US8792156B1 - Laser illumination systems and methods for dual-excitation wavelength non-linear optical microscopy and micro-spectroscopy systems - Google Patents

Abstract

An illumination system is disclosed for providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy. The illumination system includes a laser system, an optical splitting means, a frequency shifting system, and a picosecond amplifier system. The laser system includes a laser for providing a first train of pulses at a center optical frequency ω₁. The optical splitting means divides the first train of pulses at the center optical frequency ω₁into two trains of pulses. The frequency shifting system shifts the optical frequency of one of the two trains of pulses to provide a frequency shifted train of pulses. The picosecond amplifier system amplifies the frequency shifted train of pulses to provide an amplified frequency-shifted train of pulses having a pulse duration of at least 0.5 picoseconds.

Description

SPONSORSHIP INFORMATION

This invention was made with government support under 1R01EB010244 awarded by National Institutes of Health. The government has certain rights in the invention.

BACKGROUND

The invention generally relates to label-free imaging systems, and relates in particular to non-linear optical microscopy and micro-spectroscopy imaging systems employing efficient dual frequency laser sources.

The development of confocal microscopy and genetically encodable fluorescent labels has transformed biological research. Labels however, may be perturbative of a sample, especially for imaging molecules that are smaller than typical fluorophores (e.g., metabolites or drugs) and that are not applicable for in vivo diagnostics due to toxicity and/or delivery concerns. Certain molecules or properties (e.g., strain or stress in material science samples) cannot be labeled at all, and photobleaching may be problematic for long term measurements.

Alternatively, vibrational spectroscopy may provide label-free chemical contrast based on intrinsic molecular properties of the sample. Yet, the traditional techniques, infrared absorption (IR) and spontaneous Raman, are limited, and IR microscopy suffers from low spatial resolution and limited imaging depth due to the long wavelength. Spontaneous Raman microscopy has slow imaging speed due to the weakness of the signal.

Micro-spectroscopy generally involves capturing a spectrum from a microscopic volume in a sample, while microscopy generally involves capturing an intensity value as well as scanning such that multiple intensity values are captured to form picture elements (pixels) of a microscopy image.

Infrared microscopy involves directly measuring the absorption of vibrationally excited states in a sample, but such infrared microscopy is generally limited by poor spatial resolution due to the long wavelength of infrared light, as well as by a low penetration depth due to a strong infrared light absorption by the water in biological samples.

Raman microscopy records the spontaneous inelastic Raman scattering upon a single (ultraviolet, visible or near infrared) continuous wave (CW) laser excitation. Raman microscopy has improved optical resolution and penetration depth as compared to infrared microscopy, but the sensitivity of Raman microscopy is rather poor because of the very low spontaneous Raman scattering efficiency (Raman scattering cross section is typically on the order of 10⁻³⁰cm²). This results in long averaging times per image, which limits the biomedical application of Raman microscopy.

Coherent Raman scattering (CRS) microscopy techniques, including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy allow signal amplification by up 100,000× compared to spontaneous Raman, enabling label-free imaging with high temporal (imaging speeds up to video-rate, i.e., 30 frames/s) and sub-micron spatial resolution. Due to the use of nonlinear excitation, CRS microscopy is intrinsically three-dimensional, allowing non-destructive optical sectioning of the sample. The excitation uses near-infrared light within the optical window of biological specimen, allowing imaging depths up to a few hundred microns.

CRS is also free of photobleaching, if electronic resonances are avoided; and auto-fluorescence does not interfere, because it is not coherently amplified. CRS imaging systems may be used in biology and material science research, such as studying lipid metabolism, optimizing drug formulation for trans-dermal delivery, and in biofuel production. Label-free microscopy is also being evaluated as a medical imaging modality for delineation of tumor margins in brain and breast cancer surgery and early detection of melanoma.

Despite the advantages of CRS, high instrument cost and the technical complexity limit its wider use and currently only a few laboratories can obtain high quality images. Providing laser sources for CRS is challenging not only due to the comparative cost of laser systems as compared to a complete conventional Raman system, but the illumination must be provided as two synchronized laser pulse-trains of picosecond pulse duration, with a tunable difference frequency to the precision of a typical Raman line width.

Modulation transfer microscopy and spectroscopy imaging systems such as stimulated Raman scattering (SRS), spectral excitation of stimulated Raman scattering (SRS Spectral), stimulated emission (SE), ground state depletion (GD), photo-thermal (PT), two-color two-photon absorption (TPA), and stimulated Brillouin scattering generally involve reliance on the non-linear interaction of two laser beams within a sample, and detection of a characteristic, such as gain or loss, of one of the excitation beams. This is in contrast to detecting a newly generated (new frequency) emission signal as is done, for example, in one-photon and two-photon excited fluorescence, spontaneous Raman scattering, coherent anti-Stokes Raman scattering (CARS), second harmonic generation, (SHG), sum frequency generation (SFG) and third harmonic generation (THG).

Such modulation transfer microscopy and micro-spectroscopy techniques require a detection scheme that provides for detection of a relatively small signal (e.g., a small gain and loss signal) on top of noisy lasers. This is generally achieved in accordance with various embodiments based on modulation transfer—by modulating a feature of one of the laser excitation beams and measuring the signal of interest with high sensitivity. In particular, the modulation transfers to the other excitation beam due to non-linear interaction within the sample, which facilitates detection of the signal of interest using a modulation sensitive detector. If the modulation frequency is chosen to be faster than the laser noise (e.g., greater than about 200 kHz), shot-noise limited sensitivity may be achieved. Such modulation schemes are readily compatible with beam-scanning microscopy and micro-endoscopy, video-rate imaging speeds, and multiplex excitation schemes.

An advantage of these non-linear optical imaging techniques as compared to fluorescence microscopy, is that they allow for specific image contrast based on intrinsic spectroscopic properties of the sample, rather than extrinsic fluorescent labeling or dye staining. This is particularly important for imaging of small molecules that can be perturbed by labeling and medical diagnostics because of a possible toxicity of the used dyes. In CARS and SRS, chemical contrast is derived from intrinsic molecular vibrations and in TPA, SE and GD microscopy from absorption properties of the molecules constituting the sample.

Common features of CARS and SRS include that each requires (1) pulsed laser beams with a pulse-width shorter than about 10 ps, i.e., a spectral bandwidth of at least about 30 GHz, and (2) two synchronized beams that are overlapped in time.

Modulation transfer techniques further require that a property (such as intensity, polarization or time delay) of one of the beams is modulated at a rate higher than 100 kHz allowing measurement the modulation transfer from this modulated beam to the second, originally un-modulated beam due to the nonlinear interaction in the sample

These different techniques have different laser wavelength requirements. For CARS an SRS, the difference between the two excitation frequencies (|ω₁−ω₂|) is selected to be resonant with a vibrational frequency of the sample. The specific wavelengths of the two excitation fields, therefore are not critical as long as the difference frequency is as desired. Such sources are typically chosen to be in the range of about 700 nm to about 1600 nm, for which biological samples are transparent. The tuning of the difference frequency to a vibrational frequency of the sample (about 200 cm⁻¹to about 4000 cm⁻¹) should be to a precision of at least about 2 nm.

Stimulated emission (SE) and ground state depletion (GD) microscopy involve tuning either ω₁or ω₂to be electronically resonant with the sample. With photo-thermal (PT) microscopy, either ω₁or ω₂is chosen to match the one or two photon electronic absorption frequency. With two-color two-photon absorption (TPA), the sum of ω₁and ω₂is chosen to be electronically resonant with the sample.

Many conventional laser systems for CARS and MTM techniques have involved the use of mode-locked solid state lasers in order to achieve the pulse width shorter than 10 ps as such pulse-width that cannot conventionally be achieved with an electrically driven laser systems. A particular challenge, is the requirement of overlaps the pulses in time precisely (synchronization), as timing jitter translates into severe noise of the signal if it is bigger than the pulse width (i.e., much smaller than the required 10 ps).

Certain conventional implementations of CARS microscopy involved using two Titanium Sapphire (Ti:Sa) lasers whose outputs were electronically locked to one another using feedback regarding the cavity length of one of the lasers. Both Ti:Sa lasers were continuously tunable from about 750 nm-1000 nm, which allowed imaging based on Raman frequencies in the entire spectral region from about 200 cm⁻¹-4000 cm⁻¹. Such systems however, suffered from timing jitter between the pulses, making long-term experiments impossible and limiting day-to-day stability of the system.

Later developed conventional system involved the use of optical parametric oscillators (OPO) for label-free microscopy that are intrinsically locked due to synchronously pumping the OPO with the same lasers that provides the first beam. Such OPO laser systems may also be pumped with made-locked fiber lasers. The pump laser is typically fixed at 1064 nm and the OPO output is tunable from 750 nm to 1000 nm, again allowing to image any Raman band. The long-term stability, complexity and price of such OPO laser systems however, remains a shortcoming of such systems. Moreover, dual frequency sources employing OPO laser systems typically include an adjustable translation stage that ensures that the resulting two trains of laser pulses are temporally overlapped. Such an adjustable translation stage adjusts the optical path of one of the pulse trains within a short range to ensure temporal synchronicity. Variations in temperature of the imaging system will also affect the path lengths and therefore synchronization.

Another approach to providing illumination systems for dual-excitation wavelength non-linear imaging systems has been based on time-lens lasers, which allow generation of pulses on demand with response to an electronic trigger signal. A Yb time-lens laser may be triggered by a Ti:Sa laser to provide to laser pulse trains for CRS with minimal timing jitter. One wavelength is fixed at 1040 nm and the other is tunable over the entire region of Raman spectra. Again however, the synchronization of the two oscillators is achieved electronically rather than by optical seeding.

Other approaches to providing laser pulse trains for CRS have been based on super-continuum generation (SC) in an optical fiber to generate a frequency shifted second train of pulses synchronized to the first train of pulses. Typically SC spectra are very broad, much more broad than the typical line-shape of Raman spectra and SC light sources for CRS are combined with spectral compression schemes to recover the chemical specificity of CRS. This may be achieved either by spectral focusing CRS or by spectral compression via sum frequency generation.

This permits the generation of the spectral brightness required for CRS imaging and allows for fast imaging speed with pixel dwell times as short as 4 μs. Because Raman SC spectra are very broad, SC light sources are broadly tunable (e.g., from 850 nm-1100 nm) and allow access Raman peaks across the full Raman spectrum. While relying on optical synchronization, this approach is different from the laser system disclosed herein in that the second pulse train is generated by super continuum generation in a nonlinear fiber rather than lasing; In the nonlinear fiber, the molecular population is unaffected similar to the parametric process in OPOs.

Ultrafast laser systems have also been disclosed based on seeding a Yb-doped amplified with a super-continuum generated from an Er-doped oscillator. Such systems however, only provide a single-color output, which is not suitable for CRS microscopy, which SRS requires the generation of two synchronized trains of picosecond pulses with narrow bandwidth (a few cm-1) and that are independently tunable over a wide spectral range 800-3300 cm-1. Further, they are intrinsically broadband due to the broad spectral range from the super-continuum fiber that directly seeds the amplifier.

There remains a need, therefore, for an efficient dual frequency laser system with reduced jitter for microscopy and micro-spectroscopy imaging systems.

SUMMARY

In accordance with an embodiment, the invention provides an illumination system for providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy. The illumination system includes a laser system, an optical splitting means, a frequency shifting system, and a picosecond amplifier system. The laser system includes a laser for providing a first train of pulses at a center optical frequency ω₁. The optical splitting means divides the first train of pulses at the center optical frequency ω₁into two trains of pulses. The frequency shifting system shifts the optical frequency of one of the two trains of pulses to provide a frequency shifted train of pulses. The picosecond amplifier system amplifies the frequency shifted train of pulses to provide an amplified frequency-shifted train of pulses having a pulse duration of at least 0.5 picoseconds. In accordance with further embodiments, the picosecond amplifier system may be a narrowband amplifier system or a chirped amplifier system.

In accordance with another embodiment, the invention provides an illumination system includes a laser system, an optical splitting means, a frequency shifting system, an amplifier system, and combining means. The amplifier system is for amplifying the frequency-shifted train of pulses to provide an amplified frequency-shifted train of pulses, and the combining means is for combining the amplified frequency-shifted train of pulses with a second of the two trains of laser pulses from the optical splitting means to provide the amplified frequency-shifted train of pulses and the second of the two trains of laser pulses from the optical splitting means as a collinear train of laser pulses for the dual-excitation wavelength illumination

In further embodiments, the illumination system is provided in a dual-excitation wavelength nonlinear Stimulated Raman Scattering microscopy or two-color two-photon microscopy system.

In accordance with a further embodiment, the invention provides a method of providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy. The method includes the steps of providing a first train of pulses at a center optical frequency ω₁using a laser oscillator; dividing the first train of pulses at the center optical frequency ω₁into a first split train of pulses and a second split train of pulses; shifting the optical frequency of the first split train of pulses to provide a frequency shifted train of pulses; and amplifying the frequency shifted train of pulses to provide a amplified frequency-shifted train of pulses having a pulse duration of at least 0.5 picoseconds.

BRIEF DESCRIPTION OF THE DRAWINGS

The following description may be further understood with reference to the accompanying drawings in which:

FIG. 1 shows an illustrative diagrammatic view of an illumination system and imaging system employing a dual-frequency source in accordance with an embodiment of the invention;

FIGS. 2A and 2B show illustrative diagrammatic views of narrowband excitation and output spectra in an SRS system in accordance with an embodiment of the present invention;

FIGS. 3A and 3B show illustrative graphical representations of estimated signal to noise ratios and required average power requirements respectively for pulse parameters in a CRS system;

FIG. 4 shows an illustrative diagrammatic view of a an illumination system in accordance with an embodiment of the invention;

FIG. 5 shows an illustrative diagrammatic view of a an laser system for use in the illumination system ofFIG. 4 in accordance with an embodiment of the invention;

FIG. 6 shows a illustrative diagrammatic view of a portion of the illumination system ofFIG. 4 employing a frequency shifting system in accordance with an embodiment of the invention;

FIG. 7 shows a illustrative diagrammatic view of a portion of the illumination system ofFIG. 4 employing a further frequency shifting system in accordance with another embodiment of the invention;

FIGS. 8-11 show illustrative diagrammatic views of a portion of the illumination system ofFIG. 4 employing further narrowband amplifier systems in accordance with further embodiments of the invention;

FIG. 12 shows an illustrative diagrammatic view of a portion of the illumination system ofFIG. 4 employing a plurality of amplifiers in accordance with an embodiment of the invention;

FIGS. 13-14 show illustrative diagrammatic views of a portion of the illumination system ofFIG. 4 employing a plurality of amplifiers in accordance with further embodiments of the invention;

FIGS. 15-16 show illustrative diagrammatic views of a portion of the illumination system ofFIG. 4 employing frequency doubling/tripling units in accordance with further embodiments of the invention;

FIGS. 17-19 show illustrative diagrammatic views of an illumination system in accordance with further embodiments of the invention employing various laser systems;

FIG. 20 shows an illustrative diagrammatic view of an illumination system in accordance with further embodiments of the invention employing a Erbium dopes fiber oscillator, as well as Erbium-doped and Ytterbium-doped power amplifiers;

FIGS. 21A-21B show illustrative graphical representations of tuning ranges for illumination sources in accordance with embodiments of the present invention employing different gain mediums;

FIG. 22 shows an illustrative graphical representation of the relationship between frequency and time, showing changes in the pulses over time and frequency that permits spectral focusing of the excitation illumination in accordance with an embodiment of the invention;

FIG. 23 shows an illustrative diagrammatic view of a portion of the illumination system ofFIG. 4 employing a chirped amplifier system in accordance with an embodiment of the invention;

FIG. 24 shows an illustrative diagrammatic view of a portion of the illumination system ofFIG. 4 employing a chirped amplifier system in accordance with another embodiment of the invention;

FIGS. 25-30 show illustrative diagrammatic views of a portion of the illumination system ofFIG. 4 employing a chirped amplifier system in accordance with further embodiments of the invention;

FIG. 31 shows an illustrative photographic representation of a CRS image of polystyrene beads obtained using an illumination system of the present invention;

FIG. 32 shows an illustrative graphical representation of gray value intensities along a cross-section of the photographic representation ofFIG. 31;

FIGS. 33A and 33B show illustrative photographic representations of CH2 vibrations (lipids) obtained using an illumination system of the present invention and of CH3-vibrations (proteins) obtained using an illumination system of the present invention; and

FIG. 34 shows an illustrative graphical representation of an image for sebaceous land in a subject.

The drawings are shown for illustrative purposes only.

DETAILED DESCRIPTION

The promise of label-free microscopy is that one may obtain rich, chemical specific contrast based on intrinsic properties of the sample. As each type of chemical bond has a specific stiffness (e.g., C═C is stiffer than C—C) and associated mass (e.g., C—C is heavier than C—H), it has a characteristic vibrational frequency Ω. Vibrational spectra of the sample, which consist of the vibrational frequencies of the molecule, provide a unique molecular fingerprint. Raman scattering is an elegant way to measure vibrational spectra with visible light. When a molecule is excited (de-excited), an incident photon is annihilated a new red-shifted (blue-shifted) photon is generated at the Stokes (anti-Stokes) frequency ω_S=ω_P−Ω (and ω_AS=ω_P+Ω) due to energy conservation. The emission spectra, σ(Ω)=σ(ω_P−ω_S), can be measured by dispersing the light on a spectrometer.

In a specific implementation, the invention provides an ultrafast dual-excitation wavelength laser source based on a fiber laser technology. Because light is guided within the optical fiber, misalignment is impossible. Existing fiber-lasers do not, however, reach the same performance level of free-space systems in CRS. An important realization is that the difference frequency of the two most common fiber gain media, erbium (Er) and ytterbium (Yb), coincides with the high-wavenumber region of Raman spectra, where most CRS microscopy is performed. The invention provides an all-fiber system based on optical synchronization of Er- and Yb-doped power amplifiers via super-continuum seeding and careful control the pulse properties for CRS microscopy.

FIG. 1 shows anillumination system10 in accordance with an embodiment of the invention together with amicroscopy imaging system12. Theillumination system10 includes a laser system14 (that provides two trains oflaser pulses16,18) and acombiner system20 that combines the two trains of laser pulses such that they are collinear and spatially and temporally overlapped. One of the trains oflaser pulses16 is at a center frequency ω₁(e.g., a Stokes frequency of about 1030 nm), and the other train oflaser pulses18 is at a different center frequency (e.g., 800 nm).

The combined trains oflaser pulses22 are directed via a scanhead24 (that scans in mutually orthogonal x and y directions), into amicroscope26 that includesoptics28 that direct and focus the combined trains of laser pulses into the focal volume, e.g., via amirror30. The illumination from the focal volume is directed by acondenser32 onto anoptical detector34. One of the trains oflaser pulses16 or18 (a first train of laser pulses) is modulated within the laser system responsive to amodulation signal36, and, at thedetector34 the modulated first beam (e.g., the Stokes beam) is blocked by anoptical filter38. Theoptical detector34, such as a photodiode, therefore measures the intensity of the other (second) beam (e.g., the pump beam) only.

The system may further include balanced detectors. In particular, anotheroptical detector40 may be employed with afilter41 such that thedetector40 only sees the original pump or Stokes beam that was not modulated. The difference between the outputs of thesedetectors34 and44 is provided by asubtraction unit43, which outputs the outputelectrical signal46. Adelay unit42 permits adjustment of the timing of the original second train of laser pulses. Anelectrical output signal46 is provided to asignal processor48.

The first train of laser pulses is modulated at modulation frequency f, by a modulation system that includes, for example, a modulator within the laser system as discussed in further detail below, acontroller50, and amodulation source52. The modulation source provides a common modulation control signal to thecontroller50 as well as to asignal processor48. The integrated intensity of substantially all frequency components of the second train of laser pulses from theoptical detector34 is provided to thesignal processor48, and the modulation (amplitude and/or phase) of the integrated intensity of substantially all the optical frequency components of the second train of laser pulses due to the non-linear interaction of the first and second trains of laser pulses in the focal volume is detected at the modulation frequency f to provide a pixel of an image to amicroscopy control computer54. Themicroscopy control computer54 is employed as an imaging system, and further provides user control of thescanhead24 as shown at56.

In a further embodiment, an epi-directed detection scheme may be employed wherein the illumination from the focal volume is received back through theoptics28 and passes through afilter62 to adetector60 that provides an electrical output signal to asubtraction unit68. Thesubtraction unit68 also receives an output signal from adetector58 via afilter66 that receives the original pump or Stokes beam that was not modulated. Again, adelay unit64 permits adjustment of the timing of the original second train of laser pulses. The use of the second detector and the subtraction unit provides that any laser background noise as well as any low frequency variations in the laser power, will be removed from the detected signal, (whether detected in the forward or epi direction).

Tuning control of the lasers output trains may also be provided using themicroscopy control computer54 that directs acontrol signal70 to thelaser system14 as shown. Such tuning control may control the frequency difference between the pump and Stokes beams to provide for tuning into different compositions in the sample. Coupled with the ability to scan the excitation fields (as shown at74), thecontrol computer54 may then direct the microscopy system to scan an area for a variety of different compositions, and the resulting pixel data is provided (as shown at74) to thecontrol computer54.

The modulation system may provide amplitude modulation of the first beam to provide a modulated pulse train such that only alternating pulses of the first pulse train are coincident with the pulses of the second pulse train. Such amplitude modulation of the first beam may be achieved using a Pockel cell and polarization analyzer as the modulator, and a Pockel cell driver as the controller.

If the modulation rate is of the same order of the repetition rate of the laser, countdown electronics must be utilized to guarantee the synchronization (phase) between the modulation and the pulse train. A wide variety of different modulation rates are also possible. In further embodiments, the contrast pulses may have an amplitude that is substantially zero by switching off the pulses at the modulation frequency, for example using an electro-optic modulator (such as a MEMs device or a galvanometric scanner) or an acousto-optic modulator.

Amplitude modulation of the pump or Stokes pulse trains may therefore be achieved, and the increase of the Stokes pulse train or decrease of the pump pulse train may be measured. By modulating the pump train of pulses and then detecting the Stokes train of pulses from the focal volume, Raman gain may be determined by the processing system. In an embodiment, the pump beam may be modulated, the Stokes beam may be detected from the focal volume, and Raman gain may be determined by the processing system. In a further embodiment, the Stokes beam may be modulated, the pump beam may be detected from the focal volume, and Raman loss may be determined by the processing system.

In spontaneous Raman scattering, the sample is excited with light at a single frequency ω_p. The output spectrum contains new radiation on both the Stokes (ω_S) and anti-Stokes sides (ω_nS) due to inelastic light scattering off molecular vibrations. In CRS, the combined action of pump and Stokes beams effectively transfers the molecules in the sample from the ground state into the targeted vibrational state. As a consequence a pump photon is absorbed and a Stokes photon is generated. This allows signal amplification by up 100,000× compared to spontaneous Raman scattering.

In contrast to fluorescence, the energy of the incident photons typically does not match an electronic excited state, and spontaneous Raman scattering is mediated through a virtual state rather than an excited electronic state, relying on vacuum fluctuations to generate the new emission. As such, spontaneous Raman scattering is extremely weak, resulting in long averaging times to obtain high signal to noise ratio (SNR) spectra and slow imaging speed in microscopy.

In CRS the sample is excited with two laser beams. The difference frequency, Δω=ω_P−ω_S, is tuned to match the frequency of a target vibration, Ω. In this case, the transition from the virtual state into the vibrational excited state is stimulated, not spontaneous, similar to the well-known phenomena of stimulated emission, which allows for light amplification in lasers. The molecular transition rate is consequently enhanced by r_coh/r_spo=n_Stokes+1, where n_Stokescorresponds to the number of photons in the optical mode of the Stokes beam and the +1 indicates spontaneous transitions. This is the origin for signal enhancement in CRS and the basis of fast label-free imaging. Ultimately n_Stokesand thus enhancement is limited by photodamage to ˜100,000× in biological specimen. This means that a complete high-resolution imaged may be acquired with CRS in the same time as a single spectrum with spontaneous Raman. Optimization of the laser parameters enables for high sensitivity and imaging speeds up to video-rate.

The two most popular CRS techniques, CARS and SRS, share this common excitation scheme and are, in fact, excited simultaneously. They differ in the detection. The CARS signal at the anti-Stokes frequency is detected by blocking the excitation beam with a high optical density (OD) filter after the sample. SRS, comprising stimulated Raman gain (SRG) of the Stokes beam and stimulated Raman loss (SRL) of the pump beam, are the intensity variations ΔI of the excitation beams I associated with the energy transfer from the optical field to the sample. Under biomedical excitation condition, the relative changes are however small (ΔI/I<10⁻⁴) and can be buried in the intensity fluctuations of the excitation lasers or the linear absorption or scattering of the sample.

A high-frequency modulation/detection scheme has therefore been developed to extract the SRS signal with high sensitivity as disclosed, for example, in U.S. Pat. No. 8,027,032, the disclosure of which is hereby incorporated by reference in its entirety. SRS is more sensitive than CARS, linear in the concentration of the target molecule and free from artifacts due to phase-matching. Detection is, however, somewhat more challenging. In particular, signal detection in reflection of thick, non-transparent samples (epi-detection) is more straight forward with CARS. As CARS and SRS share the same excitation conditions, the illumination systems of the present invention are applicable to both CRS techniques.

The input trains of laser pulses should be narowband (with a pulse that is longer than 0.5 ps). With reference toFIG. 2A both of the two synchronized trains oflaser pulses80,82, may be narrowband pulses, and as shown at86 and88, the increase in intensity of the Stokes illumination (ΔI_S) may be detected or the decrease in the pump illumination (ΔI_p) may be detected. As also shown inFIG. 2B, ω_p−ω_S=Ω. When both are narrowband, the difference frequency has to be adjusted, for example, by tuning either of the two center frequencies.

For CRS, theillumination system10 should provide the two trains of laser pulses ω_pand ω_S, one of which is modulated, and CRS occurs as long as Δω=ω_p−ω_Sfalls within the linewidth, typically ˜20 cm⁻¹(at 800 nm, 20 cm⁻¹=1.3 nm) of a Raman transition. As a consequence, a CRS laser source has to fulfill the following requirements. First, at least one of the laser beams has to be tunable to a precision narrower than about 0.2 nm. Second, the laser bandwidth has to be narrower than the typical Raman line-width (<3 nm). Third, the absolute wavelength is not critical, in contrast to spontaneous Raman scattering, which scales with 1/λ⁴. For biological samples however, it is advantages to excite the sample in the optical window from 700-1300 nm, where both scattering and absorption are minimal and resolution with a high numerical aperture (NA) lens is sub-cellular.

Achieving maximal sensitivity is a primary challenge for CRS microscopy. This is where most laser systems that fulfill the first and third criteria above fail. It is critical to consider the signal-to-noise-ratio (SNR) for various laser parameters. For continuous wave (CW) lasers, the SRS signal is proportional to the product of the average power of the pump and Stokes beams, i.e., SRS signal is nonlinear in the overall excitation power.

It is therefore advantageous to utilize pulsed lasers, which have high peak powers but moderate average powers to minimize heating effects in the sample due to linear absorption. For pulsed lasers with average power Ï_p,S, pulse duration duration τ and repetition rate R, SRS signal is proportional to the following:
(Î_p/τ·R)·(Î_S/τ·R)·τ·R=Î_p·Î_S/τ·R

High-frequency, phase-sensitive detection of SRS is close to shot-noise limited, i.e. noise for SRL is proportional to Ï_p^0.5and SNR∝Ï_S·Ï_p^0.5/τ·R. For a fixed total average power Î at the sample, it is thus advantageous to chose Î_Ssuch that

${\hat{I}}_S=2{\hat{I}}_p=\frac{2}{3}\hat{I}$
in order to maximize the SNR. A similar argument can be made for CARS microscopy, and it can be shown that for CRS microscopy, SNR∝Î^1.5/τ·R.

The use of a low repetition rate, femtosecond laser system would maximise the SNR, but a hard limit exists however, on the repetition rate for fast microscopy, that is set to R>10 MHz (and R>40 MHz for videorate imaging) by the fact that at least one laser pulse is required per pixel (and more if the laser repetition rate is not synchronized to of the pixel clock of the microscope). The pulse duration is limited by the time-bandwidth product, which states that a laser pulse of a given duration can only be achieved if it has a certain spectral bandwidth. Requirement (B) limits τ>0.5 ps. This requirement differentiates CRS from two-photon fluorescence, which is typically excited with femtosecond (fs) lasers.

Further, it is also important to consider the damage threshold of the sample. Optimizing the sensitivity for CRS means designing the CRS laser system to approach the damage threshold. While absolute quantification of photodamage is sample and metric dependent, studies suggest that near-IR laser damage in biological samples is primarily due to nonlinear absorption phenomena with a scaling of I_ave^γ/(τ·R)^γ-1and nonlinear scaling parameter γ being in the range from 2.5 to 3.5. The approximate measurements carried out with R=80 MHz, NA=1.2 and λ_pump=817 nm and Stokes λ_Stokes=1064 nm, indicate that the sample shows morphological changes after a single scan (the most drastic form of damage) for Î=25 mW at 180 fs, Î=80 mW at 1 ps, and Î=280 mW at bps. This suggests that γ≈3.2, i.e. the photodamage is more nonlinear than the CRS SNR. The maximal average power can thus be estimated by the experimental equation:

${\hat{I}}^{\max }\approx 25\mathrm{mW}·\left(\tau /180\mathrm{fs}·R/80\mathrm{MHz}\right)\frac{3.2-1}{3.2}\approx 25\mathrm{mW}·{\left(\tau /180\mathrm{fs}·R/80\mathrm{mHz}\right)}^{0.7}$

With the above assumptions, simulations were provided to determine the best pulse properties for CRS. It has been found that and found that within the hard limits (τ=1 ps-10 ps and R>20 MHz), the CRS SNR hardly varies with the exact parameter order to achieve the same signal with a 10-ps rather than 1-ps laser pulse however, the average power requirements of the laser system is increased ˜5×. Ideally the user can specify the system depending on whether it is to be optimized for high spectral resolution (i.e., larger τ and higher Î) or low average power, as for medical applications (i.e., shorter τ, lower R, and lower Î).

FIG. 3A shows at90 an estimated SNR as a function of pulse duration at a fixed repletion rate of 40 MHz and normalized to SNR for excitation with 80 HMz, 6 ps pulses. This assumes excitation with maximal average power that does not cause photo-damage, and neglects effects due to limited Raman line-width.FIG. 3B shows at92 an average power requirement for the laser system to achieve maximal SNR.

In summary, an ideal CRS laser system further requires a pulsed laser with pulse duration of 0.5-10 ps, repetition rate of 20-100 MHz, and sufficient average power given a certain pulse duration and repetition rate (FIG. 2B), as well as temporal synchronization of the pulse trains to a fraction of the pulse duration to avoid intensity fluctuations due to timing jitter.

In addition to the narrowband approach based on picosecond (ps) lasers, other modes of CRS microscopy are being investigated. A technique known as spectral focusing uses broadband frequency-chirped pulses, i.e., laser pulses with a center frequency that varies over time. If the chirp rate of both pump and Stokes pulses are matched, the frequency difference does not vary and is effectively narrowband, even though the absolute frequency is swept. Tuning the time delay changes the difference frequency, allowing different Raman peaks to be targeted. Spectral focusing is attractive for fast and reproducible spectral tuning or frequency modulation schemes.

Other approaches are based on exciting multiple vibrations simultaneously. In multiplex CRS, either the pump or Stokes beam is broadband, while the other is narrowband. By performing excitation or emission spectroscopy, signal from each vibration is detected separately. This approach allows for high chemical specificity and simultaneous multi-color imaging at a reduced imaging speed. There is a strong need for better laser sources for multiplex SRS, as most demonstrations are based on unstable electronic locking of fs- and ps-lasers.

The disclosed laser system can also be applied to other spectroscopies and label-free microscopy techniques, such as two-color, two-photon absorption (TPA) microscopy, stimulated emission (SE) microscopy, ground-state depletion (GD) microscopy and sum-frequency generation (SFG). These techniques have slightly different requirements on the laser system. Nevertheless, they share the common feature of two-color excitation with pulsed lasers.

TPA requires the sum-frequency of the two beams to be resonant with an electronic state of the sample (e.g., hemoglobin or melanin) and usually utilizes fs beams to further probe excited state dynamics. TPA is currently being explored for early detection of melanoma. SE/GD requires at least one of the two lasers to be tuned into an electronic absorption state of the sample.

All modulation transfer techniques (SRS, TPA, and SE/GD) further require a property (such as intensity, polarization or time delay) of one of the beams is modulated at a rate higher than 100 kHz allowing measurement the modulation transfer from this modulated beam to the second, originally un-modulated beam due to the nonlinear interaction in the sample.

The invention involves providing an illumination system for an imaging system such as a CRS imaging system that is economical and efficient to manufacture, and provides in particular, a CRS laser system design that is based on optical synchronization of two laser amplifiers. The approach starts with a laser oscillator with the first center frequency ω1.

This first laser is either a high-power laser (e.g., solid state Ti:Sa laser) or a low-power laser (e.g., fiber oscillator), which can be amplified to high power in consecutive steps (e.g., see reference for specific implementation). The output is split with an optical splitter. Part of the output provides the first pulse train for Coherent Raman Scattering Microscopy (i.e., either the pump or Stokes beam) or Modulation Transfer Microscopy (i.e., either the pump or probe beam). The other part of the output is fed into a frequency shifting unit, which generates optically synchronized light at a second optical frequency ω₂. The frequency shifting may for example, be achieved by super-continuum generation in a high nonlinear fiber (HNLF). This new light at ω₂is then used to seed a laser amplifier at this frequency.

FIG. 4, for example, shows an illumination system that includes alaser system14 and acombiner system20. Thelaser system14 includes alaser source100 that provides a first train of laser pulses. The first train of laser pulses is divided, and a portion is provided to afrequency shifting system102 and then to apicosecond amplifier system104. Amodulator106 may be employed to modulate the train of pulses responsive to a modulation signal36 (as discussed above), and in certain embodiments, the other signal may be modulated by amodulator108. Thecombiner system20 may include adelay unit110 that provides an adjustable delay to ensure that the first (e.g., Stokes) and second (e.g., pump) trains of laser pulses are temporally coincident with one another. Thetuning control signal70 is coupled to thepicosecond amplifier system104 for providing tuning control of the difference frequency between the excitation trains of laser pulses.

The laser source system may be a high power oscillator, or in other embodiments as shown inFIG. 5, thelaser source system120 may include alow power oscillator122 and anamplifier124 for providing the first train of laser pulses.

Thefrequency shifting system102 may include a highly non-linear fiber, or with reference toFIG. 6 may include afrequency shifting system134 that includes anamplifier136 and a highly non-linear fiber138 (e.g., a photonic crystal fiber). Thelaser system132 of thesystem130 provides a first train oflaser pulses144 as discussed above that is combined with a second train oflaser pulses142 from thepicosecond amplifier system140.

The picosecond amplifier system may include a narrowband amplifier system.FIG. 7, for example shows asystem150 that includes alaser system158 for providing a first train ofpulses164, afrequency shifting system160, and anarrowband amplifier system152 that includes anarrowband transmission filter154 and anamplifier156 for providing a second train oflaser pulses162. In other embodiments, the positions of theamplifier156 andnarrowband transmission filter154 may be reversed.

In accordance with a further embodiment and with reference toFIG. 8, asystem190 may include alaser system192 for providing a first train ofpulses194, afrequency shifting system196, and anarrowband amplifier system198 that includes acirculator200, anamplifier202 and anarrowband transmission filter204. The second train oflaser pulses206 is provided from thecirculator200 as shown.

In accordance with a yet a further embodiment and with reference toFIG. 9, asystem210 may include alaser system212 for providing a first train ofpulses214, afrequency shifting system216, and anarrowband amplifier system218 that includes afirst amplifier220, anarrowband filter222, and asecond amplifier224 that provides the second train ofpulses226. In accordance with a further embodiment and with reference toFIG. 10, asystem230 may include alaser system232 for providing a first train ofpulses234, afrequency shifting system236, and anarrowband amplifier system238 that includes afirst amplifier240, acirculator242, anarrowband filter244, asecond amplifier246 and a minor248. The second train oflaser pulses250 is provided from thecirculator242 as shown.

In accordance with a further embodiment and with reference toFIG. 11, asystem260 may include alaser system262 for providing a first train ofpulses264, afrequency shifting system266, and anarrowband amplifier system268 that includes acirculator270,first amplifier272, a narrowbandreflective filter274, and asecond amplifier276. The second train oflaser pulses278 is provided from thecirculator270 via thesecond amplifier276 as shown.

Additional embodiments for the illumination system include the following. As shown inFIG. 12, thesystem280 may include alaser system282 that provides a first train ofpulses286 via anamplifier284, and afrequency shifting system288 andnarrowband amplifier system290 for providing the second train oflaser pulses292. As shown inFIG. 13, thesystem300 may include alaser system302 that provides a first train of pulses308 via anarrowband filter304 and anamplifier306, and afrequency shifting system310 andnarrowband amplifier system312 for providing the second train of laser pulses314. As shown inFIG. 14, thesystem322 may include alaser system324 that provides a first train ofpulses330 via afirst amplifier324, anarrowband filter326 and asecond amplifier328, as well as afrequency shifting system330 and anarrowband amplifier system332 for providing the second train oflaser pulses334.

Additional embodiments of the invention include the following systems that employ frequency multiplication units.FIG. 15, for example shows asystem340 that includes alaser system342 that provides a first train ofpulses344 via a frequency doubling or triplingsystem346, as well as afrequency shifting system348 and anarrowband amplifier system350 for providing a second train oflaser pulses352.FIG. 16 shows asystem360 that includes alaser system362 that provides a first train ofpulses364, as well as afrequency shifting system366, anarrowband amplifier system368, and a frequency doubling or triplingsystem370 for providing a second train oflaser pulses372.

In accordance with various embodiments, the gain media for systems of the invention may include a variety of doped material. For example, and as shown in FIG.17, thesystem380 may include an Erbium-dopedlaser system382 for providing a first train oflaser pulses384 at 1530 nm-1610 nm (e.g., Stokes beam), as well as afrequency shifting system388 and aYtterbium amplifier system390 for providing a second train oflaser pulses392 at 1010 nm-1080 nm (e.g., pump beam). In accordance with an embodiment, thesystem380 may optionally further include an Erbium-dopedamplifier system386 for amplifying the first train of laser pulses as shown. The pump frequencies for the erbium-doped gain material may be about 980 nm, about 1480 nm or about 1550 nm (in-band pumping).

In further embodiments, the invention may provide asystem400 as shown inFIG. 18 that includes an Erbium-doped laser system (1530 nm-1610 nm), and an Erbium-doped amplifier system (1010 nm-1080 nm) and afrequency doubling system408 for providing the first train of laser pulses (e.g., pump beam). Thesystem400 also includes afrequency shifting system410 and aYtterbium amplifier system412 for providing the second train oflaser pulses414 at 1010 nm-1080 nm (e.g., Stokes beam).

In accordance with further embodiments, the invention may provide asystem420 shown inFIG. 19 including a Ytterbium-doped laser system422 (1010 nm-1080 nm), and a Ytterbium-doped amplifier system424 (1010 nm-1080 nm) for providing a first train of laser pulses (e.g., a Stokes beam). Thesystem420 also includes afrequency shifting system428, an Erbium-doped amplifier system430 (1530 nm-1610 nm) and afrequency doubling unit432 for providing a second train of laser pulses434 (e.g., a pump beam).

With reference again toFIG. 17, in accordance with further embodiments, thelaser system382 may instead be a Ytterbium-doped laser system (1010 nm-1080 nm) and theamplifier386 may be a Ytterbium-doped amplifier system (1010 nm-1080 nm) for providing, e.g., a Stokes beam. Theamplifier system390 may be a semiconductor amplifier system (700 nm-900 nm) that follows the frequency shifting system to provide the second train of pulses (e.g., pump beam.

In yet further embodiments, and again with reference toFIG. 17, thelaser system382 may be a titanium:sapphire (Ti:Sa) laser system (750 nm-950 nm) for directly providing a first train of laser pulses (e.g., pump beam), and theamplifier390 may be a Ytterbium-doped amplifier system (1010 nm-1080 nm) following thefrequency shifting system388 for providing the second train of laser pulses (e.g., Stokes beam). In further embodiments, thelaser system382 may be a Thulium fiber amplifier (1800 nm-2100 nm) or a Holmium fiber amplifier (1800 nm-2100 nm), each of which may be shiftable to 900 nm-1050 nm.

In an implementation of an all fiber illumination system of the invention in which the output from an Erbium-doped fiber-oscillator is split into two arms to seed Erbium-doped and Ytterbium-doped power amplifiers. Optical synchronization is provided by frequency shifting using supercontinuum (SC) generation in a highly nonlinear fiber (HNLF). Tunable narrowband output is achieved with an in-line filter. Detailed design criteria are discussed in the research plan.

In particular, theillumination system440 ofFIG. 20 includes alaser source system442, anamplifier444, and afrequency doubling unit446 for providing a first train of laser pulses448 (e.g., a pump beam). Thesystem440 also includesfrequency shifting system450 and anamplifier system452 for providing a second train of pulses454 (e.g., a Stokes beam). Thelaser source system442 may be an oscillator that includes awavelength division multiplexer460 that receives pump illumination (980 nm) as shown at462, anisolator464, a carbon nanotube saturable absorber466, anoutput coupler468, anotherisolator470, a 50/50fiber splitter472, and amodulator474.

The first train of laser pulses from the oscillator is provided to anErbium power amplifier444 that includes wavelength division multiplexer that receives pump illumination (e.g., 1480 nm) as shown at482, an Erbium-doped fiber (having normal dispersion)484 and acompression fiber486. The output of theErbium power amplifier444 is provided to a doublingcrystal446, which provides the first output train of laser pulses (pump beam) at 790 nm.

The second train of laser pulses from the oscillator is provided to thefrequency shifting unit450 that includes awavelength division multiplexer490 that receives pump illumination (e.g., 976 nm) as shown at492, an Erbium-doped fiber (normal dispersion), acompression filter496, a highlynon-linear fiber498 and a Ytterbium-dopedfiber500. The output of thefrequency shifting unit450 is provided to aYtterbium power amplifier452 that includes a tunablenarrowband filter502, awavelength division multiplexer504 that receives pump illumination (e.g., 980 nm) as shown at506, and a Ytterbium-dopedfiber508, which provides the second output train of laser pulses (Stokes beam) at 1010 nm-1080 nm. The first and second trains oflaser pulses448,454 are combined as discussed above.

The Erbium fiber oscillator with a center frequency of about 1550 nm and frequency bandwidth of about 10 nm is therefore employed, and this frequency is shifted to about 1040 nm to seed the Ytterbium fiber amplifier. Amplification of the Erbium signal may be performed before or after the optical splitter. Further, the Erbium signal may also be frequency doubled or tripled to achieve shorter wavelengths as preferred for microscopy. Center frequencies can further be shifted using passive or tunable optical filters or by gain engineering (e.g., in-band pumping).

Typically, CRI systems are based on narrowband, picosecond lasers. This means, that the laser frequency bandwidth is narrower than the typical bandwidth of Raman transitions (e.g., 1 nm). In the above laser system this may either be achieved by using a laser oscillator with narrow frequency bandwidth or by using a broad-band laser system in combination with narrowband optical filters, which can be fixed frequency or tunable. The reduced intensity by frequency filtering can be restored by additional laser amplifiers.

An alternative to narrowband CRS is a technique known as spectral focusing. Instead of using narrowband pulsed, one utilizes frequency chirped broadband pulses, i.e., laser pulses with varying center frequency over time. If the chirp rate of both pulses is matched, the frequency difference is fixed and narrowband, even though the absolute frequency is swept. By tuning the time delay, different difference frequencies, i.e., different Raman peaks may be targeted. As such spectral focusing may be used to achieve fast and reproducible spectral acquisition as desired for spectroscopic differentiation with CRS. Instead of a narrowband system. Systems of the invention may also provide pulses for spectral focusing CRS by using broadband amplifiers and adjusting the chirp rate with a dispersion unit (e.g., optical fibers with well-known dispersion). The delay may be scanned automatically or manually with an optical delay stage.

Laser systems of various embodiments of the invention may also provide excitation pulses for multiplex CRS. In multiplex CRS, either pump or Stokes beam is broadband and narrowband, respectively. This means that multiple Raman transitions are excited simultaneously. By performing either excitation or emission spectroscopy, it is possible to detect the signal from each vibrations separately and simultaneously, i.e., in a multiplexed fashion. This means that multicolor images at multiple vibrations may be acquired. In another implementation of the disclosed laser system multiplex CRS can be achieved by implementing a broadband first train of pulses and a narrowband second train of pulses or vice versa.

As discussed above with reference toFIG. 4, either of the first and second trains of laser pulses may pass through an optical delay device to ensure coincident timing when the trains of laser pulses are combined, and either the first train of laser pulses or the second train of laser pulses may be modulated prior to combining the two trains of laser pulses.

FIG. 21A shows at510 the narrow bandwidth of the output spectra and the tuning range for the Erbium-doped amplifier, andFIG. 21B shows at512 the narrow bandwidth and tuning range for the Ytterbium-doped amplifier.

In accordance with further embodiments, the picosecond amplifier may include a chirped amplifier system, and the illumination system may be used in a spectral focusing imaging system, which requires lasers with a bandwidth smaller or comparable to the typical Raman linewidth. If broadband lasers are chirped linearly however, (i.e., the instantaneous laser frequency changes over time), and that the same rate for both pump and Stokes, the difference frequency is essentially narrowband. Rather than tuning the difference by changing the center frequency of the lasers, CRI spectra may be acquired by tuning the time delay.

FIG. 22 shows that the frequency of each of the pump and Stokes fields may be varied over time and frequency, yet provide the same difference frequency as shown at514 and516 inFIG. 22. This provides for spectral focusing. Specifically, it shows that an amplified frequency-shifted train of pulses may be provided having pulses with a temporally evolving instantaneous frequency over the picosecond pulse duration.

FIG. 23, for example, shows a system in accordance with a further embodiment of the present invention that includes a laser system (as discussed above) that provides a first train of laser pulses to achirped control system524, which provide the first output train oflaser pulses526. Thesystem520 also includes a frequency shifting system528 (as discussed above) and achirped amplifier system530 that provides the second output train oflaser pulses532. Thelaser system522, the gain media, and thefrequency shifting system528, may be as described above with reference toFIGS. 1-20.

As shown inFIG. 24, asystem540 in accordance with a further embodiment may include alaser system542 and achirped control system544 for providing the first train oflaser pulses546. Thesystem540 may also include afrequency shifting system548, as well as achirped amplifier system550 that includes a chirp unit552 (e.g., a fiber or prism pain) and anamplifier554, which provides the second output train oflaser pulses556. Again, thelaser system542, the gain media, and thefrequency shifting system548, may be as described above with reference toFIGS. 1-20.

FIG. 25 shows asystem560 in accordance with a further embodiment that includes alaser system562 and achirped control system564 for providing the first train oflaser pulses566. Thesystem560 also includes afrequency shifting system568, as well as achirped amplifier system570 that includes abroadband amplifier572, chirp unit572 (e.g., a fiber or prism pain) and anamplifier574, which provides the second output train oflaser pulses576. Again, thelaser system562, the gain media, and thefrequency shifting system568, may be as described above with reference toFIGS. 1-20.

FIG. 26 shows asystem580 in accordance with a further embodiment that includes alaser system582 and achirped control system584 for providing the first train oflaser pulses586. Thesystem580 also includes afrequency shifting system588, as well as achirped amplifier system590 that includes abroadband amplifier592, anamplifier594, frequency doubling or triplingunit596, and a chirp unit598 (e.g., a fiber or prism pain), which provides the second output train oflaser pulses600. Again, thelaser system582, the gain media, and thefrequency shifting system588, may be as described above with reference toFIGS. 1-20, and the frequency doubling or triplingunit596 may be as discussed above with reference toFIGS. 15 and 16.

FIG. 27 shows asystem610 in accordance with a further embodiment that includes alaser system612 and achirped control system614 that includes a chirp unit616 (e.g., fiber or prism pain) for providing the first train oflaser pulses618. Thesystem610 also includes afrequency shifting system620, as well as achirped amplifier system622, which provides the second output train oflaser pulses624. Again, the laser system, the gain media,612 and thefrequency shifting system620, may be as described above with reference toFIGS. 1-20.

FIG. 28 shows asystem630 in accordance with a further embodiment that includes alaser system632 and achirped control system634 that includes a chirp unit616 (e.g., fiber or prism pain) and anamplifier638 for providing the first train oflaser pulses640. Thesystem630 also includes afrequency shifting system642, as well as achirped amplifier system644, which provides the second output train oflaser pulses646. Again, thelaser system632, the gain media, and thefrequency shifting system642, may be as described above with reference toFIGS. 1-20.

FIG. 29 shows asystem650 in accordance with a further embodiment that includes alaser system652 and achirped control system654 that includes abroadband filter656, a chirp unit658 (e.g., fiber or prism pain) and anamplifier660 for providing the first train oflaser pulses662. Thesystem650 also includes a frequency shifting system664, as well as achirped amplifier system666, which provides the second output train oflaser pulses668. Again, thelaser system652 and the frequency shifting system664, the gain media, may be as described above with reference toFIGS. 1-20.

FIG. 30 shows asystem670 in accordance with a further embodiment that includes alaser system672 and achirped control system674 that includes abroadband filter676, anamplifier678, a frequency doubling or triplingunit680, and a chirp unit682 (e.g., fiber or prism pain) for providing the first train oflaser pulses684. Thesystem670 also includes afrequency shifting system686, as well as achirped amplifier system688, which provides the second output train oflaser pulses690. Again, thelaser system672, the gain media, and thefrequency shifting system686, may be as described above with reference toFIGS. 1-20.

FIG. 31 shows at700 an image taken using a CRS system with an illumination system in accordance with an embodiment of the present invention wherein the size is 1 μm, the sampling was 512 by 512 pixels, and the imaging speed was one frame per second (4 μs/pixel).FIG. 32 shows at710 a cross-section of theimage700 with a signal to noise ratio of greater than 25.

FIG. 33A shows at720 an image taken using a CRS system with an illumination system in accordance with an embodiment of the present invention of CH2 vibrations (lipids) using a Stokes beam of 1018 nm, a pump beam of 789 nm and a wavenumber of 2850 CM⁻¹.FIG. 33B shows at722 an image taken using a CRS system with an illumination system in accordance with an embodiment of the present invention of CH3 vibrations (proteins) using a Stokes beam of 1028 nm, a pump beam of 789 nm and a wavenumber of 2950 CM⁻¹.FIG. 34 shows at730 a combination of theimages720,722 ofFIGS. 33A and 33B.

Those skilled in the art will appreciate that numerous modifications and variations may be made to the above disclosed embodiments without departing from the spirit and scope of the claims.

Claims (21)

What is claimed is:

1. An illumination system for providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy, said illumination system comprising:

a laser system including a laser oscillator for providing a first train of pulses at a center optical frequency ω₁;

an optical splitting means for dividing the first train of pulses at the center optical frequency ω₁into a first split train of pulses and a second split train of pulses;

a frequency shifting system for shifting the optical frequency of the first split train of pulses to provide a frequency shifted train of pulses; and

a picosecond amplifier system for amplifying the frequency shifted train of pulses to provide an amplified frequency-shifted train of pulses having a pulse duration of at least 0.5 picoseconds.

2. The illumination system as claimed inclaim 1, wherein said picosecond amplifier is a narrowband amplifier.

3. The illumination system as claimed inclaim 2, wherein said narrowband amplifier is tunable to provide that the difference frequency of the amplified frequency-shifted train of pulses and the second split train of pulses may be varied.

4. The illumination system as claimed inclaim 2, wherein said narrowband amplifier system includes a narrowband filter.

5. The illumination system as claimed inclaim 2, wherein said narrowband amplifier system includes a plurality of amplifiers in series.

6. The illumination system as claimed inclaim 1, wherein said laser system includes a high power oscillator.

7. The illumination system as claimed inclaim 1, wherein said laser system includes a low power oscillator and an amplifier.

8. The illumination system as claimed inclaim 1, wherein said illumination system further includes a laser amplifier for amplifying the second split train of pulses.

9. The illumination system as claimed inclaim 1, wherein said frequency shifting system includes a highly non-linear fiber.

10. The illumination system as claimed inclaim 1, wherein said frequency shifting system includes a photonic crystal fiber.

11. The illumination system as claimed inclaim 1, wherein said frequency shifting system further includes a laser amplifier.

12. The illumination system as claimed inclaim 1, wherein said picosecond amplifier is a chirp amplifier to provide an amplified frequency-shifted train of pulses having pulses with a temporally evolving instantaneous frequency over the picosecond pulse duration.

13. The illumination system as claimed inclaim 12, wherein said chirped amplifier system includes a chirp unit and an amplifier.

14. The illumination system as claimed inclaim 13, wherein said chirped amplifier system further includes a broadband filter.

15. The illumination system as claimed inclaim 14, wherein system further includes a chirp control system in the second split train of pulses.

16. The illumination system as claimed inclaim 1, wherein system further includes:

a combiner for combining the amplified frequency-shifted train of pulses and the second split train of pulses from the optical splitting means; and

adjusting means for adjusting a time delay between the amplified frequency-shifted train of pulses and the second split train of pulses with sub-picosecond precision to provide a collinear output of the dual-excitation wavelength illumination for the non-linear optical microscopy or micro-spectroscopy system.

17. The illumination system as claimed inclaim 1, wherein said laser system is one of a Ytterbium fiber laser and an Erbium fiber laser.

18. The illumination system as claimed inclaim 1, wherein said laser system is a Titanium Sapphire laser.

19. The illumination system as claimed inclaim 1, wherein said picosecond amplifier system is one of an Erbium fiber amplifier, a Ytterbium fiber amplifier, a Thulium fiber amplifier, and a Holmium fiber amplifier.

20. The illumination system as claimed inclaim 1, wherein the output of the picosecond amplifier system is frequency doubled (SHG) or tripled (THG).

21. The illumination system as claimed inclaim 1, wherein the second split train of pulses from the optical splitting means is frequency doubled (SHG) or tripled (THG).

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