Multiplex detection of hotspot mutations by rolling circle-enabled universal microarrays
- PMID:11502859
- DOI: 10.1038/labinvest.3780320
Multiplex detection of hotspot mutations by rolling circle-enabled universal microarrays
Erratum in
- Lab Invest 2001 Oct;81(10):1338
Abstract
Detection of somatic low abundance mutations in early cancer development requires a discriminatory, specific, and high-throughput methodology. In this study we report specific, discriminatory detection of low abundance mutations through a novel combination of rolling circle amplification (Nat Genet 1998; 19:225-232) and PCR ligation detection reaction on a universal oligonucleotide microarray (J Mol Biol 1999; 292:251-262). After mutation-specific multiplex ligation and hybridization of 17 pairs of probes to a generic microarray, the ligated probes were visualized. The multiplex mutation-specific ligation is possible only because rolling circle amplification permits quantification of previously undetectable hybridization events conducive to the detection of a single mutation from within a pool of over 100 wild-type alleles. This system is readily adaptable to high-throughput automation using a robot such as the Biomek platform.
Similar articles
- [Development of multiplex oligonucleotide ligation-PCR-universal DNA microarrays for detection of foodborne pathogens].Wang X, Ying S, Han R, Yuan J.Wang X, et al.Wei Sheng Yan Jiu. 2017 Mar;46(2):225-231.Wei Sheng Yan Jiu. 2017.PMID:29903098Chinese.
- Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.Milbury CA, Chen CC, Mamon H, Liu P, Santagata S, Makrigiorgos GM.Milbury CA, et al.J Mol Diagn. 2011 Mar;13(2):220-32. doi: 10.1016/j.jmoldx.2010.10.008.J Mol Diagn. 2011.PMID:21354058Free PMC article.
- [Detection of single base polymorphism in p53 gene by ligase detection reaction and rolling circle amplification on microarrays].Kashkin KN, Strizhkov BN, Griadunov DA, Surzhikov SA, Grechishnikova IV, Kreĭndlin EIa, Chupeeva VV, Evseev KB, Turygin AIu, Mirzabekov AD.Kashkin KN, et al.Mol Biol (Mosk). 2005 Jan-Feb;39(1):30-9.Mol Biol (Mosk). 2005.PMID:15773545Russian.
- Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies.Shi MM.Shi MM.Clin Chem. 2001 Feb;47(2):164-72.Clin Chem. 2001.PMID:11159763Review.
- COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.Li J, Makrigiorgos GM.Li J, et al.Biochem Soc Trans. 2009 Apr;37(Pt 2):427-32. doi: 10.1042/BST0370427.Biochem Soc Trans. 2009.PMID:19290875Review.
Cited by
- Multiplex polymerase chain reaction: a practical approach.Markoulatos P, Siafakas N, Moncany M.Markoulatos P, et al.J Clin Lab Anal. 2002;16(1):47-51. doi: 10.1002/jcla.2058.J Clin Lab Anal. 2002.PMID:11835531Free PMC article.Review.
- Mutational load distribution analysis yields metrics reflecting genetic instability during pancreatic carcinogenesis.Tarafa G, Tuck D, Ladner D, Topazian M, Brand R, Deters C, Moreno V, Capella G, Lynch H, Lizardi P, Costa J.Tarafa G, et al.Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4306-11. doi: 10.1073/pnas.0708250105. Epub 2008 Mar 12.Proc Natl Acad Sci U S A. 2008.PMID:18337498Free PMC article.
- High-throughput SNP genotyping.Jenkins S, Gibson N.Jenkins S, et al.Comp Funct Genomics. 2002;3(1):57-66. doi: 10.1002/cfg.130.Comp Funct Genomics. 2002.PMID:18628885Free PMC article.
- Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements.Zhou H, Bouwman K, Schotanus M, Verweij C, Marrero JA, Dillon D, Costa J, Lizardi P, Haab BB.Zhou H, et al.Genome Biol. 2004;5(4):R28. doi: 10.1186/gb-2004-5-4-r28. Epub 2004 Mar 30.Genome Biol. 2004.PMID:15059261Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources