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US3874852A - Reagent and method for determining leukocytes and hemoglobin in the blood - Google Patents

Reagent and method for determining leukocytes and hemoglobin in the bloodDownload PDF

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Publication number
US3874852A
US3874852AUS485931AUS48593174AUS3874852AUS 3874852 AUS3874852 AUS 3874852AUS 485931 AUS485931 AUS 485931AUS 48593174 AUS48593174 AUS 48593174AUS 3874852 AUS3874852 AUS 3874852A
Authority
US
United States
Prior art keywords
ion
quaternary ammonium
reagent
chain alkyl
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US485931A
Inventor
Thomas E Hamill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Diagnostics Inc
Original Assignee
Coulter Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filedlitigationCriticalhttps://patents.darts-ip.com/?family=23929974&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=US3874852(A)"Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Coulter Diagnostics IncfiledCriticalCoulter Diagnostics Inc
Priority to US485931ApriorityCriticalpatent/US3874852A/en
Priority to NL7415233.Aprioritypatent/NL164669B/en
Priority to AU75725/74Aprioritypatent/AU482810B2/en
Priority to ZA00747574Aprioritypatent/ZA747574B/en
Priority to IL46137Aprioritypatent/IL46137A/en
Priority to IT54278/74Aprioritypatent/IT1029631B/en
Priority to CH1585074Aprioritypatent/CH588078A5/xx
Priority to JP754195Aprioritypatent/JPS616341B2/ja
Priority to CA217,470Aprioritypatent/CA1024868A/en
Priority to FR7500452Aprioritypatent/FR2277344A1/en
Priority to BE6044886Aprioritypatent/BE824206A/en
Priority to SE7500245Aprioritypatent/SE415609B/en
Priority to MX754018Uprioritypatent/MX4259E/en
Priority to ES433929Aprioritypatent/ES433929A1/en
Priority to GB2184/75Aprioritypatent/GB1489303A/en
Priority to DE2501855Aprioritypatent/DE2501855C3/en
Publication of US3874852ApublicationCriticalpatent/US3874852A/en
Application grantedgrantedCritical
Publication of US3874852B1publicationCriticalpatent/US3874852B1/en
Anticipated expirationlegal-statusCritical
Expired - Lifetimelegal-statusCriticalCurrent

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Abstract

Leukocytes and hemoglobin in the blood are determined in vitro with a reagent which comprises a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion in amounts sufficient to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for the determinations.

Claims (8)

US485931A1974-07-051974-07-05Reagent and method for determining leukocytes and hemoglobin in the bloodExpired - LifetimeUS3874852A (en)

Priority Applications (16)

Application NumberPriority DateFiling DateTitle
US485931AUS3874852A (en)1974-07-051974-07-05Reagent and method for determining leukocytes and hemoglobin in the blood
NL7415233.ANL164669B (en)1974-07-051974-11-22 METHOD FOR PREPARING A REAGENT SUITABLE FOR DETERMINING LEUKOCYTES AND HEMOGLOBINE IN BLOOD.
AU75725/74AAU482810B2 (en)1974-07-051974-11-25Reagent and method for determining leukocytes and hemoglobin inthe blood
ZA00747574AZA747574B (en)1974-07-051974-11-27Reagent and method for determining leukocytes and hemoglobin in the blood
IL46137AIL46137A (en)1974-07-051974-11-27Reagent and method for determining leukocytes and hemoglobin in the blood
IT54278/74AIT1029631B (en)1974-07-051974-11-28 REGENT TO DETERMINE LEUCOCYTES AND HEMOGLOBIN IN THE BLOOD
CH1585074ACH588078A5 (en)1974-07-051974-11-29
JP754195AJPS616341B2 (en)1974-07-051974-12-28
CA217,470ACA1024868A (en)1974-07-051975-01-07Reagent and method for determining leukocytes and hemoglobin in the blood
FR7500452AFR2277344A1 (en)1974-07-051975-01-08 REAGENT AND METHOD FOR DETERMINING LEUCOCYTES AND HEMOGLOBIN IN BLOOD
BE6044886ABE824206A (en)1974-07-051975-01-08 METHOD FOR PREPARING A REAGENT AND METHOD FOR DETERMINING LEUKOCYTE AND HEMOGLOBIN IN BLOOD
SE7500245ASE415609B (en)1974-07-051975-01-10 SET AND REAGENTS FOR DETERMINATION OF LEUKOCYTES AND HEMOGLOBIN IN BLOOD
MX754018UMX4259E (en)1974-07-051975-01-15 IMPROVED PROCEDURE FOR OBTAINING A COMPOSITION FOR Erythrocyte and Thrombocyte Stromatolysis in Blood
ES433929AES433929A1 (en)1974-07-051975-01-17Reagent and method for determining leukocytes and hemoglobin in the blood
GB2184/75AGB1489303A (en)1974-07-051975-01-17Reagent and method for determining leukocytes and hemoglobin in the blood
DE2501855ADE2501855C3 (en)1974-07-051975-01-17 Reagent and method for the determination of leukocytes and hemoglobin in blood

Applications Claiming Priority (1)

Application NumberPriority DateFiling DateTitle
US485931AUS3874852A (en)1974-07-051974-07-05Reagent and method for determining leukocytes and hemoglobin in the blood

Publications (2)

Publication NumberPublication Date
US3874852Atrue US3874852A (en)1975-04-01
US3874852B1 US3874852B1 (en)1989-07-11

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ID=23929974

Family Applications (1)

Application NumberTitlePriority DateFiling Date
US485931AExpired - LifetimeUS3874852A (en)1974-07-051974-07-05Reagent and method for determining leukocytes and hemoglobin in the blood

Country Status (15)

CountryLink
US (1)US3874852A (en)
JP (1)JPS616341B2 (en)
BE (1)BE824206A (en)
CA (1)CA1024868A (en)
CH (1)CH588078A5 (en)
DE (1)DE2501855C3 (en)
ES (1)ES433929A1 (en)
FR (1)FR2277344A1 (en)
GB (1)GB1489303A (en)
IL (1)IL46137A (en)
IT (1)IT1029631B (en)
MX (1)MX4259E (en)
NL (1)NL164669B (en)
SE (1)SE415609B (en)
ZA (1)ZA747574B (en)

Cited By (39)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US4102810A (en)*1975-01-131978-07-25Coulter Electronics, Inc.Stabilized hematological reagent solutions
US4141856A (en)*1976-05-241979-02-27Dorwart Jr William VReference material for establishing anion concentrations in analytical chemistry tests
US4185964A (en)*1977-02-081980-01-29Central Laboratories of Associated Maryland Pathologists, Ltd.Lysing reagent
US4219440A (en)*1979-06-061980-08-26Coulter Electronics, Inc.Multiple analysis hematology reference control reagent and method of making the same
EP0014929A1 (en)*1979-02-141980-09-03Roche Diagnostics GmbHDiagnostic means, process for its preparation and its use in determining leucocytes in body fluids
US4286963A (en)*1979-11-231981-09-01Coulter Electronics, Inc.Differential lymphoid-myeloid determination of leukocytes in whole blood
DE3123669A1 (en)*1980-06-161982-04-22Coulter Electronics, Inc., 33010 Hialeah, Fla. METHOD, MULTI-PURPOSE BLOOD DILUTION AND METHOD, REAGENT FOR THE DIFFERENTIAL DETERMINATION OF THE LYMPHOID-MYELOID POPULATION OF LEUKOCYTES
US4455376A (en)*1979-09-171984-06-19R. J. Harvey Instrument Corp.Photometric methods for counting the particulate components of blood
WO1984002777A1 (en)*1983-01-031984-07-19Coulter ElectronicsMethod for the volumetric differential of leukocytes
WO1984003771A1 (en)*1983-03-251984-09-27Coulter ElectronicsMethod and stromatolysing reagent for the volumetric determination of populations of leukocytes
WO1984004969A1 (en)*1983-06-061984-12-20Coulter ElectronicsReagent for combined diluting and lysing whole blood
US4521518A (en)*1980-06-161985-06-04Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4526869A (en)*1980-09-241985-07-02Regents Of The University Of MinnesotaMethod for quantitatively determining the concentration of hemoglobin in a biological sample
US4543336A (en)*1982-02-121985-09-24Dragerwerk AktiengesellschaftIndicator for determining sulfur dioxide
WO1985005640A1 (en)*1984-05-311985-12-19Coulter Electronics, Inc.A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes
US4617275A (en)*1983-09-291986-10-14Toa Medical Electronics Co., Ltd.Reagent for blood analysis
FR2582103A1 (en)*1985-03-191986-11-21Canavaggio Michel METHOD FOR DETERMINING A BIOLOGICAL SUBSTANCE IN A SAMPLE
US4637986A (en)*1983-08-221987-01-20Ortho Diagnostic Systems, Inc.Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
US4725555A (en)*1986-10-221988-02-16Helena Laboratories CorporationZinc protoporphyrin test system
US4745071A (en)*1985-09-051988-05-17Sequoia-Turner CorporationMethod for the volumetric differentiation of blood cells types
WO1988007187A1 (en)*1987-03-131988-09-22Coulter Electronics, Inc.System for isolating and identifying leukocytes
US4853338A (en)*1987-05-201989-08-01Technicon Instruments CorporationCyanide-free hemoglobin reagent
US4962038A (en)*1980-06-161990-10-09Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US5008202A (en)*1988-11-291991-04-16Sequoia Turner CorporationBlood diluent for red blood cell analysis
US5045474A (en)*1987-05-281991-09-03Sequoia-Turner Corporation (Unipath)Semi-automatic process for white cell differential count
US5155044A (en)*1987-03-131992-10-13Coulter Electronics, Inc.Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US5284940A (en)*1990-11-141994-02-08Hri Research, Inc.Preparation for nucleic acid samples
US5510267A (en)*1989-05-151996-04-23Abbott LaboratoriesFlow cytometry lytic agent and method enabling 5-part leukocyte differential count
US5620852A (en)*1990-11-141997-04-15Hri Research, Inc.Nucleic acid preparation methods
US5654179A (en)*1990-11-141997-08-05Hri Research, Inc.Nucleic acid preparation methods
US5834315A (en)*1994-12-231998-11-10Coulter CorporationCyanide-free reagent and method for hemoglobin determination and leukocyte differentitation
EP0878715A1 (en)*1997-05-131998-11-18Bayer CorporationCyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers
US5958781A (en)*1994-07-141999-09-28Abbott LaboratoriesMethods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood
US20040241769A1 (en)*1999-09-242004-12-02Crews Harold RichardsonMulti-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
US20050106713A1 (en)*2003-09-032005-05-19Phan Brigitte C.Personal diagnostic devices and related methods
WO2014006638A1 (en)2012-07-032014-01-09Srivastava AmbarDevice and method for blood hemoglobin measurement without carboxyhemoglobin interference
US9091677B2 (en)2010-08-092015-07-28Beckman Coulter, Inc.Isotonic buffered composition and method that enables counting of cells
US9523676B2 (en)2011-08-242016-12-20Eiken Kagaku Kabushiki KaishaLeukocyte measurement device and reagent kit
CN113959911A (en)*2020-07-202022-01-21深圳迈瑞生物医疗电子股份有限公司Detection method and reagent for resisting platelet aggregation interference and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
DE3024835A1 (en)*1980-07-011982-01-28Bayer Ag, 5090 Leverkusen REAGENT FOR HEMOGLOBIN DETERMINATION

Citations (3)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US3546131A (en)*1968-07-051970-12-08Uni Tech Chem Mfg CoStabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone
US3607695A (en)*1969-02-141971-09-21Pfizer & Co CHemoglobinopathy controls
US3663175A (en)*1970-10-051972-05-16Bio Dynamics IncMethod of determining hemoglobin in blood

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
BE790042A (en)*1971-10-141973-04-13Coulter Electronics METHOD FOR CLASSIFYING PARTICLES

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US3546131A (en)*1968-07-051970-12-08Uni Tech Chem Mfg CoStabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone
US3607695A (en)*1969-02-141971-09-21Pfizer & Co CHemoglobinopathy controls
US3663175A (en)*1970-10-051972-05-16Bio Dynamics IncMethod of determining hemoglobin in blood

Cited By (56)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US4102810A (en)*1975-01-131978-07-25Coulter Electronics, Inc.Stabilized hematological reagent solutions
US4141856A (en)*1976-05-241979-02-27Dorwart Jr William VReference material for establishing anion concentrations in analytical chemistry tests
US4185964A (en)*1977-02-081980-01-29Central Laboratories of Associated Maryland Pathologists, Ltd.Lysing reagent
EP0014929A1 (en)*1979-02-141980-09-03Roche Diagnostics GmbHDiagnostic means, process for its preparation and its use in determining leucocytes in body fluids
US4219440A (en)*1979-06-061980-08-26Coulter Electronics, Inc.Multiple analysis hematology reference control reagent and method of making the same
US4455376A (en)*1979-09-171984-06-19R. J. Harvey Instrument Corp.Photometric methods for counting the particulate components of blood
US4286963A (en)*1979-11-231981-09-01Coulter Electronics, Inc.Differential lymphoid-myeloid determination of leukocytes in whole blood
US4521518A (en)*1980-06-161985-06-04Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4962038A (en)*1980-06-161990-10-09Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
DE3153618C2 (en)*1980-06-161992-10-15Coulter Electronics, Inc., Hialeah, Fla., Us
DE3123669A1 (en)*1980-06-161982-04-22Coulter Electronics, Inc., 33010 Hialeah, Fla. METHOD, MULTI-PURPOSE BLOOD DILUTION AND METHOD, REAGENT FOR THE DIFFERENTIAL DETERMINATION OF THE LYMPHOID-MYELOID POPULATION OF LEUKOCYTES
US4346018A (en)*1980-06-161982-08-24Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
GB2147999A (en)*1980-06-161985-05-22Coulter ElectronicsLysing reagent for differential determination of lymphoid-myeloid population of leukocytes
US4526869A (en)*1980-09-241985-07-02Regents Of The University Of MinnesotaMethod for quantitatively determining the concentration of hemoglobin in a biological sample
US4543336A (en)*1982-02-121985-09-24Dragerwerk AktiengesellschaftIndicator for determining sulfur dioxide
US4528274A (en)*1982-07-061985-07-09Coulter Electronics, Inc.Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4485175A (en)*1983-01-031984-11-27Coulter Electronics, Inc.Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
WO1984002777A1 (en)*1983-01-031984-07-19Coulter ElectronicsMethod for the volumetric differential of leukocytes
WO1984003771A1 (en)*1983-03-251984-09-27Coulter ElectronicsMethod and stromatolysing reagent for the volumetric determination of populations of leukocytes
WO1984004969A1 (en)*1983-06-061984-12-20Coulter ElectronicsReagent for combined diluting and lysing whole blood
US4529705A (en)*1983-06-061985-07-16Coulter Electronics, Inc.Reagent for combined diluting and lysing whole blood
EP0136058A3 (en)*1983-08-221987-04-22Ortho Diagnostic Systems Inc.Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part wbc count
US4637986A (en)*1983-08-221987-01-20Ortho Diagnostic Systems, Inc.Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
US4656139A (en)*1983-09-291987-04-07Toa Medical Electronics Co., Ltd.Method for preparing cells for blood analysis
US4617275A (en)*1983-09-291986-10-14Toa Medical Electronics Co., Ltd.Reagent for blood analysis
WO1985005640A1 (en)*1984-05-311985-12-19Coulter Electronics, Inc.A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes
FR2582103A1 (en)*1985-03-191986-11-21Canavaggio Michel METHOD FOR DETERMINING A BIOLOGICAL SUBSTANCE IN A SAMPLE
US4745071A (en)*1985-09-051988-05-17Sequoia-Turner CorporationMethod for the volumetric differentiation of blood cells types
US4725555A (en)*1986-10-221988-02-16Helena Laboratories CorporationZinc protoporphyrin test system
EP0268376A3 (en)*1986-10-221990-07-11Helena Laboratories CorporationBlood sample modification and reagent compositions therefor
WO1988007187A1 (en)*1987-03-131988-09-22Coulter Electronics, Inc.System for isolating and identifying leukocytes
US5155044A (en)*1987-03-131992-10-13Coulter Electronics, Inc.Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US5731206A (en)*1987-03-131998-03-24Coulter Electronics, Inc.Method and reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US4853338A (en)*1987-05-201989-08-01Technicon Instruments CorporationCyanide-free hemoglobin reagent
US5045474A (en)*1987-05-281991-09-03Sequoia-Turner Corporation (Unipath)Semi-automatic process for white cell differential count
US5008202A (en)*1988-11-291991-04-16Sequoia Turner CorporationBlood diluent for red blood cell analysis
US5510267A (en)*1989-05-151996-04-23Abbott LaboratoriesFlow cytometry lytic agent and method enabling 5-part leukocyte differential count
US5284940A (en)*1990-11-141994-02-08Hri Research, Inc.Preparation for nucleic acid samples
US5620852A (en)*1990-11-141997-04-15Hri Research, Inc.Nucleic acid preparation methods
US5654179A (en)*1990-11-141997-08-05Hri Research, Inc.Nucleic acid preparation methods
US5958781A (en)*1994-07-141999-09-28Abbott LaboratoriesMethods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood
US6740527B1 (en)1994-07-142004-05-25Abbott LaboratoriesMethods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood
US5834315A (en)*1994-12-231998-11-10Coulter CorporationCyanide-free reagent and method for hemoglobin determination and leukocyte differentitation
EP0878715A1 (en)*1997-05-131998-11-18Bayer CorporationCyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers
US5858794A (en)*1997-05-131999-01-12Bayer CorporationCyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers
US20040241769A1 (en)*1999-09-242004-12-02Crews Harold RichardsonMulti-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells
WO2005084534A1 (en)2003-09-032005-09-15Life Patch International, Inc.Personal diagnostic devices and related methods
US20050106713A1 (en)*2003-09-032005-05-19Phan Brigitte C.Personal diagnostic devices and related methods
US9133024B2 (en)2003-09-032015-09-15Brigitte Chau PhanPersonal diagnostic devices including related methods and systems
US9993189B2 (en)2003-09-032018-06-12Life Patch InternationalPersonal diagnostic device having a fluidic circuit with a plurality of analysis chambers
US11737694B2 (en)2003-09-032023-08-29Life Patch International, Inc.Personal diagnostic device having a plurality of tubules
US9091677B2 (en)2010-08-092015-07-28Beckman Coulter, Inc.Isotonic buffered composition and method that enables counting of cells
US9567622B2 (en)2010-08-092017-02-14Beckman Coulter, Inc.Isotonic buffered composition and method that enables counting of cells
US9523676B2 (en)2011-08-242016-12-20Eiken Kagaku Kabushiki KaishaLeukocyte measurement device and reagent kit
WO2014006638A1 (en)2012-07-032014-01-09Srivastava AmbarDevice and method for blood hemoglobin measurement without carboxyhemoglobin interference
CN113959911A (en)*2020-07-202022-01-21深圳迈瑞生物医疗电子股份有限公司Detection method and reagent for resisting platelet aggregation interference and application thereof

Also Published As

Publication numberPublication date
ES433929A1 (en)1976-11-16
SE415609B (en)1980-10-13
FR2277344B1 (en)1977-11-18
JPS616341B2 (en)1986-02-25
DE2501855A1 (en)1976-01-22
SE7500245L (en)1976-01-07
GB1489303A (en)1977-10-19
CH588078A5 (en)1977-05-31
MX4259E (en)1982-03-08
IL46137A0 (en)1975-02-10
CA1024868A (en)1978-01-24
IT1029631B (en)1979-03-20
IL46137A (en)1977-02-28
BE824206A (en)1975-07-08
DE2501855C3 (en)1980-10-16
DE2501855B2 (en)1980-02-07
US3874852B1 (en)1989-07-11
JPS516788A (en)1976-01-20
FR2277344A1 (en)1976-01-30
NL7415233A (en)1976-01-07
NL164669B (en)1980-08-15
AU7572574A (en)1976-05-27
ZA747574B (en)1976-07-28

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Legal Events

DateCodeTitleDescription
RRRequest for reexamination filed

Effective date:19840919

B1Reexamination certificate first reexamination
RFReissue application filed

Effective date:19891130
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