doi: 10.1006/bbrc.2001.5472.
IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis
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- PMID:11520071
- DOI: 10.1006/bbrc.2001.5472
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IsCT, a novel cytotoxic linear peptide from scorpion Opisthacanthus madagascariensis
L Dai et al. Biochem Biophys Res Commun..
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Abstract
A novel cytotoxic linear peptide, IsCT, was characterized from scorpion Opisthacanthus madagascariensis. It is a linear peptide with a molecular weight of 1501.9 Da composed of 13 amino acid residues without cysteines. MS/MS analysis showed that its C-terminal is amidated. The identity of IsCT is re-confirmed by comparing the chemical synthesized peptide with the natural one. IsCT demonstrated antimicrobial activity against both gram-positive and gram-negative bacteria and hemolytic activity to sheep red blood cells. Also, it can release histamine from rat peritoneal mast cells. The CD absorption suggested that IsCT had an alpha-helix configuration in aqueous TFE. IsCT is one of the shortest natural cytotoxic peptides described, and it will be a suitable model for studying peptide-lipid interactions.
Copyright 2001 Academic Press.
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