US20210338121A1 - Apparatus, systems and methods for determining tissue oxygenation - Google Patents

Abstract

A surgical instrument may be configured to sense a light re-emitting probe to resolve tissue oxygenation, the surgical instrument including: an optical emitter configured to excite the light re-emitting probe within an absorption band of the light re-emitting probe; an optical detector configured to receive the re-emitted light from the probe; and a signal processor configured to resolve the tissue oxygenation based on the received light. The surgical instrument can be a surgical stapler anvil or a flexible substrate having a tissue interfacing surface. Further, a monitoring device may be configured to map oxygenation of a tissue containing a light re-emitting probe, the monitoring device including: an optical emitter configured to excite the light re-emitting probe; at least one optical detector configured to receive the re-emitted light from the probe; and a signal processor that is configured to resolve the tissue oxygenation at multiple points to generate an oxygen map.

Description

CROSS REFERENCE TO RELATED APPLICATIONS
• This application is a continuation of U.S. patent application Ser. No. 14/220,034 filed Mar. 19, 2014, which claims priority to U.S. Provisional Patent Application No. 61/803,451, filed Mar. 19, 2013, which is hereby incorporated herein by reference in its entirety.
GOVERNMENT INTEREST
• This invention was made with Government Support under National Institutes of Health grant no. CA153571. The Government has certain rights in the invention.
TECHNICAL FIELD
• The present invention relates to systems, methods and medical devices for use with molecular probes for resolving tissue oxygenation. More particularly, the inventions are directed to systems, methods and medical devices with sensors used to detect properties of biological tissue and a system for resolving the information gathered by the sensors.
BACKGROUND
• A living organism is made up of cells. Cells are the smallest structures capable of maintaining life and reproducing. Cells have differing structures to perform different tasks. A tissue is an organization of a great many similar cells with varying amounts and kinds of nonliving, intercellular substances between them. An organ is an organization of several different kinds of tissues so arranged that together they can perform a special function.
• Surgery is defined as a branch of medicine concerned with diseases requiring operative procedures.
• Although many surgical procedures are successful, there is always a chance of failure. Depending on the type of procedure these failures can result in pain, need for re-operation, extreme sickness, or death. At present there is no reliable method of predicting when a failure will occur. Most often the failure occurs after the surgical procedure has been completed. Failures of surgical procedures can take many forms. The most difficult failures to predict and avoid are those that involve biological tissue. This difficulty arises for three distinct reasons. Firstly, the properties that favor the continued function of biological tissue are very complex. Secondly, these properties are necessarily disrupted by surgical manipulation. Finally, the properties of biological tissues vary between patients.
• During a surgical operation, a variety of surgical instruments are used to manipulate biological tissues. However, traditional surgical instruments do not have the ability to obtain information from biological tissues. Obtaining information from the biological tissues that surgical instruments manipulate can provide a valuable dataset that at present is not collected. For example, this dataset can quantitatively distinguish properties of tissues that will result in success or failure when adapted to specific patient characteristics.
• What is needed are medical devices, systems and methods that can adapt to patient-specific characteristics that are of importance in avoiding surgical procedure failure.
BRIEF SUMMARY OF THE INVENTION
• A surgical instrument is configured to sense a light re-emitting probe to resolve tissue oxygenation. The surgical instrument includes at least one optical emitter that is configured to excite the light re-emitting probe within an absorption band of the light re-emitting probe; at least one optical detector configured to receive the re-emitted light from the probe; and a signal processor that is configured to resolve the tissue oxygenation based on the received light.
• The surgical instrument further includes an applicator configured to provide a target tissue with a medium, the medium containing the light re-emitting probe. The signal processor is configured to resolve the tissue oxygenation based in a lifetime of the re-emitted light. The surgical instrument is a surgical stapler anvil. The applicator is at least one injector that is configured to inject the medium into the target tissue. The surgical instrument further includes an interrogator instrument that is configured to interrogate the tissue. The surgical instrument is a flexible substrate having a tissue interfacing surface. The signal processor makes a determination of an operation success based on the resolution of the tissue oxygenation. The surgical instrument further includes a temperature sensor that is configured to detect a temperature of the tissue and at least one sensor configured to monitor interaction forces of at least one of compression pressure and tissue tension of the tissue. The probe is a phosphorescent probe that has multiple absorption wavelengths. The surgical instrument is communicatively coupled to a base station.
• A surgical stapler anvil is configured to sense a light re-emitting probe to resolve tissue oxygenation. The surgical stapler anvil includes at least one optical emitter that is configured to excite the light re-emitting probe; at least one optical detector that is configured to receive the re-emitted light from the probe; and a signal processor that is configured to resolve the tissue oxygenation based on the received light.
• The surgical stapler anvil is communicatively coupled to a base station. The signal processor makes a determination of an operation success based on the resolution of the tissue oxygenation. The surgical stapler anvil further includes a temperature sensor that is configured to detect a temperature of the tissue. The surgical stapler anvil further includes at least one sensor configured to monitor interaction forces of at least one of compression pressure and tissue tension of the tissue.
• A monitoring device is configured to sense a light re-emitting probe to resolve tissue oxygenation. The monitoring device includes a flexible substrate having a tissue interfacing surface, the tissue interfacing surface containing (1) at least one optical emitter that is configured to excite the light re-emitting probe; and (2) at least one optical detector configured to receive the re-emitted light from the probe; and a signal processor that is configured to resolve the tissue oxygenation based on the received light.
• The monitoring device includes at least one injector configured to inject a medium into target tissue, the medium containing the light re-emitting probe. The monitoring device is communicatively coupled to a base station. The signal processor makes a determination of an operation success based on the resolution of the tissue oxygenation. The monitoring device further includes a temperature sensor that is configured to detect a temperature of the tissue. The the flexible substrate is configured to be one or more of: (1) affixed to skin and (2) affixed to an internal tissue. The monitoring device is at least partially bioabsorbable.
• A monitoring device is configured to map oxygenation of a tissue containing a light re-emitting probe. The monitoring device includes at least one optical emitter that is configured to excite the light re-emitting probe; at least one optical detector configured to receive the re-emitted light from the probe; and a signal processor that is configured to resolve the tissue oxygenation at multiple points to generate an oxygen map.
• The monitoring device further includes at least one injector configured to inject a medium into target tissue, the medium containing the light re-emitting probe. The monitoring device further includes a temperature sensor that is configured to detect a temperature of the tissue. The optical detector is at least one of a CCD array, a CMOS image sensor and a camera. The monitoring device is an endoscopic instrument. The monitoring device is ingestible.
• Additional features, advantages, and embodiments of the invention are set forth or apparent from consideration of the following detailed description, drawings and claims. Moreover, it is to be understood that both the foregoing summary of the invention and the following detailed description are exemplary and intended to provide further explanation without limiting the scope of the invention as claimed.
BRIEF DESCRIPTION OF THE DRAWINGS
• FIG. 1ashows a representative embodiment of a surgical instrument with sensing capabilities.
• FIG. 1bshows a generic embodiment of a medical device with features.
• FIG. 2 shows a representative embodiment of a surgical instrument taking the form of an interrogator with sensing capabilities.
• FIG. 3 shows a representative embodiment of a minimally invasive surgical instrument taking the form of an interrogator with sensing capabilities.
• FIG. 4ashows a representative embodiment of a surgical instrument taking the form of surgical stapler anvil with sensing capabilities.
• FIGS. 4band 4cshow another representative embodiment of a surgical instrument taking the form of surgical stapler anvil with sensing capabilities integrated into the staple form.
• FIG. 4dshows a generic embodiment of a surgical instrument taking the form of surgical stapler anvil with sensing capabilities.
• FIG. 5 shows a representative embodiment of an oxygen mapping system taking the form of a minimally invasive instrument with integrated applicator.
• FIGS. 6aand 6bshow a representative embodiment of an implantable sensing device.
• FIG. 7 shows a representative embodiment of a flexible mesh or patch with integrated sensing capabilities at one or more points.
• FIG. 8 shows a representative embodiment of an applicator integrated into a surgical instrument, wherein the applicator is a micro needle array.
• FIG. 9ashows a representative embodiment of an applicator delivery system, wherein a medium is in a removable vessel.
• FIG. 9bshows three states of the injector: the initial sealed state, the punctured state, and the expended state.
• FIG. 10 shows a representative embodiment of an interrogator with capabilities of manipulating an injected probe.
• FIG. 11 shows an exemplary representation of a minimally invasive wand interrogator surgical instrument.
• FIG. 12 shows an exemplary embodiment of a medical device incorporating sensing capabilities of the present invention.
• FIG. 13ashows a representative embodiment of minimally invasive oxygen mapping system for ophthalmic surgery with an integrated applicator.
• FIG. 13bshows a representative embodiment of an oxygen mapping system as applied to retinal imaging.
DETAILED DESCRIPTION OF THE INVENTION
• Each surgical procedure has the potential for failure. A common procedure in gastrointestinal surgery is a bowel resection—removing the affected portion of the bowel and then mechanically joining the ends of the remaining segments to re-establish bowel continuity. The mechanical connection of the free ends of bowel forms what is termed a surgical anastomosis. A surgical anastomosis is formed by either traditional techniques using suture material, or by contemporary techniques which may include utilizing surgical staplers or other surgical fastening device. A surgical stapler mechanically joins the bowel by firing a pattern of staples from a cartridge or housing through the two free ends of bowel against an anvil that ultimately forms a securing crimp on the opposing side. There are many embodiments of surgical staplers. Some staplers form linear staple patterns, while others form circular patterns. Some staplers incorporate functionality for cutting tissue. Many staplers have the ability to vary the gap between the base of the staple and the formed crimp.
• Anastomotic failure is one of the most feared complications of gastrointestinal surgery due to the resultant morbidity and mortality. Failure of an anastomosis, or intestinal junction, can cause a spectrum of morbidities to the patient including local abscess formation—requiring procedural drainage, tumor recurrence, debilitating pain, dysfunctional defecation, and overwhelming bacterial sepsis resulting in death. Despite improvements in surgical technique, there remains limited ability to assess the anastomotic segment and predict outcome, and as a result anastomotic failure occurs at unacceptably high levels given the severe consequences. For example, in the performance of a low anterior resection (LAR) for excision of rectal cancer, anastomotic failure has been reported to occur in up to 30% of cases. One large multicenter, observational study of 2729 patients reported a leak rate of 14.3%. These anastomotic failures cause a significant and avoidable economic burden on the healthcare system, as well as an incalculable amount of pain, suffering, and hardship for the patients in which the failure occurs. The devastating consequences of an anastomotic failure are so severe that in the majority of cases surgeons performing a low anterior resection will opt to create a diverting ostomy at the time of resection to mitigate the risks of anastomotic failure. In the same study, 881 patients were given a temporary diverting ostomy while 128 patients developed a leak. Up to 85 percent of those patients underwent an additional surgical procedure to reverse the ostomy that provided questionable benefit. While a diverting ostomy can avoid the dire consequences of anastomotic failure, it does not prevent failure, and poses a risk for additional complications. The morbidity rate for a temporary ostomy is 20-30%. Complications include intestinal obstruction, torsion, dehydration, electrolyte imbalance, stomal retraction, and severe skin breakdown. Additionally, complications of ostomy closure occur in a third of patients. Furthermore an ostomy significantly compromises the lifestyle of the recovering patient.
• There is neither a clinically practical apparatus that can quantitatively assess an anastomosis intra-operatively to determine the likelihood of failure, nor an objective set of criteria by which a surgeon can select those patients that would benefit from diverting ostomy. Nor is there a device that can help in determining the optimal placement of an anastomosis.
• There exists a need for a device, system and methodology for reducing anastomotic failures through the analysis of target tissues before, during, and after the creation of an anastomosis. There also exists a need to objectively determine, at the time of surgery, those patients that would benefit from a diverting stoma procedure. There also exists a need to deliver adjunct therapies to the anastomotic site to optimize outcome.
• To accomplish its goal, one embodiment of the present invention interrogates the tissue, prior to creation of an anastomosis, to enable the operative team to identify a suitable site based on quantitative criteria. Another embodiment couples to the desired stapling platform, which includes traditional off-the-shelf disposable surgical staplers, and uses an array of multimodality sensors to measure the viability of the tissues during the creation of the anastomosis. If tissue parameters are unsuitable for an anastomosis, the operative team can take corrective action, thus reducing the risk of anastomotic failure. At the conclusion of the formation of the anastomosis, the operative team can ensure tissue measures remain within acceptable range. If the measures are abnormal, the operative team can again take corrective action to improve outcome. Measurements may be made from the internal and/or external surface of the tissue.
• One representative application of the present invention is in the treatment of colorectal cancer. Colorectal cancer (CRC) is the third most common cause of cancer for men and women in developed countries. Estimates predict that worldwide just under 1.2 million new cases of colorectal cancer were diagnosed in 2007. Rectal cancer accounts for approximately 27% of all colorectal cancers and presents the formidable challenge of ensuring a curative resection while maintaining acceptable function. The mainstay of treatment for rectal cancer is surgical resection—removing the affected portion of the bowel and performing an anastomosis on the ends of the remaining segments to re-establish bowel continuity. The end-to-end anastomosis (EEA) is most commonly performed using circular EEA staplers. As with any surgical procedure, resection of a rectal cancer can have complications. Amongst all of the possible complications the three most devastating to the patient in terms of morbidity and mortality are tumor recurrence, anastomotic leak and anastomotic stricture. Tumor recurrence can be reduced by: following oncologic principles of dissection, providing appropriate adjunctive chemotherapeutic, photodynamic, and radiation therapies, and preventing extra-luminal extravasation of residual intra-luminal neoplastic cells through anastomotic breakdown. Anastomotic failure has been anecdotally attributed to inadequate tissue perfusion and excessive tension at the anastomosis.
• When determining the location of a rectal cancer the surgeon notes the distance of the tumor from the anal verge. The anal canal extends from 0-4 cm past the anal verge, and the rectum 4-19 cm. Surgically the rectum extends from the anal sphincters to the sacral promontory. The location of the cancer dictates the type of surgical procedure performed.
• The primary goal of a curative resection is to remove all potential tissues harboring cancerous cells. To accomplish this goal, the surgical team aims to resect the tumor with a cancer free margin as well as the tumor's blood supply and draining lymphatic tissue. Tumors located in the upper rectum, greater than 12 cm from the anal verge, are regularly amenable to an anterior resection (AR). Those in the mid rectum, between 6-12 cm, are subject to a LAR with or without a total mesorectal excision (TME), and tumors in the lower rectum, 4-6 cm, are usually treated with an ultra-low anterior resection (ULAR), incorporating a TME, and either a colorectal or coloanal anastomosis. A total mesorectal excision is a technique that attempts to resect the rectum and all investing soft tissues en-bloc. This technique has been touted in the literature as having superior results in terms of minimizing local tumor recurrence, however it is speculated that the procedure has an inverse effect on leak rates due to the excision of the supplying vasculature to the anastomotic site. Every attempt is made to retain fecal continence, however those tumors involving the anal sphincters 0-4 cm are resected through a sphincter sacrificing abdominoperineal resection (APR). The present invention may be used to determine surgical resection margins and anastomosis location based upon sensor measurements. These measurements may be performed on the internal and/or external surface of the tissue and be interrogated as a single point or multiple locations.
• As a secondary goal the surgical team strives to restore continuity of the bowel and ensuing fecal stream. To accomplish this goal an anastomosis is formed. Simply, an anastomosis is the surgical connection of two free ends of a tubular structure. When the continuity of the bowel cannot be restored, the fecal stream is diverted through a stoma, or opening, in the anterior abdominal wall through which the patient eliminates into an ostomy bag. There are two main reasons for stoma formation: resection of the anal sphincter complex, and diversion of the fecal stream. In a sphincter sacrificing procedure such as an APR, the patient is dependent on a permanent ostomy. With a sphincter sparing procedure such as a LAR, the fecal stream may diverted through a temporary ostomy in order to mitigate the risk of overwhelming sepsis resulting from fecal contents entering the abdominal cavity should there be a leak at the anastomosis. Most of the time a temporary stoma can be reversed within a few months after the initial operation through a separate procedure. The present invention may be used to assess the viability of the anastomosis and/or stoma based upon sensor measurements.
• The scientific literature suggests that the cause of anastomotic failure is that inadequate tissue perfusion as a result of redefined vasculature, tissue interaction forces, edema, and tension result in a decrease of oxygen delivered to the anastomotic site. Without adequate oxygen delivery, efficient aerobic cell respiration cannot occur within the native cells leading to tissue degradation; collagen matrix cannot mature into strong collagen fibrils; and white blood cells cannot effectively fight bacterial invasion.
• Normal healing of a gastrointestinal anastomosis follows an orderly reparative process. The inflammatory phase occurs immediately after disruption of the tissue and lasts 2-3 days. This phase is characterized by efflux of inflammatory cells into the wound. Increased permeability of vessels adjacent to the wound facilitates the delivery of target cells. Hemostasis is achieved through a platelet mediated fibrin based clot. Neutrophils initially dominate this phase with the goal of killing invading microbes. Macrophages appear next and secrete tissue growth factors that are paramount to progressive repair. The proliferative phase begins with the arrival of fibroblasts. The fibroblasts replace the provisional clot matrix with a loosely organized collagen framework. In this phase the anastomosis first weakens as collagen lysis initially outpaces synthesis. Colonic anastomoses lose up to 70% of their initial strength. Collagen synthesis requires hydroxylation of lysine and proline residues. Cofactors essential for this process are oxygen, ferrous iron, Vitamin C, and alpha-ketoglutarate. Angiogenesis begins during this stage to increase oxygen and nutrient delivery to meet the increased metabolic demands of the healing wound. The final remodeling phase is characterized by the conversion of the relatively weak collagen from the previous phase into thick high strength collagen bundles.
• The adequate supply of oxygen is of paramount importance in each phase of wound healing. During the inflammatory phase oxygen provides neutrophils with the critical ability to kill bacteria through superoxide generation. In the proliferative and remodeling phases oxygen is a key factor in collagen formation. Likewise oxygen provides for normal aerobic metabolism of the injured tissue.
• The gastrointestinal tract, with the exception of the esophagus and lower rectum, is comprised of four layers: the outer serosa, the muscularis propria, the submucosa, and the mucosa. The submucosa consists of a layer of fibroelastic collagen matrix containing blood vessels and nerves. Halsted was the first to determine that the strength of the intestine is derived from the submucosal layer. It is the collagen fibrils within the submucosa that are mainly responsible for anastomotic integrity. Low collagen content is associated with reduced anastomotic bursting strength.
• The etiology of anastomotic failures has been attributed to a variety of local and systemic factors. Local factors include tissue hypoperfusion, anastomotic tension, poor apposition of wound edges, radiation injury, and distal obstruction. The first three local factors contributing to failure are affected by surgical manipulation of tissues. Oxygen delivery to tissues is a function of the oxygen content of blood multiplied by the blood flow rate within a volume of tissue. Blood oxygen content (BOC) of whole blood is given by the following expression:
• 
  BOC=Oxygen bound to hemoglobin+Dissolved Oxygen
• 
  BOC=1.34*Hb*SO2+0.003*PO2
• Where, 1.34 (ml/g) is the amount of oxygen functionally carried by one gram of circulating hemoglobin, Hb (g/dl) is hemoglobin, SO2 is percent hemoglobin oxygen saturation, 0.003 (ml/100 ml/mmHg) is the solubility of O2 in plasma at 37° C., and PO2 (mmHg) is the partial pressure of oxygen within the blood. Under normal physiologic conditions (Hb=15 g/dl, SO2=0.98, and PaO2=100 mmHg), BOC is 20 ml O2/100 ml arterial blood.
• As arterial blood flows through the capillary beds of the intestine, the blood cell hemoglobin desaturates as oxygen diffuses toward the supported tissue. The degree of desaturation from tissue oxygen extraction varies based on the flow of oxygenated blood through the tissue as well as the metabolic needs of the target tissue. Auto-regulation in the intestinal microcirculation allows for flow adjustment within limits responsive to demand. Under the normal flow conditions in canine ileum of 30-140 ml/min/100 g of tissue, oxygen extraction is flow-independent. The tissue is able to extract an adequate amount of oxygen to support aerobic cellular metabolism. Flow rates less than 30 ml/min/100 g oxygen extraction is flow-dependent and the tissues may not be able to support aerobic metabolism. Human small intestine has the same threshold for flow dependent oxygen. Hypovolemia and shock are contributing factors to anastomotic failure, where normovolemic anemia is tolerated if perfusion is adequate.
• Surgical manipulations of anatomy during bowel resection as well as the creation of a surgical anastomosis cause a perturbation in tissue perfusion. A canine model demonstrated a reduction in mucosal blood flow at an end to end colonic anastomosis regardless of the technique employed. However, the absolute reduction is dependent on technique. Using a circular stapler, an anastomosis formed with a staple gap equal to the measured thickness of the interposed bowel walls yielded a 43 percent reduction in blood flow, whereas a staple gap of half the measured thickness yielded a 71 percent flow reduction. A single layer sutured anastomosis resulted in a 27 percent flow reduction while a 59 percent flow reduction was recorded in a two layered sutured anastomosis. Perianastomotic serosal measurements were taken intra-operatively on both the proximal and distal aspects of the anastomosis within 1 cm of the suture line. Altered perfusion at the rectal stump correlated with subsequent anastomotic leaks. Blood flow was measured by laser Doppler at the proximal side of an end-to-end colorectal anastomosis reveals a significant reduction in flow after requisite dissection. In summary, anastomotic failure occurs when perfusion fails to provide the inputs needed to support tissue healing.
• One representative application of the present invention is in the performance of a bowel resection secondary to a bowel obstruction. Often a mechanical bowel obstruction is caused by an adhesion which causes the bowel to twist upon itself, or a hernia which incarcerates or strangulates the bowel. The blood supply to the bowel can be compromised leading to ischemia, or infarction. Intra-operatively, the surgeon qualitatively determines if the bowel is viable after it has been untwisted or freed. If the bowel does not appear viable the bowel is resected. Often qualitative methods are not accurate in determining bowel viability. If the bowel ‘pinks up’ then it may be salvageable. Questionable segments of bowel are either resected during the initial surgical procedure or left to ‘declare themselves’ over a number of hours resulting in the performance of a ‘second look’ operation to determine bowel viability. An advantage of the present invention is the ability to quantitatively assess bowel oxygenation at the time of surgery to ensure viability. If a segment is not viable and needs to be resected, the present invention can guide the surgeon in selection of a viable resection margin. The anastomosis is then performed on tissue in which normal perfusion has been confirmed.
• One representative application of the present invention is in the creation and monitoring of tissue flaps. Cancer of various types, i.e. breast, skin, etc., often cause removal of significant volumes of tissue during an attempt at curative resection. Traumatic injury may result in severed limbs or avulsed portions of tissue. The resulting tissue loss is often replaced by native tissue transposed from other parts of the patient's body. Free tissue flaps are flaps that are completely removed from their native position along with the supplying vascular pedicle. The free flap vasculature is then reconnected to vessels near the tissue void. The vascular anastomosis may fail due to leakage, stricture, or occlusion from inappropriate clot formation. The present invention enables resolution of flap oxygenation in either a point or area fashion, both for the intra-operative confirmation of tissue perfusion, and post-operative monitoring. Current technology is limited to qualitative measures of blood flow. The present invention presents real time quantitative assessment of tissue oxygenation.
• One representative application of the present invention is in the monitoring of patient oxygenation. A current method of continuously and non-invasively monitoring a patient's oxygenation is through the use of pulse oximetry. In pulse oximetry the patient's blood is interrogated with light to determine the percentage of blood that is saturated with oxygen. Unfortunately this technique is limited by technical and physiologic factors. Ambient light and motion affect the pulse oximeter's ability to render a suitable signal. Poor blood flow in critically ill patients can often render pulse oximetry useless as the pulsatile signal cannot be resolved. The present invention enables an accurate measure of tissue oxygenation independent of blood flow in a robust manner.
• One representative application of the present invention is in the monitoring of a disease state of the brain. There are numerous conditions (trauma, stroke, procedures, cancer, infection, inflammation, etc) that can affect brain perfusion/oxygenation, whether through primary means such as vessel occlusion or hemorrhage; or through secondary means by alteration of intracranial pressure. Current technology fails to provide real time measurement of direct brain oxygenation/perfusion. The present invention provides for real time measurement of brain perfusion/oxygenation through trans-cranial, intra-cranial, or extra-cranial means. The device can be positioned on an infant, over the fontanel in order to interrogate underlying brain tissue. Alternatively the device can be placed intra-cranially through a trans-cranial port, or implanted in the brain tissue to allow for continuous monitoring over extended periods of time. The device can be positioned on the eye to interrogate the internal structures such as the optic nerve, or retina.
• One representative application of the present invention is in the monitoring of organs. In this application the device can be temporarily placed or permanently implanted into or on the surface of an organ. Alternatively the device can be ingestible to monitor the gastrointestinal tract. An ingestible device could map the oxygenation, and/or other physiological parameters of the GI tract.
• The present invention includes a surgical instrument, probes, and methods for assessment of phosphorescent or fluorescent lifetime of an injectable probe or natural auto fluorescence. In one configuration, at least one sensor is configured to obtain biological tissue oxygenation utilizing the technique of oxygen dependent quenching of phosphorescence of an injectable probe. In another embodiment, the present invention measures lifetime of a marker or other probe in or on the body. In a further embodiment, lifetime of phosphorescence or fluorescence produced from native biologic tissue is assessed.
• Other potential applications include but are not limited to the monitoring/recording of a transplanted organ or appendage, intra-cranial, intra-thecal, intra-ocular, intra-otic, intra-nasal, intra-sinusoidal, intra-pharyngeal, intra-laryngeal, intra-esophageal, intra-tracheal, intra-thoracic, intra-bronchial, intra-pericardial, intra-cardiac, intra-vascular, intra-abdominal, intra-gastric, intra-cholecystic, intra-enteric, intra-colonic, intra-rectal, intra-cystic, intra-ureteral, intra-uterine, intra-vaginal, intra-scrotal; intra-cerebral, intra-pulmonic, intra-hepatic, intra-pancreatic, intra-renal, intra-adrenal, intra-lienal, intra-ovarian, intra-testicular, intra-penal, intra-muscular, intra-osseous, and intra-dermal physiologic/biomechanical parameters.
• The present invention relates to medical devices capable of measuring physiologic and mechanical properties of tissue. One embodiment of the invention relates to coupling sensing elements with a surgical instrument to determine tissue viability during surgery by measuring the optical time response from a light re-emitting medium. In a specific embodiment, an oxygen-sensitive phosphorescent oxygen sensing probe is injected into tissue, and a laparoscopic interrogator uses optical sensing elements to determine tissue oxygenation based on the optical response (i.e. lifetime) of light emitted by the phosphorescent probe.
• The sensing elements associated with the invention may sense mechanical or biological properties. The sensing instruments may include one or more sensing modalities. The sensing modalities may include mechanical, optical, chemical, electrical, or other means for generating a signal indicative of a property of a subject tissue. In one embodiment, the sensing elements measure oxygenation through the use of a medium containing a phosphorescent probe or phosphor delivered into tissue. Other embodiments measure oxygenation through oximetry-based techniques. Further embodiments measure perfusion or flow rates through the time response of a fluorescent or phosphorescent medium introduced into the tissue.
• In an embodiment, the sensing elements are incorporated into, or coupled to, the working surface (i.e. tissue contacting surface) of a surgical instrument for manipulating biological tissue. Instruments may include traditional open, laparoscopic, endoscopic, bronchoscopic, otoscopic, opthalmoscopic, laryngoscopic, cystoscopic, colposcopic, intravascular, intraluminal, robotic, or other minimally invasive tools such as a purpose-built tissue interrogator or instrumented standard instrument such as a grasper, needle driver, stapler, clip applier, catheter, scissor, cautery, or retractor. Instruments may also include interrogators or other devices that may or may not be minimally invasive and may interrogate the internal and/or the external surface of tissue.
• The sensing elements may include mechanical and optical sensing modalities. Mechanical sensing includes, but is not limited to, pressure sensors that monitor tissue interaction forces including compression pressure and tissue tension. Optical sensing elements include but are not limited to light emitters including light emitting diodes (LEDs), broad band light sources, and laser diodes (LDs), and light receivers including photodiodes (PDs) and silicon photomultipliers (SiPMs), CCD arrays, CMOS imaging sensors, cameras, and spectrometers. The sensors may make measurements at a single discrete location or at a plethora of locations. The optical sensing elements are configured to measure at least one of tissue oxygenation, oxygen delivery, oxygen utilization, tissue characterization, and tissue general health using oximetry, phosphorescent techniques, or spectroscopic techniques, and at least one of tissue perfusion, tissue flow dynamics, tissue oxygen content, tissue chemical composition, tissue immunologic activity, tissue pathogen concentration, or tissue water content using fluorescence or phosphorescence based techniques. The fluorescence and phosphorescence based techniques include but are not limited to the following: monitoring and analyzing the intensity and time course of a fluorescent or phosphorescent response responsive to the injection or activation of a fluorescent or phosphorescent medium (e.g., fluorescein or indocyanine green injection), determining oxygen quantities by measuring oxygen-dependent quenching of fluorescent or phosphorescent radiation using a sensitive material (e.g., ruthenium) by both intensity and time-resolved methods, determining oxygen concentration based on the quenching time response (lifetime) of injectable oxygen sensitive phosphorescent probes (e.g., oxygen-dependent quenching phosphorescent nano sensors), and determining the target tissue property by quantitative fluorescent or phosphorescent methods (including the use of quantum dots, or other biomarkers incorporating light re-emitting properties). In one configuration the device senses perfusion using flourescein, or IC green, or other imaging agent. In one other configuration the device senses native oxygen quenching or phosphorescence.
• Measurement of tissue oxygenation or other tissue characteristics can be measured in a gated fashion to standardize the measurement and allow for comparison. One representative example of said gated measurement is the measurement of tissue oxygenation at a known interaction force(s) such as compression pressure. Measurements may also be gated to physiologic parameters such as respiration and cardiac output.
• PCT Patent Application No. PCT/US2006/013985, which is hereby incorporated herein by reference in its entirety, describes a system and methodology for using the information gathered by surgical instruments having sensors in an adaptive, patient-specific manner. The present invention can be used to predict outcome, the likelihood of success or of failure, or guide surgical procedure, and as described in PCT/US2006/013985, US Patent Application Publication No. 2012/0116185, which is hereby incorporated herein by reference in its entirety, and U.S. Pat. No. 8,118,206, which is hereby incorporated herein by reference in its entirety. Furthermore the present invention can be configured as an adjunct sensing system, and as described in US Patent Application Publication No. 2012/0116185 and U.S. Pat. No. 8,118,206. Additionally, the present invention can be powered by radiofrequency techniques, and as described in US Patent Application Publication No. 2010/0081895, which is hereby incorporated herein by reference in its entirety.
• Tissue parameters can be measured by a variety of methods. One technique utilized by the present invention measures tissue oxygenation levels via utilizing oxygen dependent quenching of phosphorescence via a systemic or locally injected phosphorescent oxygen sensing molecular probe for oxygen measurements. See, for example, U.S. Pat. No. 4,947,850, which is hereby incorporated herein by reference in its entirety, U.S. Pat. No. 5,837,865, which is hereby incorporated herein by reference in its entirety, U.S. Pat. No. 6,362,175, which is hereby incorporated herein by reference in its entirety, U.S. Pat. No. 6,165,741, which is hereby incorporated herein by reference in its entirety, U.S. Pat. No. 6,274,086, which is hereby incorporated herein by reference in its entirety, U.S. Pat. No. 7,575,890, which is hereby incorporated herein by reference in its entirety, and US Patent Application Publication No. 2013/0224874, which is hereby incorporated herein by reference in its entirety. The phosphorescent oxygen sensing probe can include a phosphorescent metalloporphyrin core encapsulated inside hydrophobic dendrimers, which form a protecting shell that isolates the chromophore from direct contact with the environment, controls oxygen diffusion, and enables control over the probe's dynamic range and sensitivity. The metalloporphyrin core can be constructed with different elements. Palladium (Pd) and platinum (Pt) are two elements that can be utilized. The advantage of a platinum based core over a palladium based core is its quantum efficiency. The increase in the quantum efficiency of the phosphor allows for a significant increase of light output when compared to the Pd based molecule; more light returned per molecule allows for the use of fewer molecules to achieve the same signal returned to the device. Alternatively injection of the same amount of molecule enables the use of less sensitive (less expensive) photo-detectors. Peripheral PEGylation of the dendritic branches ensures high aqueous solubility of the probe whilst preventing interactions with biological macromolecules. The overall size of the molecular probe affects the probe's ability to be cleared by the kidney. Faster clearance limits the agent's exposure to the patient. The size can be varied through the modification of the dendrimer length, number of dendrimers, and the extent of PEGylation.
• In one embodiment of the probe, the core, Pd-meso-tetra-(3,5-dicarboxvphenyl)tetrabenzoporphyrin (PdTBP), is encapsulated by eight generation2 poly-arylglycine (AG2) dendrons; each of which are PEGylated with monomethoxy-polyethyleneglycol amine (PEG-NH2) groups (Av. MW 1,000 Da), having on average 21-22 monomeric —(CH2CH2O)— units. The molecular weight of the probe dendrimer was found to be in the range of −26,000-44,000 Da with a maxima of 35,354 Da as determined by MALDI mass spectroscopy. The phosphorescence quenching method relies on the ability of molecular oxygen (O2) to quench phosphorescence of excited triplet state molecules in the environment. In biological systems phosphorescence quenching by oxygen occurs in a diffusion controlled fashion and is highly specific to O2, since O2 is the only small-molecule dynamic quencher present in sufficiently high concentrations. The dependence of the phosphorescence lifetime (t) on the partial pressure of oxygen (pO2) through the range of biological concentrations is well described by the Stern-Volmer equation: 1/τ=1/τ0+kq×pO2, where T is the phosphorescence lifetime at a specified oxygen pressure pO2, τ0 is the phosphorescence lifetime in the absence of oxygen (pO2=0), and kq is the quenching constant. One molecular oxygen probe has a quenching constant, kq, of approximately 326 mmHg⁻¹s⁻¹, and a τ0 of 210 μs over the range of physiologic pH, 6.2-7.8, and constant temperature of 36.5° C. The calibration parameters of said probe, kq and τ0, change linearly with respect to temperature. The quenching constant, kq, increases from 211 mm Hg⁻¹s⁻¹to 338 mmHg⁻¹s⁻¹with the rise of temperature from 22° C. to 38° C., which corresponds to the temperature coefficient of 7.8 mm Hg⁻¹s⁻¹/° C. The absorption spectrum of said probe has maxima at approximately 448 nm and 637 nm with a phosphorescence emission maximum of 813 nm. Excitation at multiple wavelengths (either separately or simultaneously) confers an application specific advantage of being able to interrogate and distinguish tissue properties at differing penetration depths or layers. A combination of multiple pO2 values in a field of view (e.g., where different tissue layers are differently oxygenated) will manifest itself as a combination of lifetimes (a sum of exponential decays); multiple pO2 values and corresponding concentrations can be determined through means described herein.
• FIG. 1ashows a representative system with sensing capabilities according to an embodiment of the present invention. This embodiment specifically depicts asurgical instrument101, wherein the instrument is a minimally invasive device for manipulating biological tissue. The instrument may be designed for open, laparoscopic, endoscopic, or other surgical approaches as previously noted. The instrument interacts with tissue ofbody103. In one embodiment, said tissue isintestinal tissue107. One configuration ofinstrument101 incorporatesjaws109 and111 to interact withtissue107. In one embodiment, one ormore jaws109 and111 contain sensors or sensingelements110 and112 for measuring biological or mechanical properties oftissue107. In a further embodiment, the jaws are configured to ensure constant compression pressure oftissue107 against sensing elements injaws109 and/or111. One or more jaws may contain an inflatable cavity, bladder, balloon, catheter, compression plate, orpressure sensor113 for compressing tissue against one or more sensing elements.Jaws109 and111 may incorporate control electronics and sensing elements.Jaws109 and111 are coupled toinstrument101 and may either be a fixed distance apart, be able to move in a parallel motion, or move in a hinged motion. In one embodiment, the distal tissue contacting end of theinstrument101 is articulated at a joint114 with one or more degrees of freedom.
• In one embodiment,jaws109 and/or111 contain an applicator capable of delivering a medium intotissue107. The medium may incorporate a fluorescent or phosphorescent oxygen sensing molecular probe. In one embodiment, the applicator comprises one or more microneedles incorporated into one ofjaws109 and111. Said microneedles may take the form of a molded needle array. In another embodiment, one of more ofjaws109 and111 incorporates needles with a porous surface for delivering the medium intotissue107. In another embodiment the needles have at least one side hole, and an occluded tip. One embodiment of the present invention incorporates an integrated injection system. The injection system includes avessel117 that contains the medium118. The medium118 that has been introduced intotissue107 is identified as119. In one embodiment, thevessel117 contains a number of metered doses of a medium118 comprising the phosphorescent oxygen sensing molecular probe.Vessel117 is coupled with the one or more needles associated withjaws109 and/or111.
• In one embodiment,instrument101 contains a handle orother component121 that encloses one or more of control electronics, battery power sources, user interfaces, displays, and a wired or wireless communications interface. Theinstrument101 orcomponent121 may include controls for adjusting compression pressure betweenjaws109 and111, articulating joint113, administering injections fromvessel117, and operating sensing elements located injaws109 and/or111.Component121 may be optionally detachable frominstrument101, allowing for a partially disposable configuration of101, in which121 is reusable. The control electronics in121 or other location oninstrument101 may communicate with anexternal interface125 through a wireless orwired connection123. The external interface may take the form of a free standing base station, a computer, a tablet style computing device or other portable electronic device with a display, or other device.External interface125 may additionally be connected to an external network.Device125 displays information to the clinician ondisplay127. This information may include tissue oxygenation, blood flow, maps of tissue oxygenation, compression pressure, warning information, predictions about the likelihood of success of the procedure, or other physiologic, mechanical, patient status, or situational information. Sensory substitution including audio, visual, vibration, or other techniques may be provided byinstrument101 orexternal device125.
• FIG. 1bshows further detail of a representative medical device with sensing capabilities according to an embodiment of the present invention. Abiological tissue151 contains a medium153. In one embodiment of the invention, medium153 contains a phosphorescent oxygen sensing molecular probe injected intotissue151. The medium may be introduced systemically or may be injected locally. In one embodiment, one or more needles ordelivery cannulas157 with at least oneorifice159 are used to locally injectmedium153. Amedical device155 is configured to interrogatetissue151.Device155 may also comprise an applicator for delivery of a medium. In one configuration,needle157 is a microneedle andorifice159 is one or more holes on the lateral side of said needle. A vessel orsyringe163 contains medium165; medium165 is the same material asmedium153 prior to injection intotissue151. Aninjector167 may be used to inject medium165 intotissue151 as represented by medium153 in the tissue.Injector167 may be manually controlled or may be motorized or otherwise actuated.Injector167 may be configured to provide metered doses ofmedium165.Vessel163 andinjector167 may be an integral part ofmedical device155, may be an accessory tomedical device155, or may be configured as an independent injection system.Medical device155 contains at least onesensor171, which further contains sensingelements173 and185. In one embodiment, sensingelement173 is a light emitter source that emits light175 that optionally passes through anoptical filter177 into the medium153 oftissue151. Uponincident light175, medium153 re-emits anoptical response light181.Optical response181 optionally passes through anoptical filter183 to anoptical detector185. Afurther sensor187 may provide a measurement of contact or of pressure between a surface ofinstrument155 andtissue151. Components ofsensor171 are coupled withprocessor189 that controlslight source173 and acquires signals fromdetector185.Processor189 controls light emission from173 and processes signals from185 so as to perform a sensing operation. In one embodiment, acommunication interface191 communicates with an external base station orother device195 through alink197.Link197 may be a wireless communication betweendevice155 and base station ordisplay195.
• In one embodiment of the present invention, medium153 and165 contains fluorescent or phosphorescent oxygen sensing molecular probe.Light source173 may be a narrow band light source such as an LED or laser, or may be a broadband source such as a white light source. The peak emission wavelength of the narrowband source is selected to be at or near an absorption peak of the probe (e.g., an excitation wavelength of the probe) inmedium153.Filter177 may be used to further restrict incident light175 to wavelengths in or near the absorption wavelength region of the probe. The probe re-emits light181 which then optionally passes throughfilter183 to isolate theemission light181 from theincident light175.Light detector185 senses the intensity of received light181. In one configuration,detector185 is a single point detector such as a PD, APD, SiPM, or similar device. In an alternate configuration,detector185 is a multi-point detector such as a camera or an array of single point detectors. The camera may be CCD, CMOS, or other technology and may be directly at the tissue contacting surface ofinstrument155 or optically coupled at a remote location. The array of single point detectors may be PD array, SiPM array, linear CCD or other technology. In one configuration,processor189 commands light pulses fromsource173 and analyzes the time response of the signal received bydetector185 using time domain signal processing techniques. In an alternate configuration,processor189 commands modulated light such as a sinusoidal intensity profile fromsource173 and analyzes the measured signal fromdetector185 to determine the phase lag through frequency domain signal processing techniques. In one configuration the probe inmedium153 quenches the lifetime of the phosphorescent re-emission in response to oxygen in the vicinity of the probe. This relationship between oxygenation and phosphorescent lifetime may follow the Stern-Volmer relationship. Time domain or frequency domain techniques may be used by thesignal processor189 to quantitatively resolve the corresponding oxygen content or concentration in a location oftissue151. By resolve it is meant to calculate, compute, determine, assess, or acquire the solution for oxygen content or concentration in the target tissue. An exemplary implementation of said time domain or frequency domain techniques is taught in U.S. Pat. No. 6,707,168-B1. Oxygen content may be represented as a number or shown as a map of oxygenation on eitherdevice155 or anexternal display unit195. The oxygen content may be used to predict the likelihood of success or failure of the surgical procedure, or guide a surgical procedure. An exemplary implementation of predictive or guidance techniques is taught in US 2009/0054908 A1, which is hereby incorporated herein by reference in its entirety. In one embodiment,instrument155 is a minimally invasive surgical instrument. In another embodiment,device155 is an anvil of a surgical stapler. In another embodiment,device155 is an adjunct to a surgical instrument, such as an accessory to a surgical stapler anvil. In another embodiment,device155 is an internally implantable sensor. And in another embodiment,medical device155 is an externally wearable device such as a patch or is an implantable sensor.
• In one configuration of the present invention, the medical device (such as a surgical instrument)101 or155 or other embodiments are configured to sense oxygenation in multi-layered tissue, or to discriminate oxygenation at different depths of tissue. Using a phosphorescent oxygen sensing probe having multiple absorption wavelengths inmedium119 or153, theinstrument101 or155 can irradiate and excite a subset of the probe injected intotissue107 or151 based on the excitation wavelength emitted fromdevice101 or155 since the penetration depth in tissue is wavelength-dependent. Oxygenation can be discriminated at two or more depths or layers by exciting the tissue sequentially with multiple excitation wavelengths at or near absorption peaks of the probe, and determining the corresponding quenching response. Sensing the deeper values will be a summation of multiple layers, oxygenation at deeper layers can be determined by accounting for the sensed oxygen at shallower layers. In an alternative approach, the phosphorescent decay of various oxygenation levels in heterogeneous luminescence systems (i.e. mixed oxygenations within the tissue sample) can be determined through deconvolution methods to produce a spectrum of oxygenation.
• In one embodiment of a sensing medical device, a plethora of sinusoidally modulated excitation light outputs are generated (either simultaneously, separately, or combined into a time varying frequency signal such as a chirp) and frequency domain techniques are utilized to determine the spectrum of phase lag of the received signal from an injected phosphorescent medium. By determining the relative contributions of each phase lag, a quantitative spectrum of tissue oxygenation may be generated. In another embodiment, time domain techniques are utilized to determine the time response of the medium to a pulse of light. Multiple exponential fitting of the decay can be used to generate a quantitative spectrum of tissue oxygenation.
• FIG. 2 shows a tissue interrogator. Theinterrogator201 evaluates properties oftissue203. In one embodiment,jaws205grasp tissue203. A compression control device such asscrew207 or a compression device (e.g., a balloon or compression plate) affixed tojaws205 regulate pressure ontissue203. Sensingelements211 are integrated into the one or more tissue contacting surfaces. In one embodiment, an injection system is incorporated into acavity213 of the device. Needles incavity213 are used to deliver a medium205 intotissue203. In one embodiment, a phosphorescentoxygen sensing probe205 is injected intotissue203. Sensingelements211 obtain data to measure tissue oxygenation by illuminating tissue with a light source and assessing the time response of the re-emitted light (i.e. phosphorescent quenching time response).Control electronics215 operate thesensing elements211 and are communicatively coupled through221 tocommunication interface223.Interface223 is coupled through225 to an external controller oruser interface227.
• FIG. 3 shows one embodiment of a wand-type tissue interrogator designed for minimally invasive surgical interventions.Wand301 containslower jaw303 andupper jaw305 to interact with (i.e. enclose) a biological tissue.Lower jaw303 contains acompression device309 to standardize and maintain consistent compression pressure of the tissue.Upper jaw305 containscontrol electronics311 and sensing elements on the tissue contacting surface. In one embodiment,jaws303 and/or305 contain an injection system for introducing a medium into the tissue. This medium may include a phosphorescent oxygen sensing probe or another agent capable of imaging, diagnostic, or therapeutic purposes. Theapplicator injection system317 may be incorporated into the handle or body ofinstrument301. This may include a vessel containing one or more doses of the medium. A user can administer the dose precisely from theinstrument301 to needles injaws303 and/or305. The needle may be microneedles that may further take the form of a micromolded needle array. They may also take the form of porous needles that saturate the tissue in the region for microinjection of the medium into the tissue in contact with the instrument jaws.Jaws305 and303 may be fixed relative to each other, or able to open and close. The distal end of the instrument may be articulated at joint319. In one embodiment,instrument301 fits through a standard laparoscopic surgical instrument port and articulates at joint319 to positionjaws303 and305 around a tissue. In one configuration, atube321 is used to inflate or otherwise controlcompression device309. In a further configuration,compression device309 is a balloon catheter. In another embodiment, atube321 is used to deliver the medium for injection into the tissue. In a further embodiment, the sensing instrument is connected via a wired or wireless connection to an external interface. In one configuration, the instrument interacts with intestinal or colonic tissue and measures tissue oxygenation at or near the site of a surgical anastomosis. The instrument may take measurements before, during, and after the anastomosis is created. It may be used as a stand-alone device or in combination with an additional sensing instrument such as a sensing anvil for a surgical stapler. This configuration or another embodiment of an interrogator may be used to take measurements on the internal and/or external surface of tissue. It may take measurements at a single location or at a plethora of locations.
• FIG. 4ashows a representative embodiment of ananvil401 for a surgical stapler instrument with sensing capabilities. Workingsurface403 makes contact with the biological tissue. Sensingelements405 make contact with tissue at workingsurface403. The sensing elements may be an integral part ofanvil401 or an accessory. In one configuration, the sensing elements are integrated into anaccessory409 that couples to the anvil. The present invention includes sensing elements both directly integrated into and also otherwise associated with the surgical instrument (i.e. an accessory or adjunct device).FIG. 4bshows one representative configuration of the present invention with sensing elements incorporated intocutouts422 in the face of working surface of a modifiedsurgical stapler anvil420. In one embodiment,anvil420 contains a sensorelement interface circuit426 couples via a flexible cable to acontrol circuit430.Control circuit430 may contain a processor for controlling and interpreting sensor elements. The control circuit may communicate via awireless transceiver432. The device is powered via abattery436. All components are contained within a shell, cap orcover438.FIG. 4cfurther details one embodiment where optical446 and mechanical sensing elements are integrated into the cavities inface441 of the anvil betweenstaple forms442.444 represents a mechanical sensor such as a pressure transducer die that measures compression pressure onsurface442 of the anvil.445 represents an integrated temperature sensor.446 and447 represent optical sensor elements, where446 are one or more light emitters and447 is a photodetector. In one embodiment the optical sensor elements include multiple light emitters at more than one peak wavelength so as to enable depth-resolved sensing. Sensor elements444-447 may be located in the cavities in various configurations including combined together or distributed across thesurface441. Acover lip448 encloses the edge of the sensor elements. In one embodiment, the sensing components are incorporated into a shell that couples to a surgical stapler anvil, or alternatively to the stapler body or other component. In another embodiment, the sensing components are incorporated directly into an anvil for a surgical stapler and replace a traditional non-sensing anvil. In both cases, the sensing shell or replacement anvil serve as an accessory or adjunct to the surgical stapler and may be optionally coupled to the stapler. The sensing anvil may be used as a stand-alone device or in combination with an additional instrument such as an interrogator.
• FIG. 4dshows a representative embodiment of asurgical stapler anvil450 with sensing capabilities according to the present invention.Anvil450contacts tissue452 and compressestissue452 against asurgical stapler housing454.Anvil450 is coupled tohousing454 via astalk456. Surgical staple forms are integrated into workingsurface460 of thesurgical stapler anvil460. In one embodiment of the invention, one or more sensors orsensor elements464 are incorporated into cavities formed in the workingsurface460 between staple forms. In one embodiment, sensors orsensor elements464 are configured to measure oxygenation oftissue452 by interrogating a probe inmedium468 which has been injected into said tissue. In one embodiment, medium468 contains a phosphorescent probe whose lifetime is indicative of oxygen content andsensor elements464 comprise light emitters and detectors. Sensors orsensor elements464 may also comprise pressure sensors for detecting contact or compression pressure ontissue452. Sensors orsensor elements464 may also comprise temperature sensors and/or pH sensors. Sensors orsensor elements464 are coupled to a processor orsignal processor470. In one embodiment,processor470 controls one or more light emitters, receives signal from one or more light detectors, and determines oxygenation responsive to said signals.Processor470 may also utilize pressure sensor data for gating a reading to a predefined pressure to ensure consistent readings.Processor470 may also utilize temperature and/or pH sensor data for calibrating the oxygenation measurement. In one embodiment, anapplicator474 is coupled withanvil450.Applicator450 may contain a medium476 or be coupled to a syringe or other vessel containing the medium.Applicator474 may contain one or more needles or amicroneedle array478 to inject the medium into thetissue452 as represented by468.Applicator474 may be a standalone device or integral toanvil450.Applicator474 may be keyed toanvil450 so as to delivery a microinjection at the location ofsensor elements464.
• In one embodiment of the present invention, the surgical instrument contains LEDs, lasers, or other light sources on the working surface of the surgical instrument that introduce excitation light into a phosphorescent or fluorescent medium delivered into a tissue in contact with said surface. Re-emitted light (i.e. fluorescent or phosphorescent emission) is sensed by PDs, avalanche photodiodes (APDs), photo-multiplier tubes (PMTs), SiPMs, or other photodetectors. Control electronics measure the time response of the oxygen-dependent quenching of the phosphorescent response and said information is used to determine tissue oxygenation. Time domain or frequency domain based techniques may be used to determine tissue oxygenation. In one embodiment, the medium incorporating phosphorescent oxygen sensing probes may be introduced using an injector integral to the surgical instrument. Microneedle, needle arrays, surface absorption, or other approaches may be used to introduce the medium. An injection system may be incorporated into the surgical instrument. In another configuration, a separate injection system is utilized which may be by traditional means (i.e. needle and syringe) or a stand-alone injector specifically designed to operate with the instrument. In one configuration, a stand-alone injector is keyed to the sensing anvil to align injection locations with sensor locations. These techniques may be applied to a laparoscopic interrogator, a stapler, a laparoscopic or other minimally invasive instrument, a robotic instrument, or an instrument for open surgery.
• FIG. 5 shows an oxygen imaging system for minimally invasive surgery.Instrument501 entersbody503 through a standard port of laparoscopic or endoscopic surgery or an existing lumen of the body. The instrument interrogatestissue505.Tissue505 has aregion509 where a medium containing a fluorescent or phosphorescent oxygen sensing probe has been introduced. In one embodiment, aninjector511 is incorporated into or coupled into theinstrument501. In an alternative embodiment, other methods of local injection or systemic bolus injection may be used.Instrument501 incorporates animaging system513 at the distal end. Theimaging system513 illuminates the probe inregion509 and detects the oxygen-dependent quenching of the phosphorescent response. In one embodiment,imaging system513 incorporates a camera and the system provides a2D map of tissue oxygenation. Theimaging system513 may take on multiple embodiments which may comprise a standard camera (such as CCD or CMOS), an intensified camera system, linear array, or scanning sensor.Imaging system tip513 may directly contain the camera, or it may be fiber optically coupled to a remote camera system. The map may appear as a quantitative image of tissue oxygenation, and may be further visualized in combination with traditional endoscopic video images. In a further embodiment, a3D map conforming to the tissue shape is generated. The3D map may be generated by registering multiple images from a single camera (such as using shape from motion techniques) or from multiple cameras (such as a stereo camera). One embodiment of the invention is aminiature sensing instrument501 for assessing oxygenation inside of the eye. The instrument may measure oxygenation at a single location or by generating a2D or3D quantitative map of oxygenation. To minimize the tip of the miniature sensing instrument, optical fibers or other light guides couple theimaging system tip513 to a remote detector. The system can detect and map areas of high and/or low oxygen or blood flow.
• FIG. 6 shows a configuration where a circular surgical stapler delivers asensing device605 on its workingsurface607. The sensing device places a plethora of optical fibers around ananastomosis609.Fiber bundle611 passes through theanus615 tointerrogator617. In one embodiment, the interrogator is configured to assess the phosphorescent lifetime of oxygen-dependent quenching of an injectable oxygen sensing probe. In a further embodiment, thesensing device605 is bioabsorbable and the optical fibers can be readily removed at a time after the surgical procedure.
• FIG. 7 shows a configuration of the invention where sensing elements are incorporated into aflexible substrate701. One ormore sensing elements705 are in contact withtissue703. A medium containing phosphorescent oxygen sensing probes is introduced intotissue703 and sensingelements705 illuminate the tissue and monitor the oxygen-dependent quenching of the phosphorescent response. The device may take the form of a patch. It may be applied internally or externally. The patch may be at least partially bioabsorbable. The invention may include an integrated injection system or transdermal delivery system for the sensing medium. One configuration of the patch is for skin flap monitoring or organ transplant assessment. A further configuration is an externally wearable patch to reliably provide tissue oxygenation in a clinical environment that is accurate across a wide range of oxygenations and robust to motion artifact and other sources of error present in pulse oximetry or other traditionally used techniques.
• In one embodiment of the invention, the phosphorescent oxygen sensing molecular probes are coupled to a ferromagnetic material andsubstrate701 contains an apparatus for manipulating the probe location, or clearance rate. In one configuration, magnets including permanent, switched permanent, or electromagnets are integrated into or otherwise coupled tosubstrate701. The magnets attract the probes in the medium to ensure that they remain in thetissue703 within range of thesensing elements705. In a further embodiment, the probe is configured with a size appropriate to maintain its location in the tissue for a period of time. The size of the probe is configured such that it remains intissue703 without migrating such that sensing may be performed without the need for multiple or continuous injection. The probe may be sized such that it does not migrate, that it clears naturally after a delay, or that it clears rapidly. In a further embodiment, bioabsorbable components are coupled with the probe, such that the probe will have minimal migration while the bioabsorbable components are in place. After a period of time the bioabsorbable material no longer impedes migration or clearing of the probe and it can clear from thetissue703 and the body. The above described techniques are not limited to use with the embodiment of the patch shown inFIG. 7 and may be applied to other embodiments.
• FIG. 8 shows a microinjection system integrated intosurgical instrument801.Needles803 protrude fromsurface805 into a tissue. In one embodiment, needles803 are controlled so as to only protrudepast surface805 upon reaching a specific tissue compression pressure.Needle803 may be a microneedle array. The array may be micromolded. In another configuration, needles803 are porous needles that deliver the medium along their length into the tissue. In one embodiment, an injection system andvessel809 are contained in the body ofinstrument801 and connected through atube811 toneedles803. In an alternate embodiment, a bladder or cavity is collocated with the needle or needle array and used to deliver the medium. In one configuration of theneedles803 are arranged in along a line (straight or curved), and sensing elements are placed along one or both sides of the needles.
• FIG. 9adetails one embodiment of an injection/applicator subsystem configured to deliver medium from apparatus into target biological tissue. A sealedvessel901 containing medium with light re-emittingmolecular probe903 is incorporated into the body of asurgical instrument905. The vessel is comprises anouter shell909, and aplunger911. Theplunger911 is configured to mate withneedle carrier915. The surgical instrument has a vessel receiver comprising abody919, and aneedle subunit921. Thebody919 ofvessel receiver921 receives theouter shell909 of thevessel901. Theneedle subunit921 comprises abody925, ahollow needle927, and abreakable stop929. Theneedle body925 receives theplunger911. Theinitial position931 of theplunger911 withinneedle unit body925 abuts up againstbreakable stop929. Theneedle927 is configured such that during initial positioning of the plunger against the breakable stop, the needle is embedded in the plunger, not in contact with the medium. The other end ofneedle927 is coupled to a tubing ormanifold949 which conveys medium903 to needle(s)956. The user can inject discrete amounts ofmedium905 into thetissue954 through needle(s)956 at the instrumentstissue contacting surface958 by manipulatingknob935.Knob935 comprises aratcheting mechanism937 allowing for rotation in one direction, at discrete increments. The knob is fixed toactuator rod939.Rod939 extends from knob intosurgical instrument body905. The distal end of the rod holds apin941. Thepin941 mates with constant orvariable pitch threads943 inpusher unit945. Thepusher unit945 abuts againstvessel shell901. To inject the medium903 into thetissue954, theknob935 is rotated, which turnsrod939. The subsequent rotation ofpin941 advances thepusher unit945, andvessel901 into thevessel receiver921. Initial rotation of theknob935 advances the plunger past thebreakable stop929, tofinal position933, resulting in communication ofneedle927 withmedium903 and priming of needle(s)956 with medium. Further rotation ofknob935 results in discrete advancement ofvessel901 and ejection of the medium903 from thevessel901 in one or more controlled doses shows intissue954 as950. Anoptional indicator947 can be incorporated intosurgical instrument905 to indicate state of actuation and dose number. Actuation of the injection system may be either manual or electronically actuated. The medium903 may be contained in aprefilled vial901 in the form of a solution or it may be packaged in dried lyophilized form and reconstituted at the time of use.FIG. 9bshows three states of the injector: the initial sealed state, the punctured state, and the expended state.
• FIG. 10 shows aninstrument1001 insidebody1003, interrogatingtissue1007. Sensing elements are integrated intojaw1009. A medium is injected intotissue1007. The medium is ferromagnetic. In one configuration, magnetic components are placed in the tip ofinstrument1001 alongjaw1009 to control the flow of the medium. In one configuration, a medium is injected systemically or locally, andmagnets1013 attract the medium or component therein to the sensing elements or slow the flow away from the sensing elements. In another configuration the magnetic components are controllable to manipulate the medium, or particles therein. The magnetic field may be manipulated by moving permanent magnets, switching electro-permanent magnets, adjusting power to electromagnets, or controlling the relative position of the jaws to the tissue and/or medium in the tissue. The magnetically controllable particles or medium may be used in sensing applications, or may be for delivering therapy.
• In a configuration of the invention, the probe is coupled with ferromagnetic material such that it is possible to control its delivery via magnetic forces. In another configuration, the probe is coupled with bioabsorbable entities that protrude from the surface. Said entities allow the locally injected probe to remain at or near the injection site for a period of time. After which time, the bioabsorbable entities will dissolve and the probe will be free to clear from the body.
• FIG. 11 shows an exemplary representation of a minimally invasive wand interrogator instrument. Theinstrument1101 has a tissue contactingdistal end1103. In one configuration, thedistal end1103 is articulated with respect to the main body ofinstrument1101 at joint1107. Joint1107 may have one or more degrees or freedom of articulation. The articulation is controlled from anactuator1109 on the body of the instrument.Actuator1109 may be a slider, a knob, a motorized or robotic motion, or an alternative means of controlling the articulation of joint1107. Joint1107 may take the form of a rigid hinge or pivot joint or a series of hinged pivots. Alternatively, joint1107 may be compliant in one or more degrees of freedom. In one configuration, joint1107 is a flexure joint actuated using shape memory alloy (SMA) or other flexible cables or rods that are controlled by moving ahandle1109.
• Distal end1103 contains two tissue contacting components,upper jaws1113 andlower jaw1115. The instrument is manipulated to place a biological tissue (e.g., colonic tissue) betweenjaws1113 and1115. Atissue compression device1117 is used to compress the tissue againstsurface1119 ofjaw1113.Compression device1117 is capable of ensuring a consistent, standardized pressure alongsurface1119. In one embodiment,compression device1117 is a balloon or bladder which is inflated throughtube1121 through pneumatic or hydraulic means. Thedevice1117 may incorporate one or more pressure sensors to ensure appropriate pressure is achieved. Pressure may be controlled automatically or manually with actuator orinlet1123.
• One ormore sensing elements1127 and1129 are integrated into or coupled withface1119. The sensing elements may include any of the previously described sensor types for measuring physiologic or mechanical properties. In one configuration, the sensing elements includeLEDs1127 andPDs1129, but may also take the form of other previously described emitters and detectors. In a further embodiment, the LEDs and PDs alternate across and along each of two rows. In one embodiment, the optical sensors use oximetry techniques where one or more wavelengths of light are emitted into the tissue and the absorption properties as detected by the PDs are used to assess tissue oxygenation. In one embodiment, the LEDs are configured to optically excite a phosphorescent probe injected into the tissue and the PDs detect the phosphorescent response in order to assess the oxygen-dependent quenching time.Control electronics1133 are integrated into the instrument, and may be located inupper jaw1113 oftip1103.Additional electronics1135 may be placed in the main proximal body of instrument. In one configuration,control electronics1133 injaw1113 control the sensing elements and an additional set ofcontrol electronics1135 in the main body include a power source, wired or wireless communication, and user interfaces. User interfaces may include one or more buttons, displays, or audio devices. In oneconfiguration control electronics1133 manage control of thesensing elements1127 and1129 and determine information about the oxygen-dependent quenching time that is used to assess tissue oxygenation at one or more points alongsurface1119.
• In one embodiment, needles1137 are used to locally inject a medium containing the phosphorescent oxygen sensing probe. Theneedles1137 may be small standard needles, a micromolded needle array, porous needles with individual or a plethora of holes on the side and occluded tips, or other variations for distributing the medium in the tissue. In one configurationmultiple needles1137 andmultiple sensing elements1127 and1129 provide a distribution of oxygenation in the tissue alongface1119.Needles1137 may be configured to inject varying amounts to varying depths in the tissue. In one configuration, the sensing instrument is configured to asses a distribution of tissue oxygenation along both sides of layered or tubular tissue.LEDs1127 sequentially emit two wavelengths, and the time response for each wavelength provides information about the oxygen-dependent quenching at different depths in the tissue as described previously. In one configuration, one ormore needles1137 are inserted laterally along the length of the jaws through the tissue.
• Theneedles1137 are connected throughtube1141 toreservoir1143.Tube1141 may pass through the center axis of articulated joint1107.Reservoir1143 contains medium1145. The medium may contain a phosphorescent oxygen sensing molecular probe. In one embodiment,plunger1149 is used to inject metered doses of medium1145 from sealedvessel1143. Thevessel1143 may contain a specific number or predefined does of the medium1143. In one embodiment, anactuation device1153 controls the motion ofpiston1149. Theactuation device1153 may be a screw or ratcheting device controlled by the user. It may take the form of a motorized or robotic actuation. The sensing instrument may be used with either locally injected medium, or systemically injected medium. Locally injected medium may be delivered using apparatus associated with the sensing instrument as described, or may be injected using a stand-alone device. In one configuration, a stand-alone device is placed betweenjaws1113 and1115 and the medium is ejected into the tissue.Jaws1113 and1115 may be fixed, move in a parallel motion, or close in a hinged motion.
• Thesensing instrument1101 may be used as a stand-alone device. In an alternate configuration, a wired orwireless connection1157 connects to a base station and/or one or more sensing instruments. The instrument may be a minimally invasive surgical instrument, used for traditional open surgical procedures, or configured for external use. The techniques shown inFIG. 11 are directly applicable to other surgical instruments. In one embodiment,instrument1101 is a surgical stapler anvil. The anvil has aface1119 that incorporates stapleforms Sensing elements1127 and1129 are configured such that they are at the tissue contact face ofsurface1119.Needles1137 may be integrated into theanvil surface1119, an opposing surface, or an internal cavity. In one configuration,reservoir1143 is contained inside a cavity in the anvil and aplunger1149 is actuated as the anvil compresses the tissue against an opposing surface, thus injecting the tissue with the medium1143 as the stapler is closed and the anvil is compressing the tissue against an opposing surface on the stapler body. In one configuration, the medium1143 is contained in a reservoir in a separate apparatus which is placed between the anvil and the opposing surface. In this configuration, the reservoir is compressed and ejects the medium into the tissue as the anvil compresses the tissue. This invention includes configurations of the described embodiments wherein an injection system is contained within the sensing device and also configurations wherein the injector is external to the sensing device and may be operated independently.
• FIG. 12 shows one embodiment of the invention, where sensors are coupled with a bite block or other piece ofmedical equipment1201 attaching to a patient'sface1203. In one configuration, abite block1201 is placed over the mouth ofpatient1203.Bite block1201 has aguide1205 andclamping device1207. In one configuration,bite block1201 is configured to hold an endotracheal (ET)tube1217. In one configuration,ET Tube1217 incorporates or is coupled to asensor1219 at the distal end.Sensor1219 may be a single point sensor, a multi-point sensor, or an imaging array. In another configuration, sensor orsensors1221 are placed along the surface of theET tube1217. One or more sensors may be place circumferentially and longitudinally along the tube. It should be noted thattube1217 is shown as an ET tube as an example, however the present invention includes all such tubes, catheters, cannulas, scopes, needles, and similar rigid and flexible devices for insertion into the mouth or other orifice of the body. Such sensing devices may be used with or without theblock1201. In one embodiment,sensor1225 operates inside of the patient's mouth.Sensor1225 may be in contact withtongue1227. Thesensor1225 may rest on the surface of thetongue1227, clip to the tongue, or sense sublingually. Thesensor1225 may also be used to sense at other locations in or around the mouth including tongue, cheek, lips, or gums. In a further embodiment, asensor1231 is coupled to theblock1201 and in direct contact with thepatient1203. Thesensor1231 may be in contact with the patient's face, cheek, lip, gum, or another external tissue.
• It should be noted that these sensing techniques can be applied to othermedical devices1201 attached topatient1203. In one exemplary configuration, amask1201 is placed over the patient. One ormore sensors1231 may be coupled with the mask itself, or one ormore sensors1215 may be coupled with thestrap1213. Similarly,sensors1215 may reside in a band or strap that may or may not contain another medical device, and may be affixed to the head or other part of the body. In one configuration, a sensor may be used to determine tissue oxygenation or other properties at the skin surface, or at a depth below the surface. Such sensing may include assessing tissue oxygenation or other properties of tissue within the skull. A further exemplary configuration is a wearable strap orband1213 with one ormore sensors1215 coupled with it. In one embodiment, one or more ofsensors1215,1219,1221, and1231 are configured to sense oxygenation through the use of phosphorescent oxygen sensing probes as described herein. In a further configuration, injection devices to introduce a medium containing the probe may be coupled with the sensors. In an alternate configuration, the probe is injected locally by other means or systemically. The described techniques for manipulating the position or flow of the probe may be applied with this device, as with other devices presented in the invention. Thesensors1215,1219,1221, and1231 may be configured to sense oxygenation by oxygen-dependent quenching of a phosphorescent probe, tissue spectroscopy, pulse oximetry, or other means, and/or may be configured to sense blood flow or perfusion, and/or may be configured to sense mechanical properties of the tissue or tissue sensor-interaction. In one embodiment the device takes the form of a bite block or indwelling line/tube such as an intracranial, endotracheal, oro/naso gastric tube, venous catheter, arterial catheter, chest tube, feeding tube, urinary catheter, or rectal tube. The sensor elements are positioned on said tube in a manner such that they contact biological tissue. Phosphorescent medium is either systemically or locally injected into subject tissue. Previously described techniques for injection as well as maintaining the location of the medium in the vicinity of the sensor may be utilized with the configurations described herein.
• FIG. 13ashows an embodiment of the present invention configured for ophthalmologic applications, such as retinal surgery. The figure showseye1301 ofpatient1303 withcornea1309,lens1311, andiris1313. In one embodiment, a surgical instrument orimaging device1331 is used to assess oxygenation or perfusion inretina1315 or atoptic nerve1319. In one configuration, instrument enters the vitreous1323 of the eye through thesclera1327. The present invention describes a sensing instrument that can be inserted into the eye through thesclera1327, or through another entry point and is capable of sensing oxygenation, perfusion, or other physiologic or mechanical properties within theeye1301. In one configuration,instrument1331 includes a sensor orsensing element1335 at the distal end of the instrument. Thesensor1335 may come in direct contact with the retina1315 (or other feature), or may be used at a distance from the surface.Sensor1335 may be a single point sensor, an imaging system such as a camera system, or a fiberoptic or other light transmission bundle connecting to an external imaging system. The previously described mapping and imaging techniques may be used. In one configuration a micro camera system is utilized at thetip1335 of the instrument. In another configuration, light guides such as an optical fiber bundle or other means of light transmission such as GRIN lenses are utilized tocouple tip1335 with a remote detector distal toinstrument1331 such as a camera.Sensor1335 may be used to assess tissue oxygenation at a single location, swept to generate a map of tissue oxygenation, or may directly generate a2D map.
• In one configuration, an injector orinjection system1339 is used to inject a medium containing an oxygen sensing molecular probe into theretina1315 or other tissue. In other configurations, an external injector provides a local injection of the probe or a systemic injection is performed to infuse the tissue with the oxygen sensing probe. Theinstrument1331 may be manipulated manually, or it may be moved using a robots or other externally controlled means. In one embodiment,instrument1331 is manipulated to scansensor1335 across the tissue surface to determine the oxygenation and/or other properties at multiple points. In a further configuration, by manipulating the tool, a2D or3D oxygenation map of theretina1315 is generated. The map may be fused with camera-based imaging to generate an overlay of oxygenation or flow on a video steam or captured still image. In one configuration, traditional endoscopy images are collected along with quantitative oxygen maps and are visualized together. In another embodiment, the imaging device does not enter the vitreous1323, and rather images the oxygenation in the eye throughlens1311. The sensing instrument may be a stand-alone device, used in conjunction with imaging or surgical equipment such as an endoscope, or integrated into or coupled with such imaging or surgical equipment. In a further embodiment,sensor1335 ofinstrument1331 is configured to image beyond the retina to a point within the skull, including the brain. The describedsensor1335 may be configured to assess tissue oxygenation based on oxygen-dependent quenching of a molecular probe as described herein. It may further incorporate other optical or mechanical sensing techniques as described elsewhere in this disclosure.
• FIG. 13bshows an embodiment of the invention where thesensing device1351, or component thereof, is external to theeye1301. Thesensing device1351 rests on or is external to thecornea1309 for non-invasive sensing of oxygenation in the eye. In one configuration,sensing device1351 takes the form of a contact lens or other similar object substantially made of hydrogel or similar material and rests oncornea1309. In another configuration,sensing device1351 is a patch that rests over theeye1301 and may rest onface1303. In an alternate configuration, thesensing device1351 is externally mounted. One example of an externally mounteddevice1351 is coupled to or incorporated into a surgical microscope. In another embodiment, thesensing device1351 is mounted to or incorporated into a pair of eye glasses, goggles, or eyewear. In another embodiment,sensor1351 is configured to act as a biometricdevice interrogating retina1315, or theoptic nerve1319 to aid in identification of an individual.
• Sensing device contains one ormore sensing elements1355. The sensing elements may be integral parts ofsensing device1351, or connected via optical fibers, wires, wireless, or other means. In one embodiment, the sensing device is configured to assess oxygenation through optical means of monitoring an injected molecular phosphorescent probe that has oxygen-dependent phosphorescent quenching characteristics. In one configuration thesensing elements1355 emit one or more wavelengths of light1357 through the eye'slens1311 such that it focusses on apoint1359, such as the fovea, on theoptic nerve1319. One application of the invention is to assess oxygenation of the optic nerve, as a proxy for brain oxygenation. In another application, the invention is used to determine a map of oxygenation at and near theoptic nerve1319 and/or on theretina1315. The exemplary embodiment of an oxygen imaging system (i.e. oxygen mapping system) shown inFIG. 3 is only an example configuration and this approach may be utilized in other configurations for other organ systems. In a further embodiment, this approach is utilized for assessing oxygenation of gastrointestinal tissue. It may be further utilized in the previously described approach to measure oxygenation before, during, and after CRC surgery.
• Accordingly, one embodiment includes a surgical device for sensing tissue oxygenation based upon oxygen-dependent quenching of phosphorescent oxygen sensing probe.
• Another embodiment includes a minimally invasive surgical device configured to sense oxygenation of a tissue, wherein the sensing is based upon oxygen-dependent quenching of a phosphorescent oxygen sensing probe, wherein the surgical device contains a mechanism for ensuring consistent compression pressure the tissue.
• Another embodiment includes a surgical instrument for manipulating biological tissue, wherein the instrument incorporates an injection system configured to deliver a medium consisting in part of oxygen sensing probes to the tissue, wherein the instrument further incorporates a sensing system configured to detect the optical response the oxygen sensing probes in the tissue.
• Another embodiment includes a surgical instrument for manipulating biological tissue, wherein the instrument incorporates a microneedle array configured to inject a medium into the tissue.
• Another embodiment includes a surgical instrument for manipulation biological tissue, wherein the instrument incorporates a microinjection system into the body of the instrument, wherein the instrument further comprises one or more needles, wherein the microinjection system controls injection of a medium into the tissue through the one or more needles.
• Another embodiment includes a surgical instrument for manipulating biological tissue, wherein the instrument comprises a means for producing constant compression pressure of the tissue against a surface of the instrument.
• Another embodiment includes a method for manipulating an injectable probe, wherein the probe incorporates ferromagnetic material, and wherein a surgical instrument incorporates magnets to attract and retain the molecule.
• Another embodiment includes a surgical instrument incorporating a means for injection of medium into tissue, and further incorporates a means for manipulating the location of the medium in the tissue after injection.
• Another embodiment includes a method for maintaining the position of a locally injected probe at the injection site in a tissue for a period of time, wherein bioabsorbable entities are incorporated into the probes structure, wherein the bioabsorbable entities detach from the probe and enable clearance from the body.
• Another embodiment includes a surgical instrument comprising a high speed imaging system, wherein the imaging system is configured to provide data to generate maps of tissue oxygenation based upon phosphorescent optical response of an injectable oxygen sensing probe.
• Another embodiment includes a surgical instrument for eye surgery comprising an imaging system, wherein the imaging system is configured to provide data to generate maps of tissue oxygenation based upon phosphorescent quenching time of an oxygen sensing probe.
• Another embodiment includes a method of sensing and discriminating oxygenation in two or more layers of tissue, wherein oxygenation is sensed based on phosphorescent quenching time of a phosphorescent oxygen sensing probe, wherein the probe has multiple absorption wavelengths that have different tissue absorption properties, wherein the oxygen is discriminated based on the phosphorescent response due to multiple emission wavelengths.
• Another embodiment includes a surgical system comprising a surgical instrument configured to assess tissue oxygenation, and further comprising a sensing device configured to assess tissue oxygenation that couples to a surgical stapler, wherein both devices are configured to assess oxygenation of tissue using an injectable phosphorescent oxygen sensing probe, wherein the system is configured to provide information to guide a surgical procedure.
• Another embodiment includes a surgical instrument comprising a plurality of sensing elements, wherein at least the instrument is configured to assess tissue oxygenation based on the quenching time of an injectable phosphorescent probe, wherein the instrument is further configured to sense at least one of tissue oxygenation, blood oxygenation, pulse rate, pulse presence, pulse rhythm, tissue perfusion, staple gap, compression force, tissue interaction force, fluorescence, tissue electrical impedance, tissue electrical activity, pH, concentration of cellular respiration metabolites, electromyography, temperature, fluid flow rate, fluid flow volume, tissue pressure, blood pressure, biomarkers, radiotracers, immunologic characteristics, biochemical characteristics, nerve activity, an evoked potential, oxygen delivery, oxygen utilization, tissue characterization, tissue general health, tissue flow dynamics, tissue chemical composition, tissue immunologic activity, tissue pathogen concentration, tissue water content, blood hemoglobin content, tissue chromophore content, tissue neoplastic cell content and tissue dysplastic cell content.
• Another embodiment includes a surgical instrument configured to assess tissue oxygenation based on the quenching time of an injectable phosphorescent probe, wherein the instrument is communicatively coupled via a wireless connection to a base station.
• Another embodiment includes a sensing surgical stapler anvil, wherein the sensing anvil is configured to assess oxygenation of tissue at the site of an anastomosis, wherein the assessment of oxygenation is based on the quenching time of an injectable phosphorescent probe.
• Another embodiment includes an adjunct to a surgical stapler incorporating at least one sensor configured to assess the oxygenation of a tissue based on the quenching time of an injectable phosphorescent probe, wherein the adjunct operates independently of the stapler; wherein adjunct is optionally coupled to the stapler.
• Another embodiment includes a medical device configured for sensing tissue oxygenation, the device comprising at least one sensor, wherein at least one sensor of the device detects the optical response of an oxygen sensing probe.
• The oxygen sensing probe has a phosphorescent optical response, wherein the phosphorescent lifetime of the optical response is responsive to oxygen-dependent quenching.
• The medical device may be a surgical instrument for manipulating biological tissue.
• The medical device may be a minimally invasive surgical instrument.
• The surgical instrument further comprises a mechanism for ensuring consistent compression pressure on the tissue.
• The surgical instrument may further comprises an injection system configured to deliver a medium to the tissue, wherein the medium includes the oxygen sensing probes.
• The injection system may comprise a plethora of microneedles.
• The surgical instrument may be a surgical stapler or component thereof.
• The surgical instrument may be a surgical stapler anvil with integrated oxygen sensing capabilities.
• The medical device may be patch with integrated oxygen sensing capabilities.
• The patch may be fully or partially bioabsorbable.
• The medical device may be communicatively coupled via a wireless connection to a base station.
• A surgical stapler anvil may be configured to assess oxygenation of a biological tissue at the site of an anastomosis, wherein the assessment of oxygenation is based on oxygen-dependent quenching time of an injectable phosphorescent probe.
• An adjunct to a surgical stapler incorporating at least one sensor may be configured to assess oxygenation of a biological tissue, wherein the assessment of oxygenation is based on oxygen-dependent quenching time of an injectable phosphorescent probe, wherein the adjunct operates independently of the stapler; wherein adjunct is optionally coupled to the stapler.
• A surgical system may include a surgical instrument configured to assess oxygenation of a biological tissue, and a sensing device configured to assess tissue oxygenation that couples to a surgical stapler. The devices are configured to assess oxygenation of biological tissue using an injectable phosphorescent oxygen sensing probe.
• The surgical system may be further configured to provide information to guide a surgical procedure.
• An imaging system may be configured to provide a2D map of tissue oxygenation, wherein the imaging system generates the map responsive to oxygen-dependent quenching of the phosphorescent response of oxygen sensitive molecules.
• The imaging system may be incorporated into a surgical instrument.
• The surgical instrument may be configured for ophthalmic surgery, wherein the instrument provides an oxygenation map of the retina.
• The surgical instrument may be configured to provide an oxygen map of gastrointestinal tissue.
• The surgical instrument may be configured to assist in analysis of a target tissue, wherein analysis is performed for at least one of before, during, and after creation of an anastomosis.
• A surgical instrument may manipulate ipulating biological tissue, wherein the instrument incorporates a microinjection system into the body of the instrument, wherein the instrument further comprises one or more needles, wherein the microinjection system controls injection of a medium into the tissue through the one or more needles.
• A surgical instrument may manipulate biological tissue, wherein the instrument comprises a means for producing constant compression pressure of the tissue against a surface of the instrument.
• A method may manipulate an injectable probe, wherein the probe incorporates ferromagnetic material, and wherein a surgical instrument incorporates magnets to attract and retain the molecule.
• A surgical instrument may incorporate a means for injection of medium into tissue, and further incorporates a means for manipulating the location of the medium in the tissue after injection.
• A method may maintain the position of a locally injected probe at the injection site in a tissue for a period of time, wherein bioabsorbable entities are incorporated into the probes structure, wherein the bioabsorbable entities detach from the probe and enable clearance from the body.
• A method of sensing and discriminating oxygenation in two or more layers of tissue may be provided, wherein oxygenation is sensed based on phosphorescent quenching time of a phosphorescent oxygen sensing probe, wherein the probe has multiple absorption wavelengths that have different tissue absorption properties, wherein the oxygen is discriminated based on the phosphorescent response due to multiple emission wavelengths.
• A surgical instrument may comprise a plurality of sensing elements, wherein at least the instrument is configured to assess tissue oxygenation based on the quenching time of an injectable phosphorescent probe, wherein the instrument is further configured to sense at least one of tissue oxygenation, blood oxygenation, pulse rate, pulse presence, pulse rhythm, tissue perfusion, staple gap, compression force, tissue interaction force, fluorescence, tissue electrical impedance, tissue electrical activity, pH, concentration of cellular respiration metabolites, electromyography, temperature, fluid flow rate, fluid flow volume, tissue pressure, blood pressure, biomarkers, radiotracers, immunologic characteristics, biochemical characteristics, nerve activity, an evoked potential, oxygen delivery, oxygen utilization, tissue characterization, tissue general health, tissue flow dynamics, tissue chemical composition, tissue immunologic activity, tissue pathogen concentration, tissue water content, blood hemoglobin content, tissue chromophore content, tissue neoplastic cell content and tissue dysplastic cell content.
• The embodiments described above demonstrate how oxygen sensitive probes can be utilized with surgical instruments incorporating sensors. These embodiments are meant as illustrative purposes. The described sensing instruments and approaches can be adapted to provide the described functionalities for other surgical instruments or medical devices. Further, the techniques discussed should not be construed to be limited to use only with phosphorescent oxygen sensing probes. Further still, all references to phosphorescence or fluorescence more broadly may be applied to all light-remitting phenomenon.
• The present invention can be practiced by employing conventional materials, methodology and equipment. Accordingly, the details of such materials, equipment and methodology are not set forth herein in detail. In the previous descriptions, numerous specific details are set forth, such as specific materials, structures, chemicals, processes, etc., in order to provide a thorough understanding of the present invention. However, it should be recognized that the present invention can be practiced without resorting to the details specifically set forth. In other instances, well known processing structures have not been described in detail, in order not to unnecessarily obscure the present invention.
• Only exemplary embodiments of the present invention and but a few examples of its versatility are shown and described in the present disclosure. It is to be understood that the present invention is capable of use in various other combinations and environments and is capable of changes or modifications within the scope of the inventive concept as expressed herein.
• Although the foregoing description is directed to the preferred embodiments of the invention, it is noted that other variations and modifications will be apparent to those skilled in the art, and may be made without departing from the spirit or scope of the invention. Moreover, features described in connection with one embodiment of the invention may be used in conjunction with other embodiments, even if not explicitly stated above.

Claims (2)

1. A surgical instrument configured to sense a light re-emitting probe to resolve tissue oxygenation, the surgical instrument comprising:

at least one optical emitter that is configured to excite the light re-emitting probe within an absorption band of the light re-emitting probe;

at least one optical detector configured to receive the re-emitted light from the probe; and

a signal processor that is configured to resolve the tissue oxygenation based on the received light.

2.-30. (canceled)

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