RELATED APPLICATIONSThis is a continuation reissue of U.S. patent application Ser. No. 15/938,612, filed Mar. 28, 2018, now U.S. Pat. No. RE48,169, issued Aug. 25, 2020, which is a reissue of U.S. patent application Ser. No. 14/244,769, filed Apr. 3, 2014, now U.S. Pat. No. 9,925,236, issued Mar. 29, 2016, which is a continuation of U.S. patent application Ser. No. 11/767,562, filed Jun. 25, 2007, now U.S. Pat. No. 8,727,554, issued May 20, 2014. The contents of the aforementioned patent application is are hereby incorporated herein by reference in its entirety. Priority to the aforementioned application applications is hereby expressly claimed in accordance with 35 U.S.C. §120 and any other applicable statutes or laws.
FIELD OF THE INVENTIONThe present invention relates generally to aquariums and more particularly to aquariums having adjustable lighting systems, for example, for enhancing the display of fluorescent objects, such as fluorescent fish, contained within the aquarium.
BACKGROUND OF THE INVENTIONAquariums are typically comprised of a tank which can be filled with water, a system for maintaining the condition of the water (e.g. filter, aeration pump, heater), and ornamental features such as plants, gravel, rocks and curios. The tank may be of any shape such as rectangular tanks or round bowls, and the sides of the tank are typically transparent. The aquarium may also be provided with a lighting system.
Various fish tank lighting systems have been previously shown and described. For example, U.S. Pat. Nos. 3,836,765 and 5,089,940 describe lighting systems comprising a cover and a lighting fixture housed in the cover. The cover is configured to rest on the top of an aquarium tank.
U.S. Pat. No. 7,135,613, by Gong et al. (which is incorporated by reference herein in its entirety), discloses many different types of transgenic fluorescent fish and various methods of producing such fish. For instance, zebra fish transfected with green fluorescent protein (GFP) genes isolated from a jelly fish (Aqueoria Victoria) are described in detail. In addition, numerous modified mutants of GFP are disclosed, for example, various colors and mammalian optimized mutants are described. Fluorescence is the emission of light resulting from the absorption of excitation light. For example, GFP has a maximum excitation at a wavelength of 395 nm and emits green fluorescence at a wavelength (maximum) of 508 nm. The transgenic ornamental fish described in U.S. Pat. No. 7,135,613 (which is incorporated by reference herein in its entirety) are genetically engineered by introducing genes into the fish which express fluorescent proteins. By positioning the fluorescent gene under the control of a specific promoter, the fluorescent protein genes may be used to express the fluorescent proteins in specific tissues, such as in skin tissue, muscle tissue or bone tissue. Gong et al. disclose fish containing numerous different fluorescent proteins, including green fluorescent protein (GFP), enhanced green fluorescent protein (eGFP), yellow fluorescent protein (YFP), enhanced yellow fluorescent protein (eYFP), blue fluorescent protein (BFP), enhanced blue fluorescent protein (eBFP), cyan fluorescent protein (CFP) and enhanced cyan fluorescent protein (eCFP). There are also various colors of coral fluorescent proteins (available from Clontech, Inc.) which are suitable for creating transgenic ornamental fish. A summary of fluorescent protein genes is available on Table 1.
An aquarium for displaying fluorescent fish is described in U.S. patent application Ser. No. 10/627,176, filed Jul. 25, 2003, the disclosure of which is hereby incorporated by reference in its entirety.
All patents and patent applications referenced in this application are hereby incorporated by reference herein in their entirety.
SUMMARY OF THE INVENTIONThe aquarium and aquarium kit of the present invention is directed to enhancing the display of fluorescent ornamental plants, animals or other animate or inanimate objects (“fluorescent objects”) contained within the aquarium, such as wild-type fluorescent fish and transgenic fluorescent fish, under various ambient lighting conditions. The aquarium comprises a tank for containing a volume of water, at least two different light sources, and at least one control for controlling the operation of the light sources.
A first light source of the aquarium is configured to emit light at a first wavelength which enhances the display of the fluorescent objects under dark external lighting conditions. For instance, in a dimly lit or dark room, such as at night, the ambient light within the tank will be lit very little from the external light, and most of the light within the tank will emanate from the aquarium's light source(s). Under dark external lighting conditions, the display of the fluorescent objects will be enhanced by a first light source which causes the fluorescent objects to fluoresce, but which otherwise creates minimal visible ambient light within the tank. As an example, a black light emits ultra-violet light which is mostly outside the visible spectrum, but which causes many fluorescent materials to fluoresce. The result is that the fluorescent objects stand out brightly amidst the mostly dark ambient visible light within the tank, thereby enhancing the display of the fluorescent objects.
The aquarium further comprises a second light source configured to emit light at a second wavelength which enhances the display of the fluorescent objects under bright external lighting conditions. In daylight, or in a brightly lit room, the external lighting will create significant light within the tank. Accordingly, the ambient light within the tank will be relatively bright regardless whether the aquarium light source(s) provide any light within the tank. Thus, the amount of visible light provided within the tank by the aquarium light sources is mostly insignificant, as compared to the light created by the bright external lighting condition. In this situation, the display of the fluorescent objects is best enhanced by maximizing the intensity of the fluorescent excitation light source. Since a black light has a relatively low intensity (because most of the visible light is filtered out), a blue light or even a white light best enhances the display of the fluorescent objects under bright external lighting conditions.
In another aspect of the present invention, a viewing ratio is defined as the ratio of the intensity of the visible fluorescent light emitted by the fluorescent objects to the intensity of the visible ambient light within the tank. Thus, in another feature of the present invention, the first light source is configured to emit light at a first wavelength spectrum selected to obtain a viewing ratio under a dark external lighting condition of at least 75% of the highest possible viewing ratio for any achievable wavelength spectrum (also defined herein as the “percentage of the maximum viewing ratio”). For example, a laser light source emitting at the maximum excitation wavelength of the fluorescent object and which emits substantially no visible light, would provide for the highest possible viewing ratio. Similarly, the second light source may be configured to emit light at a second wavelength spectrum selected to obtain a viewing ratio of at least 75% of the maximum viewing ratio under a bright external lighting condition. Alternatively, the first and second light sources may provide for a viewing ratio of at least 50%, or at least 40%, or at least 25%, of the maximum viewing ratio for the respective external lighting condition.
The control for the light sources may a simple on/off switch for each light source, or one multi-positional switch that controls all light sources. Alternatively, the control may comprise a light sensor and the control may be configured to automatically, selectively control the light sources based at least in part on the lighting condition sensed by the light sensor. Another alternative would be to control the lighting source(s) through use of a timer, which would be set based on clock time (i.e. relative time), or a pre-determined number of hours for any particular setting. For example, without limitation a black light may stay on for exactly four hours, at which time a white light may come on, or a black light may stay on from 8 pm until 6 am, at which point a white light might come on. It should be understood that a timing device may control any light source(s) in a similar fashion.
In another feature of the present invention, the light source(s) may comprise an array of lights, such as an array of LED (light emitting diode) lights. Each array may be configured to primarily emit light of a certain desired wavelength spectrum. For example, one array of LEDs may emit blue light, while another array of LEDs emits ultraviolet light.
The ornamental fish may be a transgenic fish comprising one or more chimeric fluorescence genes which expresses one or more fluorescent proteins at a level sufficient such that the fish fluoresces upon exposure to the excitation light source.
In addition, the aquarium kit may comprise an ornamental fish which expresses one or more fluorescent protein genes at a level sufficient such that said fish fluoresces upon exposure to the excitation light source. The ornamental transgenic fish may comprise one or more fluorescent protein genes, including for example, without limitation, the following genes: GFP, eGFP, BFP, eBFP, YFP, eYFP, CFP, eCFP, reef coral fluorescent protein (“RCFP”), or any of the genes that code for expression of the fluorescent proteins listed in Table 1. It should be understood that each foregoing abbreviation identifies a fluorescent protein gene, which encodes a fluorescent protein. For example, “GFP” is used to identify the Green Fluorescent Protein Gene, which encodes green fluorescent protein. The fish may also comprise bio-luminescent proteins such as luciferase, where such bio-luminescent proteins would cause the fish to bio-luminesce. The ornamental transgenic fish may be any variety of aquatic animal, including without limitation, zebrafish, medaka, goldfish, carp, koi, tilapia, glassfish, catfish, angel fish, discus, eel, tetra, goby, gourami, guppy, Xiphophorus, hatchet fish, Molly fish, or pangasius. The kit may also comprise a fluorescent ornamental organism other than a fish, for example, including without limitation, fluorescent frogs, crabs, and shrimp.
BRIEF DESCRIPTION OF THE DRAWINGSFIG.1 is a perspective exploded view of an aquarium kit in accordance with an exemplary embodiment of the present invention.
FIG.2 is a chart of the excitation spectra and emission spectra for various fluorescent protein genes.
FIG.3 is an aquarium kit in accordance with the present invention.
DETAILED DESCRIPTION OF THE INVENTIONEmbodiments of the invention will now be described with reference to the accompanying Figures, wherein like numerals refer to like elements throughout. The terminology used in the description presented herein is not intended to be interpreted in any limited or restrictive manner, simply because it is being utilized in conjunction with a detailed description of certain specific embodiments of the invention. Furthermore, embodiments of the invention may include several novel features, no single one of which is solely responsible for its desirable attributes or which is essential to practicing the inventions herein described.
Referring toFIG.1, anaquarium10 according to one exemplary embodiment of the present invention is shown. Theaquarium10 is specially designed with adjustable lighting in order to enhance the display of fluorescent objects contained within the aquarium. The fluorescent objects may be fluorescent plants or fluorescent animals such as fluorescent fish, or fluorescent frogs.
Theaquarium10 comprises atank12, awater conditioning system14, acover16 and abase27. Thewater conditioning system14 comprises anaeration pump21 which is connected to a filter/diffuser25 by anair line tube23. Theaquarium10 is assembled by placing the filter/diffuser into the tank and affixing it to the side of the tank. Thecover16 is then placed onto the top of thetank12 with theair line tube23 extending through aslot31 in thecover16. Thebase27 has astorage drawer39 for storing fish food and other supplies, such as water conditioner.
Thecover16 has appropriately sized opening which allows the light to directly hit the water without being reflected by the cover. Alighting module19 is attached to theaquarium10 using abracket35 which attaches to theaquarium base27. Thehousing33 may be designed to cover all or just a portion of the open top of thetank12. Although thelighting module19 in the embodiment ofFIG.1 is shown mounted to the base27 through abracket35, it is contemplated that thelighting module19 may be attached to any part of thetank12, including the walls of thetank12, the top edge of thetank12, thecover16, or even inside thetank12 as part of a curio22 (seeFIG.3; for example, a translucent or transparent rock with an excitation light inside).
Thelighting module19 comprises three light sources, namely afirst light source32, a secondlight source34, and a thirdlight source36. It is to be understood that any number of multiple light sources may be utilized, including four, five, six or more light sources. Eachlight source32,34,36 comprises a circular array of eight LED lights, with each array located at a different radius of thelighting module19. Thefirst light source32 is located at the outer radius, the secondlight source34 is in the middle, and the thirdlight source36 is in the inner radius. Eachlight source32,34,36 is operably connected to anelectronic control38. Theelectronic control38 comprises threeswitches41 for controlling each of the threelight sources32,34,36.
Theelectronic control38 may be as simple as an on/off toggle switch for each light source as shown inFIG.1. Alternatively, theelectronic control38 may comprise a single multi-position switch (such as a rotating switch) such that each position of the switch turns on/off different light sources. For example, a first position of the toggle switch may turn on thefirst light source32, but turn off both the second and thirdlight sources34,36. In a second position, the toggle switch may turn off thefirst light source32, turn on the secondlight source34, and turn off the thirdlight source36, and so on. The toggle switch may also be configured to turn on any one of the light sources, or any combination of two or more of the light sources.
In another feature of the present invention, theelectronic control38 may comprise a light sensor (not shown). The light sensor is configured to detect the intensity of the external lighting conditions, for example, a dark external lighting condition, a moderate external lighting condition or a bright external lighting condition. Theelectronic control38 may then comprise an electronic circuit and components, as would be understood by one of skill in the art, to automatically, selectively control thelight sources32,34,36 based on the external lighting condition detected by the light sensor to enhance the display of fluorescent objects within theaquarium10, as described in further detail below. For example, theelectronic control38 may comprise a processor or other logic control, which receives an input signal from the light sensor, such as a voltage or current. The voltage or current can be related to the light intensity sensed by the light sensor. Based on this input, thecontrol38 selectively controls thelight sources32,34,36.
In another feature of the present invention, theelectronic control38 may comprise a timer that allows for the light source(s) to be turned on and off based on pre-determined chronological settings. For example, without limitation, a black light may stay on for exactly four hours, at which time the white light may come on, or a black light may stay on from 8 pm until 6 am (setting based on relative time), at which point a white light might come on. It should be understood that a timing device may control any light source(s) in a similar fashion.
The first, second and thirdlight sources32,24,36 are specifically configured to emit light at wavelength spectra selected to enhance the display of fluorescent objects within theaquarium10 under various external lighting conditions. Fluorescent materials fluoresce upon exposure to excitation light over a range (spectrum) of excitation wavelengths and similarly emit light over a spectrum of wavelengths. The excitation spectra and emission spectra for various fluorescent proteins are shown inFIG.2. Table 1 below lists the maximum excitation and emission wavelengths for various fluorescent proteins.
| TABLE 1 | 
|  | 
| Maximum Excitation and Emission Wavelengths | 
| for Fluorescent Proteins (“FP”) | 
|  |  | Excitation | Emission | 
|  | FP | max (nm) | max (nm) | 
|  | 
|  | AmCyan1 | 458 | 489 | 
|  | ZsGreen1 | 493 | 505 | 
|  | ZsYellow1 | 529 | 539 | 
|  | DsRed2 | 563 | 582 | 
|  | DsRed-Express | 557 | 579 | 
|  | AsRed2 | 576 | 592 | 
|  | HcRed1 | 588 | 618 | 
|  | mPlum | 590 | 649 | 
|  | mCherry | 587 | 610 | 
|  | tdTomato | 554 | 581 | 
|  | mStrawberry | 574 | 596 | 
|  | J-Red | 584 | 610 | 
|  | DsRed-monomer | 556 | 586 | 
|  | mOrange | 548 | 562 | 
|  | mKO | 548 | 559 | 
|  | MCitrine | 516 | 529 | 
|  | Venus | 515 | 528 | 
|  | Ypet | 517 | 530 | 
|  | EYFP | 514 | 527 | 
|  | Emerald | 487 | 509 | 
|  | EGFP | 488 | 507 | 
|  | CyPet | 435 | 477 | 
|  | mCFPm | 433 | 475 | 
|  | Cerulean | 433 | 475 | 
|  | T-Sapphire | 399 | 511 | 
|  | 
Referring to Table 1 andFIG.2, it can be seen that DsRed2 has a maximum excitation at a wavelength of 563 nm. This means that an excitation light that emits a high intensity of light at 563 nm will optimally cause this particular fluorescent protein to fluoresce. Therefore, because the wavelength at which DsRed2 has a maximum excitation is also in the visible range of light, the excitation light will be visible as well as the emitted fluorescent light thereby reducing the relative brightness of the emitted light. Generally, this will not be optimal for viewing these fluorescent fish, particularly under dark external lighting conditions. A chart of visible light is shown in Table 2 below.
| TABLE 2 | 
|  | 
| Chart of Colors of Visible Light | 
| Colors of Visible Light | 
|  | WAVELENGTH (nm) | PERCEIVED COLOR | 
|  | 
|  | ~410 | Violet | 
|  | ~440 | Blue | 
|  | ~500 | Green | 
|  | ~580 | Yellow | 
|  | ~650 | Red | 
|  | 
External light conditions can be quantified in terms of the light level or “illuminance” surrounding the aquarium. Illumenance is typically measured in foot candles (ftcd, fc) or lux in the metric SI system. A foot candle is actually one lumen of light density per square foot, one lux is one lumen per square meter. Some common, approximate, light levels for various indoor and outdoor conditions are listed in Table 3 below:
| TABLE 3 | 
|  | 
| Common Light Levels - Indoors and Outdoors | 
|  | Condition | Illumination (lux) | 
|  | 
|  | Full Daylight | 10,000 | 
|  | Overcast Day | 1000 | 
|  | Lighted Home | >150 | 
|  | LightedOffice | 500 | 
|  | Dark Indoor Room | <50 | 
|  | Moderately Lit Room | 100-150 | 
|  | 
With these concepts in mind, each of the lights sources32,34,36 may be configured to emit light at a desired wavelength spectra selected to enhance the display of fluorescent objects within theaquarium10 under various external lighting conditions. For example, the three light sources may be configured to enhance the display under the following three external lighting conditions: (a) a dark external lighting condition (defined herein to mean less than 50 lux); (b) a moderate external lighting condition (defined herein to mean 50-200 lux); or (c) a bright external lighting condition (defined herein to mean greater than 200 lux). This may entail configuring eachlight source32,34,36 to individually be lighted for a particular lighting condition, or a combination of two or more of thelight sources32,34,36 for a particular lighting condition. An example of dark external lighting condition would be a dimly lit or dark room, such as at night. An example of a moderate external lighting condition would include a room having enough functional light to read or watch TV, but less than daylight, and an example of a bright external lighting condition would be a room brightly lit from daylight or other light sources.
For instance, thelighting module19 may be configured to use only thefirst light source32 under dark external lighting conditions, only the secondlight source34 under moderate external lighting conditions, and only the thirdlight source36 under bright external lighting conditions. In this example then, thefirst light source32 is configured to emit light at a first wavelength spectrum (with a maximum peak emission wavelength different from the maximum peak emission wavelengths of both the second and third wavelength spectra) which causes the fluorescent object within theaquarium10 to fluoresce brightly, but which otherwise creates minimal visible ambient light within thetank12. A light which emits an excitation wavelength of the fluorescent material, but that is mostly out of the visible spectrum ultraviolet light is a proper choice. Thefirst light source32 may be a black light which emits mostly ultra-violet light which is mostly outside the visible spectrum, but which also will cause many fluorescent materials to fluoresce. For purposes of this application, the ultra-violet range is defined as light having a wavelength shorter than 410 nm. In the terms of the viewing ratio as defined above, thefirst light source32 is configured to emit light at a first wavelength spectrum which obtains a viewing ratio under a dark external lighting condition of at least 75%, or at least 50%, or at least 40%, or at least 25%, of the maximum viewing ratio.
Continuing with this example, with a moderate external lighting condition in mind, the secondlight source34 is configured to emit light at a second wavelength spectrum (with a maximum peak emission wavelength different from the maximum peak emission wavelengths of both the first and third wavelength spectra) which causes the fluorescent object within theaquarium10 to fluoresce brightly, and it is less important whether it otherwise creates visible ambient light within thetank12. Thus, the secondlight source34 may have a higher intensity near an excitation wavelength peak for the fluorescent object, even if that peak is in the visible range. An appropriate choice for the secondlight source34 may be a light which emits mostly blue light, a higher intensity light in the excitation wavelength range of many fluorescent materials. As used herein, the blue light range is considered to be light having a wavelength of about 460 nm-480 nm. In the terms of the viewing ratio, the secondlight source34 is configured to emit light at a second wavelength spectrum which obtains a viewing ratio under a moderate external lighting condition of at least 75%, or at least 50%, or at least 40%, or at least 25%, of the maximum viewing ratio.
Finally, with a bright external lighting condition in mind, the thirdlight source36 is configured to emit light at a third wavelength spectrum (with a maximum peak emission wavelength different from the maximum peak emission wavelength of both the first and second wavelength spectra) which causes the fluorescent object within theaquarium10 to fluoresce brightly, and it is even less important whether it otherwise creates visible ambient light within thetank12. The thirdlight source34 must have a high intensity at or near an excitation wavelength peak for the fluorescent object, even if that peak is in the visible range. Because it is mostly unimportant whether the thirdlight source36 emits visible light (because there is already bright ambient light), a light with a very high intensity at or near an excitation wavelength peak is required. An appropriate choice for the thirdlight source36 may be a light which emits mostly white light. In terms of the viewing ratio, the thirdlight source34 is configured to emit light at a third wavelength spectrum which obtains a viewing ratio under a bright external lighting condition of at least 75%, or at least 50%, or at least 40%, or at least 25%, of the maximum viewing ratio.
The operation of thelight sources32,34,36 is controlled by theelectronic control38, as described above. In this specific example, thecontrol38 is configured with threeindividual toggle switches44 to turn on/off each individuallight source32,34 or36, while the other light sources are left off. If a light sensor and automaticelectronic control38 are utilized, thecontrol38 turns on one of the threelight sources32,34, or36 depending on the external lighting condition detected by the light sensor.
The appropriate configuration of thelight sources32,34,36 may be chosen by knowing the excitation and emission spectrum of the particular fluorescent object(s) to be displayed in theaquarium10, the particular external lighting condition, and by making reference to the visible light spectrum.
Thelight sources32,34,36 may be any suitable type of light source, including without limitation, LED, incandescent light, fluorescent light, laser, xenon lamps, or a combination thereof. The lights may include filters in order to modify the wavelength spectra of the light source. Moreover, thelight sources32,3436, may be an array of individual lights, such as the arrays as shown inFIG.1. Thecontrol38 may be activated in any number of ways, including a manual switch, a push-button toggle, an infra-red remote, a radio frequency remote, an internal or external motion sensor, or a chemical or thermal activator. Thecontrol38 may also cause to thelight sources32,34,36 to operate in a variety of modes such as fading and transition modes, timer modes or light sensing modes.
In order to enhance the appearance of the transgenic fluorescent fish, theaquarium10 may further comprise light filters in or on the tank to block light outside the wavelength of the emission spectra of the particular fluorescent proteins in the transgenic fluorescent fish. The appearance of the fluorescent fish could also be enhanced using mirrors, one-way films, wavelength specific or polarizing films, specially angled walls of the tank or the use of special materials within the tank such as reflective mica rocks or such.
Thetank12 may have physical separators to maintain certain fish in different areas of thetank12 that are lit by the different light sources26.
Turning toFIG.3, anaquarium kit50 may comprise theaquarium10 described above, in addition to various ornamental features such asgravel18,plants20 andcurios22. Thegravel18,plants20 andcurios22 may also be fluorescent to augment the appearance of theaquarium kit50. For example, theplants20 may be transgenic or other specialty plants which are fluorescent. Thecurios22 can be small items such as a miniature treasure chest, marbles, artificial or actual marine objects like coral, rocks or sticks.
Theaquarium kit50 may also include the transgenic fluorescentornamental fish40 as shown inFIG.3. Transgenic fluorescent ornamental fish and the method of producing them are described in detail in U.S. Pat. No. 7,135,613 (which is incorporated by reference herein in its entirety), and therefore only a general description will be included herein. Generally, a transgenic fluorescent ornamental fish is produced by inserting a foreign gene which codes for a fluorescent protein into the genome of the host fish. Typically, the fluorescent gene is operatively linked to either an endogenous or exogenous promoter in the fish such that activation of the promoter causes expression of the fluorescent protein coded by the fluorescent gene. A chimeric fluorescent gene construct comprises a promoter operatively linked to a heterologous gene. For example, a chimeric fluorescent gene construct can comprise a promoter of a zebrafish operatively linked to a GFP gene or other fluorescent protein gene.
A stable transgenic ornamental fish line may be obtained by producing an ornamental transgenic fish comprising one or more chimeric fluorescence genes positioned under the control of a promoter such that the fish expresses one or more fluorescent proteins encoded by the fluorescence genes at a level sufficient that the fish fluoresces upon exposure to an excitation light source. The transgenic fish is then bred with a second fish to obtain offspring. Finally, a stable transgenic fish line that expresses the fluorescent proteins is selected from the offspring. The stable transgenic fish line may then be used to breed large numbers of ornamental fluorescent transgenic fish.