Serological detection of infection with diverse human and simian immunodeficiency viruses using consensus env peptides
- PMID:10882678
- PMCID: PMC95940
- DOI: 10.1128/CDLI.7.4.706-709.2000
Serological detection of infection with diverse human and simian immunodeficiency viruses using consensus env peptides
Abstract
Cross-species transmission has been shown to play an important role in the emergence of human retroviruses. We developed a generic enzyme immunoassay using synthetic peptides from gp41 and C2V3 consensus sequences (human immunodeficiency virus [HIV] type 1 [HIV-1] groups M, O, and N and the homologous region of simian immunodeficiency virus [SIV] strains from chimpanzees [SIVcpz], SIVcpzGAB1 and SIVcpzANT) to detect divergent HIV and SIV. A cocktail of peptides from gp41 and C2V3 (M-O) detected all HIV-1 group M and O sera and showed cross-reactivity with SIVcpz sera. Further, a mixture of C2V3 peptides (GAB1-ANT) failed to detect HIV-1 infections but reacted with all SIVcpz sera, allowing discrimination of SIVcpz from HIV-1 infections. Since most SIVcpz sera cross-reacted with HIV-1 peptides, we next evaluated SIVcpz serum reactivity with rapid tests for HIV-1/2. SIVcpzANT and SIVcpzUS sera reacted with the Sero-strip and Multispot assays. Both tests are sensitive in detecting group M (97 100%, respectively), although Multispot has lower sensitivity for group O detection (67%) than does Sero-strip (100%). The limited volume and time required to perform these assays make them a generic tool for field screening. The env peptide-based assay and rapid tests should allow for the identification of emerging variants of HIV and SIV.
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References
- Constantine N T, Zekeng L, Sangare A K, Gurtler L, Saville R I, Anhary H, Wild C. Diagnostic challenges for rapid human immunodeficiency virus assays: performance using HIV-1 group O, HIV-1 group M, and HIV-2 samples. J Hum Virol. 1997;1:45–51. - PubMed
- Dorn J, Masciotra S, Yang C, Downing R, Biryawaho B, Mastro T M, Nkengasong J, Pieniazek D, Rayfield M A, Hu D J, Lal R B. Analysis of genetic variability within the immunodominant epitopes of envelope gp41 from human immunodeficiency virus type 1 (HIV-1) group M and its impact on HIV-1 antibody detection. J Clin Microbiol. 2000;38:773–780. - PMC - PubMed
- Gao F, Bailes E, Robertson D L, Chen Y, Rodenburg C M, Michael S F, Cummins L B, Arthur L O, Peeters M, Shaw G M, Sharp P M, Hahn B H. Origin of HIV-1 in the chimpanzee Pan troglodytes troglodytes. Nature. 1999;397:436–441. - PubMed
- Hu D, Dondero T J, Mastro T D, Gayle H D. Global and molecular epidemiology of HIV. In: Wonnser G P, editor. AIDS and other manifestations of HIV infection. Philadelphia, Pa: Lipponcott-Raven Publishers; 1998. pp. 27–40.
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