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WO2009052348A3 - Manipulation of beads in droplets - Google Patents

Manipulation of beads in dropletsDownload PDF

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Publication number
WO2009052348A3
WO2009052348A3PCT/US2008/080264US2008080264WWO2009052348A3WO 2009052348 A3WO2009052348 A3WO 2009052348A3US 2008080264 WUS2008080264 WUS 2008080264WWO 2009052348 A3WO2009052348 A3WO 2009052348A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
beads
actuator
droplets
manipulation
Prior art date
Application number
PCT/US2008/080264
Other languages
French (fr)
Other versions
WO2009052348A2 (en
Inventor
Ramakrishnan Sista
Vamsee Pamula
Vijay Srinivasan
Michael Pollack
Original Assignee
Advanced Liquid Logic Inc
Eckhardt Allen
Ramakrishnan Sista
Vamsee Pamula
Vijay Srinivasan
Michael Pollack
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc, Eckhardt Allen, Ramakrishnan Sista, Vamsee Pamula, Vijay Srinivasan, Michael PollackfiledCriticalAdvanced Liquid Logic Inc
Priority to EP08840456ApriorityCriticalpatent/EP2212683A4/en
Publication of WO2009052348A2publicationCriticalpatent/WO2009052348A2/en
Publication of WO2009052348A3publicationCriticalpatent/WO2009052348A3/en
Priority to US12/761,147prioritypatent/US8470606B2/en
Priority to US12/761,066prioritypatent/US8809068B2/en
Priority to US14/308,110prioritypatent/US9086345B2/en
Priority to US14/746,276prioritypatent/US9377455B2/en
Priority to US14/978,935prioritypatent/US9494498B2/en
Priority to US15/266,693prioritypatent/US10139403B2/en
Priority to US16/191,270prioritypatent/US10809254B2/en
Priority to US16/948,074prioritypatent/US11789015B2/en

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Abstract

The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnet field present at a portion of the plurality of droplet operations electrodes. A bead bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field. Other embodiments are also provided.
PCT/US2008/0802642006-04-182008-10-17Manipulation of beads in dropletsWO2009052348A2 (en)

Priority Applications (9)

Application NumberPriority DateFiling DateTitle
EP08840456AEP2212683A4 (en)2007-10-172008-10-17Manipulation of beads in droplets
US12/761,147US8470606B2 (en)2006-04-182010-04-15Manipulation of beads in droplets and methods for splitting droplets
US12/761,066US8809068B2 (en)2006-04-182010-04-15Manipulation of beads in droplets and methods for manipulating droplets
US14/308,110US9086345B2 (en)2006-04-182014-06-18Manipulation of beads in droplets and methods for manipulating droplets
US14/746,276US9377455B2 (en)2006-04-182015-06-22Manipulation of beads in droplets and methods for manipulating droplets
US14/978,935US9494498B2 (en)2006-04-182015-12-22Manipulation of beads in droplets and methods for manipulating droplets
US15/266,693US10139403B2 (en)2006-04-182016-09-15Manipulation of beads in droplets and methods for manipulating droplets
US16/191,270US10809254B2 (en)2006-04-182018-11-14Manipulation of beads in droplets and methods for manipulating droplets
US16/948,074US11789015B2 (en)2006-04-182020-09-01Manipulation of beads in droplets and methods for manipulating droplets

Applications Claiming Priority (2)

Application NumberPriority DateFiling DateTitle
US98078207P2007-10-172007-10-17
US60/980,7822007-10-17

Related Parent Applications (1)

Application NumberTitlePriority DateFiling Date
US11/639,531Continuation-In-PartUS8613889B2 (en)2006-04-132006-12-15Droplet-based washing

Related Child Applications (3)

Application NumberTitlePriority DateFiling Date
US12/761,066ContinuationUS8809068B2 (en)2006-04-182010-04-15Manipulation of beads in droplets and methods for manipulating droplets
US12/761,066Continuation-In-PartUS8809068B2 (en)2006-04-182010-04-15Manipulation of beads in droplets and methods for manipulating droplets
US12/761,147ContinuationUS8470606B2 (en)2006-04-182010-04-15Manipulation of beads in droplets and methods for splitting droplets

Publications (2)

Publication NumberPublication Date
WO2009052348A2 WO2009052348A2 (en)2009-04-23
WO2009052348A3true WO2009052348A3 (en)2009-07-30

Family

ID=40568074

Family Applications (1)

Application NumberTitlePriority DateFiling Date
PCT/US2008/080264WO2009052348A2 (en)2006-04-182008-10-17Manipulation of beads in droplets

Country Status (2)

CountryLink
EP (1)EP2212683A4 (en)
WO (1)WO2009052348A2 (en)

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US9184099B2 (en)2010-10-042015-11-10The Board Of Trustees Of The Leland Stanford Junior UniversityBiosensor devices, systems and methods therefor
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US9926596B2 (en)2011-05-272018-03-27Genapsys, Inc.Systems and methods for genetic and biological analysis
US8585973B2 (en)2011-05-272013-11-19The Board Of Trustees Of The Leland Stanford Junior UniversityNano-sensor array
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Also Published As

Publication numberPublication date
EP2212683A2 (en)2010-08-04
EP2212683A4 (en)2011-08-31
WO2009052348A2 (en)2009-04-23

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