US20080032408A1 - Tag Nucleic Acids and Probe Arrays - Google Patents

Abstract

The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses.

Description

PRIORITY CLAIM
• This application claims priority of U.S. Provisional Application 60/195,585 filed Apr. 6, 2000 entitled “Tag Nucleic Acids and Probe Arrays”, which is incorporated herein by reference for all purposes in its entirety.
FIELD OF THE INVENTION
• This invention provides sets of nucleic acid tags, arrays of oligonucleotide probes, nucleic acid-tagged sets of recombinant cells and other compositions. The invention relates to the selection and interaction of nucleic acids, and nucleic acids immobilized to solid substrates, including related chemistry, biology, and medical diagnostic uses.
BACKGROUND OF THE INVENTION
• The use of short nucleic acid sequences as “tags” to identify specific biological substances in a sample is known. For example, tags may be used as a method of or as labels for a wide variety of biological and nonbiological materials, see, for example, Dollinger, The Polymerase Chain Reaction pp. 265-274 Mullis et al., editors (Birkhauser, Boston, 1994) or as a method of screening complex chemical libraries. See, for example, Alper, Science, 264: 1399-1401 (1994); and Needels et al. PNAS 90, 10700-10704 (1993). See also U.S. Pat. Nos. 4,359,353, 4,441,943, 5,451,505 and 5,654,413.
• There is great necessity for sets of tag sequences which are known to hybridize effectively to their complementary probe sequences with minimal cross-hybridization between the different tag sequences. The presently claimed invention provides sets of tag sequences, tag sequence kits, and methods of using tag sequences which fulfill these requirements.
SUMMARY OF THE INVENTION
• The presently claimed invention provides 2050 unique sequences which have been specifically chosen according to strict criteria to produce sequences suitable for a wide variety of “tagging” applications. These sequences are provided as SEQ ID NOs 1-2050.
• In one embodiment, some or all of SEQ ID Nos 1-2050 comprise tag sequences. In a further embodiment, some or all of SEQ ID Nos 1-2050 comprise tag-probe sequences. In a further embodiment, the tag-probe sequences are immobilized to a solid support.
• The unique sequences of the presently claimed invention may be used alone or in combinations of 10 or more, 100 or more, 200 or more, 500 or more, 1000 or more, 1500 or more, or 2000 or more as nucleic acid tags and/or tag-probes.
BRIEF DESCRIPTION OF THE DRAWINGS
• FIG. 1 shows a plot of the discrimination score and the signal intensity for 2200 candidate sequences.
• FIG. 2 shows an example of the sequences attached to each of the four array features representing a given tag sequence. Four features, organized vertically on the probe array, represent each tag-probe.
• FIG. 3 shows the array features from an array designed to probe for the tag sequences of the presently claimed invention. For each of the four tag-probes shown, arranged horizontally across the array, the brightest hybridization signal is seen with the “PM” feature.
• FIG. 4 is a scanned image of the hybridization patterns resulting from the hybridization of2050 different probes containing regions complementary to the SEQ ID Nos 1-2050 to an array comprised of tag-probes corresponding to SEQ ID Nos 1-2050.
• FIG. 5 is a scanned image of the hybridization patterns resulting from the hybridization of 50 different probes containing regions complementary to SEQ ID Nos 2001-2050 to an array identical to the array depicted inFIG. 4.
• FIG. 6 shows signal intensities from two different independent experiments in which 2000 biotinylated oligonucleotide tags or 50 fluorescein labeled control oligonucleotides were hybridized to arrays designed as described above.
• FIG. 7 shows the PM/MM ratios from the data described inFIG. 4 above.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTSI. Definitions
• As used herein, certain terms may have the following defined meanings.
• As used in the specification and claims, the singular forms “a”, “an” and “the” include plural references unless the context clearly dictates otherwise. For example, the term “an array” may include a plurality of arrays unless the context clearly dictates otherwise.
• An “array” represents an intentionally created collection of molecules which can be prepared either synthetically or biosynthetically. In particular, the term “array” herein means an intentionally created collection of polynucleotides attached to at least a first surface of at least one solid support wherein the identity of each polynucleotide at a given predefined region is known. The terms “array,” “biological chip” and “chip” are used interchangeably.
• The array of molecules can be screened for biological activity in a variety of different formats (e.g., libraries of soluble molecules, libraries of compounds tethered to resin beads, fibers, silica chips, or other solid supports). The fabrication of polynucleotide arrays on a solid substrate, and methods of use of the arrays in different assays, are described in U.S. Pat. Nos.: 5,143,854, 5,242,979, 5,252,743, 5,324,663, 5,384,261, 5,405,783, 5,412,087, 5,424,186, 5,445,934, 5,451,683, 5,482,867, 5,489,678, 5,491,074, 5,510,270, 5,527,681, 5,550,215, 5,571,639, 5,593,839, 5,599,695, 5,624,711, 5,631,734, 5,677,195, 5,744,101, 5,744,305, 5,744,992, 5,753,788, 5,770,456, 5,831,070, 5,856,011, 6,040,138 and 6,040,193 all of which are incorporated by reference herein in their entireties for all purposes. See also, U.S. Ser. Nos. 09/079,324, 09/122,216, and PCT Application WO US99/00730 each of which is incorporated by reference herein in its entirety for all purposes. Preferred arrays contemplated by the presently claimed invention have the probe densities as described in the above referenced patents. For example, the '305 patent discloses 100, 400, 1,000 and 10,000 probes/cm². “Solid support,” “support,” and “substrate” refer to a material or group of materials having a rigid or semi-rigid surface or surfaces. In many embodiments, at least one surface of the solid support will be substantially flat, although in some embodiments it may be desirable to physically separate synthesis regions for different compounds with, for example, wells, raised regions, pins, etched trenches, or the like. According to other embodiments, the solid support(s) will take the form of beads, resins, gels, microspheres, fibers or other geometric configurations.
• A “discrete, known location” refers to a localized area on a solid support which is, was, or is intended to be used for placement or fabrication of a selected molecule and is otherwise referred to herein in the alternative as a “selected” region. The discrete, known location may have any convenient shape, e.g., circular, rectangular, elliptical, wedge-shaped, etc. For the sake of brevity herein, “discrete, known locations” are sometimes referred to as “predefined regions,” “regions,” or “features.” In some embodiments, a discrete, known location and, therefore, the area upon which each distinct compound is synthesized is smaller than about 1 cm or even less than 1 mm². In additional embodiments, a discrete, known location can be achieved by physically separating the regions (i.e., beads, fibers, resins, gels, etc.) into wells, trays, etc.
• As used herein, a “polynucleotide” is a sequence of two or more nucleotides. Polynucleotides of the present invention include sequences of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) which may be isolated from natural sources, recombinantly produced, or artificially synthesized. A further example of a polynucleotide of the present invention may be polyamide polynucleotide or peptide nucleic acid (PNA). This invention also encompasses situations in which there is nontraditional base pairing such as Hoogsteen base pairing which has been identified in certain tRNA molecules and postulated to exist in a triple helix. “Polynucleotide” is used interchangeably with “oligonucleotide” is this application.
• The terms “nucleotide” and “nucleic acid base” include deoxynucleotides and analogs thereof. These analogs are those molecules having some structural features in common with a naturally occurring nucleotide such that when incorporated into a polynucleotide sequence, they allow hybridization with a complementary polynucleotide in solution. Typically, these analogs may have one or more modified bases, as well as modified forms of ribose and phosphodiester moieties. The changes can be tailor made to stabilize or destabilize hybrid formation, enhance the specificity of hybridization with a complementary polynucleotide sequence as desired, or enhance stability of the polynucleotide.
• The terms “nucleic acid,” “nucleic acid molecule,” or “nucleic acid sequence,” refer to a deoxyribonucleotide or ribonucleotide polymer in either single-or double-stranded form, and unless otherwise limited, would encompass analogs of natural nucleotides that can function in a similar manner as naturally occurring nucleotides. Nucleic acids may be derived from a variety or sources including, but not limited to, naturally occurring nucleic acids, clones, synthesis in solution or solid phase synthesis.
• As used herein a “probe” is defined as a nucleic acid capable of binding to a target nucleic acid of complementary sequence through one or more types of chemical bonds, usually through complementary base pairing, usually through hydrogen bond formation. As used herein, a probe may include natural (i.e. A, G, U, C, or T) or modified bases (7-deazaguanosine, inosine, etc.). In addition, the bases in probes may be joined by a linkage other than a phosphodiester bond, so long as it does not interfere with hybridization. Thus, probes may be peptide nucleic acids in which the constituent bases are joined by peptide bonds rather than phosphodiester linkages.
• The term “target nucleic acid” or “target sequence” refers to a nucleic acid or nucleic acid sequence which is to be analyzed. A target can be a nucleic acid to which a probe will hybridize. The probe may or may not be specifically designed to hybridize to the target. It is either the presence or absence of the target nucleic acid that is to be detected, or the amount of the target nucleic acid that is to be quantified. The term target nucleic acid may refer to the specific subsequence of a larger nucleic acid to which the probe is directed or to the overall sequence (e.g., gene or mRNA) whose expression level it is desired to detect. The difference in usage will be apparent from context.
• The term “hybridization” refers to the process in which two single-stranded polynucleotides bind non-covalently to form a stable double-stranded polynucleotide; triple-stranded hybridization is also theoretically possible. The resulting (usually) double-stranded polynucleotide is a “hybrid.” The proportion of the population of polynucleotides that forms stable hybrids is referred to herein as the “degree of hybridization.” Hybrids can contain two DNA strands, two RNA strands, or one DNA and one RNA strand.
• Methods for conducting polynucleotide hybridization assays have been well developed in the art. Hybridization assay procedures and conditions will vary depending on the application and are selected in accordance with the general binding methods known including those referred to in: Molecular Cloning, A Laboratory Manual, Second Ed., J. Sambrook et al., Eds., Cold Spring Harbor Laboratory Press, 1989 (“Sambrook et al.”); Berger and Kimmel, “Methods in Enzymology,” Vol. 152, “Guide to Molecular Cloning Techniques”, Academic Press, Inc., San Diego, Calif., 1987; Young and Davis,Proc. Natl. Acad. Sci.,U.S.A., 80:1194 (1983), each of which are incorporated herein by reference.
• It is appreciated that the ability of two single stranded polynucleotides to hybridize will depend upon factors such as their degree of complementarity as well as the stringency of the hybridization reaction conditions.
• As used herein, “stringency” refers to the conditions of a hybridization reaction that influence the degree to which polynucleotides hybridize. Stringent conditions can be selected that allow polynucleotide duplexes to be distinguished based on their degree of mismatch. High stringency is correlated with a lower probability for the formation of a duplex containing mismatched bases. Thus, the higher the stringency, the greater the probability that two single-stranded polynucleotides, capable of forming a mismatched duplex, will remain single-stranded. Conversely, at lower stringency, the probability of formation of a mismatched duplex is increased.
• A nucleic acid “tag” is a selected nucleic acid with a specified nucleic acid sequence. A nucleic acid “probe” hybridizes to a nucleic acid “tag.”
• A nucleic acid “tag-probe” is a specific sequence capable of hybridizing to a specific “tag.” Typically, the “tag-probe” is the complement or a partial complement of the “tag.” In one typical configuration, nucleic acid tags are incorporated as labels into biological libraries, and the tag nucleic acids are detected using a microarray.
• Throughout this disclosure, various aspects of this invention are presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention.
• Accordingly, the description of a range should be considered to have specifically disclosed all the possible sub-ranges as well as individual numerical values within that range. For example, the description of a range such as 4 to 50 should be considered to have specifically disclosed all integers within the sub-ranges such as 4 to 10, 4 to 20, 4 to 30, 4 to 40, 4 to 50, 5 to 10, 5 to 20 etc., as well as individual numbers within that range, for example, 6, 8, 15, 20, 32, 39, 43, 48 etc. This applies regardless of the breadth of the range. Likewise, a description of a range such as 1 or more, 10 or more, 10³or more, 10⁶or more, or 10¹²or more should be considered to have specifically disclosed individual numbers within that range as well as higher numbers, for example, 20, 2×10⁴, 3×10⁸, 4×10¹⁵, 5×10¹⁸, etc.
• Various patents, patent applications and publications are referenced throughout the specification, unless otherwise indicated, each is incorporated by reference in its entirety for all purposes.
II. General
• The presently claimed invention provides 2050 unique sequences which have been specifically chosen according to strict criteria to produce sequences suitable for a wide variety of “tagging” applications. These sequences are provided as SEQ ID NOs 1-2050.
• In one embodiment, some or all of SEQ ID Nos 1-2050 comprise tag sequences. In a further embodiment, some or all of SEQ ID Nos 1-2050 comprise tag-probe sequences. In a further embodiment, the tag-probe sequences are immobilized to a solid support.
• An initial set of 2200 20 mer sequences was selected with closely matched melting temperatures. A further filter based on rules such as those described in U.S. Provisional Patent Application 60/176,520 was applied to optimized and standardize the hybridization characteristics of the set. Finally, sequences were removed if they were identical or nearly identical to each other or to sequences in the public databases. This reduced the pool of candidate sequences to 2200. The hybridization performance of the entire set of 2200 candidate sequences was evaluated. Labeled oligonucleotides complementary to the candidate sequences were synthesized and hybridized to an array containing probes designed to analyze the performance of all 2200 candidate sequences. The array contained four different sequences to interrogate each candidate sequence. A probe designed to be the perfect match complement to the candidate sequence (PM), a probe designed to have a central mismatch at position 10 (MM), and probes designed to be the complements to the PM and MM probes (cPM and cMM respectively).
• FIG. 1 shows a plot of the discrimination score and the signal intensity for all 2200 sequences. A line was fitted to select the 2050 sequences with the highest discrimination and signal intensity. These 2050 sequences are SEQ ID Nos. 1-2050.
• In one embodiment of the invention, the sequences of the presently claimed invention are tag-probes attached to a solid support. Methods of immobilizing presynthesized sequences and synthesizing sequences de novo on solid supports are known. See for example, U.S. Pat. Nos.: 5,143,854, 5,242,979, 5,252,743, 5,324,663, 5,384,261, 5,405,783, 5,412,087, 5,424,186, 5,445,934, 5,451,683, 5,482,867, 5,489,678, 5,491,074, 5,510,270, 5,527,681, 5,550,215, 5,571,639, 5,593,839, 5,599,695, 5,624,711, 5,631,734, 5,677,195, 5,744,101, 5,744,305, 5,753,788, 5,770,456, 5,831,070, 5,856,011, 5,744,992, 6,040,138, 6,040,193, U.S. Ser. Nos. 09/079,324, 09/122,216, and PCT Application WO US99/00730.
• In this and other embodiments it is often useful to provide control probes. As one example, SEQ ID Nos. 1-2000 may comprise the tag-probes and SEQ ID Nos. 2001-2050 may comprise the control probes. In a preferred embodiment, the control probes are representative of the population with respect to observed signal intensities and discrimination. In a further preferred embodiment, tag sequences with relatively low signals may be over-represented in the control sequences so as to increase information about the sensitivity of experiments at the lower limit of detection.
METHODS OF USE
• The use of short nucleic acid sequences as “tags” to identify specific biological substances in a sample is known. For example, tags may be used as a method of or as labels for a wide variety of biological and nonbiological materials, see, for example, Dollinger, The Polymerase Chain Reaction pp. 265-274 Mullis et al., editors (Birkhauser, Boston, 1994) or as a method of screening complex chemical libraries. See, for example, Brenner and Lerner, PNAS 89, 5281-5383 (1992); Alper, Science, 264: 1399-1401 (1994); and Needels et al. PNAS 90, 10700-10704 (1993). See also U.S. Pat. Nos. 4,359,353, 4,441,943, 5,451,505, 5,149,625, 5,654,413 and 5,800,992.
• In addition to those applications above, the presently claimed sequences are suitable to be employed for any of the methods described in U.S. patent application Ser. No. 08/626,285 (filed Apr. 4, 1996), including as a method of analysis of genomic DNA. For example, as described in the '285 application, tag arrays may be used to identify the function of identified open reading frames (ORFs) by creating deletion mutants for each ORF and analyzing the resulting deletion mutants under a wide variety of selective conditions.
• U.S. Provisional Patent Application No. 60/140,359 (filed Jun. 23, 1999) described methods of using tag arrays and the single base extension reaction for genotyping and other types of biological analysis. A set of tags and a tag array derived from Seq. ID Nos. 1-2000 and their complements are suitable to be used for the methods described in this application. Briefly, the '359 application describes methods of determining the genotype of an individual at a polymorphic locus or the frequency of alleles in a population. One embodiment of the method involves three step: (1) amplification of the polymorphic locus, (2) primer extension of a sequence-tagged primer with distinct labels for different polynucleotides at the polymorphic locus, and (3) hybridization to a tag array. The amount of each distinct label can be determined at known positions of the tag array. Each tag represents a distinct polymorphic locus and each distinct label represents a distinct allelic form at the polymorphic locus. The method permits the simultaneous determination of a genotype at multiple loci, as well as the determination of allele frequencies in a population. Another embodiment employs just steps (2) and (3).
• Table 1, below, lists the sequences of the presently claimed invention.Column 1 lists the sequence ID number corresponding to each sequence.Column 2 lists the sequences in the 3′ to 5′ direction.

• TABLE I
  Seq. Id	3′ to 5′ sequence

  1	TAAACTAGCATTGAGCCCAC
  2	AAATCAGCAAACGGGCTCCG
  3	GAATTGATAATCGCAGCCAC
  4	GATATAGGAATGGCGCATAC
  5	CTCATCGGAAGGGCTCGTAA
  6	ACAGATGGAAAGGCAGTTCT
  7	TTTGGTAGCTGAGTGCCCTA
  8	TAACTGGTTTGACGCCACGC
  9	TAATTGAGCTGACGGCGCAC
  10	TTGTTGCTACTCTGGCCCGA
  11	TTCCGTGCATAGTATAGGGA
  12	TTATGCGACTTATCTCGGGA
  13	TGTATAGGATTATGTCCGCG
  14	CTGCTAGGAATATGAGCTAC
  15	CTTCTGTCAATATGGGTACG
  16	TATTTCGAGATATGAGGCGC
  17	TTGATCGTAGATTCGTGAGC
  18	CGAGATTACAATTCACGAGC
  19	TGGTGTCTAGCTTCCAGCCT
  20	TGAGGTCACGGTTCATGCTA
  21	TGGTTACTGGTATATGCCGC
  22	CCGAGTGCAGAATAAACCCG
  23	GCGGTCTCAATACAAACTCA
  24	GAAGCTACCATACGCGAGCA
  25	ACGGGATAACAACGCAGCCT
  26	AGAAGATCAACAGCTCGTCC
  27	ATAAGATCAAGACCTGTGCC
  28	ATTAGATTAAGACCAGCGCC
  29	ATATAATCAAGACTGGCGCG
  30	AGCATATAACCACTGATCCG
  31	ACACTATTAAAGCTGCTCCG
  32	CAATGTATAAGACTCTCGCC
  33	CACTAATTCAGACGAAGCCG
  34	GACCCTATCAGACAGATGCA
  35	CACGCATCAAGACAGTATCG
  36	CAGCTCCTAAGACTTGGACA
  37	GGTATCATAGGACATTCGCA
  38	GGTTACATGGATATAGCACC
  39	TGTGTTTCAGCTATGCAGGC
  40	TAATTCGCTGCAACCAGATC
  41	ATAATTCCAACATGGGAGCC
  42	CATTGCTTAATATGGGAGCC
  43	CAATGCTTAATACCGACACG
  44	GATTGCTTAGACCCTGCACG
  45	GATTCATTAGACCAGGCGCT
  46	GATTCTACATGCCACTAGCA
  47	CCTGCGAACTGGCCTGAATA
  48	CGCAGCGGAAGGCTCAATAA
  49	CCTACCGCAAGGCAGGATAA
  50	CCTATGATAAGGCACGCACA
  51	CGCTGTGCAAGGCTCGTATA
  52	CGATTGTCAAGGCAGTGATA
  53	CATTGCGAACTGCATCTAAC
  54	GATAGTCCAATGCTACTGAC
  55	GATTCGGTAATGCGCTGTAA
  56	GACGTTTCAATGCAGCGTAA
  57	GAGAGTGCAATGCCGACTAA
  58	GAGATCCGAATGCGCGTACT
  59	CGAGATCCAAGGCCCATGAT
  60	AGCTTGCACAGTAACCATGA
  61	AGAGTTGAACAGCATACCCT
  62	TATCTGATCGGACGGCCAGT
  63	TATTGACTACTGCGCCTCAG
  64	TTGGACTATTGGGTATCGCC
  65	TTGTCAGATTGGATGCGCTC
  66	TATGCAGAATGGCGTGTATC
  67	CATTGGATAAGCACTGATCG
  68	CCCGGAATAAGGCCACGATA
  69	CTCATAGAATGGACCAGATC
  70	CATAGATTAAGCACTCAGCC
  71	CATGATGTAAGCACGCTACC
  72	CAGGAGCGAAGCAGATACTC
  73	CAGAGCAGAAGCACTCACGT
  74	TACATAGGCTTCAGCATCAC
  75	TATTATACCTTGATCCGCGC
  76	TAAACTGCTTGCATACGGCG
  77	TATAAGCCTTGCAGCGGACC
  78	TTTAAGCGGTGGATCTAGCT
  79	TTAATAGCCTTGAGCAGCGA
  80	ATAAATGCTTGGAACCCTCG
  81	GAAAGTTCATGGAATCGAGC
  82	GCAAGGATTTCGACTCAGAC
  83	CAAAGAATAATCGCTCCTCG
  84	TAAAGCACTTATGACTCGGC
  85	TTATAGCATTCTGTAGGCGC
  86	TCGCTGACATTTGATTAGCC
  87	CCTTGAATAATATCTCGGCC
  88	AGGTCCAGAAATTGCTGCAC
  89	AGCTCAGGAAATTCTAGCGA
  90	AGCTATGCAAATTAGAGGCC
  91	GGTAGGCTAATTTATGGCAC
  92	CTAATGCAATTCAATGCCGC
  93	CAACTGGCAATCAATACGCT
  94	CCAAGCGAATGCAACGTATC
  95	GCATAGCGAATTGGAGATAC
  96	GCATGTCGAATGGATGATAC
  97	GCACGTTCAATGGCTCGACT
  98	GCAGCGCAATCTGTCGAGTA
  99	AGCAGTGCAAATCCTGATAC
  100	AGCTTCGCAAATCTGGTACA
  101	AGCCTGCGAAATCTACTGAA
  102	GCAGATCGAATTATGGAGAC
  103	GCAGAGTCAATTATCATGCC
  104	CGTTAGGCAATACATTTCCC
  105	ACTGGTGCAAAGTCTTCGAC
  106	GGTATATGAATGTGTCGTCC
  107	GATAGTGCAATCTAGGTGAC
  108	GCAGTGCAATGGATGTACTA
  109	GCTAGGCTAATGTCCGGCTA
  110	GGTAGCCTAATGTGTGCTCA
  111	GGACGTGCAATCTTGTGACC
  112	GAGCGCCGAATCTAGTCGAA
  113	GGGAGCGACCTCTAGCTTAT
  114	GCGGGTCGAATCTCGCTTAA
  115	CGCCGCGCAAGCTGTATTAA
  116	CGGCTGCGAAGCTGTCTTAA
  117	CATCCGCTAAGATCGGTTAA
  118	CGTGCAGCATAATCCATCAG
  119	TGAGAGCTGGATCGCATTCC
  120	TAGGTGCTAGGATCTCAGCC
  121	TAGGTATCAGGATTCAGGCC
  122	TGCGCCAGTGAGTCGTATAT
  123	CAGCAACGTGGATCAACTAT
  124	CAGCGGCTAAGATCAATACC
  125	GCAGCCTAATCTGGCCTAGT
  126	GGGCCTGTACCTGCAATTCA
  127	TAGGCCGGACCTGCTGTTAT
  128	TAAGCCGCCACGGAGTGTTA
  129	TAAGGCTCTTGAGACGTAGT
  130	TAAGCCCGATCAGCATGGAC
  131	TTGCCCGTAGTCAGCTTAGA
  132	GAAGCACCGATCAGACACTG
  133	CAGGCACCAAGTAGCACAGT
  134	GGTGCGCCATGTACTCAGTT
  135	TCAGGCTTATCGAGCGCGTT
  136	GCAGGCAGATCGACCTAGTT
  137	GGATAGGGACTCAGATATAC
  138	GCATGGTTACCTACGCCAGA
  139	GGAGGCTGACTCATACGCAA
  140	GGAGCCTGACCTAGTCGATA
  141	GCGGCCAATTCGGCGATAAT
  142	GGTGCTCGACATTAGGCCAT
  143	GATCCCACATAGCGGACAAT
  144	GATCCAATCTGTCAGCACAT
  145	GAGCCAATCTGACTACCAGT
  146	TGCTGGATATGACTGTCGTA
  147	TGCTCTGCACTGCTGACGTA
  148	TCACCAGCCAGACTGTGTAG
  149	AGGAGCAACCATCATGCACG
  150	GGGCATACCTATCCCGAGAT
  151	CGGGCGATACCACTCAGATT
  152	AGCGGCAACCAGACATACGT
  153	CACGCCATACCAAGGAGAGT
  154	CAGTGCATACCAAGCGACGA
  155	CAGGCAGTACACAATCTACG
  156	TACGTCGCATCCATAGCTGA
  157	GAGTGACACCTCAGCAGATA
  158	CTACAGCACCTCAGGAGAGT
  159	CTCACGACATCCAGGAGTAT
  160	CCAGCACGACAGAGAGATGT
  161	CGCACACACCTGAGAGAGAT
  162	GCGCACGCACTCAGATGTAA
  163	AGACGCTCAACCACGAGAGT
  164	GACGCCACAGTCACTAGAGA
  165	GGCGCACACTGTACTCAGAT
  166	CGAAGCGCCAGTACCAGATA
  167	GGGTCGCTACCTACTCTGAT
  168	GAGACATGATCTACCAGTAC
  169	GGACGCTTACTCAGCAGTCA
  170	CGGGTGTTACAGAGCTATCA
  171	CGCGGCTTACACAGACATTA
  172	CGGAGCTTACACATTAGCAG
  173	CTGAGCATACACTTCACGAT
  174	CCGATCATAACTGTAGATGC
  175	CCGCCGATAACTGCTTGAGA
  176	GGCCATATACGAGATGTAGA
  177	CGTCCCTTAACGGCTGGTAT
  178	ATACCCAGAACGACTATGCG
  179	ATCCCACGAACGATGAATCT
  180	ATCCGCAGAACCGGCGATAA
  181	CCTCGCCGAAGCGTGTTTAA
  182	GCGCCGCACAGAGTCTTATA
  183	CGCGCTGCACAGAGCATATA
  184	CCGCTGACACAGGCAGATAT
  185	GCGTATGACCAGGTGTATAT
  186	CTGTATGAAGGTGCTGTACT
  187	GTTTCGCACGAGGATGTATC
  188	GTGCTCGCAGAGGATTTATC
  189	TAGGCCAGAGTAGCGACTTA
  190	CAGATCCTAAGAGCAGTTAC
  191	TAGATGCTAGGAGCGATTCA
  192	TAAGTCGGTGGAGCATATCA
  193	TAAGCGCGTGGACTCCTAAA
  194	TAAGTGGACTGAGCGCATAT
  195	TATACGGCAGTGGATCAGAT
  196	CTATACGCAATGCACTCAGA
  197	CTATCGTCAAGTGATGGACC
  198	TATAGACTAGGTGATCGAGC
  199	TAGTACGAGTGGGCATCAAA
  200	TAGACGTAGTGAGCATGACT
  201	TGACGAGTTAGGATCTATGC
  202	TTACGAGTGTAGCGTCCATG
  203	TCGTCGTAGCATCTCGCAGT
  204	TCGACGTAGGATCGCAGTAC
  205	TCAGTATCATGGAGTACGAG
  206	TGCACTAGATGGGATCGACT
  207	TGCGATTACTGCCGTCACGT
  208	TGGACTCTATGGCAGCCGTA
  209	TGACAGCAGTTGCAGTCCGT
  210	TACACAGGCTTGCAGCTCGA
  211	TGCAGCGGAGTGCCTCATTA
  212	GCGCAGGGAGATCCATATCA
  213	CGGCAGCCAAGTCCAGTATA
  214	CAGCGCCCAAGACGTGTATA
  215	GTGCCTGCATAGCGATAGTC
  216	TGCCTGCGAGAGCCTGTATT
  217	TGGCATCGAGAGCCGTTCTA
  218	GCAGGAGCAGAGCTTATATC
  219	GCGGGATCACGACGTTTACA
  220	GTGGCGATAGAGCATTCTCC
  221	AACGCGAGAAACCATTTGCC
  222	AGGCAGACAACTCAATCCGG
  223	AGGAGAGCAACCTACACTCG
  224	AGCCAACGAACCTACATGGG
  225	CCGCAAGCACGTCGAATGAA
  226	GCGCATGGACGACAAACGTA
  227	GCCAGGAGACGTAGATATTA
  228	GCGCATAGAGAGAGATCATC
  229	TGGTATATCGGTAGATTCGC
  230	GAGCTATAAGGTGGATTCAC
  231	CGCGGATAACTTGATTCACC
  232	GTCGGCTTACCTGATAGCGA
  233	GGAGCTATACATGCCTATCC
  234	GGTGCCGTACATGCTCGTAT
  235	TCGGCTTGACGTGCTCGTAT
  236	GGGCTGTGACTAGACTCTCA
  237	GCGAATTTAGTAGACGCACA
  238	GAATCTCGAATAGCGGTACA
  239	GACAGTTGACATGACAGTAG
  240	GACATTGACATCGCATACAC
  241	GAGTTTAGAATCGTGAGCAC
  242	CTATTCGCAAGTGTCGAGCC
  243	GTTATGGACACTGCTCGACG
  244	AGCGTTCTAAATGCGTCACA
  245	CCGATATGAACTGTCACTAC
  246	CGCGAATGAAGTCTACATAC
  247	CCACTATGAAGCGATATACC
  248	CACCAGTGAAGAGATACCGC
  249	GCACTGTTACATGATACCTC
  250	GCCAGTTACAGTCATGCCTA
  251	GCGCAGCTAGATCCACTGAT
  252	GCGTGCGGAGACCTCATTTA
  253	GCTCACGAGGCACGCTTTAT
  254	GCGCCAGTAGCACGCTTATT
  255	GGCTCAGTAGCACTCATCAT
  256	ACTTGCACAGCACAATACGT
  257	CGCCATACAGCACGATATTA
  258	CCGCAGACAGCACGAGTATT
  259	CCAAGGAGACTACACGATCT
  260	GCACAGGTAGCTCGACGTAT
  261	GTCAAGATGCTACCGTTCAG
  262	CGATATGAAGCTCAGTGAAC
  263	CCTATGAAGCTATCGCAACA
  264	CTTATCACAGCATCCGAGAG
  265	CCCGTGCAACGATTTGACAA
  266	CGGCGGTTAAGTTCTAATCA
  267	GGTCGAGCATGATAGCTTAT
  268	GTGGTAGCAGCATAGCTTAT
  269	TAGCGTGGAGCATCCTCAGT
  270	CAACGGTGAGCAACTATCAG
  271	CTGGTTCGAGCAATCTATCA
  272	TCGGGTCTAGGATGCTCTAC
  273	TCGATGCACTGATGTCACTA
  274	TCGTATATCCCATGCGATCT
  275	TACGGTCCAGCATCAGCTTA
  276	ATCAGTCCAACCTACAGATG
  277	ATCAACTGAACCTCATACGG
  278	TACTTCTGAGCAGGGAGCTA
  279	TAGTTATGAGCAGGCGTCCA
  280	CTTGTGACATCAGCCACGAT
  281	CACGGAGCAAGAGCACATCT
  282	CACGGGTGAAGAGCCATACA
  283	CAGGAGTTAATAGCTCATCC
  284	TAAGATTAGTTAGCAGCGCC
  285	GAGTGATTAGCAGACGCCAC
  286	CGATGATTACCAATGCCACG
  287	GACTGATTAGCACATCCACA
  288	GATTATGTAGCACTATGCCC
  289	GCTATATTACGAGCTATGCC
  290	GTTTATATCGAGGCAGGCCA
  291	GTTACTATCCGATCAGAGCG
  292	CGTCATGTACCATCAAGTCG
  293	GTTATCTACGGATCATGCGA
  294	CTGCCGTAAGTCTCATGCGA
  295	CTAGCCGAATACTGCATACA
  296	CTGCGTCGAGAATCGCGTTA
  297	CATACACGACAATAGCTTCG
  298	GATACCGACTCATACATTGC
  299	GATACCGCACGATCAGCAGA
  300	GTATATGCAGACTACTGGAG
  301	TATAGTCGATTATCCCAGCC
  302	CATAGTACAATATCCCGACG
  303	CTTGACAGCTACTACCAGTG
  304	CTGAGACAGCTATCGACACA
  305	CTGAGTAAGTCTTCCACACG
  306	TCGGATATACTATGCGTCAG
  307	CGTAGGATAGAATGCACAGT
  308	CATGATACACACTCACGAGG
  309	CGGAATCACGACTACATACG
  310	GGGTATCACGAGTCACCTCA
  311	GAGAGAATCGTATCACAGCC
  312	GAGTATGTAATCTACCTGCC
  313	GAGTAATCATAGTAGCAGCC
  314	GACTATATCCAGCACCGAGG
  315	GACATATAGCTCCACTCAGA
  316	TAGACCTAGTTGCAGCGCGA
  317	TACTACACGTTTCACGGCAG
  318	GTACATATCTGTCACGCGCA
  319	TAGTATATCCTACGCCGCTA
  320	GAGTATATCGCAATGCCAGC
  321	GAGTTGTCACATAGGCCACC
  322	GACGCATGACATATTCCTAC
  323	GAGACACTTGACAGTAGCCA
  324	GGCTAGTTACTCAGATCACA
  325	CGCAATAAGTCTAGCTCACT
  326	CATGTACTAAGCAGTCACAC
  327	CTAGTTAATGTCAATCCGGC
  328	GACTGTGTAATCATTGCAGC
  329	CGTTCGTGAATCAGCACAGC
  330	ATTCGGTCACACAGCACAGA
  331	ATCTGCTGACACACACTAAG
  332	AGCTCGCTAAATATGTAGGC
  333	ACTGTCGCAAATATCACACG
  334	ACTGTCTGACCAACCAATAG
  335	GTTACTAGCTGGACCTCAGA
  336	TTATAGACTGGTGCGGAACA
  337	TTAGCATACTGTGCGCGAAC
  338	TGTGCTGACTTAGGTCGAAT
  339	TCTCGGGACGTTGCGCTATA
  340	TGTCCGCGACGTTGGCTATA
  341	TGTTCGTGACTGTGCGCTAC
  342	TGTCAGGTACTGGTCGCTAC
  343	TTCATGTACTGTGGCTACCG
  344	TTTACTAGAGTGGCGCATGA
  345	TTAGATAGATGTTCGGCCAG
  346	CTCAATAGATTATAGGCGCG
  347	TCGAATCGCTGTTACGGAAA
  348	TCAGACTAGGGTAGCGCATA
  349	TCAGCAGTATGTAGGCAGTA
  350	TAAGCCGGGTCACGCTATTT
  351	TATGACCGATGTGCAGGTAT
  352	TTAGCACGCTCGGCGATGTT
  353	TTCACACGGTCTGCGAGCTT
  354	CTTCAGACAGGAGGAGATAT
  355	TCCAGCCGACGTGCGATTTA
  356	TCCAGCGTACCTGCTTGTAG
  357	CTCCAGTCAAGTGCTTCGAG
  358	CTCCAGCGAAGTGATGAGAA
  359	TGTCAGCGGATCGCCATATA
  360	TCCATGCGAGGATCAGGTAT
  361	TGCAAGCAGTTCTCAGCGTA
  362	TGTAGGACCTGTGCTCACTG
  363	TTTATCGCAGTGCTCAGGCT
  364	TATGTCAGCAGGCCCAGCTT
  365	TTCTCGTAGCTGCGCCTAGT
  366	TATTCGAGCTAGGGACGCAT
  367	TATTTATACTGCGAGCGAGG
  368	GACCTTACACTGGCACGAGA
  369	TACTGATAGCATGGGACGTT
  370	TCGGATAGCAGTGCGCTCTA
  371	GCTGATGCACGAGGCCATTA
  372	GCTGGATCACGAGGCTCATA
  373	CGCTTTGTACCAGGCCATAG
  374	CGTGATTGACCAGACCCAGT
  375	TACGCTGGATCAGACGGTCA
  376	ATCCTGAACGCAGAGACACG
  377	ATCGTTGCACCAGAACTACA
  378	CTCTCAGGACCAGCATGATA
  379	TCTGAGCGATCTGCCAGTCA
  380	GGTGAGACCTATGTATATCG
  381	TTAGAGTCTTAGGCATGTCG
  382	TTATAGCCGTAGGCAGGTAC
  383	CTCTAAGTATTGGACACGCA
  384	GCTAGGATATAGGACACTGA
  385	GCTATCGAATGTGCAGTACG
  386	TCTATCCACTGCGGACGAGT
  387	TCATACTCATGTGCAGCTCT
  388	TCATCGAGATCGGCCACTGT
  389	CTTATGATACCAGTCAGCAC
  390	TATTGGTACGGAGTTAGCCC
  391	GTAGATGACCCAGTTCCAGC
  392	GGCTGTTACCGAGTCTCAGA
  393	TGCTAGTTAGGAGTATCGCA
  394	GGCTTACTAGCAGTCACGCA
  395	CAGCATATAAGAGTCGTACC
  396	GGCATCATAGACGCTACGCT
  397	GAGTCAGCAATCGCAGCTAA
  398	GATCAGTAATGCGGAGCAAC
  399	TATCATAGATGCGGACGGAT
  400	CAGTCCACAAGCGCGAGTAA
  401	CGTAGCCCAAGTGCCGATAT
  402	GACGCACCACAGGCTAGTAT
  403	CTAGCATACCAGGCGAGAGT
  404	AGTGCATCACAAGAGACTCG
  405	GCCATAGACGAGGCAGTATC
  406	GGAATACGCTGAGATATACG
  407	GTTAATCGCTCAGCAGCATT
  408	CACAAGCGACCAGAAGCGTT
  409	TCTTATCGACCAGGGCGGTT
  410	GACACTATCCCAGACGGAGT
  411	TTACTAGGTTCAGCGCGATC
  412	TTCAGATCCTCAGCGTAGTC
  413	TCTCAGATATTCGTAGCAGC
  414	TGTCTATTAGTAGCTGCGAG
  415	TAGATACTCTGAGCTAGGAG
  416	TGTCTCCAGATCGTGCGAGT
  417	TTCGGTCTAGCTGGTAGCAT
  418	ATCTGGCGAACAGGTGCATA
  419	AATGCGCGAAACGGCGATAC
  420	TTTGTCGCAGTAGTCGCATC
  421	TGTTGTGCAGTCTCCAGGCA
  422	CATTGTGAACTCTACGTCAG
  423	CGGATGTCAAGCTCTCACAG
  424	CTGCGGCAATACTCTCAGGT
  425	ATGCGGAGAACCTCTGACAA
  426	GCGCGTGAATCCTGTGACTA
  427	GCGCTCTGAATCTGTGAGAA
  428	GCGCTATGAATGTCAGCTAA
  429	GCCGAGGTAATGTGATATAC
  430	GCCGCGTGAATATGAAGATA
  431	GCGGCGAGAATCTTCCGATA
  432	GATGGTAGAATCTCTCTCAC
  433	GCTGCGGGAGACTATCATCT
  434	GCTGGATTACGATGCCATAG
  435	GTTGATTCACGATGGCAGAT
  436	CTTCACGCAAGTTGTCCAGA
  437	CTTACGCCAAGTTGTCAGAA
  438	CTTGCGTCAATAGTCTGAGA
  439	CCTGTGCGAACTGTCTTACA
  440	CTCAGTCCAAGTGGCTCAGA
  441	CCATAGCGAAGCGCACAGTA
  442	CCAGCACTAAGCGCAGATAG
  443	CTCCGCCTAAGTGGCAGTAA
  444	TGCGCCTGACGTTCGGATTA
  445	TGTCCAGTAGCTTGAGAGTC
  446	GCTCACAGAGTTTGATAGAC
  447	GCTACAGGAGTGGATATTAC
  448	GTGACAGTGGCAGATATAAC
  449	TCGCACTGAGCTGTAATCGA
  450	TCTTATGAGATGTAGCTCGC
  451	TCCATCTAGCTGTAGCCGAA
  452	GTCATAGCAGCTTAGACCTA
  453	TTATGCTGACTGTGCTCGAC
  454	TTAGTGCAGTATTAGTCGCG
  455	TGTCTGACCTTGTAGCCGAC
  456	TGTTGACACTTGCGTACCGG
  457	TCTTAGCATGTGCGACGACG
  458	GCTAAGCTCTTGCACTGACG
  459	CATAAGACTTTCCAATCGCG
  460	CTGAAGCACTTTCCACGAAG
  461	CTGAACCCGTTGCAGAGAGA
  462	CGGAACCGATGGCACAATAT
  463	GGTGACCGATGGCTACTCAT
  464	ATGGCGCGAACCCTGTACTA
  465	CATCGCGGAAGCCACGTATA
  466	GACGGCAGAATGCAGTATAT
  467	CGCGGAAGAAAGCATATTTG
  468	CTCAAGGGCACGCAATCTAG
  469	TCACAGGAGGCTCGACTCTA
  470	CGACAAGGCATTCACACTAG
  471	ATAAAGGTCATGCCAACCGC
  472	TATAATGCGTTTCACGTCCC
  473	TCTAATGCCTGACACGAAAC
  474	TGAATGCCGTGACTCGTAAA
  475	GTGGAGGCACTGCATCATAA
  476	GTGGTGTGACCTCGCCATTA
  477	GGAGATGCACTACGGACTAT
  478	GAGGATCGAATACTGTCGTA
  479	CGGAGAGCAAGTCATACGAC
  480	GCAGGAGACGGACTATACTA
  481	GAGCGTGTAATCCGATCTAA
  482	CGATACGGAAGGCGCACTAA
  483	CGATAGGTAAGGCGACTCAA
  484	GATGTGGCACGACGATCATA
  485	TGAGTAGGCAGTCCGATCTA
  486	TGATAGGCAGTGAGTTCATC
  487	TTATGGCGAGAGTTGTCATC
  488	GTTTAGGCACGATGCTGTAT
  489	GCGTTAGGACCATAGTCTAC
  490	CCGATGCGACAATACGTTAG
  491	TCTAGCGTCCCATAGCGTAG
  492	CTGTCTGGACCATAGCAGCA
  493	CTGCTTGCACGATGAGCGAA
  494	TAGCCCGGACGATGTAGTCA
  495	CCGCTACAAGCATTGGGAAT
  496	CGGCTAGAAGAATGAATGCT
  497	CCGATGATAAGCTAGTATGC
  498	GCGGATAGACCATTATTGAC
  499	GCCACTAGACCATCGGTGAT
  500	GCACGCGGACCATCGTTTAT
  501	GCCGCTCGACCATAGTGATA
  502	GCCGAGTCACCATGCTGTAT
  503	CACGGGTCACCAAGCGTATT
  504	GACGGCGACCCAGGTTATAT
  505	TGTGCGTCAGCAGTTAGTAT
  506	GCTCGGCTACCAGTCGTTAT
  507	CGCTGGACACCACTGTGATA
  508	CGGTGGAGACCAGATTATAT
  509	CGCGGGACACCAGCATATTA
  510	GCTCGCGCATTAGCATATAA
  511	GCTGACATCCACGCATTGAG
  512	CGCTGATCCACCGAGATTAG
  513	ACGCAACCAACAGCGAGTGT
  514	CACAGACCACAAGCTATGGG
  515	CCTAGCCCAAGGCATTAGAA
  516	CCGTAGCTCCAAGGCATGTA
  517	CAGTGCGCCAGAGCAAGTAA
  518	GAGCCACCACGAGTCATGTA
  519	GGTCACCACTCAGCGATGTA
  520	GTGTGCCACTAGGCCGATTT
  521	GGAGACCCGTAGGCATAATT
  522	CGCTGTAAGGATGCTGAATA
  523	GTCGTGCAGGATGCCATATT
  524	GTTCCGCACGATGCCAGATT
  525	GCTGCGACCATCGTCAGATA
  526	GTCTAGCGATCATGCTCAAT
  527	CTCTACGAATCATGCGGAAG
  528	CTTAGATACTACGAGCACGA
  529	GTGACGCTACGTGAGCCTAA
  530	TACCGTGTACGTGAGCGCAT
  531	TACTGCGACGTAGCGAGTCA
  532	TACTAGGTACTCGCGGCACT
  533	TACTGCGTACTCGGAGCATA
  534	GCTCACGTACTCGACAGAAA
  535	GTGTACTATGTAGCGAGATC
  536	TAGTAGTACGCTGTCAGAGC
  537	TGTCGTCGAGTCGTAGATAC
  538	GTAGTACACGGAGTGATCCT
  539	GTAGTACGAGCTGAGACTCT
  540	GTGACTAGCTCGTAATTCTG
  541	GAGACACGGTACTAGAGACT
  542	CAACAGCGTCACAGACATGG
  543	CTATGAGACCACCTCGATAT
  544	ATTCGGCGACAACGCATTTA
  545	GTTGCCGTACTAGGGATACT
  546	GGCGCAGTACGATTGACTAT
  547	GTGCGACGAGCTTGTCACTA
  548	TGCGTGTGACTATTGATACG
  549	CGTCTGCGAACTTTGCTACG
  550	CTGTAGCGAAGTTCTCATAC
  551	TCGGCGTTACGTGCTGACTA
  552	TGAGCTATACTCGTCGTCAG
  553	CCGATACTAAGCGTTACGAA
  554	CGTCATACATAGGACTAGCA
  555	CGCACGCTACAGACTATTAT
  556	GCGAGCGTACTATACATAAC
  557	GCGAGTCTACGACCTCTATA
  558	CGGTACGCACGACAGTCATA
  559	CGGTACATACGACTATACAG
  560	CGCTAGATACACCACTGATA
  561	CTCTAGGTACACTACTGCAT
  562	CGTCAGAGACACTGGAATAG
  563	CTGCGCGTACACTCGGATAT
  564	CTGTCGCTACACTCGTGAGA
  565	GTAGACGCCTAGTCAGATAG
  566	GAGCGACTACGAGCCACTAT
  567	GTGCGACTACGTGCATCACT
  568	CGTAGGACACGAGCGTATAT
  569	GGCGACGACGTGACTATACT
  570	CGGTCACGACGACGAGATAT
  571	GCGTCACACGAGCCGATATT
  572	GTCGCTCACGATGCGGATTT
  573	GACCGACAGATCGTGACATC
  574	GACCACGTACATGAGCTGAC
  575	GGCGACGTAGATGATATTCT
  576	GAGACTGTAATCGCATATCC
  577	GACTATGTAATCGAGCCTAC
  578	GATAGTCGAATCGCGGATAA
  579	TATACGGACTGCGCCCTAGA
  580	TAGTCTAGCTGAGCCATCGA
  581	GTATATGACCTAGTGCCACG
  582	GTGTTGTACGATGTGCTCCA
  583	GAGTCTGACATAGGGCACCT
  584	GAGTTGCACGTAGACGATAC
  585	GACTCGCGCATAGACACATG
  586	GACAGGCTACGAGACTAGAT
  587	GTGACGGCACTAGCAATATA
  588	CTGCTCTGACACGCGAGTAT
  589	CGGCTGTGACACGAGCTATT
  590	CTGGTGCGACACGCCTATAT
  591	GTCAGTGGACTAGCCCTACA
  592	ATCGAGTCAACCGGCCTAGA
  593	TCGATAGCCTACGTGCCGTT
  594	GGAGACCTCTACGCACTGTT
  595	GCGTGACAGCTCGCACTATA
  596	GCGTAGCTCAGCGACATTAA
  597	GCTATACGCACCGTCATGTA
  598	CGCATACACTCAGCAGAGAT
  599	CTACTTACAGCAGCGACGAG
  600	ATCTCGACACAAGCTAATCG
  601	CATCGGATACACGCATACAG
  602	ACATACAACACCGCTTAGGG
  603	TACTGAGTCCACGCTCGGTA
  604	GATACAGCCTACGACCGGAT
  605	GATACATTACTCGACACGCG
  606	CGCTACAGAGATGCACAGAG
  607	CCGACTGTAACTGCGATGAA
  608	GGTGTTATACGTGCATAGCC
  609	CTCGTATTAAGTGCGCTACC
  610	TATAGTATCGAGGAGCGACC
  611	GTATAGTACGTGATAGGCTC
  612	GTACGATACGTGACTAGAGC
  613	GTAGGTCGAGCTGCATACTC
  614	TTACAGTAGTCTGCATCCCT
  615	CTAGTCAAGTCTGCATACAG
  616	CTGTCTAATACGGCCACATA
  617	CTCGCAATACGTGTACCGTG
  618	TCCGATCTACGTGACGGTGA
  619	TCTCGCCGACGTGGTCTTAA
  620	TCTGTCCACGTCGCGGTTAT
  621	TCGTCCTGACTCGCTGGTAA
  622	GTCCCTAGACTCGCAGTGAT
  623	GCGACAGTAGCTGCAATGAT
  624	GACGTAATATCGCCACATCA
  625	GACGAGGTACAGCGCATACA
  626	GCAGGTCTACGACGCATGAT
  627	GCAGAGTACGGACGCATATC
  628	GAGTAGATACAGGTCACGAT
  629	GAGCGATCACACGTCCGATT
  630	GGTCGCATAGACGTATCAGT
  631	GGTGTCTCACGAGTATCGAC
  632	GTAGGCTAGACGGTCCACTA
  633	GACGGACACTGAGCACATAG
  634	GACACCTATGTAGCAATGAC
  635	CACAGTACAATAGCACCTGG
  636	CACCAGAACGTAGGCACAGT
  637	CACTACTCAAGAGCCAGTTA
  638	CGCCGACGAATAGCCAGATA
  639	GCCGCACTACTAGCGATGAA
  640	GACCAGTTACGAGCAGCGAA
  641	GATCACGTAGGAGCACCGTA
  642	GTACGCAGAGGAGTCATCCA
  643	GTCGCTGACTAGGATCACGT
  644	TACGCAGACTCGGACTCGAT
  645	GTCGCTATATCGGACCTAAC
  646	ACTCGCATAAACGACAGTCT
  647	TGGAGTCGAGTAGTACATAC
  648	TACGACATGGTAGGACGCTA
  649	TGACTTCTACGTGGCGATAT
  650	TACGCTCCGAGAGGCGATTT
  651	CACCTTCGACGAGCAAGAGT
  652	TACGCTCGCTCAGCTTAGGT
  653	TACGGCATCGACGCTATTGC
  654	TACGGCGACTGAGATGCCAT
  655	TACGTGCTAGGAGATGTAAC
  656	TATCGTCTATCAGATTGCCC
  657	TATCGTATCCACGTTCCGAG
  658	GATCGTACATCAGTGTCCAC
  659	GAGTCTATATCAGTAGCGAC
  660	GTTAGTCGATCAGTAGAGCA
  661	GTCCTACGATGAGTGACGCA
  662	CGTCTTCTAAGCGTGCTGAA
  663	GTCTCCTACCGTGAGCAGTA
  664	ATCTCACTACAAGAGCCTAG
  665	CTGTGACGACCAGACGCTTA
  666	CTGAGCGTAAGTGATTGTAC
  667	CTCGTAGCAATAGATTTCCC
  668	CTACGTGCAATAGCAGCTCA
  669	CCGGCAGTACAGATAAGTCA
  670	CGCCGGATACAGAGTAATCG
  671	CTCAGCATACATAGTACAGC
  672	CCGAGCTTACAACGTGTGCA
  673	GACGCATTACCACTGGCGAT
  674	CAGGGTGTACCACGAAGCAT
  675	CGGTGTTTACAGCAATCCAT
  676	CTGGCTGCAATAGCGCGATA
  677	TGGGCTACAGTTGCGCTCAT
  678	TCTGGCATAGCAGGTGTCAC
  679	GGGATTCTACCAGTTCGCAC
  680	GAGGATGCAATCGTAGTCAA
  681	AGGGATAACCATGCACACCG
  682	CATGAAGACTTTGCACTACC
  683	CGCCGACCAATGGGCATATA
  684	CCCGAGCCAACTGGAGATAA
  685	CCCGCAGCAACTGGGATTAA
  686	GCCATAGGAGCAGCGATTTA
  687	CCGCTTGCAGCAGACGATAT
  688	CCGTTTGCAGACAGCCAGTA
  689	CCGTTTACAATGAGCACACA
  690	CGTTCTTTAATGAGCGACAG
  691	CGAGCCTTAATGACGCACAA
  692	GGCAGCATACTCACGATCAT
  693	CTGCGAGCAATCAGCCGATA
  694	CCGCAGCAAGCTATCGAGAA
  695	CGGCGTTCAAGCAAACCGAA
  696	CAGTTTACAAGCATATCCCG
  697	CATTGACGAAGCATAGTTCC
  698	CATAGTGCAAGCAGCGACAC
  699	ATCTGTGCAACCATAGTACC
  700	ACTTGAAATGAGAAGCCCGT
  701	CAGGAGAAGCGAATAGCCTC
  702	CCAGAGAGAGCAATATCCGC
  703	CAAGGAATATACAGGCCCGC
  704	CAGAACTGAATTACAGCGCC
  705	CATCAGACAATTACAGCTCG
  706	CACCCGATAAGAGCATACGG
  707	CACTCCAGAAGCACGATAGG
  708	CAGCACCGAAGCAGAAGTCT
  709	CAGATCAGAAGCAGGACGCT
  710	CAGACCATAAGCACAGGCGT
  711	ACAACACAAATGGCGCGGCT
  712	ACGCAGATAAATCACCTCGG
  713	CAAGACAGAATACTCTCCGG
  714	CACAATACAATAGGCTCGCG
  715	CAATAAGACATAGGCCGCCG
  716	CACAACGGATTAGAAGCGCG
  717	GACATGATATGAGAATGCGC
  718	AGCAAACTAAGAGCCGGGTC
  719	AACAATACAACCGTCGGCGG
  720	AAATAACTAACCGCCTGCGT
  721	CAAACACGAAGAGCCTGTCG
  722	CACTAATCAAGCGACAGGCG
  723	CATATACCAAGCTATCAGCG
  724	CACATTCAAGACGATCACGT
  725	CACCTATGAAGAGACTCACG
  726	AACTATATCAAAGCCCTGGC
  727	ACAATACCAAATGCGCCGGG
  728	AGAAACGCAAATGCCTCTCG
  729	CGAAAGCATAATAGCGGTGC
  730	GGCAGAATCTCGTGTACTAG
  731	GGTACATTATGCTAGAGAGC
  732	GATACATGATGATAGCAGCG
  733	AGAACAGGAACATCGCTGCC
  734	AGATAAGCAACATCCTGTCC
  735	CATAAGCTAAGATCCTGGAC
  736	ATTTAGCGAAGAAGCATGGC
  737	ATAGCTCAATCAACGATGCG
  738	TATATCGCATCCACTCTGGG
  739	CATCTCCGAAGCACATTGAG
  740	CATTCGTCAAGCACTTCAGA
  741	CATTATCGAAGCACGGTACA
  742	GATTCGGACAGCACGGCATA
  743	GCTCCGGCAGTCACGATTAA
  744	GACTGTCGAGCACCCATTGA
  745	GATCGTCGAGCACGCCTAAT
  746	GAGGTCAGACGACGCCTATA
  747	GCGCGTATAGCTCTCCATAG
  748	TAGCGAGTAGCACTTCGATA
  749	CTAAGTGTAGCACCACATCA
  750	GTAGATCGAGCAGCCAGTCT
  751	GACATAGACCATACCACGTT
  752	CGTCTTCGAGCAAGTGCAGT
  753	CTCTCCGGCAGCGATATGTA
  754	CCCTCAGCACGAGATATAAG
  755	CCCTTGCGAAGCATTGCGAA
  756	CTCCAGGCAATGAGAGCACA
  757	CCCAGATCAAGCGATGCAGA
  758	CTGAATCCAATGTACGTGAC
  759	CGGCATTCAAGGTAGCGACA
  760	GCCCGATTAAGGTGTGTCAA
  761	GCCCGATCAATGGCTGCATA
  762	CGCCATCCAAGGGCTGTATA
  763	CGGATGCCAAGGGCTTCATA
  764	GGTTGCGCCAGGTCATCTTA
  765	GGTCCGGCATGGATCACTAA
  766	GGCTGGCACATGATCGTATA
  767	TGGTTGCACTTGGATCGAAA
  768	TGATTGCCACTGCTCATACG
  769	TGTTGATCCATGTCCATAGC
  770	TTAAGGCACTTGATCTCAGC
  771	GTAATGCCCTGGACCGCAAT
  772	GTTAAGCCTTCCACGGCAAT
  773	GTTGCGCCATTGAGCCAGAT
  774	GTTGCCCACCTGAGACGTTA
  775	AATGCGCCACAAAGCGAGTG
  776	CACCGGCCAAGAAGTACAGT
  777	CATCCGCCAAGCAGAGTGAA
  778	CGTTGCCAATGCACGAGCTA
  779	GATGGCTGAATGACGTTTAC
  780	GATTGCCTAATGAGTCTGAC
  781	AATCAGCCAAAGATGTGGGC
  782	AATCATGCACAAAGTTCGCC
  783	ATTTAGGCAAGAAGCGCACC
  784	AATTGGCTAAAGAGCGCACC
  785	ACATTGGCAAAGCGAACTCC
  786	AATGGGAGAAAGCCGACTCT
  787	TGTGCTGGAGCTTCAGTCAC
  788	GTTGTGCAGGATTATCGACA
  789	GCTTGCAGACGAGTCATCAC
  790	GGATGGATACTAGCGACTCC
  791	GCTATGGCACAGGCATCTAC
  792	GGACTGGCACATCCCGTATA
  793	GGATCGGACCATTCTCACTA
  794	GGATGGCGACATGCTCACTA
  795	GAGCTGGCAATCGTCGTACT
  796	GGATGGCTACATGATCTGAT
  797	GGCAGCAATTCGGGCTAATA
  798	GCCTAGCAATGTTCCCAGAG
  799	GAGCGGCAATGATGATCCAT
  800	TGGTGCATAGCTGCGATCCA
  801	GGCTGCACAGGTGTATCCAA
  802	GAGATGCCAATCGGCCATAA
  803	TATATGGCACATCGTTGCGA
  804	TGATGCCCACGTCGTCGTAT
  805	ATTGATCCACACACAGTACG
  806	AGCTGATCCAAGCAACGTAC
  807	GTTGATGCAGATCGCGTATC
  808	TCGTGGGCAGATCGCTTCAT
  809	TGTGGCCGAGATGCCTTCTA
  810	TTTGCGGACTTCGCTATCAA
  811	TCCCATGCACCTGAGTGGAT
  812	TTTCATGGAGCTGTCGCGTA
  813	TTTACCTGTGGTGATAGCGA
  814	TTGTCATGCTGCCCAGTCGA
  815	CTTTCATGCAGGCAGAGCCA
  816	CCTTTAAGCTGGCACACGAT
  817	CCTATCAAGGATGCACACGA
  818	CCGTTCAGAATATGACACAC
  819	TAGGTCAGATCATGCGCGAC
  820	ATGTGCATACAAGCTACGAC
  821	CTGAGAATATGAGAGACGCC
  822	ACTCACGCAAATGAACGGCG
  823	CTTAGCGAATATGCGATACG
  824	ACTCTGATAAATCCGACACG
  825	ACTGTGCGAAATCCCAGACA
  826	ACTGATGTAAATCCACACCG
  827	ACGTGAACAATTCCACACTG
  828	ACTGCACGAAATCGACATCG
  829	ACTTCTGTAAATCGCAGCAC
  830	CTGTCTTGAATAGCGATCAC
  831	ATGCGGTTAAGCGGTAATAC
  832	TACGCTGAGTCATCCGAATA
  833	CTTGTGAGACACTCCGACAT
  834	CTGGTGACATACTATCAGAC
  835	CGTGCGTTAAGCTGTCGATA
  836	CGGTATCGAAGCTGTGCTAA
  837	CGCGTGTGAAGCTGCCTATA
  838	CCTAGTAGAAGCTCCACAGA
  839	TGTGTCGGAGTCGCCCATAT
  840	TCTGTCGAGGTAGGCCATAT
  841	GCTGTCGAGAGCGATCATCA
  842	GCAGTCGGACGAGATTCTAC
  843	GCGATGGTACTAGATCAGCA
  844	GTGTAGGGACTCGTATCACT
  845	GTACGAGCAGTTGAGCATAA
  846	GTCAGTCGAGATTCAGCAGT
  847	GTCGAGTCAGATGCACGTCA
  848	GTGTATCTAGCTGCACGCAC
  849	GTTGTCTTACGTGCAGTCAG
  850	TATGTACTCGTATCGACGCA
  851	TCGTGTCGAGTATCCGCAAA
  852	GTACGTTGACAGTCTGCACA
  853	TTCGTAGAGGTCTGCCAATT
  854	ATTCTGAGAGACAAGCCTCC
  855	ATTCTGACACAATCATCGCG
  856	ATTCAGAACTAATGCACCGC
  857	AGGTATGAACCATCGCACAC
  858	ATTTGATGAACTCCGCAGAC
  859	GTTTGCTGACCTCGCAGTCT
  860	ATTGCCGGAACGCATTATAC
  861	TGTGTGGGATCGCCCTATCT
  862	TTGAGTGAGCTGCGCTTATA
  863	TGCGTGCAGGTGCCACTAAA
  864	GTGCTGCATGAGCCAGTTCA
  865	GGCTCTACATGGCGATAGCA
  866	GCTCTCTAATTGCGGACACA
  867	GGATATAAGTTGCGGCACTA
  868	GGATGTAATGGTAGCTCCTA
  869	GGATGACGAGGTCTCACCAT
  870	GGATGCGACGATCTCGACAT
  871	CGTGATCGAAGGCTGCACAA
  872	CTAGATGTAAGTAGCTGGAC
  873	CGAATGAAGGATCGAGACCT
  874	CGGCCTGGAAGTCACTCATA
  875	GGCCTTGGACTACCGCTTAA
  876	TGCTTCGAGGGTCCCACTTA
  877	TGCCTGGTACTGTCCGACTA
  878	TGCTTGTGAGAGTCGCTACT
  879	ATGCTTGCAGAACCGTCAGC
  880	TGACTGTAGGGAGCCTCAAC
  881	TGCTTGGCAGGATGTCTTAA
  882	GGCTCCGGCATGAGTATATC
  883	TGCTTTGCAGTGAGGCTCTC
  884	CAATTTGGAACTAGCCTTCG
  885	TTTGCTGCATCCGGCCTGTA
  886	TTGGGCCACTGCGCTCTTTA
  887	TGTGAGCCCTTGGCACGTTA
  888	GGTGGCCCGATCACATTCAA
  889	GGCAGGGCACCTCAGTTTAT
  890	GGGTGGCCCATGCTATCTAA
  891	GTCTGGCCCTACCTATGGTT
  892	GCGGGCACACCTCTGATTTA
  893	GCGGGCGCACCATTCATTAT
  894	GGAGCCCACCATGAGCTATA
  895	GAATCTCCACCAGGCGGATA
  896	GGATACGTCGCTACAGTGAT
  897	TCGTATAGCTGTATCGACGG
  898	CTAACTAGCTGTAAGCGACC
  899	ACTAGATAACAGATGCGCCG
  900	CAACTATCATCAAGACGGCG
  901	CAACAGAGATGAAGCGCGTC
  902	CAACATATCATAAGCGCGTC
  903	GCAGATAGCATCATATACGC
  904	GCAGACTGAATTAGCTCTAC
  905	GTTAATTCATCTAGCGCGAC
  906	AGGAATCTAACCACGCGCAG
  907	AGACCAATAAGCACCCTGGG
  908	AGACAAACATTCACGCCGGG
  909	AGAATAAATTACTGCCCGGC
  910	GAGCACATATTATTACGCCC
  911	CAGAAGATAATATGCTCGCC
  912	GAATAGCCGATAATCTCAGC
  913	GAATAGCTTTACACTGCCCT
  914	GAATCACTCTGAATGAGCAC
  915	GGATCACACTGCCGGACTAT
  916	GGACCCATAGCACTCTGATT
  917	GAGGCATTAGCACCAGCTCT
  918	GGATTATCAGCACTCAGTAC
  919	GGGATCTCAGACGATGCTCT
  920	GGGTATATCAGCGGATTCCA
  921	GCAATTCGATCTAATGCTCC
  922	ACCAATGCAAATAGGCGGCC
  923	AGCAAATTAACACTTGGGCC
  924	GAAACAAGCAGATTTGCGGC
  925	TTAATTCCGTGATATGCGCG
  926	GGATCTAATGGTTATGACCG
  927	GCATGAAGTGGTGTCAACTC
  928	GCTTTAATGGTCGTGACGCC
  929	GCTTAGAATTTAGTGCAGGC
  930	GCGTCAGAATTTATGCCACA
  931	GCTAGATAATTTAGGCCACG
  932	GCTGATAATGCTGAGGACTA
  933	GCAGAATTGCATAGACGCAC
  934	GCATGATTAGCATAGACGGA
  935	CCAGCAATAGCAATCACGGG
  936	ATTGCACATTCAACTGACGC
  937	TGGCATTTACTTAGTGCGAC
  938	GAAGCCATATCAATGCTCAC
  939	GCGAGCAATTTCATGCCACT
  940	GGCCCAAGTTTGTGACATGA
  941	GGGCATAATGGTTGATACTC
  942	TTGGTGCATGGATCTCTCCC
  943	TTTAGGGCAGGTTAGCTTCC
  944	TTATCCGGCTAGAGTGCGTC
  945	TGATGACCTGTTAGCAGTAC
  946	GGACCATGTGCTACGCAAAT
  947	GTGAGCAGATTCAGCCAGAC
  948	GAGAGACCATGCAGCCGATA
  949	GCGTCGTCAATGTTGCCACT
  950	GGGTTAATCCCTGCCACGTA
  951	GTGCTGACATTCGCGCCATT
  952	GCCTGTAATCGTGGGCACAT
  953	AGCGCGTGAAATGCACATAC
  954	AGCGTCTGAAATGCTATCAC
  955	AGTGCGCGAAATGTTCTACA
  956	CGTCGCCAATATGATCGAAT
  957	CGCCACAAGTTCGAGCGATA
  958	GCCCTACAGCGTGAGCTATA
  959	TGTCAGTGATCCGGGACTAT
  960	GTTATCGCACCTGAGGCGTA
  961	GTTGTGACCTCTGAGCACGT
  962	GTTTCACGCTATGCGAGCCA
  963	GTTTACCGCTCTCCAGGGAT
  964	TGCGTACCTCCTGCATGGTT
  965	TGACTACCGTGTCGCATACG
  966	TGGACTACGTGTCTCGATAG
  967	TAGTGATACTGACTCATGGC
  968	CGTCTGATACAGCCCAGTGT
  969	GCCGTATCACGACGCTAGAT
  970	AGCTCGATACAACGCTAGAG
  971	ATCTACTTAACGCGCTACAG
  972	GACATCGTACCACTGCGTAG
  973	GACTCGTGACCACTCTGTAG
  974	GACTCGGACCATATCTACGG
  975	CACTACGCAAGACTATGTAC
  976	CGAGTCTCACAGCAATCTAG
  977	CGATCTAGCACGCAATATAC
  978	GACCAGCGACGACAGTAGAT
  979	CGTAGACAGCCACGCAGTTA
  980	CGTATGCTACCACCGATTAT
  981	CGTGCGATACCAGCGTAGAT
  982	CTCCGTACAGCAGGCAGTAT
  983	CTCGTCGTACAGCGATCAGT
  984	CTACAGATACGTCGAGAGAG
  985	CTACGCGACACGCATGAGAT
  986	TAGACGCTCGCACGGTAGTA
  987	GCCGCTAGACGACGGTATAT
  988	GTATCACTAGGACGAGGTAT
  989	GTACTCACAGTGCGAGAGCT
  990	CGACTACACAGCTCAGGATA
  991	CACCGACAACTCGTAGAGAG
  992	CGACCCACACTAGGAGAGAT
  993	ACGCGCACAACAGGAGACTT
  994	AGTACCACAACTCAGACGTG
  995	AGTACAGCAACGCAGAGCCT
  996	GTCAGCGACCGTCAGCTATT
  997	GTCAGGCACTAGGAGCTATC
  998	TGTCGGTCACTCCTGGACTA
  999	TCGGTTCACGTCCGCATGTA
  1000	TCGTTTACCTGTCGCGCTGA
  1001	TGTGTCTCACTTCCGCGAGT
  1002	TCTGAGCACTCTCTCGTAGG
  1003	GTTGATGACTCGCCACACGT
  1004	CTGAGATCACAGCAGACTAG
  1005	TTAGACTCCTCGCCGGTAGA
  1006	TATAGCTCCTAGCAGGCGTA
  1007	TATGCTCCACGTCTAGTGAG
  1008	CTCTATCACCAGCGATGAGA
  1009	CGCTCCAGACAGCATATAGA
  1010	ACATACCGAAAGCTCTAGCG
  1011	ACATCGCTAAAGCACATCGG
  1012	ATATCGCGCAATCAACGCTA
  1013	CGATGCGCCACTCAAGGTAT
  1014	TATGCCGACGGTCAGGCTAA
  1015	TATCGCCACGTCCGGTGATT
  1016	TCTCGCTCACTGCGTATGAT
  1017	TATCCGTCACTCCGTAGAGG
  1018	TATCGACTATCCCTGAGACG
  1019	GTATAGACCTCTCAGACGCG
  1020	CTATCGTAATATCAGTCCGC
  1021	CGATGACAATTAGGTACACG
  1022	GAGCATAATGACGTAGACCG
  1023	CGACAATACTTGACAGCACG
  1024	CGATGATAATAGAGTAGCCG
  1025	CTATGATTAAGTCGTAGCCC
  1026	AGGTGAATAACGCATACGCC
  1027	GAGTGAGTAATGCTACGTCA
  1028	GATCGACGAATGTTAGAGAC
  1029	GACTCACGAATGCGGAGACT
  1030	GACCGTCAATCGCGTCAGAT
  1031	TACCCGCATCGACGGAGTTT
  1032	GTCAGCGCACTCCTGGTTTA
  1033	TCAGGCCCACGTAGCGTTAT
  1034	TTCGCGCTATCCATGCGTGA
  1035	TGCTGATACTCGGCTGCATC
  1036	TGAGTAGCATCGGTGACTTC
  1037	TTGTATCACTGTGCTGCCCA
  1038	TTTAGTCAGTATGCTCGCGG
  1039	TTACGTTTATATGGCCGAGG
  1040	TGAGATCACGTTCGCCGAGT
  1041	GTATCATTAGCTCCGCAGAG
  1042	TATCATGTAGACTCGGAGGC
  1043	GTATGCTTAGATATGCAGCG
  1044	TTGTAGTTAGCTCTGCACGG
  1045	ATATCGTTAAGCCATACGCC
  1046	ATTCTGATAACGCTCTCGAC
  1047	ATTCGTCCAACGCGGTCGAT
  1048	ATATGCACAACGCGCAATCG
  1049	TTAGCTCTATCGCAGTCCGA
  1050	ATTAGCTGAACGCCTCGCAA
  1051	ATTATCTCAACGGAGGAGCA
  1052	ATGTTGCTAACGGACGGACA
  1053	ATGTGTTCAACGGAGACAGA
  1054	CTCTTTCTAAGTGAGTCGAG
  1055	CTGCTTGAAGTCGTCTCACG
  1056	CTGCGTTGAAGTGGCTTACT
  1057	GTGCGTTCACATGGCCGTAT
  1058	GTAGCCGCACCTGACTGTAT
  1059	GTAGCGCCACCTGACGTTAT
  1060	GGCGCGTCACATGATACATT
  1061	GGTTGCTACGATGACTCAGT
  1062	GAAGGCCCGTACACTCTATA
  1063	GACAGGGCACACGACTCTAT
  1064	TGCGCGGCACTCGTTCTATA
  1065	GCGGTTGCACTCGTAGCATA
  1066	GAGGCGTGACCAGTCCATAT
  1067	GGACGCTCACCAGTGCTTAT
  1068	AGTGTCCAACCAGACCAGAG
  1069	AGTGCCATACAAGCGCATAG
  1070	GTAGCCTTACATTGGCAGAG
  1071	GTCGCCGCACATTCGGTTAT
  1072	GTTGAGTCAGATTAGCAGTC
  1073	TCGTAGGGACTGCGCTCATA
  1074	CTCAGATGACAGCGACGCAT
  1075	CTCTGAGGACAGCCGAATCT
  1076	CTAGGATGACAGCCAGACAC
  1077	CGTGAATTACATCAGACAGC
  1078	CTGATTATAGCTCATACGCC
  1079	CTAATATGATGACAGTCCGC
  1080	TACTTATGATGACTGCGGAC
  1081	GAACTATGCTGACAGTACCG
  1082	CGATTCTGACCACATACGAG
  1083	CTAATCTGACCACGAGACGA
  1084	CTGTATTGACATCAGACGAG
  1085	CTTCTCAGACATCGGACGAG
  1086	GCACTGTGAATTAGCGAGCA
  1087	GCCTACGGAATTGGCAGACT
  1088	GACCTGGAATTAGCACACGC
  1089	GCCTGCGAATTAGCGGACAT
  1090	GCGATGCTAATGATGTGTAC
  1091	GCCCGTCTAATGAGTGGACA
  1092	GCCTAGCTCATCAGACGGAA
  1093	GCATGGACATCCTACGAGAA
  1094	CGCCTGCCAAGCTGTGATAT
  1095	GCCTGCGCCATCAGTAGATA
  1096	GCACGGCCAATTACTCGATA
  1097	GCAGCGAGACCATGTGATAC
  1098	GCAGCAGCACACTGATCGTT
  1099	GACCCAGCACATTAGCGAGA
  1100	GCTCCTGCAATGTGCGGATA
  1101	GCGCCTGAATTGTAGCACGT
  1102	GCCACAGCATTGGAGAGAAT
  1103	GCCAGGCTAATGGATAGTAA
  1104	GCCCTGCGAATGAAAGACAT
  1105	GCAGCGGGAATTAGATATAC
  1106	GCAGGTGCAATGATTCTACC
  1107	GACCGGGCAATCACTTCAGA
  1108	GCCGGGCAATGCGTTCATAT
  1109	CCCAGGGCAAGCGATCATAA
  1110	GCCACAGGCAGGGCATATTA
  1111	GCCTAATCCTGGGACACTGA
  1112	TCGTCTCGATCTAGGCCATG
  1113	GTGTCTCGACTCAGCCTATA
  1114	GACGTAGTAATCATGTCTCC
  1115	GACTTATACGTCATGCGACC
  1116	ACGATGTAACACAGCGACCG
  1117	AGTCGTGTAACCATGTGACA
  1118	GTCGTGACAGTGATGTACTC
  1119	GTGGAGTGACGTATCTCTAA
  1120	TAGAGGTGACGTAGTCCACT
  1121	GTCGTGCGAGATAGCTCTTA
  1122	GTGTAGAGATATAGCATCGC
  1123	TAGTCGTGAGATAGCGATTC
  1124	CAGTGTGTACGAATACGAAG
  1125	CGAGTGTCACATACCACATA
  1126	CGTATAGCAGACAGCGCAAT
  1127	GACATCGACGACAGGCCATA
  1128	CGAAGCTCACGTAAGTCAAG
  1129	TAGTGCTCACGTAGCCCAGT
  1130	TGCCCACGGTGAGCTAGTTT
  1131	TAGCTGCCAGGAGCGTTCTA
  1132	TCGGCCTACGCTGTGCATTA
  1133	TAGGGTACTGATGAGCACTC
  1134	CTACGGGAAGGTTAGCACCA
  1135	TGGTGATACCTGTGCGCCTA
  1136	GATTAGATACCACTGCCACA
  1137	GGAGTGATACCTCGATCCAC
  1138	AGCTGACGAAATCTTCACAC
  1139	GAGGAGATAATGGTCACTAC
  1140	CACGGAATAATACATCCTCG
  1141	ACAGCAACAAGTCGAGCCGT
  1142	ACGGAGAGAAATCAGCCCTC
  1143	CAAGAGATAATACGGCTGCC
  1144	CAAGTCCTAAGACAGCTACG
  1145	ATAAGCGCAAGACAGGCGTC
  1146	ATCTGAGCACAACTAGGACG
  1147	CACAGGCTAAGACAGGAGCT
  1148	CATAGCGTAAGCCAAGCAGC
  1149	CATAGTCTAAGCCACATCAG
  1150	GACAGTACATGCCAATCAGC
  1151	GCGGTAATCGGTGCATCAAA
  1152	GGGAGTATAGCTGACCATCA
  1153	GTAGGCAGACCTGATCCCTT
  1154	GAGCCAGACCACGCTTGATT
  1155	GGCGCATCACTAGCCAGATT
  1156	GGAGCTACATCCGCCAGTTT
  1157	GGAGTCTACCCAGGGCATTT
  1158	CGCGCTCTACACGATGGATA
  1159	CGTGCCACACCTTGGAGTAT
  1160	CGCGGCACACAGTTCAGTAT
  1161	GCTCGTCCACAGTGCGTTAT
  1162	GCTGACGCAGAGTCCAGTTA
  1163	CCGTAGCGACAATCAGCTTA
  1164	ACGCACCGAAAGTGAGCGAT
  1165	ACGTCCTCAAAGTGCAGACA
  1166	ACGCAGTCAAAGTCATATCC
  1167	CAGAGTCTAAGATCACCACG
  1168	CACTGTCTAAGATACACACG
  1169	CAGCGTACAAGCTATACAGC
  1170	CCGACGACAATGTACGACAG
  1171	GACTAGCGAATCTAATGAGC
  1172	CGTCGAGCAATATGAATGAC
  1173	CTGTCGCGCACTTCATAGGA
  1174	CCGCGACCACGATAGAGAAT
  1175	GGCACACACGTCTCGGATAA
  1176	GGCAGACGACGTTGCATACA
  1177	CGTGGGACACAGTCGATCAT
  1178	AGTGCGAGAACATCGTGTAA
  1179	GGCAGCACAGCTTGTACGAT
  1180	GACCATTGAATATGTCGAGC
  1181	GTACGCATATTTAGCCAGCA
  1182	GGCAATCTGTTCACGACCAA
  1183	GCTGACTAATTGCTAGACAG
  1184	GGTGTCTAATTGTATGCACG
  1185	GTTGACACATTGTTAGCAGC
  1186	TTAAGAGATTAGTCTGCCGC
  1187	TCACGTAATTTGTTAGCCGC
  1188	TGAGTGATAGCTCGGATCTC
  1189	ATGATGATAACTACGTGCCC
  1190	ATGCGAATAACTATGACGCC
  1191	ATGGAGATAACTATGCACCC
  1192	TCGTTGCGACCTATGCGTAG
  1193	TAGTTCGCACCTACTGCTAG
  1194	ATACGTGCAACCACTGCTAA
  1195	ATGTCGATAACCTCTGCTAC
  1196	ATCTAGTCAACCTGAGCTAC
  1197	AGTATAGCAACCTCAACTCG
  1198	AAGACACTAAACTCTGCTCG
  1199	ACGATAATAACAGCTCCTCG
  1200	ATAGATATAACTGACGCGCC
  1201	ACTGTAATAACCAAGCCTCG
  1202	ACTGATAGAACCACAGCGCG
  1203	ATGGCGACACACATACAGCG
  1204	ACGGCGAGAAATACGATGCC
  1205	GACGCGAGATCAATGTAGTA
  1206	CGAGAGTAATCAATCATCCG
  1207	CGAGCAATACATACATCTGC
  1208	CAACATAGTTACACACGCTG
  1209	CAGCTTATAGAGACACACTC
  1210	CCATAGAAGTAGACACCTCG
  1211	CTCAGAGACATGACACTCGA
  1212	ATCAGGTCAACTAATCACCG
  1213	AGCGCAGTAAATAGCTTAGC
  1214	ACTCCACGAAACATGATTGC
  1215	CTCAATATAGACACGATGCC
  1216	CGCATTAGAGACAGATCGAG
  1217	CGCACATGACATAGAGCACG
  1218	CGCACATTAGACAGAGAGGC
  1219	CTAGACTAATGCAGAGAGCG
  1220	GCGTATAGATGCAGAGATCC
  1221	TCACTAGCGTGGAATAGAGC
  1222	CAGACTGAACTCAATGTACC
  1223	CACGATGAACTAGATGTACC
  1224	CGAATGATAAGTATGACGGC
  1225	CGAGATGCAAGTATAGTACC
  1226	GGATAGCGAGATATAGACCC
  1227	GCATAGCACGATGGACGATC
  1228	CTCACAGGACATGCAATCGG
  1229	TATACATGCTTCGATCACCG
  1230	ATATCAATAACTGCGACGCC
  1231	AATACGAAAGATGCGGCCCG
  1232	ACAGATACAAATGTCGCCCG
  1233	ACGAATAGAAATGTGGCCGC
  1234	ACATTACTAAAGGTGCGACC
  1235	AGATTAGTAAATGCTGCGCC
  1236	ACTATGATAACAGCAGCCCG
  1237	ATATGAATAACTCCAGCGCC
  1238	AGACTGAAATCTACAGCCCG
  1239	GTACTGATAATTGGATCGCC
  1240	CCAGAACGGTTGCAGACACT
  1241	GCAATAGTTGGACCCAGGCT
  1242	GGAATAGGTGGACTCACTCA
  1243	GCACAAGTTTCGCGCATCGA
  1244	GCGGAATCTGTGCAGCATCT
  1245	GCGAGAATATGGTGACATCT
  1246	GCGGTCAATTAGTGGACTCC
  1247	CTCCTACAATGGTGACACTG
  1248	CTATTACAATGGTATGCCCG
  1249	AATCATACAAAGTGTGCCGC
  1250	CATGATCTAAGAGTGTAGCC
  1251	CAAGAAGTAAGATGCGTGCC
  1252	CATGTGATAAGATGTGGACC
  1253	AACTTAGCAAACTTAGCGCC
  1254	TCTTCGATATGATAGCGTCG
  1255	GACGTTAATTGATGAGACGC
  1256	GCGTGAAGTTGTTAGCACAT
  1257	GCCGATACATGCTGCACGAT
  1258	CGCCGATTAAGCTGCGACAT
  1259	CGTCATTTAAGTTAGCGCAC
  1260	CTCCATCTAAGGTGCGATAC
  1261	CGCTTATCAAGGTGCAGACC
  1262	GATGACTCAATGTGACTCAG
  1263	CGCTAGTGACAATTATGTGC
  1264	GCTAGGTGACAGTATGCTAT
  1265	GCTGTGCTACGACGTTGACA
  1266	GCTAGAGTAGACCGATGCCA
  1267	GTATATCGAGATCATAGGCG
  1268	GTCTTGGACTATACGAGCGC
  1269	TACTTGTAGATAGCGAGCGA
  1270	GTACTCTGACATGATTCGCA
  1271	TATACTGACCTTATCGGCAC
  1272	TCGTCTTGAGATATGTGGAC
  1273	TCATGTTACGGTATGCGAGA
  1274	TCATCTGCACGTATCGTCAA
  1275	GCGACTGGACAGATTGCATA
  1276	CGGGCGCGAAGTATTCACAT
  1277	GTGTGGGCACGTATTCCATA
  1278	TCCGGGCACGGTGTCATATA
  1279	TGGGCGCTACTGGCTCTTAA
  1280	TGCGCCGCCAGTCTGTTATA
  1281	TGGCCGTTAGAGTCTGCACT
  1282	ATGGGCGCAACCCTGTCATA
  1283	CAGCCCTGAAGACTGCGATA
  1284	CGCCGCTCAAGGCTATGATA
  1285	CGCTCCTGAAGGGTAGTTAA
  1286	GGCCCGACAGGTGCTATTAT
  1287	GGATAGGCAGATGCACTTAT
  1288	GGACAGACGTTGACCAGCTA
  1289	GTAGCGACATTGAGTTAGCA
  1290	GACTACGAATTGAGCATACG
  1291	CTACACTAATTGCAGCAGCA
  1292	CGTACCCGAATGCAGCAGAA
  1293	GACGCCTAATGACGCTGAAA
  1294	TAGCTTGTACTGCGACTGAC
  1295	GATACTCTAATGCCATCGAC
  1296	CGGCGTACAATGCCATAGAA
  1297	CGGATACGAAGGCTATGCAA
  1298	ACGGATCGAAAGGTATAGCC
  1299	ACGGCGCGAAAGCGTCATAA
  1300	CGTGAGGGAATACGTCATCA
  1301	CACAGTGGAAGACGCATCAC
  1302	GAGGTGACATGACGTACATC
  1303	GAGTAGCGAATGCTCAGCCA
  1304	TATAGCACAGTGTCCAGCAA
  1305	CGTATGTCAAGGGCCTGATA
  1306	CGAGACGCAAGGGATTTACA
  1307	GAGACGCAATGTGAATTACG
  1308	GATCGCACAGGAGCGTATCA
  1309	TGCCCAGAGCGTATGAGCAA
  1310	TGAGGGCGAGCTATCTATCA
  1311	TTGTGGCTAGGTATCGCTAC
  1312	TGGTTAGCAGGTATGATCCT
  1313	CTCACTGCAAGGATGGGACT
  1314	TCCTGTAGATCCCTATGCGG
  1315	TCGTTGTCAGCATATTGAGC
  1316	ATCATGTGAACCTATTGGCC
  1317	TACACTGGGACCTATGGGCA
  1318	TACCTGGGAGCATAGCTGAC
  1319	TAGCCCGCAGCATAGGGTAT
  1320	GAGCCTCAATGCTACGGAAG
  1321	GATGTTCAATGCTGGCCGAA
  1322	GACTTGTGAATATCTGTGCC
  1323	GCCGCCGAATTATTGAGCAA
  1324	TGGACTGATTGATAGGCAAC
  1325	TGGCAGATCGGTGTATTCAA
  1326	TATGCGTAATGGGTGTTCCA
  1327	TTAGGTCGATTGATAGTCGC
  1328	TCTGCTTTACTGCGTAGCCA
  1329	TTGACGAGTTTGCAGTGCTC
  1330	CTTGATTAAGTGCTGTACGC
  1331	CTCGGATCAAGGCTTACCGT
  1332	CCGGGCTCAACGCTTTGTAA
  1333	TGTCGCCCAGCTCATGTGTT
  1334	CTGGACCCACAGCTATGGAT
  1335	CACGGGCCAAGAGATATACC
  1336	CGCCCGCCAAGTGATGTATA
  1337	CGCCAGCCACATGGATAGAT
  1338	GCCCGGATACATGCGATTAG
  1339	GCTGGCCTACATCCGTATGA
  1340	AGATGGCGAAATCCGTATAG
  1341	GCAGGGACATTACGATCAGT
  1342	AGCAGGTGAAATCGTACTAC
  1343	GCAGGTCAATCTCTGTACGA
  1344	GCATTGTAAGTTCGGTCAAG
  1345	GCACTGGTAATTCAGCTACG
  1346	AGCATCATAACCCAAGCTGG
  1347	ACCAGTCCAAAGCATAGTCG
  1348	ATCATTTCAACGCAGTGACC
  1349	TCAGCCCTATCGCAGGATGT
  1350	GTCAGCACCAGCCGTGATTA
  1351	GAATTACGCACCCAGCTTGA
  1352	GAATGCGCCTACCAGCTATA
  1353	GAATGGCGACAGCGTACATA
  1354	GGATTGCCACGACTCACAAA
  1355	GCTCATTGACACTGCGCTAT
  1356	GAGCATGGACCACGGCTATA
  1357	CAAATGGACAGACAGCCTGC
  1358	CACTTTGAAGCACAATCACG
  1359	GCTGTTGCAGGACGCATCTA
  1360	TACCTGGCATGACGCGATAT
  1361	TTCGTGGACTTGCGGATCTA
  1362	TTCCTGCGATAGCGGCGTTT
  1363	TTGATCTGATAGCGGGTCTC
  1364	TTGATCGCATAGCGTCTGAC
  1365	TTCGAGGCATGTGGATCTCC
  1366	TTCAGCGGCTAGGCGATTTC
  1367	TCCAGCAGATCGGCGAGTTT
  1368	TTCAGCCGATCTGCCGATAT
  1369	TTCTATCGCATGTCAGCCGT
  1370	TGTAATGCCTGCCAGCCGTA
  1371	TAATTGCCTGCACAACTGGA
  1372	TAATTCCATTGACGGCAGCG
  1373	TTATTGCCATAGCGCGACGC
  1374	ACAATTTCAAAGCCTGACCG
  1375	ACAGGCCCAAAGCACTAGGT
  1376	CGAATGCCAAGGCCAGCTAA
  1377	GATGGTTCAATGCCTGGACA
  1378	CTGGGCCAAGTTCTGAGACA
  1379	CGTGGGCAATACAGTTGAAT
  1380	GAGCTGCGAATCGGTATTAA
  1381	GACCGGCGAATCGAGCATAA
  1382	GACTTCGCAATCGGCACGTA
  1383	GACGCGCCAATCGTGCTATA
  1384	GATCGCTGAATCGTGCGTAA
  1385	GATCACTGAATGCGACGTAA
  1386	GATCGTGCAATGAGGTTACA
  1387	GAGGACTAATTGAGATGCAC
  1388	GACCGATAATTCGATATGCC
  1389	TAGCATTGATCCCATGTCAC
  1390	TTCAGCTTATGCCAGTCGCG
  1391	TGACGGCCTTGCATATCCGA
  1392	GAACGCGCCTTACATCAAGA
  1393	GAATACCAGTTACACTCCAG
  1394	CAAGAACTGTTACACATCGC
  1395	GACGAGAATGGACTACACGT
  1396	TACAGACGCTTGCATAGATC
  1397	TAACGACCTTAGCGACGGGT
  1398	TAACGACGCTTTCCCAAGGA
  1399	TTACCGCTGTTGAGCCCGTA
  1400	TTCCATGTATCGAGCGTCAG
  1401	TATACGCCCTTCAGATCGGG
  1402	CTAAGCCTATGCAATATCGC
  1403	CCAGCTATAAGCATATTGCC
  1404	TACAGCATTGTCATGGACTC
  1405	TAAGCTATTGGACATTGGGC
  1406	TTAGCATCCTGTCATAGGGC
  1407	TCTAGCAGCTTTCATAGCCA
  1408	TCATCACGCTTTCCGAGGAT
  1409	GCATACATTGGACGAGAGCT
  1410	TCTAGCATTTAGCATGGTGC
  1411	TTATGACTTGATCTGAGGCG
  1412	TGTTCGCACTGGCTTAGCTC
  1413	GAGTTGAATGCAGATAGCTC
  1414	TGCAGGCTCGCAGATGCTAT
  1415	TGCGAGGACTGTAGCTTAAT
  1416	TGGGCACTCTCGCCTAGTTT
  1417	TGAAGCGCCTCGACTAGGTT
  1418	TCATCGGCACTGATAGCTCA
  1419	TCATCAGGCATGGAGCCAGT
  1420	TAATCAGCGTTACGTCCGCA
  1421	GAATGTGACGCAAGTCTGAC
  1422	AGATTTGCACAGATAACGCG
  1423	GATTACTGACCAGCATCGAG
  1424	AACTATCGAAACCGCCAGGG
  1425	ATAATACAAGAGTCGCGCCG
  1426	ATAATCATAACCTCGACGCG
  1427	ATTATCATACAAGGCAGGCG
  1428	TATATCGGATCAGCAGGTCA
  1429	TAATTTCGCTACGCAGGGAG
  1430	TAATCCTGTTACGCGGAGGC
  1431	CTTTAGCTCCACGCAGTGTG
  1432	TTCTAGCCGTCCGCAGTTTG
  1433	GTCATGCGAGCAGCAGTCTT
  1434	GGCGTTCGAGCAGTCATCTT
  1435	TACCGCCAGTCAGCGAGTTA
  1436	TACCGCCTAGCAGCATTGGT
  1437	TACCGCACTGCATGTCAGGT
  1438	TGTCTCGATGCAGGTCTAGT
  1439	GCCGCATGACGAGGATATAC
  1440	TACCGCGAGGCAGGATTCTT
  1441	TACAGCAGTGCAGGGCCTTA
  1442	GCAGCTAGAGCAGAGTATCA
  1443	GACAGCAGATCAGAGACTCC
  1444	TAAGCACGTTTAGAGCTGAC
  1445	TAACCGTGTGCAGATCGGAT
  1446	TACTGCGGACCTGGATCTAC
  1447	TCAGGGCTACTCGATTGGAA
  1448	TCCGCAGACTTAGCGTTACG
  1449	TGAGCAGCCTACGTTACTAG
  1450	TGCGTCAGATGCGTATATGC
  1451	TCGTCCAGATGCGGAGTTCA
  1452	TCGGCTATATGCCAGATCCT
  1453	AAGGACAAAGAGCGCGTCTC
  1454	TAGCACCGATGGCGAGCTTA
  1455	TGTCCACGGTGCCGCAATAT
  1456	TGGTCCGACTGCTGCTACTA
  1457	TGTGCCGACTGCCGTCTTAT
  1458	TTCGCAGTATGGATCGGTAT
  1459	TTACGCAGTTGCATGGAGCT
  1460	TTCTGATTAGCTGCGGACGC
  1461	TGGTTATACTTTGCGAGAGC
  1462	TTTGTTAGCTTCGGGCAGCC
  1463	TTGGTCTGATCCGGGCATAC
  1464	TGCTTGGACTCCGGCGATTA
  1465	CTGCTTGGACCAGCCAGTTA
  1466	AAGCTGGGAAACGCACACCT
  1467	AAGCGGGCAAACGATATGCT
  1468	AAATGCCGAAACCATCTCGT
  1469	CCATTCGGAAGCGACTCGAT
  1470	TACATGGGCTGAGAACGCAA
  1471	TATTGGGCACGAGCGCCTAT
  1472	CATCCGGGAAGAGTAGCACA
  1473	ATTTCATGCACATAGCACGC
  1474	ATTGCAGCACAAGCCAGACT
  1475	TTGCTAGGCTCAGTCCCGAT
  1476	TTGGCGAGCTGCGTTCTCAT
  1477	TCCCAGAGATGCGACTGCTA
  1478	TTCGCTGGATCGGCATGTCT
  1479	TTGCTCCTAGCTCGCGTGAT
  1480	TTGCTGCTAGTCCAGTAGGC
  1481	CATTAAGCAGTCGAGAGACC
  1482	CGTTAATGCAGCGAGAATCA
  1483	CGCAAGCTCAGCAGAATTAC
  1484	CCATGTCGAAGCATTCATAC
  1485	CTGAATGTAATCATCGTGCC
  1486	CTTAGATGAATCACTGCCAC
  1487	CTTCACGGAATCTAGGCACA
  1488	CACTCTTGAAGCTAAGCACA
  1489	CCTCTAAGCATGTTGACACA
  1490	CATGCCGGAAGATGCGTACA
  1491	CAGGCAGCAAGATGTACGAC
  1492	CAGTGGGCAAGATAAGATTC
  1493	CCGTGCCCAAGCTAGTGATA
  1494	GATCGGGCAATCTGCGTACT
  1495	TTCAGTGCATTATAGTGCGG
  1496	TTATCTGCATGAGTAGGTCG
  1497	TCGATAATCTTTGTAGCGCG
  1498	TCTTACAGCTTTGCAGGGAG
  1499	TCCTACATTTGCCACGGGAG
  1500	TCTTCATCAGTGAGGCGCGA
  1501	TTTCTAGGATGTATGCGAGC
  1502	TATCCAGCATTACTGCGAGA
  1503	TTATTCTCAGCACGCACGGA
  1504	TGATTCGCACTCGCGGCTAA
  1505	TTTGTATGAGTCGCTCCGAA
  1506	TTCCGATCAGTCGATGCAAA
  1507	GATCGTCAATCTGATGCACC
  1508	AGATCGCTAAATGAGGACCC
  1509	GATGCTATAATCGTATGGCC
  1510	AGGAGCGTAAATTATCAGCC
  1511	GGGCGATGACTATATCTGAA
  1512	CTGGATTGACACTAGCATAC
  1513	CTGCGGATACCATAGACAAC
  1514	ACTGCAATAACATATCCGCG
  1515	AATGACATAAAGTGCTGCCC
  1516	ACATGCAGAAAGTAGTCCGC
  1517	ACAGGCGAACAATGTACCCG
  1518	ACCAGCACAAAGTCTACTGT
  1519	AGAGAGCCAAATGACTGTCC
  1520	TAGTGCATAATTGCTTGCCC
  1521	TGAGCATATAGTATTCGGGC
  1522	TGAGCGTTAGAGCTTGATCC
  1523	TAGGCGCTAGGACTCGTTAT
  1524	TATGGCCGACGATGTGTCAC
  1525	TATGGCTGACGTAGCGCACT
  1526	TCTCGGTTACTGAGTGGACT
  1527	ATAACGGGACAGAAGCTGCT
  1528	ATAGAACTCAATAGCCGCTC
  1529	CATAATACACATACGCTGCG
  1530	CAGTACGCAAGCAGATAGCC
  1531	CAGACGCGAAGATAAGTTCC
  1532	CAGCCAAGATAGCATACTCG
  1533	TCCCATAGATAGCTCGCTGG
  1534	TTCGCATGAGTGCTGAGTAC
  1535	TTCCATATACTGGTCGGCAG
  1536	TTTATGATATGCGTCGCGGA
  1537	TTTCTTATATGCGCGAGCGG
  1538	TGTTGCATATTAGCGGCTCG
  1539	TATATGACATCTCTTGCCCG
  1540	TTGTCACATTTGCGCTCCGA
  1541	GCATCCGAATTGCGACGACT
  1542	GGATCTGAATTGCGCGACCA
  1543	GGCTATGAATTTCGCATCAC
  1544	GGATATGCAATTTGTAGCCC
  1545	CAGCGTATAGCAAGATGGAT
  1546	CGAGCGATAATCAAGTCGAG
  1547	CGCGGATGACACATACTCAG
  1548	CGACGAGCACCAATTCGAGA
  1549	CCGTAGTGACCAATGCAGAC
  1550	GCGATATACATCATTCGGAC
  1551	GACAGTCTAATCACTCGTAC
  1552	GCAGTTATACTAAGGTGTGC
  1553	GCAGTAGTAATGAGTGTCAC
  1554	GCAATGTAGTCGAAGTGTCT
  1555	GCATATAGATACCATTCGCG
  1556	CGAATACTAGACACATTGCG
  1557	CAACTACAGTACACAGCGTG
  1558	AGACACAGAACTACCGCGTG
  1559	ATAGCACAACGTAGACGCCG
  1560	ATACAGTCAACTACATCGCG
  1561	AGTACAACCTAGAATCCGGC
  1562	GAAGACTACTAGATACGCGC
  1563	CGATAATACTACAGACTCCG
  1564	CCGTGCGTACACATAGATCA
  1565	CGTGAGCGACACATGATCCT
  1566	CTGTAGTGACATATAGAGCG
  1567	ATGTCGTCACACAGAATACG
  1568	ATGCTACGAACTACCAATCG
  1569	ATGATAACGTACACACCTGC
  1570	TCGGTCTACGTCTGCTCAGT
  1571	GGCTCACGATCCACTGGTTA
  1572	TGCCTGATACCTTGGATGAC
  1573	GGCCGTGAATTATCATAGAC
  1574	GGCTTGGACGCATTGATAAC
  1575	CCCATCGAAGCATGTGTAAA
  1576	CGGCATCGAAGGCGTTCATA
  1577	GCCAGTTGACCACTTCTGAG
  1578	TCGCATTAGCCATGTGGAGC
  1579	GCAATCTAGTCTAATGGCGC
  1580	CTAAGATGTTCTAATCGCCC
  1581	CCAATAGTAAGTAATGGGCC
  1582	TCATTATACTCTGATGGCCC
  1583	ATGCTAATAACTGATCGCCC
  1584	AGTGTCAACCATGATGAACC
  1585	AGAGCATAACATCATGGCCC
  1586	AGAATCTAACAGCGATGCCG
  1587	ATTTAGACAAGTCGATGGCC
  1588	ATATTAAGAAGTAGGCGGCC
  1589	CATATCAGAATACGATGGCC
  1590	GATATACAGGATTATGGCGC
  1591	CATAAATTGGTTCACACCGC
  1592	GAAACTCCAATTCAGCGGAC
  1593	GAACAATGAATTTAGCGGCC
  1594	TTCCATTAGATGTGATGCCC
  1595	TATCATATCATCTGAGGCCC
  1596	ATCAGAAGAACTGCACGTCC
  1597	AGCACAAGAACTACGCGCTG
  1598	AGCAAAGAACCATGCCGCGT
  1599	TAAAGAGCAATGTGGCGTAC
  1600	TTCAGGGCATTGAGCGTAAA
  1601	TTAATGGGCTTGAGCGTATC
  1602	TTAATGCGGTTGAGATCGAC
  1603	GCAGGGATAGCAGATACATC
  1604	TCAGGAGAGGCATCGCATCA
  1605	TTATCTTAGGGATGCGGATC
  1606	TGTGCTCTAGGTCATCCGAG
  1607	TTGTATCTAGTGCGAGGCAA
  1608	TATTATCTAGTATGCGCGGC
  1609	TAGTTATCAGAGTGACTGCG
  1610	GTTAGATCATAGTCACCGCG
  1611	GTTAGTATAGATTGGCCGAC
  1612	GTGTTTATACGTTGAGCACG
  1613	TTATCTGTAGTCATCGAGGC
  1614	TGATACTGAGTTAGCGAGCT
  1615	GTGATCTCAGAGCGCAGCTT
  1616	CAGATGTCAAGACGCGGACT
  1617	CTGGTCAGACAGCGGAATCT
  1618	CGTGGCAGACAGCTAGATAT
  1619	GTGCCGAGACTCCACTGTTA
  1620	GCGGACAGCTCTCCTAGTAT
  1621	ATGCACAACTATCAAGCCTG
  1622	GTGCTTTACTAGCGGAGCCA
  1623	TAAATATCGTATAGGCGGCG
  1624	TAATTCTACTATACGCGGGC
  1625	TAAATCGTATGTAGCAGCGC
  1626	TCCTTCACTGTAGGCTAGGC
  1627	TCAGTTATATGAGCCGACTC
  1628	TCACGTATATTGACTCCGAC
  1629	TCACCGTATTCGAGGCGACA
  1630	TCGTACTGATTGACGGTGAT
  1631	TCACAGCGGTCGAGGTTACT
  1632	TTCACGCGGTCGCAGTATCT
  1633	TACTTGACGTGACTGCATCG
  1634	CGTCACAGAGGACAGCATAC
  1635	TCACTAGAGCGTCGAGCTGT
  1636	TCTACAGTGTGTCAGAGTGA
  1637	CTACCTAATCGACAGCAGAG
  1638	CACCGATAACTACAGCAGGG
  1639	CAACGTCTAGGACAAGGCAG
  1640	CACTAGCTCAGACAGACGAG
  1641	GACTTTACAGTACGATCAGC
  1642	GACACTGACTGACATCGAGA
  1643	GAGACAGTCGAGCGATCAAT
  1644	GCACTTGTACGTCCAGTCAG
  1645	GTACACGGACTGCCAGCATA
  1646	GTAATACGCTATCAGCAGAC
  1647	CTAGATAGACATCACTCACG
  1648	TAGACTCTCGATCAGCCGTA
  1649	GACTTGCACGTACAGCCGAA
  1650	CTTATGCGACACTAGCTCGA
  1651	CTGATGCTACACTAGGCACA
  1652	GCAGACGCACTATCATATAC
  1653	GCAGTAGACACTTCTCACGA
  1654	GCAGGTACACTGACCGACTA
  1655	GCACATCACTGCACGATAGA
  1656	GCAATGACTTCGACTCCAGA
  1657	GACAAGTCATTTACAGGCGA
  1658	GTAACTTGTTTGACAGTGCG
  1659	GACACTGCATGGACAGCGTA
  1660	GCAAGGACTGAGACATGCTT
  1661	TGCGAGGTAGGTTATATCTC
  1662	TGCGGAGAGTGATATACTTC
  1663	GGCGTGAGAGCATTATATCT
  1664	GTGCTGCGAGAGTATTATCT
  1665	CCGCGTGTACCATATAATAC
  1666	GAGCGTGGACGATATACACT
  1667	GGCCGTGTACGATTATGACT
  1668	GTAGCTTGACGATGCTGACT
  1669	GTGCTGGTACTAGCTGCTCT
  1670	TAATGTGACGTAGCCGACTC
  1671	TACCGAGTGCGAGATGCTCA
  1672	TACCGATGTCGATAGATCCA
  1673	TCTCGTATAGGATGAGCAAC
  1674	TCGTGAGTAGGATGCTTTCA
  1675	TACGTGAGATGATGATCGCT
  1676	TAGTCGGTAGCATGAGTCTA
  1677	TAGTTCGAGGAGTAGTCATC
  1678	TAGGTACAGTGCTGGATACT
  1679	CTGCGTCAAGTGTGTAGAAT
  1680	TGTGCGCTAGAGTCTGTCCT
  1681	GGTGCGTCACGATCTCCTAT
  1682	GTGTGGGTACTATGCCATCA
  1683	GCTGATGTACTATCCATACC
  1684	GCTAGATGACGATCAGGTAC
  1685	GCATCTGTACGATCTCAGCA
  1686	GCATCACGACGATTATCAGA
  1687	GCTACGTTACCATGTGCAGA
  1688	GCGTAGTTACCATGCTCACA
  1689	GCGTGAGCACACTCTATCAG
  1690	GCGTGCGAATTATGTATCAG
  1691	TGTGGACACTTCTTATAGGC
  1692	GCGTGAGTAATTTGACTACG
  1693	AGGTGCGTACAAATGCTATG
  1694	CGCAGCCGAAGTACGCTATA
  1695	CGACTGCTAAGGAGCGTACA
  1696	CGATGTTGACAGACCGCACT
  1697	CATGTAGAACTGACTCACAC
  1698	CGAGCGGTAAGGATCTCACA
  1699	ACACGCTGAAAGAGTACGCC
  1700	GATCTGACAGGTAGCGATAC
  1701	TCTCGTGCAGGTAGCTGTCA
  1702	GCTCGGACAGATCGGTATCA
  1703	GCCGGTATAGCTCGATATGC
  1704	GCTGATACAGTTCGATAGAC
  1705	CCTGACTAAGCTCGATAGAG
  1706	GCTGATTACGATCTAGTAGC
  1707	GAATGCTCACGACGAGTAGC
  1708	GAACTGTCCTGACGAATGAG
  1709	TTACTGTCTATGCGATCCGA
  1710	GTTATGTCATCGCAGATTCC
  1711	AGCTATATCAAGCAAGCGTC
  1712	GCTTATACAGTGCAGTAGAG
  1713	TTAAGTAGGTAGCTGGCCTC
  1714	CAAGAGTAACTGCAAGGCCC
  1715	CACTAAGACATGCACAGCGG
  1716	CCTAGTGCAGACCACATGAT
  1717	TCATGCACGTCGCCATAGGT
  1718	TCTATACGCTCGTGCAAGGA
  1719	TCAAGCCCGAGCCGAGTTTA
  1720	TCAGCGCCAGCATTCATGGT
  1721	CCATGCGGACCAAGTCGATA
  1722	GAATGCCGAGCAATGATCCT
  1723	GAATCGGCAGCAATACTGTC
  1724	GAAGCCCAGCTAAGTGGTAT
  1725	AACAGCCCAAACCGGATGGT
  1726	TAAGCACCTTGCAGGATAGA
  1727	TCAGCCCGATCCAGGGTATT
  1728	TATGCGCCCAGGAGGCTTTA
  1729	TGCCCAGCAGGTCGGATTAT
  1730	TAGCTCGCATCACTGACGGA
  1731	GGTCCCATACGAGTGGCATA
  1732	ACTAACCCAACAGCGGAGGT
  1733	CAGCTCTAAGCAGCACAGGA
  1734	CAGGTCAAGCACATACCAGT
  1735	CTGTGCAATCACGCCAGAGA
  1736	CGGCGCAATAATGTCACAGA
  1737	CGGGACATAATTGACACAGT
  1738	AGGGCCAGACAATACACCGT
  1739	GAGGTCACAATTTGCTACAC
  1740	CAGGCACAAGATTGAGCACG
  1741	ACAAGCGCAAATACTGCCGG
  1742	ACAATCTGAAATAGCGCGGC
  1743	ATCGACCCAAGAATAGCTCG
  1744	ATAAGCACAAGCAGCGCGGT
  1745	AACACTCCAAACCGAGGGTG
  1746	AATCTATCAAAGCGACGGCC
  1747	ATTCCCATAACGCGGAGGAC
  1748	ATGCCAGCAACGCGCTAGAA
  1749	ATGCTCACAAGCCACGAGAG
  1750	ATGCTCCAACGATACATACG
  1751	CAGCTTCAAGAGTACATACG
  1752	CATGTCACAAGGGCATAGAC
  1753	CATGGTCTAAGCCCTACAGA
  1754	ACATGGCGAAAGCACCACGT
  1755	CTTAGTTCAATGCACGCACG
  1756	CGCCAGTTAATGCACGACAG
  1757	CAGCAGCAACTCGACTAGAG
  1758	CCGAAGTCAACTGCGCTAGA
  1759	CCAGTGTCAATAAGAGACGT
  1760	CCAGGCGAACTGATCGTAAA
  1761	CCTGGTACAATCAGTAGCAA
  1762	CTAGTGGCAATCATCAGACA
  1763	CAATGCGAACTCACTAGACG
  1764	CATGGCGTACCAATACCTAG
  1765	AAGTGGCCCAAATAACTGCC
  1766	CAAGGCCCAATACACAGGGT
  1767	GATCTGCCAATGCCGCGATA
  1768	GATTCGCCAATGTGCGCTAA
  1769	GAGCCGCCAATGTCACTAGA
  1770	GCGCCCGGAATGTCGTATAT
  1771	GCCGCGCCAATGTTACGTTA
  1772	CTTCGCCCAATGCGTAGGAA
  1773	TTCCCATGATCGCTGACGAG
  1774	TTGCGGGAGCTGCCTCTTAA
  1775	TTTCCCGGATAGCCGCTGTA
  1776	TTTGCTGGAGTATGCGCTCA
  1777	TTGTTCTCAGCTTGCGGCAG
  1778	TGTGTGGCAGCTTAGTTCAC
  1779	TCTTGGGTAGCATCTGTCAC
  1780	TGGGTGTCAGCATCTACGCA
  1781	TTGTGGCAGGTATGCTCCAA
  1782	GTTGGGCACGGATCTCTATA
  1783	GCCGAGGCACCATGCTTATA
  1784	CGCTTGGGACAATCGCGTAT
  1785	CCGCAGGGAACTTCAGCATA
  1786	TGGAGGGCAGTCTCTCATAA
  1787	CTGGGTGCAAGTTGTATCAA
  1788	TGGCGCACATGGTGTCATAA
  1789	TGGCATCACTGCTGCGGAAT
  1790	TGCCAGTCATCCTAGCGTGT
  1791	TCAGGCCAGGACTGCTTATC
  1792	TTGGCATAGGAGTGCTTCTA
  1793	TTTGCAGACGGTGTGCTATA
  1794	TTGAGTCAGGGTGCCCAACT
  1795	TTTAATATCGTTGCCCGAGC
  1796	TCAGGATGATGAGCATGTAC
  1797	CTCAAGCTGGGAGAACAGTA
  1798	TCAGAAGTGGCTGGATCATA
  1799	TCTCACATGGCTGGAGCATT
  1800	CTACTGACACTGACCAGGGA
  1801	TCGTAGCGACTCTCCAGGTT
  1802	TACGTGTCACTATCGTCGAG
  1803	TATAGTTACGTCTCGCACGC
  1804	TACCGTTACGTCGCTCAGAG
  1805	CACTACAACGTGCTACAGAG
  1806	ATAGGTATAACGCAGTACGC
  1807	ATAGCAGTAACGCATAGTCC
  1808	ATAATCGTAACGCACCGACG
  1809	ATGAGTGTAACGCCTCGACA
  1810	ATGTAGCGAACGTACTCACA
  1811	ATCTAGCGAACGGAACTATC
  1812	GTAGAGTCACGATGCAGTAC
  1813	GTAGTATGACGTAGCAGTAC
  1814	GTACGTCGAGCTAGATCGCT
  1815	GAGTCTGTACGAGGTATCAT
  1816	CGTGTCTTACAGCACTACAT
  1817	CGTGCGCTACAGCAGTCATT
  1818	GTAGCCTAGACGCAGTCGTA
  1819	CGTCTCGCAAGTCGCGTATA
  1820	AGTCGCGCACAGCAACGTAT
  1821	ATCGAGGTAACGCCATATAC
  1822	CTCGTGACATAGCCATAGAT
  1823	ATGCGACGAACGCGGATATA
  1824	CTAGACAGACTGCGACATAC
  1825	TAGTCGTAGAGGCGCTATCA
  1826	CTATCGAAGTCGCGTGAAAC
  1827	CTGCGTATAGAGATCAATCC
  1828	CCGCGTATAGACAGATATGA
  1829	CTCGCTTACGACAGACTGGA
  1830	CGCGGAGGAGACATAGCTTA
  1831	AGCGTCACACACAAGACTGG
  1832	CCTACGAGACACATGACAGG
  1833	CGCCGAGTACACATGCAGAT
  1834	CCGTCGATACAGACTCAGAT
  1835	CTCGTCAGACAGAGCGGATT
  1836	GTCTCGCCACGTATCGGATT
  1837	TCTCGCGTACTTAGGCATCA
  1838	GTCTCGGTACGATGTAGCAA
  1839	CGTGTGAGACAGTAGCATAT
  1840	CGTGTAGCACAGCGACGATT
  1841	GTGTAGCTCAGTCAGCATCA
  1842	AGGTAGATAACGCTAGATCC
  1843	CTGTAGAGACATCTGAATCC
  1844	CTGATACGAAGTCTTATGCC
  1845	CACGCTCGAAGACTAATGAC
  1846	CACGCGATAAGACGTATAGC
  1847	CTAGCAGTAAGTCTATGCAC
  1848	CGTAGTTGAAGTCATCGACA
  1849	CGCGATAGAAGTCAGGACAT
  1850	GACGGACGACATCTGAGCAT
  1851	CATAGACGAATACAGCGGGC
  1852	GATCACGACCTACTAGCAGG
  1853	AGATATAACGAACTCTCGCG
  1854	GATTATAGACTACTGAGGCC
  1855	GAGTTTATACTACAGTGCCG
  1856	GTCACTTACGCTCAGGCAGA
  1857	TCGCTAGACGCTCTGGCATA
  1858	GTACGCTCAGCACTGGCATT
  1859	GACGCGCTAATACTGTCACA
  1860	GCGTGCATACGACTGCCATA
  1861	TGTAGTCTAGTGCATGGTCA
  1862	GTATAGTCAGAGCTGGCACC
  1863	CGTCAGTCAAGTATGGCACA
  1864	ACGAGAGTAAATATGCTGCC
  1865	ATAGAGCGAACGATAGTTGC
  1866	ATCTGACTAACGATGATGCC
  1867	GTTGTAGGACGTATGATCTC
  1868	TTAGTCGAGTCTATGAGCCC
  1869	CGACGATACAGTAATCTAGC
  1870	CTGATACAGGCATAGACATC
  1871	GGTATCAGAGCTAGGACTAT
  1872	TCTATCTCAGCTACGGTCGA
  1873	TCAGTTCGATCTACGGCTAG
  1874	TCAGTGCGACTCAGGTACGA
  1875	GTCACTGCACTCACGGTAGA
  1876	TAACGAGTCTTCAGCACGTA
  1877	GAAGTCGCCTACATAGCCTA
  1878	GAAGTCCGTTACATGACCAT
  1879	GTCAGAGGATCGAGCCACTT
  1880	GCGAGACAGGTCAGTACAAT
  1881	CGTCAGAAGGCTCGCACATA
  1882	GCATACAGGTTACGACGCCT
  1883	GCGATACAGGTTCAGAGATA
  1884	GGACGCATAGCTCGCAGTAT
  1885	GGACGCAGATCGCAGCATAT
  1886	CGGCGTTAATCGCAGAGAAC
  1887	CGCGTTCTAAGGCACGGATA
  1888	CGCGTCGCAAGGCTGTTATA
  1889	CGATACGCAAGGCTACGACA
  1890	CATCTAAGGACACTACACTG
  1891	TATCATCGAGGACTCAGTGC
  1892	CACCGAGCAAGACTGACATG
  1893	CGCACCCGAAGTCAGAGATA
  1894	CGGCTAGGAAGTCAGCATAA
  1895	ATGCTGCGAACGCGCCATAA
  1896	CCGCGTGCAACGTGTTCATA
  1897	GTCGCTGCATAGCATCTCAG
  1898	GTCTGTGCATAGAGCGTCAT
  1899	GTGGTGTCACTGATACGTCA
  1900	GGTTAGCACTAGATCGCACT
  1901	CGGGATCTACAGCATCATAG
  1902	CTGGATATACAGCACTCACA
  1903	ATGCGGCTAACGCCTCATAA
  1904	TCGCGGCGCACTCTGTTATA
  1905	TCGTGCTACTGCCACTGTAT
  1906	TAGGACACTTCGCCACTATG
  1907	TATGACAGTTCGCGCTACCG
  1908	TCGCGCAGTTAGCCCTATGT
  1909	TAGCCACCGTAGCTGATCGT
  1910	GTAACCCGCTATCAGATCGA
  1911	AGAGCGCAACACCACATTGT
  1912	AGGCTAAGAACGCACACTCG
  1913	GAGCCTAGACAGCTTCATAC
  1914	GGCAGTTCACGACTCGACAT
  1915	GGCCTTAGACGACTCGCATA
  1916	GGTCGATCAGCACTGCATAC
  1917	GGAGAGTCAGCACAGTCCTA
  1918	GTATAGGCAGCACGGCTCAT
  1919	GCACGGCGAGCACTATCTTA
  1920	TAACGTCCTGCACGATCTGT
  1921	GGACGCCTAGCACATCTGAT
  1922	CGCTGCACATCACATGGATT
  1923	GCACATCGAGCACATGCAGT
  1924	GCACGACCAGCTCTTAGGAT
  1925	CCCACCAGACAGATAGAGGT
  1926	CCCGACGCACGAATAGATAG
  1927	CCCACGACAGATACATGAGT
  1928	CTTCGCGCAGCTACATAGAT
  1929	CGCTCCGAAGCTGCGATAAT
  1930	CGCCGCGTAAGCAACAAATT
  1931	CGACGCTCAAGGACTCATAA
  1932	CGCACACTAAGGATCATTAC
  1933	AGACACGCAAGAAGCTGGCT
  1934	GCACGCATAGCAGAGGATCT
  1935	GCTACGTCACTGAGCAGGAT
  1936	GTACATCTCGTGAGCAGAGC
  1937	CTACACGACTTGAGACGAAG
  1938	CTAAGTACGTGCAAGCAAGG
  1939	GACACGTAGGACAGCTATGC
  1940	GACATAGTAGACATCTCACG
  1941	GACAGCGTAGACATCGTCAG
  1942	GACTATCACGACATTCAGCG
  1943	GATCTACACGCTACCAGTGG
  1944	GCTTACTACGGATAGATCAG
  1945	GCGTATCTAATGGAGTAGCA
  1946	GCGTATTTACAGTGAGCGAC
  1947	GCGTATATCGAATTGAGTGC
  1948	GCGTTCACAGAGTCCACGAT
  1949	CGCGTATCAAGGTCACGACA
  1950	GCTATTACAGTGTCAGAGAC
  1951	CGTCAGATAAGGTGAGTTAC
  1952	CGTCTGTGAAGGTCAGCTAA
  1953	TATTAGCACTCGTCAGCAGC
  1954	ATGTTATCAACGTCAGCGAC
  1955	GGCATACTAGAGTCAGCGAT
  1956	AGTGCGATACAATACGAGCG
  1957	CAGCACACAGAGTACAGCGT
  1958	CGTAGCATAAGGTCAGCACC
  1959	GTCCATAGACGTTGATACCA
  1960	GCTACGATAGATGAGCCACG
  1961	CGGAGTACACCAGATCCAGA
  1962	GAGCGTATAGGAGATCCAAC
  1963	GACTGTAGAGAGACGATCCA
  1964	CTAGTAGGAAGTGCGATCAA
  1965	CGTAGAGGAAGTGATACTCA
  1966	CGTATCGGAAGTGAGTATCA
  1967	CTATGACGAAGTGAGAGTAC
  1968	GTTCGTAGAGATGATCGTCA
  1969	GTTCTCAGATAGTATGCAGC
  1970	AGTCTGTTAAGATATGCGCC
  1971	AGCACGGAACAGTAAGCCCT
  1972	ATCCAGAGAACGTGAGATCC
  1973	GACAGTGTAATATGAGGACC
  1974	CATAGTAGAAGATTCGAGCC
  1975	TGAGATATAGTATGCGGCCA
  1976	ATGAACATACTATACCGCGC
  1977	TTCTCTATATCGTGCGCGGA
  1978	TGAGTTTACGTGTATGGCAC
  1979	ACGGCATCAAAGTTGCATAC
  1980	ACGGGCTCAAAGTATGATAG
  1981	AGGCGCTTAAATGTGGATAC
  1982	CTGCCGTTAATGGCGGACAT
  1983	CTGAGCCAATAGGCGCACTT
  1984	TAGGCATGATGAGAGCTATC
  1985	TGCCTATGAGGAGTATGAAC
  1986	GGGCTATAATGAGCTTGACT
  1987	TAGGCTTCATCAGCTATCAG
  1988	ATTGCTTCAACGGGCATTAC
  1989	TATGATCCATGCGACTCGGA
  1990	TTGTATCCATCGGCCCAGTG
  1991	ATCAAGGCAACCGCCAGTAG
  1992	TCTCAGCCATCCGTGATAGG
  1993	TATCAGGCATCCGAGCATAG
  1994	TTAAGCTCCTCAGTCCATGT
  1995	TAAGGGCGATGAGCCTATCT
  1996	TAAGGCCGAGGAGCTTTCAT
  1997	TAAGGCAGTGGAGCCCTCTA
  1998	TGGACAGGCTGCGCTCTATA
  1999	CTGGAAGCCTGCGACCAAAT
  2000	TCAATGCACTGAGCCCGAGA
  2001	GATTCACACTGACCCATGTA
  2002	TAAATAGATTGGAGACGCGC
  2003	GCATTAGAAGGTCTGGACTA
  2004	ATTGGCATAACGTATTGCGC
  2005	CAGGACTGAAGATCGAGTAC
  2006	TAGAGTCAGTCATAGCTCGA
  2007	TTTATCGTAGCTGGCTGCCC
  2008	AGGATTAGAACCTACGCACC
  2009	GCCGTGAGACCACTGTACTA
  2010	GACGCTGAATCCTATTGACA
  2011	CGCCTAAGGATCGTGAAGTA
  2012	CGACGACGAAGCTGCATGAA
  2013	ACTCGAATAACAGCATCTCG
  2014	CCCGTAAGCATGGCACAGAT
  2015	CAATACAAGATTACGGCCTC
  2016	GATCAGAATCTATGGTACGC
  2017	TCTGTGTACTGCTCGCCAAT
  2018	ATATTTGGAACGCAGCTCAC
  2019	TGCAGTATCGCAGCGGTTCTA
  2020	GGGCAATGTTTATCCACAGA
  2021	CTGACCGAATCCAGCAGAGA
  2022	GATCGTGAATCCGCGCACTA
  2023	GAGCCGTAATCCGAGCGATA
  2024	TACTCCTGACGACTTAGGCA
  2025	TGCTGTCACTCGGCGTCTAT
  2026	GTACTAGCATATCATCGACG
  2027	TATCGCATAGATCAGTGAGC
  2028	TACGGGCAGCCAGGTACTTT
  2029	GTTCATCACGAGTGCGTAGA
  2030	CATGTATCAAGATGGCTGAC
  2031	GGTCGCGCATTCCAGCATA
  2032	GCACATATCTAGCGACATCT
  2033	ACGCGGCTAAAGGTAGATAC
  2034	CACTGCCCACAAGATGTAGA
  2035	GGATTTACATGGCCTAGCAA
  2036	CATGACACAGAATCGACCGT
  2037	AGAGGCATAAATGAGTCTCC
  2038	TGAGTAGTACGTTACGCCTG
  2039	CGATAGCGAAGGAGTCCACA
  2040	ACACTCTGAAAGACGCGACG
  2041	GTCTTAATGTTGGGCAACG
  2042	GTTATCGACTACGCTGTACT
  2043	TCGTGAGACCGTCGTCAGTA
  2044	GACAGCGCAGTACAGGTAAT
  2045	CGTACAGTAAGTATGATGCC
  2046	TAGAGCATCTGACGCTATGA
  2047	GTCACGATTAGTAGGCACG
  2048	TCGTACCTGTATTCAGCGCG
  2049	TTAATCCGCTGTAGCCCAAA
  2050	TTAATTGACTTCGCTCCAGC

EXPERIMENTS
• Arrays containing probes corresponding to SEQ ID NOS 1-2050 were designed and manufactured using known photolithography techniques. Four probes were designed to interrogate each sequence from SEQ ID NOS 1-2050: a probe designed to be the perfect match complement to the sequence (PM), a probe designed to have a central mismatch at position 10 (MM), and probes designed to be the complements to the PM and MM probes (cPM and cMM respectively).
• FIG. 2 shows an example of the sequences attached to each of the four array features representing a given tag sequence. The first block contains the cPM probe. The second block contains the cMM probe. The third block contains the PM probe—the probe to which the tag is expected to hybridize with the highest affinity. The fourth block contains the MM probe.
• FIG. 3 shows the array features from the above-described array. The array was hybridized with biotin-labeled oligonucleotide tags, stained with streptavidin-phycoerythrin, and the data was collected with a laser scanner. Four features, organized vertically on the probe array, represent each tag-probe. For each of the four tag-probes shown, arranged horizontally across the array, the brightest hybridization signal is seen with the “PM” feature.
• FIG. 4 is a scanned image of the hybridization pattern resulting from the hybridization of 2050 different oligonucleotide tags labeled with phycoerythrin to an array designed as described above.
• FIG. 5 is a scanned image of the hybridization pattern resulting from the hybridization of 50 sequences complementary to SEQ ID Nos. 2001-2050 to an array designed as described above.
• FIG. 6 shows signal intensities from two different independent experiments in which 2000 biotinylated oligonucleotide tags or 50 fluorescein labeled control oligonucleotides were hybridized to arrays designed as described above. The frequency of results are shown as normalized (to scale of 0-1, in bins of 0.05) natural logarithms of the net signal intensities. The normalized natural logarithm of the signal intensities obtained are distributed about a geometric mean of 0.8 with a standard deviation of less than 0.1.
• FIG. 7 shows the PM/MM ratios from the data described inFIG. 4 above. More than 98% of the hybridization's yielded a PM/MM ratio greater than 3/1.
CONCLUSION
• The above descriptions are illustrative and not restrictive. Many variations of the invention will become apparent to those of skill in the art upon review of this disclosure. The scope of the invention should, therefore, be determined not with reference to the above description, but instead should be determined with reference to the appended claims along with their full scope of equivalents.

Claims (3)

1-12. (canceled)

13. A method of analyzing a nucleic acid sequence comprising:

attaching said nucleic acid sequence to a nucleic acid tag to form a sequence-tag complex wherein said nucleic acid tag is a sequence chosen from the group consisting of:

SEQ ID NOS: 1-2000;

the complements of SEQ ID NOS: 1-2000; and

hybridizing said sequence-tag complex to the complement of said nucleic acid tag.

14. The method ofclaim 13 wherein said complement of said nucleic acid tag is attached to a solid support. _

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