US3780935A - Serum separating method - Google Patents

Abstract

The separation of a sample of blood into serum and clot portions is accomplished by means of a sealant consisting essentially of a silicone fluid and silica dispersed therein. The separation is accomplished by inserting a device containing a supply of the sealant into a container holding a sample of the blood, the device being characterized by a nozzle portion which extends into the sample. The container and device are centrifuged so that it separates into serum and clot portions and the sealant, having a specific gravity of at least 1.026, separates the two portions.

Description

United States Patent [191 Lukacs et al.

[ Dec. 25, 1973 SERUM SEPARATING METHOD Inventors: Michel J. Lukacs, Goshen, N.Y.; [an

H. Jacoby, Franklin Lakes, NJ.

Assignee: Lukacs & Jacoby Associates,

Goshen, NY.

Filed: July 10, 1972 Appl. No.: 270,278

US. Cl 233/1 A, 210/65, 210/83 Int. Cl 801d 21/26 Field of Search 106/287 SB; 210/65,

210/83, DIG. 23; 233/1, 26

References Cited UNITED STATES PATENTS 3/1972 Adler ..2l0/83 4/1970 Coleman ..2lO/83 Primary Examiner-Samih N. Zahama Assistant Examiner-Robert G. Mukai Attorney.lohn A. Mitchell et al.

ABSTRACT 3 Claims, 8 Drawing Figures PATENTED M825 I975 FIG. 4

FIG. 2 FIG. 3

SERUM SEPARATING METHOD BACKGROUND OF THE INVENTION This invention relates to a method, apparatus and composition of matter for separating a blood sample by centrifuging into clot portions, so that the serum may be quickly and readily removed without contamination by the clot portion.

In recent years, biomedical and hospital laboratories have been faced with increasing demands for more and more routine, as well as specialized, diagnostic tests of blood samples. To meet the demands of these tests equipment has been devised which automatically takes a sample or specimen of blood which has been placed in a cup and subjects it to a series of programmed tests which eventuate in a readout on a record member. While these analyzers have increased the efficiency of performing the necessary tests, a problem has continued in finding ways and means of separating the serum from the clot portion and removing the serum for analysis. Various types of tube and plug devices have been suggested by the prior art. For example, in U.S. Pat. No. 3,512,940, issued May 19, 1970, a device consisting of a tube with a filter at one end thereof is inserted into a second but larger diameter tube containing a sample of the material desired to be filtered. In U.S. Pat. No. 3,508,653, issued Apr. 28, 1970, a piston in the form of a solid plug is driven through a centrifuged blood sample so as to position itself between the serum and clot portions of the centrifuged sample. The tube within the tube concept of US. Pat. No. 3,512,940 suffers from the apparent deficiency of being costly and not readily adaptable for disposal after a single use. The plug' arrangement of US. Pat. No. 3,508,653 has the shortcoming of utilizing a solid plug member which when subjected to a substantial centrifugal force, may also develop radial forces acting against the side of the sample tube, thus creating the danger of breakage.

SUMMARY OF THE INVENTION a silicone fluid and silica. The sealant has a specific gravity of at least 1.026 and preferably in the range of 1.030 to 1.050. As such, it will normally be at the proper specific gravity to divide the serum and clot portions of the centrifuged sample, sealing the clot in the container while the serum is removed.

Accordingly, it is an object of the present invention to provide a simple and effective method of obtaining a serum sample during centrifuging.

DESCRIPTION OF THE DRAWINGS FIG. 4 is a view similar to FIG. 2 wherein the blood, the sample tube and dispenser have been subjected to the complete centrifuging step;

FIG. 5 is a sectional view of the sample tube with the separator in place overlying the clot portion with the serum portion removed;

FIG. 6 is an enlarged sectional view of the sealant dispenser showing the sealant contained therein;

FIG. 7 is a top view of the dispenser of FIG. 6; and

FIG. 8 is a bottom view of the dispenser of FIG. 6.

DESCRIPTION OF THE ILLUSTRATED EMBODIMENT Referring to the drawings, acontainer 10 for holding a blood sample is illustrated as a straight sidewall sample tube 10 with an open top end 11. Into the open top 11 is inserted adispenser 12. Thedispenser 12 has aflange member 14 which overlies thetop edge 16 of theside wall 18 of the container. Thedispenser 12 includes a body portion 19; consisting of threering sections 20, 22 and 24. Extending from the body 19 is anelongated nozzle portion 26 and at the end of thenozzle 26 is atip 28 having an opening 30 therein. The purpose of the threerings 20, 22 and 24 is to permit thedispenser 12 to be used with containers of various diameters.Ring 20 has ashoulder 32 and aside wall 33,-ring 22 ashoulder 34 and aside wall 35, andring 24 has ashoulder 36 and aside wall 37. When using a narrow container, the dispenser bears on the top edge of the side wall of the container at one of theshoulders 32, 34 or 36 and the side wall of the next smaller ring is parallel with the inner side wall of the container. During centrifuging the relationship between the inner surface of the container and the side wall of the ring insures stability of the dispenser during this period.

By utilizing a dispenser as illustrated herein, it is possible to use one dispenser for varying diameter containers.

The body portion 19 of the dispenser has anopen end 37 over which is placed aseal 38. Theseal 38 has asmall opening 40 therein. Theseal 38 is not placed over the open end until asealant 42 has been placed in the dispenser.

Thesealant 42 consists essentially of a silicone fluid with an inert filler, such as silica, dispersed therein. The sealant should have a specific gravity of at least 1.026

. and preferably in the range of 1.030 to 1.050.

The normal specific gravity of blood as determined by the pycnometric method is considered to be in the range of 1.048 to 1.066 with averages of 1.052 to 1.063. After centrifuging the specific gravity of the blood serum which separates from the remainder of the blood is at least 1.026 and in the range of 1.026 to 1.031. The specific gravity of the heavier portions such as the erythrocytes is 1.092 to 1.095.

In selecting a sealant it is necessary to select one which has a specific gravity greater than that of the serum portion. Accordingly, the sealant should have a specific gravity of at least 1.026. However, its specific gravity should not be too high so as to cause it to layer somewhere in the clot portion. Such layering would be of no practical use towards obtaining a separated serum portion. A preferred sealant would have a specific gravity in the range of 1.030 to 1.050.

The sealant is also preferably thioxtropic, water insoluble, substantially non-toxic as well as substantially chemically inert with respect to the constituents of the blood sample, particularly those in the serum portion.

A preferred sealant formulation is as follows:

EXAMPLE I Parts by weight The silicone fluid used in Example I was a dimethylpolysiloxane polymer made by Union Carbide Corporation and identified by the designation L- 45. it had a viscosity of 12,500 centistokes and a specific gravity of about 0.973 at 25C. The silica with a specific gravity of 2.65 was an amorphous silica having a particle size of at least 75 percent being less than 5.0 microns. It was made by Whittaker, Clark & Daniels and identified by the designation No. 31 Lo Micron". The silica with a specific gravity of 2.3 was a hydrophobic amorphous silica having an average particle size of about 20 millimicrons. It was made by Degussa Inc. and identified by the designation Aerosil R 972.

The silicone fluid and the silica were mixed together to a thixotropic condition with a resultant specific gravity of 1.045 to 1.050 to form the sealant.

The sealant was then placed in a dispenser of the type illustrated in FIG. 6 in particular. The diameter of the flange was 0.70 inches and the overall length 1.75 inches with the nozzle and tip being 1.10 inches. The opening in the tip was 0.032 inches. The filled dispenser was placed in a standard sample tube having an overall length of 3.875 inches with theshoulder 32 of the first ring resting on the top of the tube side wall. The tube had previously been filled with a whole blood specimen to within 1.10 inches of the open end of the tube. The extent of the dispenser fromshoulder 32 to the end of the tip was 1.5 inches. Thus, the dispenser tip and part of the nozzle extended well into the blood sample.

The tube with the blood sample and dispenser was centrifuged for approximately 10 minutes. After 5 minutes substantially all of the sealant had passed from the dispenser. The sealant did not disperse but instead remained homogeneous and settled as a layer between the serum and clot portions of the centrifuged blood. it was noted that the sealant settled as a substantially even layer between the two portions since its specific gravity of 1.045 to 1.050 was less than that of the clot portion and greater than that of the serum portion. The sealant formed a tight seal against the inner wall of the tube. Also noted was the fact, that the sealant had mixed into it, particularly in the portion near the clot portion, fibrant matter which had been filtered out of the serum portion as the sealant settled to its own specific gravity level.

The use of the dispenser which extended into the blood sample expedited the procedure since it was not necessary for the sealant to overcome the surface tension of the blood sample.

With the sealant in place it was possible to merely decant off the serum portion with the clot being trapped behind the sealant.

The specific gravity of the sealant was determined by using a copper sulfate method. The procedure consists of letting drops of the sealant fall into a graded series of solutions of copper sulfate of known specific gravity and noting whether the drops rise or fall. The series used were graded at 0.005 intervals. Merely by observing the drops it was possible to determine that the sealant had a specific gravity between 1.045 and 1.050.

Another sealant formulation is as follows:

EXAMPLE ll Parts by weight Silicone fluid (dimethylpolysiloxane) I00 Silica (specific gravity 1.95) 14 The silicone fluid of Example 11 is the same dimethylpolysiloxane polymer of Example 1. The silica with a specific gravity of 1.05 has an average particle size of 16 millimicrons. it is made by Henlig & Co. and identified by the designation TRI-SlL 404. The specific gravity is from 1.045 to 1.050.

Still another sealant formulation is as follows:

EXAMPLE Ill Parts by weight Silicone fluid (ethyltriethoxysilane) Silica (specific gravity 2.65) 8 Silica (specific gravity 2.3) 20 The silicone fluid of Example 11 is an ethyltriethoxysilane monomer made by Union Carbide Corporation and identified by the designation A-15. The silicas are the same as those used in Example I.

The filler serves the dual purpose of making the silicone fluid thixotropic and of adjusting the specific gravity to that which is desired for the resultant sealant. In place of silica other inert fillers which may be used in a fine powdered form are bentoniate, alumina and talc. Others will also occur to those skilled in the art.

Another silicone fluid which may be used is made by Dow Corning Corporation and identified as 200 Fluid.

In selecting silicone fluids and fillers, one should select materials which when mixed together will give the desired specific gravity, be substantially non-toxic, water insoluble and substantially chemically inert with respect to the constituents of at least the serum portion.

The dispenser serves to meter out the sealant gradually and this gradual metering provides sufficient lead time for the centrifugation to take effect before the sealant is in place. If the sealant were allowed to be positioned too soon some unwanted matter, such as red cells or fibrilar like material, may be trapped in the serum portion by the sealant.

While various examples have been described herein and one embodiment illustrated on the drawings, those skilled in the art may practice the invention in its various forms by other examples and embodiments without departing from the scope of the claims herein.

What is claimed is:

1. A method of separating a sample of whole blood into serum and clot portions comprising the steps of:

placing a quantity of whole blood into a container adapted to be centrifuged;

inserting into said container a supply of a thixotropic,

water insoluble, substantially non-toxic sealant consisting essentially of a silicone fluid and an inert filler dispersed therein, said sealant having a specific gravity in the range of 1.026 to 1.092; and

centrifuging the blood into serum and clot portions until the sealant forms a'separator between said portions.

2. A method as defined in claim 1 wherein the inert filler is silica.

3. A method as defined in claim 1 wherein the sealant has a specific gravity in the range of 1.030 to 1.050.

Claims (3)

1. A method of separating a sample of whole blood into serum and clot portions comprising the steps of: placing a quantity of whole blood into a container adapted to be centrifuged; inserting into said container a supply of a thixotropic, water insoluble, substantially non-toxic sealant consisting essentially of a silicone fluid and an inert filler dispersed therein, said sealant having a specific gravity in the range of 1.026 to 1.092; and centrifuging the blood into serum and clot portions until the sealant forms a separator between said portions.

2. A method as defined in claim 1 wherein the inert filler is silica.

3. A method as defined in claim 1 wherein the sealant has a specific gravity in the range of 1.030 to 1.050.

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