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WO2009060012A2 - Plant health compositions comprising a beneficial microorganism and a pesticide - Google Patents

Plant health compositions comprising a beneficial microorganism and a pesticide
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Publication number
WO2009060012A2
WO2009060012A2PCT/EP2008/065028EP2008065028WWO2009060012A2WO 2009060012 A2WO2009060012 A2WO 2009060012A2EP 2008065028 WEP2008065028 WEP 2008065028WWO 2009060012 A2WO2009060012 A2WO 2009060012A2
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ustilago
plant
tilletia
plant health
pyrenophora
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PCT/EP2008/065028
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French (fr)
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WO2009060012A3 (en
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Dirk Voeste
Lutz Brahm
Mathijs Wuts
Egon Haden
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Basf Se
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Abstract

The present invention relates to plant health compositions comprising, as active components, at least one viable microorganism having plant health activity and at least one chemical compound having plant health and/or crop protection activity; methods for controlling phytopathogenic harmful pests and method for improving plant health by applying such plant health compositions; plant health formulations prepared from said compositions; kits comprising said plant health compositions as well as plant seeds treated with such compositions or formulations.

Description

Plant health compositions comprising a beneficial microorganism and a pesticide
Description
The present invention relates to plant health compositions comprising, as active components, at least one viable microorganism having plant health activity and at least one chemical compound having plant health and/or crop protection activity; methods for controlling phytopathogenic harmful pests and methods for improving plant health by applying such plant health compositions; plant health formulations prepared from said compositions; kits comprising said plant health compositions as well as plant seeds treated with such compositions or formulations.
Background of the invention
It is well known in the art to treat plants or propagules thereof with benificial microorganisms or pesticides in order to improve the plant health by protecting the plants from disease or by improving nutrition and/or growth of the plants.
In the field of plant health and plant protection, there is a continuous need to develop new combinations of active ingredients and new application technologies, which allow an improved treatment of plants and have beneficial effects on the plant health.
The present inventors were looking for new solutions of the above problem.
Summary of the invention
In order to solve the above problem the present inventors developed plant health composition, comprising the following components: 1 ) at least one viable microorganism (I) having plant health activity, and 2) at least one chemical compound (II) having plant health activity and/or crop protection activity.
Particular compositions of the components 1 ) and 2) allow a more significant improvement of plant health than is possible with the microbial strains (I) on the one hand and with the individual compounds (II) on the other hand alone (synergistic combinations).
Component 1 ) embraces not only the isolated, pure cultures of a microbial strain, but also their suspensions in a whole broth culture or a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the strain.
Detailed description of the invention
a) General definitions
"Whole broth culture" refers to a liquid culture containing both cells and media.
"Supernatant" refers to the liquid broth remaining when cells grown in broth are removed by centrifugation, filtration, sedimentation, or other means well known in the art.
The term "metabolite" refers to any compound, substance or byproduct of a fermentation or a microorganism that has plant health activity.
"Propagules" of a plant are all types of plant propagation material. The term embraces seeds, grains, fruit, tubers, rhizomes, spores, cuttings, offshoots, meristem tissues, single and multiple plant cells and any other plant tissue from which a complete plant can be obtained. One particular propagule is seed.
"Locus" means soil, area, material or environment where the plant is growing or intended to grow.
"Plant" embraces individual plants or plant varieties of any type of plants, in particular agricultural, silvicultural and ornamental plant.
"Pests" comprise in particular herbs, fungi, nematodes, molluscs, bacteria, viruses or insects harmful to a plant.
"Pesticides" comprise herbicides, fungicides, nematicides, molluscicides, bactericides and insecticides.
"Plant health" is intended to mean a condition of the plant, which is determined by several aspects alone or in combination with each other. One indicator for the condition of the plant is the "crop yield ". "Crop" and "fruit" are to be understood as any plant product which is further utilized after harvesting, e.g. fruits in the proper sense, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants), flowers (e.g. in the case of gardening plants, ornamentals) etc., that is anything of economic value that is produced by the plant. Another indicator for the condition of the plant is the "plant vig- our". The plant vigour becomes manifest in several aspects, too, some of which are visual appearance, e.g. leaf colour, fruit colour and aspect, amount of dead basal leaves and/or extent of leaf blades, plant weight, plant height, extent of plant verse (lodging), number, strongness and productivity of tillers, panicles' length, extent of root system, strongness of roots, extent of nodulation, in particular of rhizobial nodulation, point of time of germination, emergence, flowering, grain maturity and/or senescence, protein content, sugar content and the like. Another indicator for the condition of the plant is the plant's "tolerance or resistance to biotic and abiotic stress factors".
"Crop protection" is intended to mean a control effect, as for example the prevention, reduction or elimination of at least one crop disease, fungus or other pest. A chemical compound having crop protection activity thus may prevent the onset and/or spread of at least one fungus or other pest at, on or in at least one crop, which otherwise might cause serious damage to the crop resulting, for example, in a loss of yield.
A substance or microorganism having "plant health activity" has a beneficial, advantageous effect on at least one of the above indicators.
"Biotic and abiotic stress", especially over longer terms, can have harmful effects on plants. Biotic stress is caused by living organisms, such as pests (e.g. insects, arach- nides, nematodes etc.), competing plants (for example weeds), phythopathogenic fungi and other microorganisms such as bacteria and viruses. Abiotic stress is caused for example by extremes in temperature such as heat or cold or strong variations in temperature or temperatures unusual for the specific season, drought, extreme wetness, high salinity, radiation (e.g. increased UV radiation due to the decreasing ozone protec- tive layer), increased ozone levels and organic pollution (e.g. by phythotoxic amounts of pesticides) or inorganic pollution (e.g. by heavy metal contaminants). As a result, the quantity and the quality of the stressed plants, their crops and fruits decrease. As far as quality is concerned, reproductive development is usually severely affected with consequences on the crops, which are important for fruits or seeds. Synthesis, accumula- tion and storage of proteins are mostly affected by temperature; growth is slowed by almost all stresses; polysaccharide synthesis, both structural and storage, is reduced or modified: these effects bring to a decrease in biomass and to changes in the nutritional value of the product.
Of course, the three above indicators for the health condition of a plant may be interdependent and may result from each other. For example, an increased resistance to biotic and/or abiotic stress may lead to a better plant vigour, e.g. to better and bigger crops, and thus to an increased yield. Inversely, a more developed root system may result in an increased resistance to biotic and/or abiotic stress. However, these inter- dependencies and interactions are neither all known nor fully understood and therefore the different indicators will be described separately.
According to the present invention, "increased yield" of a plant, in particular of an agri- cultural, silvicultural and/or ornamental plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the composition of the invention. According to the present invention, it is preferred that the yield be increased by at least 0,5 %, more preferred at least 1 %, even more preferred at least 2 %, still more preferred at least 4 %.
According to the present invention, "improved plant vigour" means that certain crop characteristics are increased or improved by a measurable or noticeable amount over the same factor of the plant produced under the same conditions, but without the appli- cation of the composition of the present invention.
"Improved plant vigour" can be characterized, among others, by following improved properties of the plant:
(a) improved vitality of the plant, (b) improved quality of the plant and/or of the plant products, e.g.
(b.1 ) enhanced protein content,
(c) improved visual appearance,
(d) delay of senescence,
(e) enhanced root growth and/or more developed root system, (f) enhanced nodulation, in particular rhizobial nodulation,
(g) longer panicles,
(h) bigger leaf blade,
(i) less dead basal leaves,
(j) increased or improved plant stand density, (k) less plant verse (lodging),
(1) increased plant weight,
(m) increased plant height,
(n) tillering increase,
(o) stronger and/or more productive tillers, (p) less non-productive tillers,
(q) enhanced photosynthetic activity and/or enhanced pigment content and thus (q.1 ) greener leaf color,
(r) earlier and improved germination,
(s) improved emergence, (t) increased shoot growth, (u) earlier flowering, (v) esrlier fruiting, (w) earlier grain maturity, (x) less fertilizers needed, (y) less seeds needed.
The improvement of the plant vigor according to the present invention particularly means that the improvement of any one or several or all of the above mentioned plant characteristics are improved independently of the pesticidal action of the composition or active ingredients.
According to the present invention, "enhanced plant's tolerance or resistance to biotic and/or abiotic stress factors" means (1 ) that certain negative factors caused by biotic and/or abiotic stress are diminished in a measurable or noticeable amount as com- pared to plants exposed to the same conditions, but without being treated with the composition of the invention and/or (2) that the negative effects are not diminished by a direct action of the composition on the stress factors, e.g. by its fungicidal or insecti- cidal action which directly destroys the microorganisms or pests, but rather by a stimulation of the plants' own defensive reactions against said stress factors.
Negative factors caused by biotic stress such as pathogens and pests are widely known and range from dotted leaves to total destruction of the plant.
Negative factors caused by abiotic stress are also well-known and can often be ob- served as reduced plant vigour (see above), e.g. dotted leaves, "burned leaves", reduced growth, less flowers, less biomass, less crop yields, reduced nutritional value of the crops, later crop maturity, to give just a few examples.
According to the invention, the tolerance of and/or resistance against biotic stress fac- tors may be enhanced. Thus, the inventive compositions may be used for stimulating the natural defensive reactions of a plant against a pathogen and/or a pest. Thereby, the plant can be protected against unwanted microorganisms such as phytopathogenic fungi, bacteria or viruses and/or against pests such as insects, arachnids and nematodes, and it has been found that the inventive compositions result in plant strengthen- ing effects. Therefore, they are useful for mobilizing the plant's defense mechanisms against the attack of unwanted microorganisms and/or pests. Consequently, the plant becomes tolerant or even resistant towards these microorganisms and/or pests. Unwanted microorganisms in this context are phytopathogenic fungi and/or bacteria and/or viruses. Unwanted pests are insects and/or arachnids and/or nematodes. The inventive compositions may be used for stimulating the natural defensive reactions of a plant against microorganisms as for example against phytopathogenic fungi, bacteria and/or viruses, wherein the treated plant may develop increased defense mechanism against one of these pathogens or against two, three or all of these pathogens.
According to the invention also the tolerance of and/or resistance against abiotic stress factors may be enhanced. Thus, the inventive compositions may be used for stimulating a plant's own defensive reactions against abiotic stress such as extremes in temperature, e.g. heat or cold or strong variations in temperature or temperatures unusual for the specific season, drought, extreme wetness, high salinity, radiation (e.g. in- creased UV radiation due to the decreasing ozone protective layer), increased ozone levels, organic pollution (e.g. by phythotoxic amounts of pesticides) and/or inorganic pollution (e.g. by heavy metal contaminants).
"Particulate" comprises material present in the form of particles of any, regular or ir- regular shape and size, which particles may be individual particles or aggregates or agglomerates or compressed forms of such individual particles.
The parameter "aw" designates the water activity (correlating with the water content of a sample) The measurement of water activity may be performed with a Hygroscope DT instrument from Rotronic AG, Zurich, Switzerland. The product is placed in the sample holder and this is positioned in the measuring chamber thermostatted to 250C. After closing the measuring chamber and an equilibration time of 20 minutes, the instrument measurement value is read off.
"Cell counts" may be determined in the conventional manner by serial dilution with sterile 0.9% strength NaCI solution and subsequent plating on MRS agar (Difco Laboratories). "Colony-forming units" (cfu) may be counted after incubation for 48 hours at 370C. Only plates, which contained between 30 and at most 300 colonies were evaluated. Generally, 3 plates per stage were evaluated and the mean taken.
"Viability" of a microorganism describes the ability of the microorganism to grow and/or to multiply and/ or to differentiate.
The term "seed" embraces seeds and plant propagules of all kinds including but not limited to true seeds, seed pieces, suckers, corms, bulbs, fruit, tubers, grains, cuttings, cut shoots and the like and means in a preferred embodiment true seeds.
The term "seed treatment" comprises all suitable seed treatment techniques known in the art, such as seed dressing, seed coating, seed dusting, seed soaking, seed im- pregnation and seed pelleting. The term "coated with and/or containing" generally signifies that the active ingredient is for the most part on the surface of the propagation product at the time of application, although a greater or lesser part of the ingredient may penetrate into the propagation product, depending on the method of application. When the said propagation product is (re)planted, it may absorb the active ingredient.
By "simultaneous" application is understood the joint or separate application of components 1 ) and 2).
b) Preferred embodiments of the invention
A first aspect of the invention relates to a plant health composition, comprising the fol- lowing components 1 ) and 2):
1 ) comprising at least one viable or non-viable microorganism (I) having plant health activity, and/or crop protection activity
2) comprising at least one chemical compound (II) having plant health activity and/or crop protection activity.
In particular, said viable or non-viable beneficial microorganism (I) is a plant protective or plant performance or nutrition-improving microorganism (I).
In particular the plant health composition of the invention contain as component 1 ) a particulate formulation of at least beneficial microorganism (I) and a particulate carrier substance. Said carrier may be substantially composed of water-soluble or water- insoluble material or mixtures thereof. The microorganism cells may be embedded into the carrier material and/or may be adsorbed to the surface of the carrier material.
The carrier substance, which may added as coformulant prior to the drying to a suspension of usually freshly grown of microorganism cells, may be selected from mono-, oligo- and polysaccharides, polyols, polyethers, polymers, such as CMC or PVP, oligo- and polypeptides, from natural sources, such as milk, meat or cereals, derived substances or mixed substances, such as sweet whey powder, wheat semolina bran, pep- tone, alginates, mineral compounds, or mixtures of such materials. Said material may be dissolved in said suspension of microorganism cells, which mxture may then be dried in order to obtain particulate material. In another embodiment, said carrier may comprise a water insoluble, water-absorbent carrier substance, which may be is selected from any organic or inorganic material capable of removing moisture gently from the suspension of viable microorganisms, and in particular from the group consisting of zeolite, porous beads or powders, silica, ground agricultural products (as for example corn cobs), porous wood products, cellulose, cyclodextrins, and combinations thereof. The carrier may be admixed with a suspension of usually freshly grown of microorganism cells in order to form particulate material, which optionally my be further subjected to drying.
In addition, additives having a stabilizing action on the microorganism can be added to the mixture, preferably prior to the preparation of the particulate formulation, as for example antioxidants, such as alpha-tocopherol or ascorbic acid, or mixtures thereof. Furthermore, a stabilizing action can be exerted by other substances, which are selected from inorganic salts, such as alkali metal chlorides or alkaline earth metal chlo- rides, inorganic or organic buffers, such as alkali metal phosphate buffer, amino acids, such as aspartic acid or glutamic acid and the salts thereof, organic carboxylic acids, such as citric acid, organic nonvolatile solvents, such as DMSO, and other compounds, such as β-carotene and mixtures of these.
In a specific embodiment the particulate formulation comprises said carrier substance, as for example said water insoluble, water-absorbent carrier substance, wherein said carrier substance is present in an amount of at least about 40 %, as for example at lease 50, 60, 70, 80, 85, 90, 95, 96, 97, 98 or 99 % by the total weight of the formulation and at least one microorganism (I) mixed with said carrier.
According to a further embodiment said particulate formulation is coated in a manner known per se with a suitable compatible coating or encapsulating material.
Suitable encapsulating materials include, without limitation, native or modified chito- sans, native of modified starches, glucans or dextrins, celluloses modified so they are soluble, and any of a number of native or modified vegetable or microbial gums, including agars, guar, locust, carrageenan, xanthans, pectins, and the like, and combinations thereof.
Further suitable coating materials are polymers such as, for example, PVP, in particular a PVP product, which is commercially available under the trade name Kollidon VA64. Another usable coating system comprises a mixture of shellac and Kollidon 25 or 30, which may be supplemented with titanium dioxide and tallow. In particular the beneficial microorganism (I) is selected from the group consisting of bacteria, oomycetes, fungi, in any developmental stage thereof, and combinations of at least two said microorganisms from the same or different group or developmental stage. Examples of suitable microorganisms are given below in a subsequent section.
In the plant health composition of the invention said chemical compound (II) is selected from at least one pesticidal compounds, in particular fungicidal compounds of the subgroups A.1 to A.15 and/or at least parasiticidal compounds of the subgroups B1 to B.6. Combinations of two or more compounds of the same or different subgroup may also be used.
Specific examples of pesticidal compounds are given below in subsequent sections.
According to a further embodiment of the plant health composition according to the invention the components 1 ) and 2) are present in a weight ratio of from 1000:1 to 1 :1000, as for example 200:1 to 1 :200, 100:1 to 1 :100, as for example 90:1 to 1 :90, 80:1 to 1 :80, 75:1 to 1 :75, 50:1 to 1 :50, 25:1 to 1 :25 or 10:1 to 1 :10.
A further aspect of the invention relates to a method for controlling phytopathogenic harmful pests, wherein the pests, their habitat or the plants to be protected against pest attack, the locus where the plant is growing or is intended to grow and/or the plant propagules are/is treated with an effective amount of a component 1 ) and a component 2) as herein defined.
A further aspect of the invention relates to a method for improving plant health, in particular plant performance or nutrition, wherein the plants, the locus where the plant is growing or is intended to grow and/or the plant propagules are/is treated with an effective amount of a component 1 ) and a component 2) as herein defined.
According to these methods said components 1 ) and 2) are applied simultaneously, that is jointly or separately, or in succession.
A further aspect of the invention relates to the use of components 1 ) and 2) as herein defined for preparing a plant health agent (or formulation).
A further aspect of the invention relates to the use of components 1 ) and 2) as herein defined for treating transgenic plants or the seed thereof.
The plants to be treated in the method or use of the invention are selected from agricul- tural plants, silvicultural plants and ornamental plants. The agricultural plants are preferably selected from soybean, corn, wheat, triticale, barley, oats, rye, rape, millet, rice, sunflower, cotton, sugar beets, pome fruit, stone fruit, citrus, bananas, strawberries, blueberries, almonds, grapes, mango, papaya, peanuts, potatoes, tomatoes, peppers, cucurbits, cucumbers, melons, watermelons, garlic, onions, carrots, cabbage, beans, peas, lentils, alfalfa, trefoil, clovers, flax, elephant grass, grass, lettuce, sugar cane, tea, tobacco and coffee.
A further aspect of the invention relates to a kit or kit-of-parts comprising a first compo- nent which contains at least one component 1 ) comprising at least one viable microorganism (I) which is as defined herein, and optionally at least one conventional auxiliary; and at least one component 2) which contains the least one component (II) which is as defined herein, and optionally at least one conventional auxiliary.
A further aspect of the invention relates to plant seed, comprising a plant health composition as defined herein.
When preparing the compositions, it is preferred to employ the commercially available formulations of components 1 ) and 2), to which further compounds, as for example those active against harmful fungi or other pests, such as insects, arachnids or nematodes, or else herbicidal or growth-regulating active compounds or fertilizers may be added.
Usually, compositions comprising component 1 ) and 2), wherein component 2) consists of only one active ingredient (II), are employed. However, in certain cases compositions wherein component 2) consists of two or, if appropriate, more active components may be advantageous as well.
Depending on the particular components and the desired effect, the application rates for component 1 ) are generally from 0,01 I to 100 I broth containing the strain per hectare, preferably from 0,02 I to 50 I/ha, in particular from 0,05 to 10 I/ha.
Correspondingly, the application rates for component 2) are generally from 1 to 2000 g/ha, 5 to 100 g/ha, preferably from 10 to 500 g/ha, in particular from 40 to 250 g/ha of active ingredient each.
c) Component 1 ) (Microorganism) The plant health composition of the invention preferably comprises at least one microorganism (I) in its broadest sense, i.e. a microorganism in isolated (pure) form, a whole broth culture thereof, a supernatant of said culture, or a metabolite produced by cultivation of said microorganism, or a specific developmental stage, as for example conidia (asexual spores).
Suitable microorganisms may be selected from:
A) fungi, such as those of the genera Beaveήa spp. and Metarhizum spp., Tricho- derma spp., Gliocladium spp, Penicillium spp and Clonostachys spp.; as for example conidia thereof; and
B) bacteria such as those of the genera Pseudomonas spp., Bacillus spp.,; and CoI- letotrichum spp.; as well as Rhizobia selected from the family of Rhizobiaceae, as for example of the genus Bradyrhizobium spp. Examples of bacteria of the genus Bradyr- hizobium spp are B. japonicum and B. alkanii.
Non-limiting examples of suitable fungi are:
Fungi of the genus Trichoderma, in particular homokaryotic Trichoderma, and conidia thereof.
Examples of specific species are T. hazarium, T. viride and T. virens, and conidia thereof.
As non-limiting example of a specific fungal strain there may be mentioned: Trichoderma harzanium T-22 (available, for example, from Advanced Biological Marketing, Ohio, USA)
As non-limiting examples of commercially available preparations of beneficial bacterial microorganisms there may be mentioned products obtainable from Advanced Biological Marketing, Ohio, USA and sold under the following tradenames:
AMERICAS BEST INOCULANT ™ EXCALIBRE ™
EXCALIBRE QR™
each of which containing, according to the information provided by the manufacturer, a mixture of three different strains of Bradyrhizobia and being of particular use for the inoculation of soybeans and allowing to significantly increase crop yields. Further suitable bacterial strains are selected from Bacillus subtilis and Bacillus pumilus.
Suitable formulations of the Bacillus subtilis strain are commercially available under the tradenames RHAPSODY®, SERENADE® MAX and SERENADE® ASO from AgraQuest, Inc., USA.
A further suitable formulation of the Bacillus subtilis strain in combination with Bradyr- hizobia spp. is obtainable from Becker-Underwood under the tradename VAULT®.
Suitable formulations of the Bacillus pumilus strain are commercially available under the tradenames SONATA® and BALLAD® Plus from AgraQuest, Inc., USA.
d) Component 2) (Pesticides)
In general, active compounds Il may be present in different crystal modifications or stereoisomer forms, which may differ in biological activity. They also form part of the definition of component 2).
Subgroups A.1 to A.15 of insecticidal compounds:
A.1. Organo(thio)phosphates: acephate, azamethiphos, azinphos-methyl, chlor- pyrifos, chlorpyrifos-methyl, chlorfenvinphos, diazinon, dichlorvos, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidophos, methidathion, methyl-parathion, mevinphos, monocrotophos, oxydemeton-methyl, paraoxon, parathion, phenthoate, phosalone, phosmet, phosphamidon, phorate, phoxim, pirimiphos-methyl, profenofos, prothiofos, sul- prophos, tetrachlorvinphos, terbufos, triazophos, trichlorfon;
A.2. Carbamates: alanycarb, aldicarb, bendiocarb, benfuracarb, carbaryl, carbofu- ran, carbosulfan, fenoxycarb, furathiocarb, methiocarb, methomyl, oxamyl, pirimi- carb, propoxur, thiodicarb, triazamate;
A.3. Pyrethroids: allethrin, bifenthrin, cyfluthrin, cyhalothrin, cyphenothrin, cyper- methrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, imiprothrin, lambda- cyhalothrin, permethrin, prallethrin, pyrethrin I and II, resmethrin, silafluofen, tau- fluvalinate, tefluthrin, tetramethrin, tralomethrin, transfluthrin, profluthrin, dimeflu- thrin;
A.4. Growth regulators: a) chitin synthesis inhibitors: benzoylureas: chlorflua- zuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, no- valuron, teflubenzuron, triflumuron; buprofezin, diofenolan, hexythiazox, etoxa- zole, clofentazine; b) ecdysone antagonists: halofenozide, methoxyfenozide, te- bufenozide, azadirachtin; c) juvenoids: pyriproxyfen, methoprene, fenoxycarb; d) lipid biosynthesis inhibitors: spirodiclofen, spiromesifen, spirotetramat;
A.5. Nicotinic receptor agonists/antagonists compounds: clothianidin, dinotefuran, imidacloprid, thiamethoxam, nitenpyram, acetamiprid, thiacloprid; the thiazol compound of formula F1
Figure imgf000014_0001
A.6. GABA antagonist compounds: acetoprole, endosulfan, ethiprole, fipronil, va- niliprole, pyrafluprole, pyriprole, the phenylpyrazole compound of formula T2
Figure imgf000014_0002
A.7. Macrocyclic lactone insecticides: abamectin, emamectin, milbemectin, Ie- pimectin, spinosad;
A.8. METI I compounds: fenazaquin, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim;
A.9. METI Il and III compounds: acequinocyl, fluacyprim, hydramethylnon;
A.10. Uncoupler compounds: chlorfenapyr;
A.1 1. Oxidative phosphorylation inhibitor compounds: cyhexatin, diafenthiuron, fenbutatin oxide, propargite;
A.12. Moulting disruptor compounds: cyromazine; A.13. Mixed Function Oxidase inhibitor compounds: piperonyl butoxide;
A.14. Sodium channel blocker compounds: indoxacarb, metaflumizone,
A.15. Various: benclothiaz, bifenazate, cartap, flonicamid, pyridalyl, pymetrozine, sulfur, thiocyclam, flubendiamide, cyenopyrafen, flupyrazofos, cyflumetofen, ami- doflumet, the anthranilamide compounds of formula r3
Figure imgf000015_0001
wherein A1 is CH3, Cl, Br, I, X is C-H, C-Cl, C-F or N, Y' is F, Cl, or Br, Y" is hydrogen, F, Cl, CF3, B1 is hydrogen, Cl, Br, I, CN, B2 is Cl, Br, CF3, OCH2CF3, OCF2H, and RB is hydrogen, CH3 or CH(CH3)2, and the malononitrile compounds as described in JP 2002 284608, WO 02/89579, WO 02/90320, WO 02/90321 , WO 04/06677, WO 04/20399, JP 2004 99597, WO 05/68423, WO 05/68432, or WO 05/63694, especially the malononitrile compounds
CF2HCF2CF2CF2CH2C(CN)2CH2CH2CF3 (2-(2,2,3,3,4,4,5,5-octafluoropentyl)-2- (3,3,3-trifluoropropyl)malononitrile),
CF3(CH2)2C(CN)2CH2(CF2)5CF2H (2-(2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoro-heptyl)- 2-(3,3,3-trifluoro-propyl)-malononitrile), CF3(CH2)2C(CN)2(CH2)2C(CF3)2F (2-(3,4,4,4-tetrafluoro-3-trifluoromethyl-butyl)-2-
(3,3,3-trifluoro-propyl)-malononitrile),
CF3(CH2)2C(CN)2(CH2)2(CF2)3CF3 (2-(3,3,4,4,5,5,6,6,6-nonafluoro-hexyl)-2-(3,3,3- trifluoro-propyl)-malononitrile), CF2H(CF2)3CH2C(CN)2CH2(CF2)3CF2H (2,2-bis-(2,2,3,3,4,4,5,5-octafluoro-pentyl)- malononitrile),
CF3(CH2)2C(CN)2CH2(CF2)3CF3 (2-(2,2,3,3,4,4,5,5,5-nonafluoro-pentyl)-2-(3,3,3- trifluoro-propyl)-malononitrile),
CF3(CF2)2CH2C(CN)2CH2(CF2)3CF2H (2-(2,2,3,3,4,4,4-heptafluoro-butyl)-2-
(2,2,3,3,4,4,5,5-octafluoro-pentyl)-malononitrile) and CF3CF2CH2C(CN)2CH2(CF2)3CF2H (2-(2,2,3,3,4,4,5,5-octafluoro-pentyl)-2-
(2,2,3,3,3-pentafluoro-propyl)-malononitrile).
Subgroups B.1 to B.6 of fungicidal compounds: B.1. Azoles: cyproconazole, difenoconazole, epoxiconazole, fenbuconazole, flu- quinconazole, flutriafol, hexaconazole, ipconazole, metconazole, propiconazole, prothioconazole, tebuconazole, tetraconazole, triadimenol, triadimefon, triticona- zole, cyazofamid, imazalil, prochloraz, triflumizol, benomyl, carbendazim, thiaben- dazole, ethaboxam, and hymexazole.
B.2. Strobilurins: azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, metominostrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, methyl (2-chloro-5-[1-(3-methylbenzyloxyimino)ethyl]benzyl)carbamate, methyl (2- chloro-5-[1 -(6-methylpyridin-2-ylmethoxyimino)ethyl]benzyl)carbamate, and methyl 2-(ortho-((2,5-dimethylphenyloxymethylene)phenyl)-3-methoxyacrylate;
B.3. Carboxamides: boscalid, carboxin, benalaxyl, fenhexamid, flutolanil, furamet- pyr, metalaxyl, mefenoxam (metalaxyl-M), ofurace, oxadixyl, oxycarboxin, pen- thiopyrad, thifluzamide, tiadinil, dimethomorph, fluopicolide (picobenzamid), diclo- cymet, N-(4'-bromobiphenyl-2-yl)-4-difluoromethyl-2-methylthiazole-5- carboxamide, N-(4'-trifluoromethylbiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N-(4'-chloro-3'-fluorobiphenyl-2-yl)-4-di- fluoromethyl-2-methylthiazole-5-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2- yl)-3-difluoromethyl-1 -methylpyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-3-difluoromethyl-1-methylpyrazole-4-carboxamide; 3,4- dichloro-N-(2-cyanophenyl)isothiazol-5-carboxamide; N-(2',4'-difluorobiphenyl-2- yl)-1-methyl-3-trifluoromethyl-1 H-pyrazole- 4-carboxamide; N-(2',4'- dichlorobiphenyl-2-yl)-1 -methyl-3-trifluoromethyl-1 H-pyrazole- 4-carboxamide; N- (2',4'-difluorobiphenyl-2-yl)-3-difluoromethyl-1-methyl-1 H-pyrazole- 4- carboxamide; N-(2',4'-dichloro-biphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H- pyrazole-4-carboxamide; N-(2',5'-difluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl- 1 H-pyrazole-4-carboxamide; N-(2',5'-dichlorobiphenyl-2-yl)-1 -methyl-3- trifluoromethyl-1 H-pyrazole-4-carboxamide; N-(2',5'-difluorobiphenyl-2-yl)-3- difluoromethyl-1 -methyl-1 H-pyrazole-4-carboxamide; N-(2',5'-dichlorobiphenyl-2- yl)-3-di-fluoromethyl-1 -methyl-1 H-pyrazole- 4-carboxamide; N-(3',5'-difluoro- biphenyl-2-yl)-1-methyl-3-trifluoromethyl-1 H-pyrazole- 4-carboxamide; N-(3',5'- dichlorobiphenyl-2-yl)-1 -methyl-3-trifluoromethyl-1 H-pyrazole-4-carboxamide, N- (3',5'-difluorobiphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H-pyrazole-4-carboxamide; N-(3',5'-dichlorobiphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H-pyrazole-4- carboxamide; N-(3'-fluorobiphenyl-2-yl)-1 -methyl-3-trifluoro-methyl-1 H-pyrazole-4- carboxamide, N-(3'-chlorobiphenyl-2-yl)-1 -methyl-3-trifluoromethyl-1 H-pyrazole-4- carboxamide; N-(3'-fluorobiphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H-pyrazole- 4- carboxamide, N-(3'-chlorobiphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H-pyrazole-4- carboxamide; N-(2'-fluorobiphenyl-2-yl)-1-methyl-3-trifluoromethyl-1 H-pyrazole-4- carboxamide; N-(2'-chlorobiphenyl-2-yl)-1 -methyl-3-trifluoro-methyl-1 H-pyrazole- 4-carboxamide; N-(2'-fluorobiphenyl-2-yl)-3-difluoro-methyl-1 -methyl-1 H-pyrazole- 4-carboxamide; N-(2'-chlorbiphenyl-2-yl)-3-difluoromethyl-1 -methyl-1 H-pyrazole-4- carboxamide; N-(2'-fluoro-4'-chloro-5'-methylbiphenyl-2-yl)-1-methyl-3- trifluoromethyl-1 H-pyrazole-4-carbox-amide; N-(3',4',5'-trifluorobiphenyl-2-yl)-1- methyl-3-trifluoromethyl-1 H-pyrazole-4-carboxamide; N-(3',4',5'-trifluorobiphenyl- 2-yl)-1 -methyl-3-difluoromethyl-1 H-pyrazole-4-carboxamide; N-(2',4',5'- trifluorobiphenyl-2-yl)-1 -methyl-3-difluoromethyl-1 H-pyrazole-4-carboxamide; N- (3\4\5'-tri-fluorobiphenyl-2-yl)-3-chlorofluoromethyl-1 -methyl-1 H-pyrazole-4- carbox-amide; N-[2-(1 ,1 ,2,3,3,3-hexafluoropropoxy)phenyl]-1-methyl-3-trifluoro- methyl-1 H-pyrazole-4-carboxamide; N-[2-(1 ,1 , 2,3,3, 3-hexafluor-opropoxy)- phenyl]-3-difluoromethyl-1 -methyl-1 H-pyrazole-4-carboxamide, N-[2-(2-chloro- 1 ,1 ,2-trifluoroethoxy)phenyl]-1-methyl-3-trifluoromethyl-1 H-pyrazole-4- carboxamide; N-[2-(2-chlor-1 ,1 ,2-trifluoroethoxy)phenyl]-3-difluoromethyl-1- methyl-1 H-pyrazole-4-carboxamide; N-[2-(1 , 1 ,2,2-tetra-f luoroethoxy)phenyl]-3- difluoromethyl-1 -methyl-1 H-pyrazole-4-carbox-amide; N-[2-(1 ,1 ,2,2-tetrafluoro- ethoxy)phenyl]-1 -methyl-3-trifluoro-methyl-1 H-pyrazole-4-carboxamide; N-(4'- (trifluoromethylthio)biphenyl-2-yl)-3-di-fluoromethyl-1 -methyl-1 H-pyrazole-4- carboxamide; N-(4'-(trifluoromethyl-thio)biphenyl-2-yl)-1-methyl-3-trifluoromethyl- 1 H-pyrazole-4-carboxamide; and 5-fluoro-1 ,3-dimethyl-1 H-pyrazole-4-carboxylic acid [2-(1 ,2-dimethyl-propyl)-phenyl]-amide.
B.4. Heterocylic compounds: pyrimethanil, fenpiclonil, fludioxonil, aldimorph, do- demorph, fenpropimorph, tridemorph, iprodione, procymidone, famoxadone, fenamidone, octhilinone, probenazole, diclomezine, pyroquilon, proquinazid, tricy- clazole, captafol, captan, dazomet, fenoxanil, quinoxyfen, 5-chloro-7-(4- methylpiperidin-1 -yl)-6-(2,4,6-trifluorophenyl)-[1 ,2,4]triazolo[1 ,5-a]pyrimidine, 6- (3,4-dichloro-phenyl)-5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 6-(4-tert- butylphenyl)-5-methyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 5-methyl-6- (3,5,5-trimethyl-hexyl)-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 5-methyl-6-octyl-
[1 ,2,4]triazolo[1 ,5-a]pyrimi-dine-7-ylamine, 6-methyl-5-octyl-[1 ,2,4]triazolo[1 ,5- a]pyrimidine-7-ylamine, 6-ethyl-5-octyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 5-ethyl-6-octyl-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 5-ethyl-6-(3,5,5- trimethyl-hexyl)-[1 ,2,4]triazolo[1 ,5-a]pyrimidine-7-ylamine, 6-octyl-5-propyl- [1 ,2,4]tri-azolo[1 ,5-a]pyrimidine-7-ylamine, 5-methoxymethyl-6-octyl-[1 ,2,4]tri- azolo[1 ,5-a]pyrimidine-7-ylamine, 6-octyl-5-trifluoromethyl-[1 ,2,4]tri-azolo[1 ,5- a]pyrimidine-7-ylamine, and 5-trifluoromethyl-6-(3,5,5-trimethyl-hexyl)-[1 ,2,4]tri- azolo[1 ,5-a]pyrimidine-7-ylamine. B.5. Carbamates: mancozeb, maneb, metam, metiram, ferbam, propineb, thiram, zineb, ziram; diethofencarb, iprovalicarb, propamocarb, and methyl 3-(4- chlorophenyl)-3-(2-isopropoxycarbonylamino-3-methylbutyrylamino)propanoate.
B.6. Others: guazatine; streptomycin, validamycin A; binapacryl, dinocap, dinobu- ton; dithianon, isoprothiolane; fentin salts, such as fentin-acetate; edifenphos, iprobenfos, fosetyl, pyrazophos, chlorothalonil, dichlofluanid, flusulfamide, phthalide, quintozene, thiophanate-methyl, tolylfluanid; copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur; cyflufenamid, cymox- anil, dimethirimol, ethirimol, furalaxyl, metrafenone, and spiroxamine.
Subgroups of nematicidal compounds:
As non-limiting examples of nematicides there may be mentioned:
antibiotic nematicides, like abamectin;
carbamate nematicides, like benomyl, carbofuran, carbosulfan, cloethocarb;
oxime carbamate nematicides, like alanycarb, aldicarb, aldoxycarb, oxamyl;
organophosphate nematicides, like diamidafos, fenamiphas, fosthietan, phos- phamidon;
organothiophosphate nematicides, like cadusafos, chlorpyrifos, dichlofenthion, dimethoate, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofos, isa- zofos, phorate, phosphocarb, terbufos, thionazin, triazophos;
phosphonothioate nematicides, like imicyafos, mecarphon; as well as
acetoprole, benclothiaz, chloropicrin, dazomet, DBCP, DCIP, 1 ,2- dichloropropane, 1 ,3-dichloropropene, furfural, iodomethane, metam, methyl bromide, methyl isothiocyanate, xylenols.
Preferred fungicidal compounds selected from subgroups B.1 to B.6 are: metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebuconazole, triticonazole, iprodion, meti- ram, thiram, boscalid, carbendazim, silthiofam, fludioxonil, azoxystrobin, ethaboxam, kresoxim-methyl, orysastrobin, pyraclostrobin trifloxystrobin, thiophante methyl.
Particularely preferred fungicides are: metalaxyl, epoxiconazole, fluquiconazole, prochloraz, triticonazole, iprodion, thiram, tebuconazole, boscalid, carbendazim, silthiofam, fludioxonil, azoxystrobin, orysastrobin, pyraclostrobin trifloxystrobin, thiophante methyl, ethaboxam, metconazole.
Preferred insecticides selected from subgroups A.1 to A.15 are: acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidacloprid, spinosad, tefluthrin, thiamethoxam, metaflumizon.
Particularely preferred insecticides are: acetamiprid, clothianidin, fipronil, imidacloprid, spinosad, thiamethoxam, metaflumizon
e) Non-limiting examples of suitable combinations of components 1 ) and 2):
AMERICAS BEST INOCULANT ™ combined with at least one, like one or two, preferably one member of the group of metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebu- conazole, triticonazole, iprodion, metiram, thiram, boscalid, carbendazim, silthiofam, fludioxonil, azoxystrobin, ethaboxam , kresoxim-methyl, orysastrobin, pyraclostrobin trifloxystrobin, thiophante methyl.
EXCALIBRE ™ combined with at least one, like one or two, preferably one member of the group of metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebuconazole, triticonazole, iprodion, metiram, thiram, boscalid, carbendazim, silthiofam, fludioxonil, azoxystrobin, ethaboxam, kresoxim-methyl, orysastrobin, pyraclostrobin trifloxystrobin, thiophante methyl.
EXCALIBRE QR™ combined with at least one, like one or two, preferably one member of the group of metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebuconazole, triticonazole, iprodion, metiram, thiram, boscalid, carbendazim, silthiofam, fludioxonil, azox- ystrobin, ethaboxam, kresoxim-methyl, orysastrobin, pyraclostrobin trifloxystrobin, thio- phante methyl.
VAULT® combined with at least one, like one or two, preferably one member of the group of metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebuconazole, triticonazole, iprodion, metiram, thiram, boscalid, carbendazim, silthiofam, fludioxonil, azoxystrobin, ethaboxam, kresoxim-methyl, orysastrobin, pyraclostrobin trifloxystrobin, thiophante methyl.
AMERICAS BEST INOCULANT ™ combined with at least one, like one or two, preferably one member of the group of acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidacloprid, spinosad, tefluthrin, thiamethoxam, metaflumizon.
EXCALIBRE ™ combined with at least one, like one or two, preferably one member of the group of acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidacloprid, spinosad, tefluthrin, thiamethoxam, metaflumizon.
EXCALIBRE QR™ combined with at least one, like one or two, preferably one member of the group acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidacloprid, spinosad, tefluthrin, thiamethoxam, metaflumizon.
VAULT® combined with at least one, like one or two, preferably one member of the group of acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidacloprid, spinosad, tefluthrin, thiamethoxam, metaflumizon.
f) Preparation of microorganism formulation (component D)
The particulate formulation comprising at least one viable, beneficial microorganism may be prepared in different ways, applying standard formulation techniques of mixing, drying and optionally coating (see for example Mollet, H. et al., Formulierungstechnik, WILEY-VCH Verlag GmbH, Weinheim, Germany (2000)). In general particles may be generated the mean particle size of which may vary in a wide range of 0,01 to 10 mm, as for example 0,1 to 5 mm or 0,1 to 2 mm or 0.2 to 1 mm.
The preparation techniques and constituents applied allow the preparation of particles characterized by a high content of viable microorganisms characterized by good stor- age stability of the viable microorganisms. Typically, formulations having cfu values in the range of 106 to about 1012, or 5 x 108 to about 1012 are made available.
Typically, the water content of such particulate formulations is significantly low. The water content, expressed via the water activity parameter aw, is below about 1.0, in particular below about 0,4 or below about 0,2 or below about 0,1 or even below about 0,01. Examples of such aw parameter ranges are 0 to 1 , 0,0001 to 0,2, 0,001 to 0,15 or 0,002 to 0,10 or 0,002 to 0,1.
f1 ) Preparation of microorganism formulation comprising a water insoluble, water- adsorbent carrier
A suitable method of preparation is for example disclosed in WO 2007/030557 the disclosure of which is incorporated by reference.
A water insoluble, water-absorbent substance is combined with the aqueous suspension of viable microorganisms to an amount of about 80 to 99% by total weight of the formulation. The aqueous suspension of viable microorganism is present in the formulation in an amount of about 1 to 20% by total weight of the formulation. Preferably, the water insoluble, water-absorbent substance is present in the formulation in the amount of at least about 80%, 85%, 90%, 95%, or 99% by total weight of the formulation. Combining the aqueous suspension of viable microorganisms with the water insoluble, water-absorbent substance can be carried out by a variety of methods. The combining step may be carried out by kneading the aqueous suspension with the water insoluble, water absorbent substance in a flexible container or bag.
f2) Preparation of microorganism formulation comprising a water soluble carrier
Suitable methods of preparation is for example disclosed in WO 99/57242 or US 7,037,708 the disclosure of which documents is incorporated by reference.
Said methods make use of well-known spray-drying, freeze-drying of fluidized bed- drying processes to obtain a dry powder of carrier-bound viable microorganisms, optionally further combined with subsequent steps of agglomerating, tabletting and/or coating.
For example, a spray-drying process for producing a dry microorganism culture, comprises a) dissolving or suspending at least one substance suitable for forming a carrier in a liquid comprising at least one microorganism species b) drying the resultant mixture in a spray-dryer, for the spray-drying use being made of a conditioned dried gas heated to a temperature in the range of above about 8O0C, in particular from about 90 to about 1350C, preferably from about 100 to about 11 O0C, such as about 1050C, and c) removing the dried material from the spray-dryer, this dried material having an exit temperature of from about 40 to 850C, in particular from about 45 to 750C, preferably from about 50 to 650C, such as about 550C.
The gas used for the drying is preferably a dried gas having a dew point of below +50C, in particular having a dew point of from about -10 to about -5O0C, such as conditioned compressed air or conditioned nitrogen. For example, compressed air having a dew point of about -250C and nitrogen having a dew point of about -4O0C can be used. A dew point of +50C is equivalent to roughly 5 g of water per m3 of air.
According to a further embodiment of the spray-drying process, in a downstream further stage d), the dried material is subjected to a post-drying. The post-drying temperature is in the range of from about 15 to 5O0C, such as from about 25 to 4O0C. The post- drying is performed, for example, in a gas atmosphere or in vacuo; alternatively to this, there is also the possibility of mixing a desiccant homogeneously with the dry microorganism preparation obtained in accordance with stage c).
The material to be sprayed may have a solids content (after addition of the carrier) of from about 10 to 40, such as from about 10 to 25% by weight.
The use of preconditioned, i.e. low-moisture, drying air is of advantage. Preferably, use is made of compressed air having a dew point at about -250C.
The spray-drying may for example be carried out in a laboratory spray-dryer of type Niro Minor from Niro, Copenhagen, Denmark. The ready-to-spray bacterial suspension is sprayed via a two-component nozzle cocurrently with preconditioned heated compressed air into the plant drying tower, the dried product is separated from the air using a cyclone and collected.
d) Plant health formulations
The plant health compositions of the invention can be converted into the customary formulations (or agents), for example solutions, emulsions, suspensions, dusts, pow- ders, pastes and granules. The use form depends on the particular intended purpose; in each case, it should ensure a fine and even distribution of the compounds according to the invention. The formulations are prepared in a known manner (see e.g. for review US 3,060,084, EP-A 707 445 (for liquid concentrates), Browning, 'Agglomeration', Chemical Engineering, Dec. 4, 1967, 147-48, Perry's Chemical Engineer's Handbook, 4th Ed., McGraw-Hill, New York, 1963, pages 8-57 and et seq. WO 91/13546, US 4,172,714, US 4,144,050, US 3,920,442, US 5,180,587, US 5,232,701 , US 5,208,030, GB 2,095,558, US 3,299,566, Klingman, Weed Control as a Science, John Wiley and Sons, Inc., New York, 1961 , Hance et al., Weed Control Handbook, 8th Ed., Blackwell Scientific Publications, Oxford, 1989 and Mollet, H., Grubemann, A., Formulation technology, Wiley VCH Verlag GmbH, Weinheim (Germany), 2001 , 2. D. A. Knowles, Chemistry and Technology of Agrochemical Formulations, Kluwer Academic Publishers, Dordrecht, 1998 (ISBN 0-7514-0443-8), for example by extending the active compound with auxiliaries suitable for the formulation of agrochemicals, such as solvents and/or carriers, if desired emulsifiers, surfactants and dispersants, preservatives, anti- foaming agents, anti-freezing agents, for seed treatment formulation also optionally colorants and/or binders and/or gelling agents.
Examples of suitable solvents are water, aromatic solvents (for example Solvesso products, xylene), paraffins (for example mineral oil fractions), alcohols (for example methanol, butanol, pentanol, benzyl alcohol), ketones (for example cyclohexanone, gamma-butyrolactone), pyrrolidones (NMP, NOP), acetates (glycol diacetate), glycols, fatty acid dimethylamides, fatty acids and fatty acid esters. In principle, solvent mixtures may also be used. Suitable emulsifiers are nonionic and anionic emulsifiers (for example polyoxyethylene fatty alcohol ethers, alkylsulfonates and arylsulfonates).
Examples of dispersants are lignin-sulfite waste liquors and methylcellulose.
Suitable surfactants used are alkali metal, alkaline earth metal and ammonium salts of lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalene- sulfonic acid, alkylarylsulfonates, alkyl sulfates, alkylsulfonates, fatty alcohol sulfates, fatty acids and sulfated fatty alcohol glycol ethers, furthermore condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, condensates of naphthalene or of naphthalenesulfonic acid with phenol and formaldehyde, poly- oxyethylene octylphenol ether, ethoxylated isooctylphenol, octylphenol, nonylphenol, alkylphenol polyglycol ethers, tributylphenyl polyglycol ether, tristearylphenyl polyglycol ether, alkylaryl polyether alcohols, alcohol and fatty alcohol ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers, ethoxylated polyoxypropyl- ene, lauryl alcohol polyglycol ether acetal, sorbitol esters, lignosulfite waste liquors and methylcellulose. Substances which are suitable for the preparation of directly sprayable solutions, emulsions, pastes or oil dispersions are mineral oil fractions of medium to high boiling point, such as kerosene or diesel oil, furthermore coal tar oils and oils of vegetable or animal origin, aliphatic, cyclic and aromatic hydrocarbons, for example toluene, xylene, paraffin, tetrahydronaphthalene, alkylated naphthalenes or their derivatives, methanol, etha- nol, propanol, butanol, cyclohexanol, cyclohexanone, isophorone, highly polar solvents, for example dimethyl sulfoxide, N-methylpyrrolidone or water.
Also anti-freezing agents such as glycerine, ethylene glycol, propylene glycol and bactericides such as can be added to the formulation.
Suitable antifoaming agents are for example antifoaming agents based on silicon or magnesium stearate.
Suitable preservatives are for example dichlorophen und benzylalkoholhemiformal. Seed Treatment formulations may additionally comprise binders and optionally colorants.
Binders can be added to improve the adhesion of the active materials on the seeds after treatment. Suitable binders are homo- and copolymers from alkylene oxides like ethylene oxide or propylene oxide, polyvinylacetate, polyvinylalcohols, polyvinylpyrrolidones, and copolymers thereof, ethylene-vinyl acetate copolymers, acrylic homo- and copolymers, polyethyleneamines, polyethyleneamides and polyethyleneimines, poly- saccharides like cellulose, modified cellulose, tylose and starch, polyolefin homo- and copolymers like olefin/maleic anhydride copolymers, polyurethanes, polyesters, polystyrene homo and copolymers.
Optionally, also colorants can be included in the formulation. Suitable colorants or dyes for seed treatment formulations are Rhodamin B, C.I. Pigment Red 112, C.I. Solvent Red 1 , pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1 , pigment blue 80, pigment yellow 1 , pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1 , pigment red 57:1 , pigment red 53:1 , pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51 , acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108.
Examples of a gelling agent is carrageen (Satiagel®) Powders, materials for spreading and dustable products can be prepared by mixing or concomitantly grinding the active substances with a solid carrier.
Granules, for example coated granules, impregnated granules and homogeneous granules, can be prepared by binding the active compounds to solid carriers.
Examples of solid carriers are mineral earths such as silica gels, silicates, talc, kaolin, attaclay, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetic materials, fertiliz- ers, such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin, such as cereal meal, tree bark meal, wood meal and nutshell meal, cellulose powders and other solid carriers.
In general, the formulations comprise from 0.01 to 95% by weight, preferably from 0.1 to 90% by weight, of the active compound(s). In this case, the active compound(s) are employed in a purity of from 90% to 100% by weight, preferably 95% to 100% by weight (according to NMR spectrum).
For seed treatment purposes, respective formulations can in certain cases be diluted 2- 10 fold leading to concentrations in the ready to use preparations of 0,01 to 60% by weight active compound by weight, preferably 0,1 to 40% by weight.
The plant health compositions of the invention can be used as such, in the form of their formulations or the use forms prepared there from, for example in the form of directly sprayable solutions, powders, suspensions or dispersions, emulsions, oil dispersions, pastes, dustable products, materials for spreading, or granules, by means of spraying, atomizing, dusting, spreading or pouring. The use forms depend entirely on the intended purposes; they are intended to ensure in each case the finest possible distribution of the active compound(s) according to the invention.
Aqueous use forms can be prepared from emulsion concentrates, pastes or wettable powders (sprayable powders, oil dispersions) by adding water. To prepare emulsions, pastes or oil dispersions, the substances, as such or dissolved in an oil or solvent, can be homogenized in water by means of a wetter, tackifier, dispersant or emulsifier. However, it is also possible to prepare concentrates composed of active substance, wetter, tackifier, dispersant or emulsifier and, if appropriate, solvent or oil, and such concentrates are suitable for dilution with water. The active compound concentrations in the ready-to-use preparations can be varied within relatively wide ranges. In general, they are from 0.0001 to 10%, preferably from 0.01 to 1% per weight. The active compound(s) may also be used successfully in the ultra-low-volume process (ULV), it being possible to apply formulations comprising over 95% by weight of active compound, or even to apply the active compound without additives.
e) Examples of suitable formulations:
1. Products for dilution with water for spray applications. For seed treatment purposes, such products may be applied to the seed diluted or undiluted.
A) Water-soluble concentrates (SL, LS)
10 parts by weight of the active compound(s) are dissolved in 90 parts by weight of water or a water-soluble solvent. As an alternative, wetteners or other auxiliaries are added. The active compound(s) dissolves upon dilution with water, whereby a formula- tion with 10 % (w/w) of active compound(s) is obtained.
B) Dispersible concentrates (DC)
20 parts by weight of the active compound(s) are dissolved in 70 parts by weight of cyclohexanone with addition of 10 parts by weight of a dispersant, for example polyvinylpyrrolidone. Dilution with water gives a dispersion, whereby a formulation with 20% (w/w) of active compound(s) is obtained.
C) Emulsifiable concentrates (EC)
15 parts by weight of the active compound(s) are dissolved in 7 parts by weight of an organic solvent, for example an alkylaromatic solvent with addition of calcium dodecyl- benzenesulfonate and castor oil ethoxylate (in each case 5 parts by weight). Dilution with water gives an emulsion, whereby a formulation with 15% (w/w) of active com- pound(s) is obtained.
D) Emulsions (EW, EO, ES)
25 parts by weight of the active compound(s) are dissolved in 35 parts by weight of an organic solvent, for example an alkylaromatic solvent with addition of calcium dodecyl- benzenesulfonate and castor oil ethoxylate (in each case 5 parts by weight). This mixture is introduced into 30 parts by weight of water by means of an emulsifier machine (e.g. Ultraturrax) and made into a homogeneous emulsion. Dilution with water gives an emulsion, whereby a formulation with 25% (w/w) of active compound(s) is obtained. E) Suspensions (SC, OD, FS)
In an agitated ball mill, 20 parts by weight of the active compound(s) are comminuted with addition of 10 parts by weight of dispersants, wetteners and 70 parts by weight of water or of an organic solvent to give a fine active compound(s) suspension. Dilution with water gives a stable suspension of the active compound(s), whereby a formulation with 20% (w/w) of active compound(s) is obtained.
F) Water-dispersible granules and water-soluble granules (WG, SG)
50 parts by weight of the active compound(s) are ground finely with addition of 50 parts by weight of dispersants and wetteners and made as water-dispersible or water-soluble granules by means of technical appliances (for example extrusion, spray tower, fluid- ized bed). Dilution with water gives a stable dispersion or solution of the active com- pound(s), whereby a formulation with 50% (w/w) of active compound(s) is obtained.
G) Water-dispersible powders and water-soluble powders (WP, SP, SS, WS)
75 parts by weight of the active compound(s) are ground in a rotor-stator mill with addi- tion of 25 parts by weight of dispersants, wetteners and silica gel. Dilution with water gives a stable dispersion or solution of the active compound(s) , whereby a formulation with 75% (w/w) of active compound(s) is obtained.
Gel-Formulation (GF) (for seed treatment purposes only)
In an agitated ball mill, 20 parts by weight of the active compound(s) are comminuted with addition of 10 parts by weight of dispersants, 1 part by weight of a gelling agent wetters and 70 parts by weight of water or of an organic solvent to give a fine active compound(s) suspension. Dilution with water gives a stable suspension of the active compound(s), whereby a formulation with 20% (w/w) of active compound(s) is obtained.
2. Products to be applied undiluted for foliar applications. For seed treatment purposes, such products may be applied to the seed diluted or undiluted:
I) Dustable powders (DP, DS)
5 parts by weight of the active compound(s) are ground finely and mixed intimately with 95 parts by weight of finely divided kaolin. This gives a dustable product having 5% (w/w) of active compound(s). J) Granules (GR, FG, GG, MG)
0.5 part by weight of the active compound(s) is ground finely and associated with 95.5 parts by weight of carriers, whereby a formulation with 0.5% (w/w) of active compound^) is obtained. Current methods are extrusion, spray-drying or the fluidized bed. This gives granules to be applied undiluted for foliar use.
K) ULV solutions (UL)
10 parts by weight of the active compound(s) are dissolved in 90 parts by weight of an organic solvent, for example an alkylaromatic solvent. This gives a product having 10% (w/w) of active compound(s), which is applied undiluted for foliar use.
f) Seed treatment:
Conventional seed treatment formulations include for example flowable concentrates FS, solutions LS, powders for dry treatment DS, water dispersible powders for slurry treatment WS, water-soluble powders SS and emulsion ES and EC and gel formulation GF. These formulations can be applied to the seed diluted or undiluted. Application to the seeds is carried out before sowing, either directly on the seeds.
In one embodiment a FS formulation is used for seed treatment. Typcially, a FS formulation may comprise 1-800 g/l of active ingredient, 1-200 g/l surfactant, 0 to 200 g/l anti- freezing agent, 0 to 400 g/l of binder, 0 to 200 g/l of a pigment and up to 1 liter of a solvent, preferably water.
In accordance with one variant of the present invention, a further subject of the invention is a method of treating soil by the application, in particular into the seed drill: either of a granular formulation containing the plant health composition of the invention - in combination or as a composition/formulation, or of a mixture of two granular formulations, each containing one of the two active ingredients, with optionally one or more solid or liquid, agriculturally acceptable carriers and/or optionally with one or more agriculturally acceptable surfactants. This method is advantageously employed in seed- beds of cereal, maize, cotton and sunflower. The rates for each active ingredient may be in the range of 10 tolOOO g/ha, as for example 50 to 500 g/ha or 50 to 200 g/ha.
The present invention also comprises seeds coated with or containing a plant health composition of the invention. Suitable seed is seed of cereals, root crops, oil crops, vegetables, spices, ornamentals, for example seed of durum and other wheat, barley, oats, rye, maize (fodder maize and sugar maize / sweet and field corn), soybeans, oil crops, crucifers, cotton, sunflowers, bananas, rice, oilseed rape, turnip rape, sugarbeet, fodder beet, eggplants, potatoes, grass, lawn, turf, fodder grass, tomatoes, leeks, pumpkin/squash, cabbage, iceberg lettuce, pepper, cucumbers, melons, Brassica species, melons, beans, peas, garlic, onions, carrots, tuberous plants such as potatoes, sugar cane, tobacco, grapes, petunias, geranium/pelargoniums, pansies and impatiens.
In addition, the plant health compositions of the invention may also be used for the treatment seeds from plants, which tolerate the action of herbicides or fungicides or insecticides owing to breeding, including genetic engineering methods.
For example, the plant health compositions of the invention may be applied to trans- genie crops which are resistant to herbicides from the group consisting of the sulfonylureas (EP-A-0257993, U.S. Pat. No. 5,013,659), imidazolinones (see for example US 6222100, WO0182685, WO0026390, WO9741218, WO9802526, WO9802527, WO 04/106529, WO 05/20673, WO 03/14357, WO 03/13225, WO 03/14356, WO 04/16073), glufosinate-type (see for example EP-A-0242236, EP-A-242246) or gly- phosate-type (see for example WO 92/00377) or plants resistant towards herbicides selected from the group of cyclohexadienone/Aryloxyphenoxypropionic acid herbicides (US 5,162,602 , US 5,290,696 , US 5,498,544 , US 5,428,001 , US 6,069,298 , US 6,268,550 , US 6,146,867 , US 6,222,099 , US 6,414,222) or transgenic crop plants, for example cotton, with the capability of producing Bacillus thuringiensis toxins (Bt toxins) which make the plants resistant to certain pests (E P-A-0142924, E P-A-0193259). Furthermore, the plant health compositions of the invention can be used also for the treatment of seeds from plants, which have modified characteristics in comparison with existing plants consist, which can be generated for example by traditional breeding methods and/or the generation of mutants, or by recombinant procedures. For example, a number of cases have been described of recombinant modifications of crop plants for the purpose of modifying the starch synthesized in the plants (e.g. WO 92/1 1376, WO 92/14827, WO 91/19806) or of transgenic crop plants having a modified fatty acid composition (WO 91/13972). The seed treatment application of the plant health compositions of the invention is carried out by spraying or dusting the seeds before sowing of the plants and before emergence of the plants.
In the treatment of seeds the corresponding formulations are applied by treating the seeds with an effective amount of the plant health composition of the invention. Herein, the application rates of the plant health composition of the invention are generally from 0,1 g to 10 kg per 100 kg of seed, preferably from 1 g to 5 kg per 100 kg of seed, in particular from 1 g to 2,5 kg per 100 kg of seed. For specific crops such as lettuce the rate can be higher.
g) Control of phytopathoqens
The plant health compositions may be suitable for controlling the following phytopatho- genic fungi:
Alternaria atrans tenuissima Fusarium solani
Alternaria brassicae Fusarium culmorum
Alternaria spp. Fusarium graminearum
Ascochyta tritici Gaeumannomyces graminis
Blumeria graminis Leptosphaeria nodorum
Botrytis cinerea Leptosphaerulina trifolii
Bremia lactucae Macrophomina phaseolina
Bremia lucinae Microdochium nivale
Calonectria crotalariae Microsphaera diffusa
Cercospora canescens Mycoleptodiscus terrestris
Cercospora kikuchii Neocosmospora vasinfecta
Cercospora sojina Pellicularia sasakii
Cercospora canescens Peronospora brassicae
Choanephora infundibulifera Peronospora manshurica
Cladosporium herbarum Peronospora brassicae
Cochliobolus sativus Peronospora pisi
Cochliobolus sativus Phakopsora pachyrhizi
Colletotrichum truncatum Phakopsora meibomiae
Corynespora cassiicola Phialophora gregata
Dactuliophora glycines Phomopsis phaseoli
Dematophora necatrix Phyllostica sojaecola
Diaporthe phaseolorum Physiological leaf spots
Diaporthe phaseolorum var. caulivora Phythium ultimum
Drechslera glycini Phytophthora megasperma
Epicoccum spp. Phytophthora infestans
Erwinia amylovora Phytopthora megasperma
Erysiphe graminis Plasmopara viticola
Frogeye sojina Podosphaera leucotricha Podosphaera leucotricha Pyrenophora graminea Pseudocercospora herpotrichoides Pyrenophora graminea Pseudomonas lachrymans Pyrenophora heraclei Pseudomonas syringae Pyrenophora hordei Pseudoperonospora cubensis Pyrenophora horrida Pseudoperonospora humuli Pyrenophora hyperici Puccinia hordei Pyrenophora japonica Puccinia recondita Pyrenophora kugitangi Puccinia striiformis Pyrenophora lithophila Puccinia triticina Pyrenophora lolii Pyrenochaeta glycines Pyrenophora macrospora Pyrenophora allosuri Pyrenophora metasequoiae Pyrenophora altermarina Pyrenophora minuertiae hirsutae Pyrenophora avenae Pyrenophora moravica Pyrenophora bartramiae Pyrenophora moroczkowskii Pyrenophora bondarzevii Pyrenophora muscorum Pyrenophora bromi Pyrenophora osmanthi Pyrenophora bryophila Pyrenophora phlei Pyrenophora buddleiae Pyrenophora pimpinellae Pyrenophora bupleuri Pyrenophora pittospori Pyrenophora calvertii Pyrenophora polytricha Pyrenophora calvescens var. moravica Pyrenophora pontresinerisis Pyrenophora carthanie Pyrenophora pulsatillae Pyrenophora centranthi Pyrenophora raetica Pyrenophora cerastii Pyrenophora rayssiae Pyrenophora chengii Pyrenophora rugosa Pyrenophora chrysamthemi Pyrenophora ryohicola Pyrenophora convohuli Pyrenophora saviczii Pyrenophora coppeyana Pyrenophora schoeteri Pyrenophora cytisi Pyrenophora scholevskii Pyrenophora dactylidis Pyrenophora scirpi Pyrenophora dictyoides Pyrenophora scirpicola Pyrenophora echinopis Pyrenophora secalis Pyrenophora ephemera Pyrenophora semeniperda Pyrenophora eryngicola Pyrenophora semiusta Pyrenophora erythrospila Pyrenophora seseli Pyrenophora euphorbiae Pyrenophora seseli f. poterii Pyrenophora freticola Pyrenophora subalpina Pyrenophora sudetica Rhizoctonia choussii Pyrenophora suhantarctica Rhizoctonia coniothecioides Pyrenophora syntrichiae Rhizoctonia cundida Pyrenophora szaferiana Rhizoctonia dichoroma Pyrenophora teres Rhizoctonia dimorpha Pyrenophora teres f. makulata Rhizoctonia endophytica Pyrenophora teres subsp. graminea Rhizoctonia endophytica vor. filicata Pyrenophora tetrahenae Rhizoctonia ferruginea Pyrenophora tranzschelii Rhizoctonia floccosa Pyrenophora trifulii Rhizoctonia fragariae Pyrenophora triticil-repentis Rhizoctonia fraxini Pyrenophora ushuwaiensis Rhizoctonia fuliginea Pyrenophora villose Rhizoctonia fumigata Pyrenophora graminea Rhizoctonia globularis Pyrenophora teres Rhizoctonia goodyerae-repentis Pyrenophora teres Rhizoctonia gossypii Pyrenophora teres Rhizoctonia gossypii vor. anatolica Pyrenophora tritici repentis Rhizoctonia gracilis Pyricularia oryzae Rhizoctonia griseo Pythium aphanidermatum Rhizoctonia hiemalis Pythium debaryanum Rhizoctonia juniperi Pythium irregulare Rhizoctonia lamallifera Pythium myriotylum Rhizoctonia leguminicola Pythium ultimum Rhizoctonia lilacina Ramularia collocygni Rhizoctonia luoini Rhizoctonia aerea Rhizoctonia macrosclerotia Rhizoctonia alba Rhizoctonia melongenae Rhizoctonia alpina Rhizoctonia microsclerotia Rhizoctonia anaticula Rhizoctonia monilioides Rhizoctonia anomala Rhizoctonia monteithiana Rhizoctonia apocynacearum Rhizoctonia muneratii Rhizoctonia arachnion Rhizoctonia nandorii Rhizoctonia asclerotica Rhizoctonia oryzae Rhizoctonia batalicola Rhizoctonia oryzae-sativae Rhizoctonia borealis Rhizoctonia pallida Rhizoctonia callae Rhizoctonia pini-insignis Rhizoctonia carorae Rhizoctonia praticola Rhizoctonia cerealis Rhizoctonia quercus Rhizoctonia ramicola Tilletia armdinellae
Rhizoctonia robusta Tilletia asperifolia
Rhizoctonia rubi Tilletia asperitolioides
Rhizoctonia ruhiginosa Tilletia atacamensis
Rhizoctonia sclerotica Tilletia baldrati
Rhizoctonia solani Tilletia bambusae
Rhizoctonia solani f. paroketea Tilletia banarasae
Rhizoctonia solani forma specialis Tilletia bangalorensis
Rhizoctonia solani var. cedri-deodorae Tilletia barclayana
Rhizoctonia solani var. fuchsiae Tilletia biharica
Rhizoctonia solani var. hortensis Tilletia boliviensis
Rhizoctonia stahlii Tilletia boutelouae
Rhizoctonia subtilis var. nigra Tilletia brachypodii
Rhizoctonia subtlilis Tilletia brachypodii-ramosi
Rhizoctonia tomato Tilletia braomi-tectorum
Rhizoctonia tuliparum Tilletia brevifaciens
Rhizoctonia veae Tilletia bromi
Rhizoctonia versicolor Tilletia bromina
Rhizoctonia cerealis Tilletia brunkii
Rhynchosporium secalis Tilletia buchloeana
Sclerotina rolfsii Tilletia bulayi
Sclerotinia rolfsii Tilletia caries
Sclerotinia sclerotiorum Tilletia cathcariae
Septoria glycines Tilletia cerebrina
Septoria nodorum Tilletia chloridicola
Septoria tritici Tilletia contaoversa
Sphaerotheca fuliginea Tilletia contraversa var. prostata
Stagonospora nodorum Tilletia contraversa var. elyni
Stemphylium botryosum Tilletia corona
Thielaviopsis basicola Tilletia cynasuri
Tilletia aegilopis Tilletia damacarae
Tilletia aegopogonis Tilletia deyeuxiae
Tilletia ahamadiana Tilletia digitariicola
Tilletia airina Tilletia durangensis
Tilletia ajrekari Tilletia earlei
Tilletia alopecuri Tilletia echinochlave
Tilletia anthaxanthi Tilletia echinochloae
Tilletia apludae Tilletia echinosperma Tilletia ehrhartae Tilletia maglagonii
Tilletia eleusines Tilletia makutensis
Tilletia elymandrae Tilletia milti
Tilletia elymicola Tilletia milti-vernalis
Tilletia elyni Tilletia montana
Tilletia elythrophori Tilletia montemartinii
Tilletia eragrostidis Tilletia nanifica
Tilletia euphorbiae Tilletia narasimhanii
Tilletia fahrendorfii Tilletia narayanaoana
Tilletia festinca-octoflorana Tilletia narduri
Tilletia foelida Tilletia nigrifaciens
Tilletia foliicola Tilletia obscura-reticulora
Tilletia fusca Tilletia oklahomae
Tilletia fusca var. bromi-tectorum Tilletia okudoirae
Tilletia fusca var. guyotiana Tilletia oplistneni-cristati
Tilletia fusca var.paragonica Tilletia paae
Tilletia georfischeri Tilletia pachy derma
Tilletia gigaspora Tilletia pallida
Tilletia goloskokovii Tilletia panici
Tilletia haynaldiae Tilletia panici. humilis
Tilletia heterospora Tilletia paonensis
Tilletia hold Tilletia paraloxa
Tilletia hordei var.spontanei Tilletia paspali
Tilletia horrida Tilletia pennisetina
Tilletia hyalospora var.cuzcoensis Tilletia peritidis
Tilletia hyparrheniae Tilletia phalaridis
Tilletia indica Tilletia polypoganis
Tilletia iniermedia Tilletia prostata
Tilletia iovensis Tilletia pulcherrima var. brachiariae
Tilletia ixophari Tilletia redfieldiae
Tilletia koeleriae Tilletia rhei
Tilletia kuznetzoviana Tilletia rugispora
Tilletia laevis Tilletia sabaudiae
Tilletia laguri Tilletia salzmanii
Tilletia leptochlase Tilletia savilei
Tilletia lepturi Tilletia scrobiculata
Tilletia macrotuberculata Tilletia setariae
Tilletia madeirensis Tilletia setariae-palmiflorarae Tilletia setariicola Ustilago almadina Tilletia sphaerococca Ustilago alopecurivara Tilletia sphenopie Ustilago alsineae Tilletia sphenopodis Ustilago altilis Tilletia steήlis Ustilago amadelpha var. glabriuscula Tilletia taiana Ustilago amphilophidis Tilletia texana Ustilago amplexa
Tilletia themedae-anatherae Ustilago amthoxanthi Tilletia themedicola Ustilago andropogonis-tectorum Tilletia toguateei Ustilago aneilemae Tilletia trachypogonis Ustilago anhweiona Tilletia transiliensis Ustilago anomala var. avicularis Tilletia transvaalensis Ustilago anomala var. carnea Tilletia tritici f. monococci Ustilago anomala var. cordai Tilletia tritici var. controversa Ustilago anomala var. microspora Tilletia tritici var. nanifica Ustilago anomala var. muricata Tilletia tritici var. laevis Ustilago anomala var. tovarae Tilletia tritici-repentis Ustilago apscheronica Tilletia triticoides Ustilago arabidia.alpinae Tilletia tuberculare Ustilago arandinellae-hirtae Tilletia vertiveriae Ustilago arctica Tilletia viermotii Ustilago argentina Tilletia vittara Ustilago aristidarius
Tilletia vittara var. burmahnii Ustilago arotragostis Tilletia walkeri Ustilago asparagi-pygmaei Tilletia youngii Ustilago asprellae Tilletia zundelii Ustilago avanae subsp. alba Typhula incarnata Ustilago avenae Uromyces appendiculatus Ustilago avenae Ustilago aaeluropodis Ustilago avenae f. sp. perennars Ustilago abstrusa Ustilago avenariae-bryophyllae Ustilago aegilopsidis Ustilago avicularis Ustilago affinis var. hilariae Ustilago bahuichivoensis Ustilago agrestis Ustilago barbari Ustilago agropyrina Ustilago beckeropsis Ustilago agrostis-palustris Ustilago belgiana Ustilago airear-caespitosae Ustilago bethelii Ustilago alismatis Ustilago bicolor Ustilago bistortarum ustiloginea Ustilago clintoniana
Ustilago bistortarum var. pustulata Ustilago coloradensis
Ustilago boreatis Ustilago commelinae
Ustilago bothriochloae Ustilago compacta
Ustilago bothriochloae-intermediae Ustilago concelata
Ustilago bouriqueti Ustilago condigna
Ustilago braziliensis Ustilago consimilis
Ustilago brisae Ustilago constantineanui
Ustilago bromi-arvensis Ustilago controversa
Ustilago bromi-erecti Ustilago conventere-sexualis
Ustilago bromi-mallis Ustilago cordai
Ustilago bromina Ustilago corlarderiae var. araucana
Ustilago bromivora f. brachypodii Ustilago coronariaw
Ustilago bromivora var. microspora Ustilago coronata
Ustilago bullata f. brachypodii-distachyi Ustilago courtoisii
Ustilago bullata var. bonariesis Ustilago crus-galli var. minor
Ustilago bullata var. macrospora Ustilago cryptica
Ustilago bungeana Ustilago curta
Ustilago calanagrostidis Ustilago custanaica
Ustilago calanagrostidis var. scrobiculata Ustilago cynodontis
Ustilago calanagrostidis var. typica Ustilago cynodontis
Ustilago cardamines Ustilago cyperi-lucidi
Ustilago cariciphila Ustilago davisii
Ustilago caricis-wallichianae Ustilago deccanii
Ustilago carnea Ustilago decipiens
Ustilago catherimae Ustilago deformitis
Ustilago caulicola Ustilago dehiscens
Ustilago cenrtodomis Ustilago delicata
Ustilago ceparum Ustilago deyeuxiae
Ustilago cephalariae Ustilago dianthorum
Ustilago chacoensis Ustilago distichlidis
Ustilago chloridii Ustilago dubiosa
Ustilago chloridionis Ustilago dumosa
Ustilago chrysopoganis Ustilago earlei
Ustilago chubulensis Ustilago echinochloae
Ustilago cichorii Ustilago ehrhartana
Ustilago cilmodis Ustilago eleocharidis
Ustilago clelandii Ustilago eleusines Ustilago elymicola Ustilago hieronymi var. minor
Ustilago elytrigiae Ustilago hilariicola
Ustilago enneapogonis Ustilago hilubii
Ustilago epicampida Ustilago himalensis
Ustilago eragrostidis-japanicana Ustilago histortarum var. marginalis
Ustilago eriocauli Ustilago hitchcockiana
Ustilago eήochloae Ustilago holci-avanacei
Ustilago euphorbiae Ustilago hordei
Ustilago fagopyri Ustilago hordei f. sp. avenae
Ustilago festucae Ustilago hsuii
Ustilago festucorum Ustilago hyalino-bipolaris
Ustilago filamenticola Ustilago hydropiperis
Ustilago fingerhuthiae Ustilago hyparrheniae
Ustilago flectens Ustilago hypodyies f. congoensis
Ustilago flonersii Ustilago hypodytes f. sporaboli
Ustilago foliorum Ustilago hypodytes var. agrestis
Ustilago formosana Ustilago idonea
Ustilago fueguina Ustilago imperatue
Ustilago gageae Ustilago induia
Ustilago garcesi Ustilago inouyei
Ustilago gardneri Ustilago intercedens
Ustilago gausenii Ustilago iranica
Ustilago gayazana Ustilago isachnes
Ustilago gigantispora Ustilago ischaemi-akoensis
Ustilago glyceriae Ustilago ischaemi-anthephoroides
Ustilago gregaria Ustilago ixiolirii
Ustilago grossheimii Ustilago ixophori
Ustilago gunnerae Ustilago jacksonii
Ustilago haesendocki var. chloraphorae Ustilago jacksonii var. vintonesis
Ustilago haesendocki var. vargasii Ustilago jaczevskyana
Ustilago halophiloides Ustilago jaczevskyana van. typica
Ustilago haynalodiae Ustilago jaczevskyana var. sibirica
Ustilago heleochloae Ustilago jagdishwari
Ustilago helictotrichi Ustilago jamalainentii
Ustilago herteri var. Bicolor Ustilago jehudana
Ustilago herteri var. vargasii Ustilago johnstonii
Ustilago hierochloae-adoratae Ustilago kairamoi
Ustilago hieronymi var. insularis Ustilago kasuchstemica Ustilago kenjiana Ustilago monermae
Ustilago kweichowensis Ustilago morinae
Ustilago kylingae Ustilago morobiana
Ustilago lacjrymae-jobi Ustilago mrucata
Ustilago lepyrodiclidis Ustilago muda
Ustilago lidii Ustilago muehlenbergiae var. lucumanensis
Ustilago liebenbergii Ustilago muscaribotryoidis
Ustilago linden Ustilago nagarnyi
Ustilago linearis Ustilago nannfeldtii
Ustilago Ii rove Ustilago nauda var. hordei
Ustilago loliicola Ustilago nelsoniana
Ustilago longiflora Ustilago nepalensis
Ustilago longiseti Ustilago neyraudiae
Ustilago longissima var. dubiosa Ustilago nigra
Ustilago longissima var. paludificans Ustilago nivalis
Ustilago longissima var. typica Ustilago nuda
Ustilago lupini Ustilago nuda
Ustilago lychnidis-dioicae Ustilago nuda var.tritici
Ustilago lycoperdiformis Ustilago nyassae
Ustilago lyginiae Ustilago okudairae
Ustilago machili Ustilago olida
Ustilago machringiae Ustilago olivacea var. macrospora
Ustilago magalaspora Ustilago onopordi
Ustilago magellanica Ustilago onumae
Ustilago mariscana Ustilago opiziicola
Ustilago maydis Ustilago oplismeni
Ustilago melicae Ustilago orientalis
Ustilago merxmuellerana Ustilago otophora
Ustilago mesatlantica Ustilago ovariicola
Ustilago michnoana Ustilago overcemii
Ustilago microspora Ustilago pamirica
Ustilago microspora var. paspalicola Ustilago panici-geminati
Ustilago microstegii Ustilago panjabensis
Ustilago microthelis Ustilago pappophori
Ustilago milli Ustilago pappophori var. magdalensis
Ustilago mobtagnei var. minor Ustilago parasnothii
Ustilago modesta Ustilago parodii
Ustilago moenchiae-manticae Ustilago parvula Ustilago paspalidiicola Ustilago sabouriana
Ustilago patagonica Ustilago salviae
Ustilago penniseti var. verruculosa Ustilago sanctae-catharinae
Ustilago perrara Ustilago scaura
Ustilago persicariae Ustilago scillae
Ustilago petrakii Ustilago scitaminea
Ustilago phalaridis Ustilago scitaminea var. sacchar-officinorum
Ustilago phlei Ustilago scleranthi
Ustilago phlei-protensis Ustilago scrobiculata
Ustilago phragmites Ustilago scutulata
Ustilago picacea Ustilago secalis var. elymi
Ustilago pimprina Ustilago seitaminea var. sacchari-barberi
Ustilago piperi (var.) rosulata Ustilago semenoviana
Ustilago poae Ustilago serena
Ustilago poae-bulbosae Ustilago serpens
Ustilago poae-nemoralis Ustilago sesleriae
Ustilago polygoni-alati Ustilago setariae-mambassanae
Ustilago polygoni-alpini Ustilago shastensis
Ustilago polygoni-punctari Ustilago shimadae
Ustilago polygon i-serrulati Ustilago silenes-inflatae
Ustilago polytocae Ustilago silenes-nutantis
Ustilago polytocae-harbatas Ustilago sinkiangensis
Ustilago pospelovii Ustilago sitanil
Ustilago prostata Ustilago sleuneri
Ustilago pseudohieronymi Ustilago sonoriana
Ustilago puehlaensis Ustilago sorghi-stipoidei
Ustilago puellaris Ustilago spadicea
Ustilago pulvertulensa Ustilago sparoboli-indici
Ustilago raciborskiana Ustilago sparti
Ustilago radians Ustilago speculariae
Ustilago ravida Ustilago spegazzinii
Ustilago rechingeri Ustilago spegazzinii var. agrestis
Ustilago reticulara Ustilago spermophora var. orientalis
Ustilago reticulispora Ustilago spermophoroides
Ustilago rhei Ustilago spinulosa
Ustilago rhynchelytri Ustilago sporoboli-trenuli
Ustilago ruandenis Ustilago stellariae
Ustilago ruberculata Ustilago sterilis Ustilago stewartli Ustilago turcomanica
Ustilago stipae Ustilago turcomanica var. prostata
Ustilago striaeformis f. phlei Ustilago turcomanica var. typica
Ustilago striaeformis f. poa... Ustilago ugamica
Ustilago striaeformis f. poae-pratensis Ustilago ugandensis var. macrospora
Ustilago striiformis f. hierochloes-odoratae Ustilago underwoodii
Ustilago striiformis var. agrostidis Ustilago urginede
Ustilago striiformis var. dactylidis Ustilago urochloana
Ustilago striiformis var. hold Ustilago ustilaginea
Ustilago striiformis var. phlei Ustilago ustriculosa var. cordai
Ustilago striiformis var. poae Ustilago ustriculosa var. reticulata
Ustilago sumnevicziana Ustilago valentula
Ustilago superha Ustilago vavilori
Ustilago sydowiana Ustilago verecunda
Ustilago symbiotica Ustilago verruculosa
Ustilago taenia Ustilago versatilis
Ustilago taiana Ustilago vetiveriae
Ustilago tanakue Ustilago viola ceo-irregularis
Ustilago tenuispora Ustilago violaceu var. stellariae
Ustilago thaxteri Ustilago violaceuverrucosa
Ustilago tinontiae Ustilago williamsii
Ustilago togata Ustilago wynaadensis
Ustilago tournenxii Ustilago zambettakisii
Ustilago tovarae Ustilago zernae
Ustilago trachophora var. pacifica Venturia inaequalis
Ustilago trachyniae Xanthomonas campestris
Ustilago trachypogonis Xanthomonas oryzae
Ustilago tragana
Ustilago tragi
Ustilago tragica
Ustilago tragi-racemosi
Ustilago trichoneurana
Ustilago trichophora var. crus-galli
Ustilago trichophora var. panici-frumentacei
Ustilago triseti
Ustilago tritici forma specialis
Ustilago tucumariensis
Ustilago tumeformis The plant health compositions may be suitable for controlling the following phytopatho- genic insects:
insects from the order of the lepidopterans {Lepidoptera), for example Agrotis ypsilon, Agrotis segetum, Alabama argillacea, Anticarsia gemmatalis, Argyresthia conjugella, Autographa gamma, Bupalus piniarius, Cacoecia murinana, Capua reticulana, Cheima- tobia brumata, Choristoneura fumiferana, Choristoneura occidentalis, Cirphis unipuncta, Cydia pomonella, Dendrolimus pini, Diaphania nitidalis, Diatraea grandi- osella, Earias insulana, Elasmopalpus lignosellus, Eupoecilia ambiguella, Evetria bou- liana, Feltia subterranea, Galleria mellonella, Grapholitha funebrana, Grapholitha mo- lesta, Heliothis armigera, Heliothis virescens, Heliothis zea, HeIIuIa undalis, Hibernia defoliaria, Hyphantria cunea, Hyponomeuta malinellus, Keiferia lycopersicella, Lamb- dina fiscellaria, Laphygma exigua, Leucoptera coffeella, Leucoptera scitella, Lithocol- letis blancardella, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Lymantria monacha, Lyonetia clerkella, Malacosoma neustria, Mamestra brassicae, Orgyia pseu- dotsugata, Ostrinia nubilalis, Panolis flammea, Pectinophora gossypiella, Peridroma saucia, Phalera bucephala, Phthorimaea operculella, Phyllocnistis citrella, Pieris brassicae, Plathypena scabra, Plutella xylostella, Pseudoplusia includens, Rhyacionia frus- trana, Scrobipalpula absoluta, Sitotroga cerealella, Sparganothis pilleriana, Spodoptera frugiperda, Spodoptera littoralis, Spodoptera litura, Thaumatopoea pityocampa, Tortrix viridana, Trichoplusia ni and Zeiraphera canadensis,
beetles {Coleoptera), for example Agrilus sinuatus, Agriotes lineatus, Agriotes obscu- rus, Amphimallus solstitialis, Anisandrus dispar, Anthonomus grandis, Anthonomus pomorum, Aphthona euphoridae, Athous haemorrhoidalis, Atomaria linearis, Blasto- phagus piniperda, Blitophaga undata, Bruchus rufimanus, Bruchus pisorum, Bruchus lentis, Byctiscus betulae, Cassida nebulosa, Cerotoma trifurcata, Cetonia aurata, Ceuthorrhynchus assimilis, Ceuthorrhynchus napi, Chaetocnema tibialis, Conoderus vespertinus, Crioceris asparagi, Ctenicera ssp., Diabrotica longicornis, Diabrotica semipunctata, Diabrotica 12-punctata Diabrotica speciosa, Diabrotica virgifera, Epila- chna varivestis, Epitrix hirtipennis, Eutinobothrus brasiliensis, Hylobius abietis, Hypera brunneipennis, Hypera postica, lps typographus, Lema bilineata, Lema melanopus, Leptinotarsa decemlineata, Limonius californicus, Lissorhoptrus oryzophilus, Melanotus communis, Meligethes aeneus, Melolontha hippocastani, Melolontha melolontha, Oulema oryzae, Ortiorrhynchus sulcatus, Otiorrhynchus ovatus, Phaedon cochleariae, Phyllobius pyri, Phyllotreta chrysocephala, Phyllophaga sp., Phyllopertha horticola, Phyllotreta nemorum, Phyllotreta striolata, Popillia japonica, Sitona lineatus and Sito- philus granaria, flies, mosquitoes (Diptera), e.g. Aedes aegypti, Aedes albopictus, Aedes vexans, An- astrepha ludens, Anopheles maculipennis, Anopheles crucians, Anopheles albimanus, Anopheles gambiae, Anopheles freeborni, Anopheles leucosphyrus, Anopheles minimus, Anopheles quadrimaculatus, Calliphora vicina, Ceratitis capitata, Chrysomya bezziana, Chrysomya hominivorax, Chrysomya macellaria, Chrysops discalis, Chrysops silacea, Chrysops atlanticus, Cochliomyia hominivorax, Contarinia sorghicola Cordylobia anthropophaga, Culicoides furens, Culex pipiens, Culex nigripalpus, Culex quinquefasciatus, Culex tarsalis, Culiseta inornata, Culiseta melanura, Dacus cucurbi- tae, Dacus oleae, Dasineura brassicae, Delia antique, Delia coarctata, Delia platura, Delia radicum, Dermatobia hominis, Fannia canicularis, Geomyza Tripunctata, Gaster- ophilus intestinalis, Glossina morsitans, Glossina palpalis, Glossina fuscipes, Glossina tachinoides, Haematobia irritans, Haplodiplosis equestris, Hippelates spp., Hylemyia platura, Hypoderma lineata, Leptoconops torrens, Liriomyza sativae, Liriomyza trifolii, Lucilia caprina, Lucilia cuprina, Lucilia sericata, Lycoria pectoralis, Mansonia titillanus, Mayetiola destructor, Musca domestica, Muscina stabulans, Oestrus ovis, Opomyza florum, Oscinella frit, Pegomya hysocyami, Phorbia antiqua, Phorbia brassicae, Phorbia coarctata, Phlebotomus argentipes, Psorophora columbiae, Psila rosae, Psoro- phora discolor, Prosimulium mixtum, Rhagoletis cerasi, Rhagoletis pomonella, Sar- cophaga haemorrhoidalis, Sarcophaga sp., Simulium vittatum, Stomoxys calcitrans, Tabanus bovinus, Tabanus atratus, Tabanus lineola, and Tabanus similis, Tipula ol- eracea, and Tipula paludosa
thrips {Thysanoptera), e.g. Dichromothrips corbetti, Dichromothrips ssp , Frankliniella fusca, Frankliniella occidentalis, Frankliniella tritici, Scirtothrips citri, Thrips oryzae, Thrips palmi and Thrips tabaci,
termites (Isoptera), e.g. Calotermes flavicollis, Leucotermes flavipes, Heterotermes aureus, Reticulitermes flavipes, Reticulitermes virginicus, Reticulitermes lucifugus, Termes natalensis, and Coptotermes formosanus,
cockroaches (Blattaria - Blattodea), e.g. Blattella germanica, Blattella asahinae, Peri- planeta americana, Periplaneta japonica, Periplaneta brunnea, Periplaneta fuligginosa, Periplaneta australasiae, and Blatta orientalis,
true bugs {Hemiptera), e.g. Acrosternum hilare, Blissus leucopterus, Cyrtopeltis nota- tus, Dysdercus cingulatus, Dysdercus intermedius, Eurygaster integriceps, Euschistus impictiventris, Leptoglossus phyllopus, Lygus lineolaris, Lygus pratensis, Nezara viridu- Ia, Piesma quadrata, Solubea insularis , Thyanta perditor, Acyrthosiphon onobrychis, Adelges lands, Aphidula nasturtii, Aphis fabae, Aphis forbesi, Aphis pomi, Aphis gos- sypii, Aphis grossulariae, Aphis schneideri, Aphis spiraecola, Aphis sambuci, Acyrtho- siphon pisum, Aulacorthum solani, Bemisia argentifolii, Brachycaudus cardui, Bra- chycaudus helichrysi, Brachycaudus persicae, Brachycaudus prunicola, Brevicoryne brassicae, Capitophorus horni, Cerosipha gossypii, Chaetosiphon fragaefolii, Cryptom- yzus ribis, Dreyfusia nordmannianae, Dreyfusia piceae, Dysaphis radicola, Dysaulacor- thum pseudosolani, Dysaphis plantaginea, Dysaphis pyri, Empoasca fabae, Hyalopte- rus pruni, Hyperomyzus lactucae, Macrosiphum avenae, Macrosiphum euphorbiae, Macrosiphon rosae, Megoura viciae, Melanaphis pyrarius, Metopolophium dirhodum, Myzus persicae, Myzus ascalonicus, Myzus cerasi, Myzus varians, Nasonovia ribis- nigri, Nilaparvata lugens, Pemphigus bursarius, Perkinsiella saccharicida, Phorodon humuli, Psylla mali, Psylla piri, Rhopalomyzus ascalonicus, Rhopalosiphum maidis, Rhopalosiphum padi, Rhopalosiphum insertum, Sappaphis mala, Sappaphis mali, Schizaphis graminum, Schizoneura lanuginosa, Sitobion avenae, Trialeurodes vapora- riorum, Toxoptera aurantiiand, Viteus vitifolii, Cimex lectularius, Cimex hemipterus, Reduvius senilis, Triatoma spp., and Arilus critatus.
ants, bees, wasps, sawflies (Hymenoptera), e.g. Athalia rosae, Atta cephalotes, Atta capiguara, Atta cephalotes, Atta laevigata, Atta robusta, Atta sexdens, Atta texana, Crematogaster spp., Hoplocampa minuta, Hoplocampa testudinea, Monomorium pha- raonis, Solenopsis geminata, Solenopsis invicta, Solenopsis richteri, Solenopsis xyloni, Pogonomyrmex barbatus, Pogonomyrmex californicus, Pheidole megacephala, Da- symutilla occidentalis, Bombus spp. Vespula squamosa, Paravespula vulgaris, Parave- spula pennsylvanica, Paravespula germanica, Dolichovespula maculata, Vespa crabro, Polistes rubiginosa, Camponotus floridanus, and Linepithema humile,
crickets, grasshoppers, locusts (Orthoptera), e.g. Acheta domestica, Gryllotalpa gryllo- talpa, Locusta migratoria, Melanoplus bivittatus, Melanoplus femurrubrum, Melanoplus mexicanus, Melanoplus sanguinipes, Melanoplus spretus, Nomadacris septemfasciata, Schistocerca americana, Schistocerca gregaria, Dociostaurus maroccanus, Tachycines asynamorus, Oedaleus senegalensis, Zonozerus variegatus, Hieroglyphus daganensis, Kraussaria angulifera, Calliptamus italicus, Chortoicetes terminifera, and Locustana pardalina,
Arachnoidea, such as arachnids (Acarina), e.g. of the families Argasidae, Ixodidae and Sarcoptidae, such as Amblyomma americanum, Amblyomma variegatum, Ambryomma maculatum, Argas persicus, Boophilus annulatus, Boophilus decoloratus, Boophilus microplus, Dermacentor silvarum, Dermacentor andersoni, Dermacentor variabilis, Hyalomma truncatum, Ixodes ricinus, Ixodes rubicundus, Ixodes scapularis, Ixodes holocyclus, Ixodes pacificus, Ornithodorus moubata, Ornithodorus hermsi, Ornithodo- rus turicata, Ornithonyssus bacoti, Otobius megnini, Dermanyssus gallinae, Psoroptes ovis, Rhipicephalus sanguineus, Rhipicephalus appendiculatus, Rhipicephalus evertsi, Sarcoptes scabiei, and Eriophyidae spp. such as Aculus schlechtendali, Phyllocoptrata oleivora and Eriophyes sheldoni; Tarsonemidae spp. such as Phytonemus pallidus and Polyphagotarsonemus latus; Tenuipalpidae spp. such as Brevipalpus phoenicis; Te- tranychidae spp. such as Tetranychus cinnabarinus, Tetranychus kanzawai, Tetran- ychus pacificus, Tetranychus telarius and Tetranychus urticae, Panonychus ulmi, Pa- nonychus citri, and Oligonychus pratensis; Araneida, e.g. Latrodectus mactans, and Loxosceles reclusa,
fleas (Siphonaptera), e.g. Ctenocephalides felis, Ctenocephalides canis, Xenopsylla cheopis, Pulex irritans, Tunga penetrans, and Nosopsyllus fasciatus,
silverfish, firebrat (Thysanura), e.g. Lepisma saccharina and Thermobia domestica,
centipedes (Chilopoda), e.g. Scutigera coleoptrata,
millipedes (Diplopoda), e.g. Narceus spp.,
earwigs (DermapteraJ, e.g. forficula auricularia,
lice (Phthiraptera), e.g. Pediculus humanus capitis, Pediculus humanus corporis, Pthi- rus pubis, Haematopinus eurysternus, Haematopinus suis, Linognathus vituli, Bovicola bovis, Menopon gallinae, Menacanthus stramineus and Solenopotes capillatus.
h) Determination of plant health activity
The plant health acitivity of the combinations of the invention may be determined in a manner known per se.
For example, the fungicidal action of components 1 ) and 2) and of the compositions according to the invention may be demonstrated in the following manner
Components 1 ) and 2), separately or jointly, were prepared as a stock solution comprising 25 mg of active compound which was made up to 10 ml using a mixture of acetone and/or DMSO and the emulsifier Uniperol® EL (wetting agent having an emulsifying and dispersing action based on ethoxylated alkylphenols) in a ratio by volume of solvent/emulsifier of 99:1. The mixture was then made up to 100 ml with water. This stock solution was diluted with the solvent/emulsifier/water mixture described to give the concentration of active compound stated below. The visually determined percentages of infected leaf areas were converted into efficacies in % of the untreated control:
The efficacy (E) is calculated as follows using Abbot's formula: E = (1 - α/β) - 100
α corresponds to the fungicidal infection of the treated plants in % and β corresponds to the fungicidal infection of the untreated (control) plants in %
An efficacy of 0 means that the infection level of the treated plants corresponds to that of the untreated control plants; an efficacy of 100 means that the treated plants were not infected.
The expected efficacies of active compound combinations were determined using Colby's formula (Colby, S. R. "Calculating synergistic and antagonistic responses of herbicide combinations", Weeds, 15, pp. 20-22, 1967) and compared with the observed efficacies.
Colby's formula: E = x + y - x-y/100
E expected efficacy, expressed in % of the untreated control, when using the mixture of the active compounds A and B at the concentrations a and b x efficacy, expressed in % of the untreated control, when using the active compound A at the concentration a y efficacy, expressed in % of the untreated control, when using the active compound B at the concentration b

Claims

Claims
1. A plant health composition, comprising the following components 1) and 2):
1 ) comprising at least one viable microorganism (I) having plant health activity and
2) comprising at least one chemical compound (II) having plant health and/or crop protection activity.
2. The plant health composition of claim 1 , wherein said viable beneficial microor- ganism (I) is a viable plant protective or plant performance or nutrition improving microorganism (I).
3. The plant health composition of claim 1 or 2, containing as component 1 ) a particulate formulation of at least beneficial microorganism (I) and a particulate carrier substance.
4. The plant health composition of claim 3, wherein said particulate formulation comprises a water insoluble, water-absorbent carrier substance, wherein said carrier substance is present in an amount of at least about 40% by the total weight of the formulation and at least one microorganism (I) mixed with said carrier.
5. The plant health composition of claim 4, wherein said water insoluble, water- absorbent carrier substance is selected from the group consisting of porous beads or powders, silica, ground agricultural products, porous wood products, cellulose, cyclodextrins, and combinations thereof.
6. The plant health composition of one of the claims 3 to 5, wherein said particulate formulation is coated.
7. The plant health composition of one of the preceding claims, wherein the microorganism (I) is selected from the group consisting of bacteria, in any developmental stage thereof, and combinations of at least two said microorganisms from the same or different group or developmental stage.
8. The plant health composition of claim 7, wherein the microorganism (I) is selected from the family of Rhizobiaceae, as for example of the genus Rhizobium spp., and Bradyrhizobium spp..
9. The plant health composition of one of the preceding claims, wherein the at least one chemical compound (II) is selected from pesticidal compounds, in particular fungicidal and insecticidal compounds.
10. The plant health composition according to claim 9, wherein the chemical compound (II) is selected from the
Insecticides A: acetamiprid, alpha-cypermethrin, clothianidin, fipronil, imidaclo- prid, spinosad, tefluthrin, thiamethoxam, metaflumizon; and/or
Fungicides B: metalaxyl, pyrimethanil, epoxiconazole, fluquiconazole, flutriafol, hymexazole, imazalil, metconazole, prochloraz, prothioconazole, tebuconazole, triticonazole, iprodion, metiram, thiram, boscalid, carbendazim, silthiofam, fludi- oxonil, azoxystrobin, , kresoxim-methyl, orysastrobin, pyraclostrobin triflox- ystrobin, thiophante methyl, ethaboxam.
1 1. The plant health composition according to claim 10, wherein the component 1 ) is selected from EXCALIBRE ™ and EXCALIBRE QR™.
12. The plant health composition according to one of the preceding claims, comprising the components 1) and 2) in a weight ratio of from 100:1 to 1 :100.
13. A method for controlling phytopathogenic harmful pests, wherein the pests, their habitat or the plants to be protected against pest attack, the locus where the plant is growing or is intended to grow and/or the plant propagules are/is treated with an effective amount of a component 1 ) and a component 2) according to any one of the claims 1 to 11.
14. A method for improving plant health and/or crop protection, wherein the plants, the locus where the plant is growing or is intended to grow and/or the plant propagules are/is treated with an effective amount of a component 1 ) and a component 2) according to any one of the claims 1 to 12.
15. The method according to claim 13 or 14, wherein components 1 ) and 2) are ap- plied simultaneously, that is jointly or separately, or in succession.
16. The use of components 1 ) and 2) according to any of claims 1 to 12 for preparing a plant health agent.
17. The use of components 1 ) and 2) according to any of claims 1 to 12 for treating transgenic plants or the seed thereof.
18. The method or use of one of the claims 13 to 17, wherein agricultural plants, sil- vicultural plants and ornamental plants.
19. The use or the method as claimed in claim 18, where the agricultural plant is selected from soybean, corn, wheat, triticale, barley, oats, rye, rape, millet, rice, sunflower, cotton, sugar beets, pome fruit, stone fruit, citrus, bananas, strawberries, blueberries, almonds, grapes, mango, papaya, peanuts, potatoes, tomatoes, peppers, cucurbits, cucumbers, melons, watermelons, garlic, onions, carrots, cabbage, beans, peas, lentils, alfalfa, trefoil, clovers, flax, elephant grass, grass, lettuce, sugar cane, tea, tobacco and coffee; each in natural or genetically modified form.
20. Kit-of-parts comprising a first component which contains at least one component 1 ) comprising at least one viable microorganism (I) which is as defined in any of claims 1 to 8 or 11 , and optionally at least one conventional auxiliary; and at least one component 2) which contains the least one component (II) which is as defined in any of claims 1 , 9 or 10, and optionally at least one conventional auxil- iary.
21. Plant seed, comprising a composition according to any of claims 1 to 12.
PCT/EP2008/0650282007-11-062008-11-05Plant health compositions comprising a beneficial microorganism and a pesticideWO2009060012A2 (en)

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