WO2024223526A3 - Cell culture membrane insert, and device and method for detecting at least one electrochemical variable of a cell culture - Google Patents

Abstract

The invention relates to a cell culture membrane insert (120) and to a device (110) and a method (210) for detecting at least one electrochemical variable of a cell culture (112). The cell culture (112) can be at least one of any cell type of at least one species in a mono- or co-culture, particularly preferable are cell cultures which are exposed to at least one gas or aerosol. The cell culture membrane insert (120) comprises - an open support (128) which is designed to expose a cell culture (112) to at least one gas or aerosol (114), wherein a membrane (130) which has at least one surface (132) is introduced into the support (128), said surface being designed to receive the cell culture (112), and the membrane (130) has o pores (134) for diffusing a nutrient medium (136) through the membrane (130) and o an electrode assembly (130) on the at least one surface (132) designed to receive the cell culture (112), said electrode assembly (130) comprising at least two electrodes, and - at least two contact points (146, 146'). Each contact point (146, 146') is designed to produce an electric connection between one of the electrodes and a respective electric contact (144, 144') which is not encompassed by the cell culture membrane insert (120). In particular, the method and the device (110) allow a time-resolved, non-invasive, and marking-free examination and monitoring of cell cultures (112) which can be exposed to at least one gas or aerosol (114) in particular, said examination and monitoring being as close to reality as possible.