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An integrated hog futures price forecasting model based on whale optimization algorithm, LightGBM, and Complete Ensemble Empirical Mode Decomposition with Adaptive Noise is proposed to overcome the limitations of a single machine learning model with low prediction accuracy and insufficient model stability. The simulation process begins with a grey correlation analysis of the hog futures price index system in order to identify influencing factors; after that, the WOA-LightGBM model is developed, and the WOA algorithm is used to optimize the LightGBM (...) model parameters; and, finally, the residual sequence is decomposed and corrected by using the CEEMDAN method to build a combined WOA-LightGBM-CEEMDAN model. Furthermore, it is used for comparison experiments to check the validity of the model by using data from CSI 300 stock index futures. Based on all experimental results, the proposed combined model shows the highest prediction accuracy, surpassing the comparative model. The model proposed in this study is accurate enough to meet the forecasting accuracy requirements and provides an effective method for forecasting future prices. (shrink)

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We study an application of gaggle theory to unary negative modal operators. First we treat negation as impossibility and get a minimal logic system Ki that has a perp semantics. Dunn 's kite of different negations can be dealt with in the extensions of this basic logic Ki. Next we treat negation as “unnecessity” and use a characteristic semantics for different negations in a kite which is dual to Dunn 's original one. Ku is the minimal logic that has a (...) characteristic semantics. We also show that Shramko's falsification logic FL can be incorporated into some extension of this basic logic Ku. Finally, we unite the two basic logics Ki and Ku together to get a negative modal logic K-, which is dual to the positive modal logic K+ in [7]. Shramko has suggested an extension of Dunn 's kite and also a dual version in [12]. He also suggested combining them into a “united” kite. We give a united semantics for this united kite of negations. (shrink)

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Due to the outbreak of the Coronavirus Disease-2019 pandemic and consequent confinement measures, young people are vulnerable to mental health problems. The current study compared a group of 440 young adolescents and a group of 330 emerging adults to investigate the extent to which perceived social support and psychological capital were differentially associated with mental health problems. Participants were asked to report their current psychosocial adaptation status during the COVID-19 pandemic, and data were collected via online questionnaires during a relatively (...) severe period of COVID-19 in China. Results of the multi-group path analysis indicated that the effect of perceived social support on mental health problems was mediated by PsyCap for young adolescents, but not for emerging adults. These results were discussed with respect to the mechanism of how social support and PsyCap serve as protective mental health factors for youth in the context of the COVID-19 pandemic. (shrink)

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Should studies of social history engage in questions of important socio-political changes? The answer is undoubtedly affirmative. So far as the important changes in 19th century China are concerned, some case studies in regional social history have presented a picture of greater complexity and diversity. The Ningbo case, for example, shows that when a port that has had a history of foreign trade becomes embroiled in conflict between Chinese and foreigners, this conflict may simply become one mode of interaction amongst (...) many between Chinese and foreigners. The case of Turmot is an important turning point in Chinese history, since it marks the reversal of 2000 years of frequent attempts by nomadic tribes to expand southward. This trend is completely reversed by the Han nationalities’ northward immigration and reclamation, a development as significant as the Western invasion of China in terms of consequent social transformations. These historical traces revealed from within regions deepen our comprehension of these social transformations; consequently, such studies in social history may manage to provide answers different from those offered by the traditional model of political history. (shrink)

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This paper investigates the fixed-time coordinated control problem of six-degree-of-freedom dynamic model for multiple spacecraft formation flying with input quantization, where the communication topology is assumed directed. Firstly, a new multispacecraft nonsingular fixed-time terminal sliding mode vector is derived by using neighborhood state information. Secondly, a hysteretic quantizer is utilized to quantify control force and torque. Utilizing such a quantizer not only can reduce the required communication rate but also can eliminate the control chattering phenomenon induced by the logarithmic quantizer. (...) Thirdly, a 6-DOF fixed-time coordinated control strategy with adaptive tuning laws is proposed, such that the practical fixed-time stability of the controlled system is ensured in the presence of both upper bounds of unknown external disturbances. It theoretically proves that the relative tracking errors of attitude and position can converge into the regions in a fixed time. Finally, a numerical example is exploited to show the usefulness of the theoretical results. (shrink)

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This study examined the extent to which the impact of green transformational leadership on employee green behavior through follower perceptions of value congruence. Path analyzing on data from 193 subordinate-leader dyads showed that followers’ value congruence with their leader mediated the effects of green transformational leadership on employee green behavior. Results also supported that green identity moderated the indirect effect of green transformational leadership on employee green behavior through value congruence, such that the indirect effect was more positive when green (...) identity was high than when it was low. These findings provided valuable contribution to green transformational leadership, value congruence and employee green behavior by exploring the relationship between them. Practical implications and directions for future research are also discussed. (shrink)

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This research examines how and why reactions to other consumers’ unethical behavior differ among consumers and vary in different situations. Drawing on construal level theory, the authors propose that the relationship between other consumers’ unethical behavior and focal consumers’ unethical behavior is moderated by focal consumers’ construal level, and self-expressiveness mediates this moderating effect. Specifically, consumers at higher construal levels tend to view their behavior as more self-expressive and are thus less likely to imitate other consumers’ unethical behavior. Study 1 (...) indicates that focal consumers who have a chronic tendency to construe information at high levels are less likely to replicate other consumers’ unethical behavior than those who have a chronic tendency to construe information at low levels. Studies 2, 3a and 3b show that when primed with higher-level construals, consumers are less likely to follow other consumers’ unethical behavior than when primed with lower-level construals. The mediating effect of self-expressiveness was tested and supported in Study 1 and Study 3b. These findings help to better understand consumers’ reactions to other consumers’ unethical behavior. (shrink)

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Despite the prevalent role of guanxi in conducting business in Chinese, it is unclear whether interpersonal guanxi fosters interfirm trust. Taking a contingency approach, this study examines how institutional (government–market relationship and Buddhism influence) and individual (relative role performance and the span of partner control) factors moderate the association between interpersonal guanxi and interfirm trust. Based on a paired survey between salespersons and sales managers and two secondary datasets, this study finds that interpersonal guanxi is positively associated with interfirm trust. (...) Moreover, this positive effect is stronger when firms operate in regions with strong government–market relationships and strong Buddhism influence. In addition, the positive relationship is weaker when guanxi resides in boundary spanners who have relatively high role performance, but becomes stronger when boundary spanners have a broad span of partner control. These findings highlight the situations in which guanxi at an interpersonal level is more or less likely to foster interfirm trust. (shrink)

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Previous studies have found a link between red and aggressive behavior. For example, athletes who wear red uniforms in sports are considered to have a competitive advantage. So far, most previous studies have adopted self-report methods, which have low face validity and were easily influenced by the social expectations. Therefore, the study used two implicit methods to further explore the association between red and aggressiveness. A modified Stroop task was used in Experiment 1 to probe college students’ differences between “congruent” (...) tasks and “incongruent” tasks. Result showed that participants responded more quickly to the congruent tasks than the incongruent tasks. Then, in order to adapt to the competitive context, Experiment 2 used an implicit association test with photos of athletes as the stimulus to college students and athletes to evaluate “congruent” tasks as well as “incongruent” tasks, respectively. According to the results, both college students and athletes respond faster to congruent tasks than to incongruent tasks. Besides, athletes’ reactions to the red–aggressiveness association are faster than college students, which may relate to the athletes’ professional experience. The athletes may be more aggressive and impulsive. Overall, the study has attempted to examine the association between red and aggressiveness through implicit methods, but in the future, researches are need to find a deep association from brain mechanism aspect. (shrink)

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Many actions have instrumental aims, in which we move our bodies to achieve a physical outcome in the environment. However, we also perform actions with epistemic aims, in which we move our bodies to acquire information and learn about the world. A large literature on action recognition investigates how observers represent and understand the former class of actions; but what about the latter class? Can one person tell, just by observing another person’s movements, what they are trying to learn? Here, (...) 5 experiments explore epistemic action understanding. We filmed volunteers playing a ‘physics game’ consisting of two rounds: Players shook an opaque box and attempted to determine (i) the number of objects hidden inside, or (ii) the shape of the objects inside. Then, independent subjects watched these videos and were asked to determine which videos came from which round: Who was shaking for number and who was shaking for shape? Across several variations, observers successfully determined what an actor was trying to learn, based only on their actions (i.e., how they shook the box)—even when the box’s contents were identical across rounds. These results demonstrate that humans can infer epistemic intent from physical behaviors, adding a new dimension to research on action understanding. (shrink)

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Motivated by a large literature on how firm-specific resources drive firm performance, we propose and find that heterogeneity in investor optimism regarding firm-specific attributes plays a very important role in influencing the managerial propensity to manipulate financial statements. When firm-level investor optimism is moderate, the incidence of accounting misconduct increases, but it decreases when investors are highly optimistic. Further, market reaction to the announcement of financial restatements is more negative when investors held more optimistic firm-specific beliefs at the time of (...) initial misstatement. These findings are robust to alternative firm-specific optimism measures linked to analysts, general investors and unsophisticated individual investors, controls for market-wide consumer sentiment unexplained by macroeconomic factors, economy-wide and industry-level optimism, potential selection bias and reverse causality. Our analysis highlights the importance of firm-level investor optimism in predicting, preventing and detecting accounting misconduct. (shrink)

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This study examined the role of individuals’ cognitive flexibility and self-monitoring in shaping their workplace advice network centrality. Drawing on advice network generation theory, we hypothesized a positive relationship between cognitive flexibility and advice network centrality, and a moderation effect of self-monitoring on this relationship. Then, we collected two time-points data from insurance salesmen to test the hypotheses. As predicted, cognitive flexibility was positively associated with advice network centrality. Furthermore, this positive relationship was only significant for low self-monitoring individuals, but (...) not for high self-monitoring individuals. These findings indicated that individuals with high cognitive flexibility were more likely to have central positions in the advice network; however, this effect was attenuated as their self-monitoring increased. (shrink)

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In this article, we point out the problem on Stanley and Williamson’s reconstruction of Ryle’s regress argument to refute their critique toward Ryle’s argument. We identify the object of Ryle’s criticism through analysing some intellectualisms which try to solve the regress argument. To reconcile the contradiction of intellectualism and anti-intellectualism, we propose a moderate version of intellectualism – Intellectual Explanation Thesis – which does not lead to infinite regress while maintains the necessary connection between knowledge-how and knowledge-that. This alternative thesis (...) introduces a criterion for judging whether an action is intellectual or not, therefore offers a new perspective on the complex relationship between intellectual action and knowledge. (shrink)

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Many studies have shown that about three biological motions can be maintained in working memory. However, no study has yet analyzed the difficulties of experiment materials used, which partially affect the ecological validity of the experiment results. We use the perspective of system anatomy to decompose BM, and thoroughly explore the influencing factors of difficulties of BMs, including presentation duration, joints to execute motions, limbs to execute motions, type of articulation interference tasks, and number of joints and planes involved in (...) the BM. We apply the change detection paradigm supplemented by the articulation interference task to measure the BM working memory capacity of participants. Findings show the following: the shorter the presentation duration, the less participants remembered; the more their wrist moved, the less accurate their memory was; repeating verbs provided better results than did repeating numerals to suppress verbal encoding; the more complex the BM, the less participants remembered; and whether the action was executed by the handed limbs did not affect the WMC. These results indicate that there are many factors that can be used to adjust BM memory load. These factors can help sports psychology professionals to better evaluate the difficulty of BMs, and can also partially explain the differences in estimations of BM WMC in previous studies. (shrink)

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Contextual mobile advertising, with the advantages of high interactivity and immersive experience, is the mainstream trend of future Internet advertising. Current studies have explored the benefits of contextual mobile advertising while lacking the analysis of contextual mobile advertising factors on consumer purchase intentions. This study investigates the mechanisms by which the characteristics of contextual mobile advertising evoke consumers' purchase intentions through advertising attitudes to reveal how extroversion and neuroticism in personal traits moderate the relationship between characteristics of contextual mobile advertising (...) and advertising attitudes. Based on a sample of 543 community residents with mobile shopping experience in China, this study uses structural equation modeling to validate the relationships between the variables and draws conclusions. The findings help advertisers to grasp the important characteristics of contextual mobile advertising, improve consumers' attitudes toward advertising, and enhance purchase intentions. Furthermore, it is possible to expand perceptions of the effectiveness of contextual mobile advertising among consumers with different personality traits. (shrink)

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ObjectiveTwo subregions of the dorsolateral prefrontal cortex have been identified as effective repetitive transcranial magnetic stimulation targets for the “anxiosomatic” and “dysphoric” symptoms, respectively. We aimed to develop a convenient approach to locate these targets on the scalp.Materials and methodsIn a discovery experiment, the two personalized targets were precisely identified on 24 subjects using a neuronavigation system. Then, a localized approach was developed based on individual scalp landmarks. This “landmark-based approach” was replicated and validated in an independent cohort. Reliability of (...) the approach was tested by calculating the correlation of both the inter-rater and intra-rater results. Validity was tested by comparing the mean distance between the personalized and landmark-based targets to the TMS spatial resolution. We further conducted a total of 24 sham rTMS sessions to estimate the misplacement between the coil center and target during a 10-min stimulation without neuronavigation.ResultsThe parameters of the “landmark-based approach” in the discovery experiment were replicated well in an independent cohort. Using discovery parameters, we successfully identified the symptom-specific targets in the independent cohort. Specifically, the mean distance between the personalized and landmark-based targets on the cortex was not significantly larger than 5 mm. However, the personalized and landmark-based targets distance exceeded 5 mm in more than 50% of subjects. During the 10-min sham rTMS session, the average coil misplacement was significantly larger than 5 mm.ConclusionThe “landmark-based approach” can conveniently and reliably locate the two symptom-specific targets at group level. However, the accuracy was highly varied at individual level and further improvement is needed. (shrink)

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Adopting free word association test, the present study investigated the developmental features of Yi students’ Chinese mental lexicon. Eighty primary school students and 85 senior high school students in two typical Yi-Han bilingual schools in Yuexi County were recruited as the research subjects. With Yi language as their L1, all the participants started learning Chinese after entering primary school. The stimuli were 108 words selected from the 9,000 most frequently used words in modern Chinese, including 36 nouns, 36 verbs, and (...) 36 adjectives. The responses were analyzed using the framework proposed by the responses were analyzed using a three-layer framework. The results showed that the mental lexicon of Yi primary and middle school students were mainly connected with each other by meanings, with form-based connection followed. Compared with previous studies, their phonological associations accounted for a relatively higher proportion, while semantic associations were relatively weakened. Syntagmatic associations were of primary importance, with paradigmatic and encyclopedic associations followed. While syntagmatic relation was centered on determinative and governing associations, paradigmatic relation was dominated by adjacent and reverse associations. The development rate of syntagmatic associations was faster than that of paradigmatic and encyclopedic associations; no significant improvement was found on students’ adjacent, layer, homogeneous and supplement associations from primary school to high school. The findings generate important implications for Yi students’ Chinese language education. (shrink)

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This article aims to explore contemplative practitioners’ direct lived experiences of enlightenment and develop a multi-item scale measuring these experiences. A mixed-method approach was adopted. The first study is a phenomenological study that interviewed 24 participants with enlightenment experiences and the second study is a scale-development study that recruited 1130 participants for scale validation. Two major clusters of the enlightenment experiences including sensory feelings and nondual realizations emerged from the phenomenological study and the four main themes of the realizations were (...) formed: intrapersonal insights, inspiration internalization, interpersonal compassion, and interconnectedness. After exploratory factor analysis and confirmatory factor analysis, a three-factor structure of the Spiritual Enlightenment Experience Scale, including nonduality, sensory clarity, and sensory disappearance were revealed. The findings of the research depicted a fuller picture of spiritual enlightenment experiences which may contribute to the current knowledge of religiosity and spirituality, as well as transpersonal psychology. The results suggest that the core components of spiritual enlightenment experiences include sensory clarity and disappearance experiences and realizations of nonduality. (shrink)

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ObjectiveTo elucidate the clinical, radiologic characteristics of Leber’s hereditary optic neuropathy (LHON) associated with the other diseases.Materials and methodsClinical data were retrospectively collected from hospitalized patients with LHON associated with the other diseases at the Neuro-Ophthalmology Department at the Chinese People’s Liberation Army General Hospital (PLAGH) from December 2014 to October 2018.ResultsA total of 13 patients, 24 eyes (10 men and 3 women; mean age, 30.69 ± 12.76 years) with LHON mitochondrial DNA (mtDNA) mutations, were included in the cohort. 14502(5)11778(4)11778 (...) &11696(1)12811(1)11696(1)3460(1). One patient was positive for aquaporin-4 antibody (AQP4-Ab), and two were positive for myelin oligodendrocyte glycoprotein antibody (MOG-Ab). Three patients were associated with idiopathic optic neuritis (ON). Two patients were with compression optic neuropathy. Three patients were with the central nervous system (CNS) diseases. One patient was with proliferative diabetic retinopathy (PDR) and one with idiopathic orbital inflammatory syndrome (IOIS). At the onset, visual acuity (VA) in eighteen eyes was below 0.1, one eye was 0.5, five eyes were above 0.5, while VA in sixteen eyes was below a 0.1 outcome, three eyes experienced moderate vision loss. MRI images showed T2 lesions and enhancement in nine patients who received corticosteroids treatment; additional immune modulators treatment was performed on two patients. None of the patients had relapse during the follow-up time.ConclusionLeber’s hereditary optic neuropathy can be accompanied with multiple-related diseases, especially different subtypes of ON, which were also exhibited with IOIS and compression optic neuropathy for the first time in this cohort. This condition may be a distinct entity with an unusual clinical and therapeutic profile. (shrink)

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Experiential and behavioural aspects of emotions can be measured readily but developing a contactless measure of emotions’ physiological aspects has been a major challenge. We hypothesised that different emotion-evoking films can produce distinctive facial blood flow patterns that can serve as physiological signatures of discrete emotions. To test this hypothesis, we created a new Transdermal Optical Imaging system that uses a conventional video camera to capture facial blood flows in a contactless manner. Using this and deep machine learning, we analysed (...) videos of the faces of people as they viewed film clips that elicited joy, sadness, disgust, fear or a neutral state. We found that each of these elicited a distinct blood flow pattern in the facial epidermis, and that Transdermal Optical Imaging is an effective contactless and inexpensive tool to the reveal physiological correlates of discrete emotions. (shrink)

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With the rapid development of the Internet, political culture plays an increasingly prominent role in ethical guidance and value orientation, and the intergenerational inheritance of political culture in various countries needs to be carried out in a sophisticated way. From the perspective of empathic narrative, this study applies the network text analysis method to detect the cultural communication regularities to the contemporary young adults in online political communication and explores contemporary young adults’ perception of online political culture through empirical analysis. (...) Moreover, it proposes the empathic narrative logic of online political communication by comparing the existing communication elements and the urgently needed communication elements. Our findings suggest that we need to deepen the cognitive empathy, stabilize the emotional empathy, and adjust the emotional bias in online political communication. (shrink)

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In this study, we propose a way to link the scholarly contents of scientific papers by constructing a knowledge graph based on the semantic organization of argumentation units and relations in scientific papers. We carried out an argumentation graph data model aimed at linking multiple discourses, and also developed a semantic annotation platform for scientific papers and an argumentation graph visualization system. A construction experiment was performed using 12 articles. The final argumentation graph has 1,262 nodes and 1,628 edges, including (...) 1,628 intra-article relations and 190 inter-article relations. Knowledge evolution representation, strategic reading, and automatic abstracting use cases are presented to demonstrate the application of the argumentation graph. In contrast to existing knowledge graphs used in academic fields, the argumentation graph better supports the organization and representation of scientific paper content and can be used as data infrastructure in scientific knowledge retrieval, reorganization, reasoning, and evolution. Moreover, it supports automatic abstract and strategic reading. (shrink)

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As global warming becomes increasingly prominent, countries worldwide advocate for a low-carbon economy to cope with the pressure to reduce greenhouse gas emissions. The Chinese government has proposed a “dual carbon” goal of peaking carbon emissions by 2030 and becoming carbon neutral by 2060. The disclosure of carbon information by Chinese enterprises has attracted widespread attention from society. This study selects the constituents of the Social Responsibility Index of China Shanghai Stock Exchange from 2016 to 2020 as samples to empirically (...) analyze the relationship between the level of carbon information disclosure and corporate value, and the moderating effect of greenwashing behavior. Results indicated that the quality of carbon disclosure is positively correlated with the enterprise value. Greenwashing behavior promotes the positive impact of carbon disclosure quality on enterprise value in the short run, but this promoting effect fades in the long run. We further found that the carbon information disclosure of non-heavy-pollution enterprises has a more obvious positive impact on enterprise value than that of heavily polluting enterprises. Additionally, the positive impact of carbon information disclosure on enterprise value is more visible among enterprises in a good legal environment than those in a poor legal environment. This study enriches the relevant literature on carbon information disclosure and enterprise “greenwashing” behavior and has practical significance for promoting China’s low-carbon development in the context of ecological civilization and improving the enthusiasm for the quality of enterprise carbon information disclosure. (shrink)

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Partitioner algebras are defined by Baumgartner and Weese 619) as a natural tool for studying the properties of maximal almost disjoint families of subsets of ω. We prove from PFA+ and that there exists a partitioner algebra which contains a subalgebra which is not representable as a partitioner algebra.

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The Shanghai Cooperation Organization is the largest regional security and cooperation organization and has existed for nearly two decades. Since its inception China and Russia have acted as the driving force behind it, playing a leading role in its development. The main goals of the Big Two’s cooperation are to ensure the Eurasian corridor is developed, to promote collective security through regional cooperation organizations, including the United Nations, and to recast the world order on the basis of political dialogue, mutual (...) respect, equality and international law. Nonetheless there are disagreements between China and Russia and among the member states. China is keen to tackle terrorism, extremism and separatism and pursue economic collaboration, while Russia is more ambitious about transforming the SCO into a strategic counterbalance to U.S. hegemony. Looking forward, people may wonder how China, a rising economic power and military force, will continue to share liabilities within the SCO, particularly with Russia but also the other member states. There is no definite answer to that question at the moment. All we can say is that the SCO has steadily evolved into one of the most multilateral and dynamic organizations. But if progress is to be sustained, reforms and systematic change are necessary. (shrink)

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Contemporary social reform promotes rapid social transformation, and social practice has a special educational function in higher education. However, research shows weak willingness to participate in social practice among college students. Using the mixed research method, 438 completed questionnaire surveys on perceived environmental support were collected from college students. The influence of perceived environmental support on Chinese college students’ willingness to participate in social practice was analyzed using partial least squares structural equation modeling, and an empirical test was conducted. The (...) findings are as follows: Perceived environmental support significantly impacts students’ participation attitude and perceived behavioral control. Participation attitude and perceived behavioral control significantly influence participation intention, but behavioral norms have no significant influence. Participation intention and perceived behavioral control significantly influence actual behavior. This study provides the theoretical basis of perceived environmental support for future research on social practice participation intention and offers some theoretical guidance for the implementation of social practice in China. (shrink)

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Governments have adopted strict home quarantine measures during the COVID-19 pandemic. A monotonous, barren, and under-stimulating environment can cause state boredom, and people often deal with boredom via novelty-seeking behavior. Novelty-seeking behavior can be divided into “novelty input” and “novelty output.” The former refers to obtaining novel information such as browsing the Web; the latter refers to engaging in creative behavior such as literary creation. This study explores the relationship between two types of novelty-seeking behavior and individual state boredom during (...) home quarantine, along with the moderation effect of trait creativity. The study sample consists of 582 Chinese college students who were quarantined at home during the COVID-19 pandemic. Participants completed the Multidimensional State Boredom Scale, the Williams Creativity Aptitude Test, and self-compiled questionnaires of novelty input and novelty output. The results show that there is no significant relationship between novelty input or novelty output and boredom during the COVID-19 quarantine. Trait creativity is found to negatively moderate the relationship between the two means of novelty seeking and boredom. Specifically, novelty output negatively predicts the state boredom of individuals with high creativity, while novelty input positively predicts the state boredom of individuals with low creativity. Our findings suggest that different novelty-seeking behaviors may have different effects on the boredom level of individuals with high versus low creativity during quarantine. During a quarantine period, individuals should avoid excessively engaging in novelty input behaviors aimed at escaping boring situations. (shrink)

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In modern day technology, the level of knowledge is increasing day by day. This increase is in terms of volume, velocity, and variety. Understanding of such knowledge is a dire need of an individual to extract meaningful insight from it. With the advancement in computer and image-based technologies, visualization becomes one of the most significant platforms to extract, interpret, and communicate information. In data modelling, visualization is the process of extracting knowledge to reveal the detail data structure and process of (...) data. The proposed study aim is to know about the user knowledge, data modelling, and visualization by handling through the fuzzy logic-based approach. The experimental setup is validated through the data user modelling dataset available in the UCI web repository. The results show that the model is effective and efficient in situations where uncertainty and complexity arise. (shrink)

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Innovative loss‐of‐function techniques developed in recent years have made it much easier to target specific genomic loci at transcriptional levels. CRISPR interference (CRISPRi) has been proven to be the most effective and specific tool to knock down any gene of interest in mammalian cells. The catalytically deactivated Cas9 (dCas9) can be fused with transcription repressors to downregulate gene expression specified by sgRNA complementary to target genomic sequence. Although CRISPRi has huge potential for gene knockdown, there is still a lack of (...) systematic guidelines for efficient and widespread use. Here we describe the working mechanism and development of CRISPRi, designing principles of sgRNA, delivery methods and applications in mammalian cells in detail. Finally, we propose possible solutions and future directions with regard to current challenges. (shrink)

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Although China’s wind industry has made great progress in recent years, the wind abandonment phenomenon caused by the unbalanced development of regional wind power is still prominent. It is particularly important for the scientific development of wind power to accurately measure the utilization efficiency of wind power and understand its regional differences in China. This study establishes the improved super-efficiency slack-based measure model and long short-term memory network models, systematically and comprehensively measures and predicts the wind power utilization efficiency of (...) 30 regions in China from 2013 to 2020, and explores regional differences in wind power utilization efficiency. Our results show the following: China’s overall wind power utilization efficiency is relatively low but has been on a steady upward trend since 2013. Regional differences are obvious, showing that the spatial distribution pattern of wind power utilization efficiency is greatest in Northeast China, followed by North China, East China, South China, Northwest China, and Central China. The “Three-North” region with abundant wind energy resources has relatively high wind power utilization efficiency and exhibits a good development trend. East China, South China, and Central China, where wind energy resources are relatively poor, have low wind power utilization efficiency, and their development trends are not stable and are more prone to change. The utilization efficiency of wind power in coastal areas is generally better than that in inland areas. There are also differences among the thirty Chinese regions studied. Inner Mongolia and Shandong have achieved real efficiency in wind power utilization efficiency, with optimal allocation of input and output, and a good development trend. The other 28 regions have varying degrees of inefficiency, and there is still room for improvement. (shrink)

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This article reports on a study that took a Dynamic Systems Theory perspective to second language motivational self system. More specifically, it investigated the influence of an Intensive English Reading course based on the Production-Oriented Approach upon the L2MSS of Chinese university English majorsfrom the DST perspective. To this end, two intact classes composed of 50 students were assigned into experimental group and control group, who responded to an L2MSS scale before and after the one-semester intervention. Eight and five students (...) were respectively selected using the purposive sampling method from the experimental and control groups for follow-up semi-structured interviews. The quantitative results revealed that the overall and dimensional levels of L2MSS were significantly strengthened over time in the EG while kept stable in the CG. The qualitative results suggested that the enhanced Ideal L2 Self of the participants stemmed from an attractor basin that was deepened by a number of attractors encompassing Output Tasks and Peer Performance. The interview results also showed that the increased L2 Learning Experience of the participants pertained to an attractor basin that was consolidated by an array of attractors containing Output Tasks, Teacher Guidance, Group Discussion, and Peer Assessment. The findings indicated that the attractors at the subjective and social dimensions in the POA-based course collectively worked together to cause changes in L2MSS among the participants. The implications for intervening L2 motivation from a POA approach in English as a Foreign Language classrooms were discussed. (shrink)

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