Movatterモバイル変換

[0]ホーム
URL:

WO2016193846A3 - Degenerate primer sets - Google Patents

Degenerate primer setsDownload PDF

Info

Publication number
WO2016193846A3
WO2016193846A3PCT/IB2016/052852IB2016052852WWO2016193846A3WO 2016193846 A3WO2016193846 A3WO 2016193846A3IB 2016052852 WIB2016052852 WIB 2016052852WWO 2016193846 A3WO2016193846 A3WO 2016193846A3
Authority
WO
WIPO (PCT)
Prior art keywords
primers
primer sets
degenerate primer
design
enough
Prior art date
Application number
PCT/IB2016/052852
Other languages
French (fr)
Other versions
WO2016193846A2 (en
Inventor
Sonia CHOTHANI
Sitharthan Kamalakaran
Original Assignee
Koninklijke Philips N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips N.V.filedCriticalKoninklijke Philips N.V.
Publication of WO2016193846A2publicationCriticalpatent/WO2016193846A2/en
Publication of WO2016193846A3publicationCriticalpatent/WO2016193846A3/en
Anticipated expirationlegal-statusCritical
Ceasedlegal-statusCriticalCurrent

Links

Classifications

Landscapes

Abstract

A set of broad ranging primers to allow rapid amplification of any pathogenic bacteria as well as a methodology for the design of these primers. One approach to providing such a set in accord with the present invention is to design primers for regions/genes that are conserved enough for universal primer binding and at the same time have enough variability to give distinguishing capacity. The set may be selected based on optimizing for degeneracy and coverage.
PCT/IB2016/0528522015-05-292016-05-17Degenerate primer setsCeasedWO2016193846A2 (en)

Applications Claiming Priority (2)

Application NumberPriority DateFiling DateTitle
US201562167996P2015-05-292015-05-29
US62/167,9962015-05-29

Publications (2)

Publication NumberPublication Date
WO2016193846A2 WO2016193846A2 (en)2016-12-08
WO2016193846A3true WO2016193846A3 (en)2017-02-02

Family

ID=56119712

Family Applications (1)

Application NumberTitlePriority DateFiling Date
PCT/IB2016/052852CeasedWO2016193846A2 (en)2015-05-292016-05-17Degenerate primer sets

Country Status (1)

CountryLink
WO (1)WO2016193846A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
KR101889146B1 (en)*2016-06-282018-08-17재단법인대구경북과학기술원Method for rapid design of valid high-quality primers and probes for multiple target genes in qPCR experiments
KR101912555B1 (en)*2018-01-302018-10-26재단법인대구경북과학기술원Method for rapid design of valid high-quality primers and probes for multiple target genes in qPCR experiments

Citations (15)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US6054278A (en)*1997-05-052000-04-25The Perkin-Elmer CorporationRibosomal RNA gene polymorphism based microorganism identification
US20060115873A1 (en)*2004-12-012006-06-01Peak Sang-HyunNovel exothermic material, method and device for lysing cells using the exothermic material
CN1824805A (en)*2005-12-092006-08-30上海大学 Gene Chip Detection Method of Bacteria in Sewage
US20060211002A1 (en)*2005-03-182006-09-21Jan WeileAntibiotic susceptibility and virulence factor detection in Pseudomonas aeruginosa
US20060246473A1 (en)*2004-12-232006-11-02Oh Ji-YoungPrimer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species
EP1801238A1 (en)*2005-12-202007-06-27Samsung Electronics Co., Ltd.Microarray substrate, method of manufacturing the same, microarray using the microarray substrate, and method of analyzing target biomolecule using the microarray
US20080076127A1 (en)*2006-09-262008-03-27Samsung Electronics Co., Ltd.Method and compositions for detecting helicobacter pylori
CN101210262A (en)*2007-12-242008-07-02哈尔滨工业大学 A Method for Analyzing the Structural Composition of Microbial Communities Using Single-Strand Conformational Polymorphism
WO2009049007A2 (en)*2007-10-102009-04-16Magellan Biosciences, Inc.Compositions, methods and systems for rapid identification of pathogenic nucleic acids
WO2009124085A1 (en)*2008-04-012009-10-08University Of WashingtonImprovement of sequence data by reduction of noise due to carry-over primer
US20090263809A1 (en)*2008-03-202009-10-22Zygem Corporation LimitedMethods for Identification of Bioagents
WO2012116289A2 (en)*2011-02-252012-08-30Tricorder Diagnostics, LlcMicrobial signatures as indicators of radiation exposure
US20120231459A1 (en)*2011-03-092012-09-13Gen-Probe IncorporatedChemiluminescent probes for multiplex molecular quantification and uses thereof
US20130052172A1 (en)*2011-08-262013-02-28John Edward BakerMethods for diagnosing and treating cardiac defects
US20130315922A1 (en)*2009-04-272013-11-28Gerard EberlMethod for inducing lymphoid tissue and modulating intestinal homeostasis

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US6054278A (en)*1997-05-052000-04-25The Perkin-Elmer CorporationRibosomal RNA gene polymorphism based microorganism identification
US20060115873A1 (en)*2004-12-012006-06-01Peak Sang-HyunNovel exothermic material, method and device for lysing cells using the exothermic material
US20060246473A1 (en)*2004-12-232006-11-02Oh Ji-YoungPrimer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species
US20060211002A1 (en)*2005-03-182006-09-21Jan WeileAntibiotic susceptibility and virulence factor detection in Pseudomonas aeruginosa
CN1824805A (en)*2005-12-092006-08-30上海大学 Gene Chip Detection Method of Bacteria in Sewage
EP1801238A1 (en)*2005-12-202007-06-27Samsung Electronics Co., Ltd.Microarray substrate, method of manufacturing the same, microarray using the microarray substrate, and method of analyzing target biomolecule using the microarray
US20080076127A1 (en)*2006-09-262008-03-27Samsung Electronics Co., Ltd.Method and compositions for detecting helicobacter pylori
WO2009049007A2 (en)*2007-10-102009-04-16Magellan Biosciences, Inc.Compositions, methods and systems for rapid identification of pathogenic nucleic acids
CN101210262A (en)*2007-12-242008-07-02哈尔滨工业大学 A Method for Analyzing the Structural Composition of Microbial Communities Using Single-Strand Conformational Polymorphism
US20090263809A1 (en)*2008-03-202009-10-22Zygem Corporation LimitedMethods for Identification of Bioagents
WO2009124085A1 (en)*2008-04-012009-10-08University Of WashingtonImprovement of sequence data by reduction of noise due to carry-over primer
US20130315922A1 (en)*2009-04-272013-11-28Gerard EberlMethod for inducing lymphoid tissue and modulating intestinal homeostasis
WO2012116289A2 (en)*2011-02-252012-08-30Tricorder Diagnostics, LlcMicrobial signatures as indicators of radiation exposure
US20120231459A1 (en)*2011-03-092012-09-13Gen-Probe IncorporatedChemiluminescent probes for multiplex molecular quantification and uses thereof
US20130052172A1 (en)*2011-08-262013-02-28John Edward BakerMethods for diagnosing and treating cardiac defects

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 24 January 2008 (2008-01-24), "Bacterial gene detecting probe #2.", XP002761972, retrieved from EBI accession no. GSN:AOD61978 Database accession no. AOD61978*

Also Published As

Publication numberPublication date
WO2016193846A2 (en)2016-12-08

Similar Documents

PublicationPublication DateTitle
IL290678B1 (en)Novel crispr enzymes and systems
EP3286338A4 (en)Amplification with primers of limited nucleotide composition
IL254044A0 (en)3-substituted 1,3,4-oxadiazole and thiadiazole compounds as immunomodulators
EP3544100A4 (en)Primary battery and moisture sensor
WO2016170348A3 (en)Sarna compositions and methods of use
WO2016134335A3 (en)Pvrig polypeptides and methods of treatment
EP3137843A4 (en)Projectile with enhanced ballistics
EP3109254A4 (en)Crosslinked nucleoside and nucleotide
EP3291360A4 (en)Battery pack and vehicle including such battery pack
EP3270728A4 (en)Anti-sprain and shock-absorbing balance sole and footgear
IL256322A (en)Improved formulations of deferasirox and methods of making the same
PL3433566T3 (en)Propellant charge or grain
Montes et al.Stochastic dominance with imprecise information
SG11201708760PA (en)Nucleic acid-cationic polymer compositions and methods of making and using the same
SG11201900459RA (en)Mg53 mutants, methods of making the same, and uses thereof
EP3218503A4 (en)Vectors and methods for targeted integration in loci comprising constitutively expressed genes
EP3354155A4 (en) HOUSING OR BAG FACILITATING THE CHANGE OF LOAD CABLE
HK1256159A1 (en)Immuno-oncology mesodermal progenitor (iomp) cell
EP3643789A4 (en)Pcr primer pair and application thereof
GB201516975D0 (en)PARK2 as epigenetic marker for the identification of immune cells, in particular monocytes
WO2016193846A3 (en)Degenerate primer sets
EP3097195A4 (en)Zea mays regulatory elements and uses thereof
EP3643787A4 (en)Pcr primer pair and application thereof
HUE057044T2 (en) Preparations specifically targeting cathepsin C and / or CELA1 and / or CELA3A and / or structurally related enzymes for the treatment and / or prevention of cell or tissue necrosis
MX366113B (en)Diagnostic methods and compositions.

Legal Events

DateCodeTitleDescription
121Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number:16728734

Country of ref document:EP

Kind code of ref document:A2

NENPNon-entry into the national phase

Ref country code:DE

122Ep: pct application non-entry in european phase

Ref document number:16728734

Country of ref document:EP

Kind code of ref document:A2
[8]ページ先頭
©2009-2025 Movatter.jp