The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp
- PMID:11118601
- DOI: 10.1016/s0141-0229(00)00274-x
The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp
Abstract
The ascomycete, Botryosphaeria sp, produced two extracellular constitutive laccases (PPO-I and PPO-II) active toward the substrates: 2, 2(1)-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) [ABTS], and 2,6-dimethoxyphenol (DMP), respectively. The production of both laccases increased when the fungal isolate was grown in the presence of veratryl alcohol, and resulted in optimal laccase production (100- and 25- fold, respectively) at 40 mM. The effect of aeration on growth and laccase production was studied in baffled flasks, and showed that aeration of the cultures increased the production of both enzymes 4-5 fold in the presence of veratryl alcohol. Both laccases were susceptible to inhibition by azide, acetate and chloride anions. Veratryl alcohol inhibited the laccase-catalyzed polymerization of DMP. Growing cultures of Botryosphaeria sp. produced an exopolysaccharide of the beta-glucan type whose synthesis was depressed when grown in the presence of veratryl alcohol.
Similar articles
- The effect of carbohydrate carbon sources on the production of constitutive and inducible laccases by Botryosphaeria sp.Alves da Cunha MA, Barbosa AM, Giese EC, Dekker RF.Alves da Cunha MA, et al.J Basic Microbiol. 2003;43(5):385-92. doi: 10.1002/jobm.200310250.J Basic Microbiol. 2003.PMID:12964181
- Growth and production of laccases by the ligninolytic fungi, Pleurotus ostreatus and Botryosphaeria rhodina , cultured on basal medium containing the herbicide, Scepter (imazaquin).Rezende MI, Barbosa AM, Vasconcelos AF, Haddad R, Dekker RF.Rezende MI, et al.J Basic Microbiol. 2005;45(6):460-9. doi: 10.1002/jobm.200410552.J Basic Microbiol. 2005.PMID:16304708
- Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05.Dekker RF, Barbosa AM, Giese EC, Godoy SD, Covizzi LG.Dekker RF, et al.Int Microbiol. 2007 Sep;10(3):177-85.Int Microbiol. 2007.PMID:18075999
- Transcriptional analysis of the laccase-like gene from Burkholderia cepacia BNS and expression in Escherichia coli.Ma Y, Li L, Tian H, Lu M, Megharaj M, He W.Ma Y, et al.Appl Microbiol Biotechnol. 2019 Jan;103(2):747-760. doi: 10.1007/s00253-018-9468-5. Epub 2018 Oct 26.Appl Microbiol Biotechnol. 2019.PMID:30362077
- Recent developments and applications of immobilized laccase.Fernández-Fernández M, Sanromán MÁ, Moldes D.Fernández-Fernández M, et al.Biotechnol Adv. 2013 Dec;31(8):1808-25. doi: 10.1016/j.biotechadv.2012.02.013. Epub 2012 Feb 28.Biotechnol Adv. 2013.PMID:22398306Review.
Cited by
- Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye.Hao J, Song F, Huang F, Yang C, Zhang Z, Zheng Y, Tian X.Hao J, et al.J Ind Microbiol Biotechnol. 2007 Mar;34(3):233-40. doi: 10.1007/s10295-006-0191-3. Epub 2006 Dec 15.J Ind Microbiol Biotechnol. 2007.PMID:17171552
- Mycoremediation of endosulfan and its metabolites in aqueous medium and soil by Botryosphaeria laricina JAS6 and Aspergillus tamarii JAS9.Silambarasan S, Abraham J.Silambarasan S, et al.PLoS One. 2013 Oct 10;8(10):e77170. doi: 10.1371/journal.pone.0077170. eCollection 2013.PLoS One. 2013.PMID:24130848Free PMC article.
- Fungal laccases and their applications in bioremediation.Viswanath B, Rajesh B, Janardhan A, Kumar AP, Narasimha G.Viswanath B, et al.Enzyme Res. 2014;2014:163242. doi: 10.1155/2014/163242. Epub 2014 May 15.Enzyme Res. 2014.PMID:24959348Free PMC article.Review.
- Laccase Activity and Azo Dye Decolorization Potential ofPodoscypha elegans.Pramanik S, Chaudhuri S.Pramanik S, et al.Mycobiology. 2018 Mar 28;46(1):79-83. doi: 10.1080/12298093.2018.1454006. eCollection 2018.Mycobiology. 2018.PMID:29998036Free PMC article.
- Influence of the carbon source on the growth and lignocellulolytic enzyme production by Morchella esculenta strains.Papinutti L, Lechner B.Papinutti L, et al.J Ind Microbiol Biotechnol. 2008 Dec;35(12):1715-21. doi: 10.1007/s10295-008-0464-0. Epub 2008 Sep 13.J Ind Microbiol Biotechnol. 2008.PMID:18791758
LinkOut - more resources
Full Text Sources
Miscellaneous