, Fedida D.Kurata HT, et al.J Biol Chem. 2002 Aug 9;277(32):29045-53. doi: 10.1074/jbc.M111470200. Epub 2002 May 20.J Biol Chem. 2002.PMID:12021261
- SAP97 increases Kv1.5 currents through an indirect N-terminal mechanism.Eldstrom J, Choi WS, Steele DF, Fedida D.Eldstrom J, et al.FEBS Lett. 2003 Jul 17;547(1-3):205-11. doi: 10.1016/s0014-5793(03)00668-9.FEBS Lett. 2003.PMID:12860415
- Domain structure and function of matrix metalloprotease 23 (MMP23): role in potassium channel trafficking.Galea CA, Nguyen HM, George Chandy K, Smith BJ, Norton RS.Galea CA, et al.Cell Mol Life Sci. 2014 Apr;71(7):1191-210. doi: 10.1007/s00018-013-1431-0. Epub 2013 Aug 3.Cell Mol Life Sci. 2014.PMID:23912897Free PMC article.Review.
- Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family.Gomperts SN.Gomperts SN.Cell. 1996 Mar 8;84(5):659-62. doi: 10.1016/s0092-8674(00)81043-0.Cell. 1996.PMID:8625403Review.No abstract available.
Cited by
- Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns.Burg ED, Platoshyn O, Tsigelny IF, Lozano-Ruiz B, Rana BK, Yuan JX.Burg ED, et al.Am J Physiol Cell Physiol. 2010 Mar;298(3):C496-509. doi: 10.1152/ajpcell.00464.2009. Epub 2009 Dec 16.Am J Physiol Cell Physiol. 2010.PMID:20018952Free PMC article.
- Channel properties of Nax expressed in neurons.Matsumoto M, Hiyama TY, Kuboyama K, Suzuki R, Fujikawa A, Noda M.Matsumoto M, et al.PLoS One. 2015 May 11;10(5):e0126109. doi: 10.1371/journal.pone.0126109. eCollection 2015.PLoS One. 2015.PMID:25961826Free PMC article.
- Post-synaptic density perturbs insulin-induced Kv1.3 channel modulation via a clustering mechanism involving the SH3 domain.Marks DR, Fadool DA.Marks DR, et al.J Neurochem. 2007 Nov;103(4):1608-27. doi: 10.1111/j.1471-4159.2007.04870.x. Epub 2007 Sep 13.J Neurochem. 2007.PMID:17854350Free PMC article.
- A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding.Lenfant N, Polanowska J, Bamps S, Omi S, Borg JP, Reboul J.Lenfant N, et al.BMC Genomics. 2010 Nov 26;11:671. doi: 10.1186/1471-2164-11-671.BMC Genomics. 2010.PMID:21110867Free PMC article.
- Characterization of diverse internal binding specificities of PDZ domains by yeast two-hybrid screening of a special peptide library.Mu Y, Cai P, Hu S, Ma S, Gao Y.Mu Y, et al.PLoS One. 2014 Feb 4;9(2):e88286. doi: 10.1371/journal.pone.0088286. eCollection 2014.PLoS One. 2014.PMID:24505465Free PMC article.
MeSH terms
Substances
Related information
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous