Increased viability of transplanted hepatocytes when hepatocytes are co-encapsulated with bone marrow stem cells using a novel method
- PMID:12027231
- DOI: 10.1081/bio-120003191
Increased viability of transplanted hepatocytes when hepatocytes are co-encapsulated with bone marrow stem cells using a novel method
Abstract
This study is to investigate the viability of hepatocytes when transplanted into Wistar rats using co-encapsulated hepatocytes and bone marrow stem cells. Hepatocytes and bone marrow stem cells, isolated from Wistar rats, are co-encapsulated using either the standard single-step method or a novel two-step cell encapsulation method (www.artcell.mcgill.ca). After intraperitoneal transplantation into Wistar rats, the histology, fate of recovered microcapsules and viability of encapsulated hepatocytes are studied. When prepared using the standard method, there is excellent viability but only for up to 3 weeks. After this, there is extensive fibrous coating and severe fibrous adhesion and no microcapsules can be recovered. On the other hand, using the new two-step encapsulation method, the viability of the encapsulated hepatocytes can be followed for more than 4 months after transplantation. Even up to 4 months, there is significantly less host reaction when using the two-step encapsulation method and 50% of the microcapsules can be recovered. Co-encapsulated with bone marrow stem cells resulted in further increase in viability of the hepatocytes when followed up to 4 months after transplantation. This new approach may improve the potential feasibility of using co-encapsulation of hepatocytes and bone marrow stem cells in bio-artificial liver support for the treatment of liver failure, especially for acute liver failure.
Similar articles
- Effects of bone marrow cells on hepatocytes: when co-cultured or co-encapsulated together.Liu Z, Chang TM.Liu Z, et al.Artif Cells Blood Substit Immobil Biotechnol. 2000 Jul;28(4):365-74. doi: 10.3109/10731190009119365.Artif Cells Blood Substit Immobil Biotechnol. 2000.PMID:10928705
- Coencapsulation of hepatocytes and bone marrow stem cells: in vitro conversion of ammonia and in vivo lowering of bilirubin in hyperbilirubemia Gunn rats.Liu ZC, Chang TM.Liu ZC, et al.Int J Artif Organs. 2003 Jun;26(6):491-7. doi: 10.1177/039139880302600607.Int J Artif Organs. 2003.PMID:12894754
- [Sustainment of hepatocyte function with mixed cellular co-encapsulation].Wang YF, Xue YL, Nan X, Liang F, Luo Y, Li YL, Gao YH, Yue W, Pei XT.Wang YF, et al.Zhonghua Yi Xue Za Zhi. 2005 Sep 14;85(35):2481-6.Zhonghua Yi Xue Za Zhi. 2005.PMID:16321274Chinese.
- Coencapsulation of hepatocytes and bone marrow cells: in vitro and in vivo studies.Chang Liu Z, Chang TM.Chang Liu Z, et al.Biotechnol Annu Rev. 2006;12:137-51. doi: 10.1016/S1387-2656(06)12005-0.Biotechnol Annu Rev. 2006.PMID:17045194Review.
- Therapeutic potential of bone marrow stem cells for liver diseases.Popp FC, Piso P, Schlitt HJ, Dahlke MH.Popp FC, et al.Curr Stem Cell Res Ther. 2006 Sep;1(3):411-8. doi: 10.2174/157488806778226759.Curr Stem Cell Res Ther. 2006.PMID:18220884Review.
Cited by
- Stem Cell-Based Strategies: The Future Direction of Bioartificial Liver Development.Feng L, Wang Y, Fu Y, Li T, He G.Feng L, et al.Stem Cell Rev Rep. 2024 Apr;20(3):601-616. doi: 10.1007/s12015-023-10672-5. Epub 2024 Jan 3.Stem Cell Rev Rep. 2024.PMID:38170319Review.
- Hepatic stem cells: existence and origin.Zhang Y, Bai XF, Huang CX.Zhang Y, et al.World J Gastroenterol. 2003 Feb;9(2):201-4. doi: 10.3748/wjg.v9.i2.201.World J Gastroenterol. 2003.PMID:12532431Free PMC article.Review.
- Artificial cell microencapsulated stem cells in regenerative medicine, tissue engineering and cell therapy.Liu ZC, Chang TM.Liu ZC, et al.Adv Exp Med Biol. 2010;670:68-79. doi: 10.1007/978-1-4419-5786-3_7.Adv Exp Med Biol. 2010.PMID:20384219Free PMC article.Review.
- Effects of coencapsulation of hepatocytes with adipose-derived stem cells in the treatment of rats with acute-on-chronic liver failure.Zhang Y, Chen XM, Sun DL.Zhang Y, et al.Int J Artif Organs. 2014 Feb;37(2):133-41. doi: 10.5301/ijao.5000284. Epub 2014 Feb 7.Int J Artif Organs. 2014.PMID:24619896Free PMC article.
- The osteogenic differentiation of human dental pulp stem cells in alginate-gelatin/Nano-hydroxyapatite microcapsules.Alipour M, Firouzi N, Aghazadeh Z, Samiei M, Montazersaheb S, Khoshfetrat AB, Aghazadeh M.Alipour M, et al.BMC Biotechnol. 2021 Jan 11;21(1):6. doi: 10.1186/s12896-020-00666-3.BMC Biotechnol. 2021.PMID:33430842Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical