US20060136047A1 - Tissue graft prosthesis devices containing juvenile or small diameter submucosa - Google Patents

Abstract

Described are preferred tissue graft materials that incorporate juvenile submucosa tissue from a warm-blooded vertebrate. Preferred materials incorporate juvenile small intestinal submucosa tissue from a mammal such as a porcine mammal, and the constructs are preferably in tubular form and utilized the isolated submucosa tissue in its native, intact tubular form. More preferred devices are multi-laminate and include the juvenile submucosa tissue in addition to at least one, and preferably several other layers providing increased strength or other advantageous properties to the construct.

Description

SUMMARY OF THE INVENTION
• Accordingly, one aspect of the present invention provides a tubular graft prosthesis that includes a tubular element having walls and a lumen. The walls of the prosthesis include juvenile submucosa tissue from a warm-blooded vertebrate. In preferred forms, the juvenile submucosa tissue retains a natural, intact tubular form, especially having a diameter not exceeding 12 mm. The juvenile submucosa can be positioned in the prosthesis so as to provide the innermost layer, and thereby define the surface of the lumen. More preferred prosthesis devices of the invention will include at least one additional wall layer which can, for example, be another layer of collagenous tissue such as juvenile or adult submucosa tissue. One or more layers of a synthetic material may also be provided.
• In another embodiment, the invention provides a tissue graft composition that in includes juvenile submucosa tissue of a warm-blooded vertebrate.
• In another embodiment, the invention provides a small-diameter tubular graft construct that includes a tubular element having walls defining a lumen. The walls include at least a first layer formed with an intact tubular submucosa segment having a native internal diameter no greater than about 12 mm. The walls in such constructs may also include one or more additional layers formed with tissue materials and/or synthetic polymer materials as described further hereinbelow.
• In still another aspect, the present invention provides a method for tissue grafting in an animal that comprises grafting the animal with a tissue graft material including juvenile submucosa tissue from a warm-blooded vertebrate.
• The present invention provides improved and alternative tissue graft prosthesis devices including tubular graft constructs, and manufacturing and grafting methods involving the same. Additional embodiments and features and advantages of the invention will be apparent from the descriptions herein.
DESCRIPTION OF THE DRAWINGS
• FIG. 1 provides a perspective view of a tubular graft prosthesis device of the present invention.
• FIG. 2 provides a cross-sectional view of the tubular graft prosthesis device depicted inFIG. 1 taken along line2-2 and viewed in the direction of the arrows.
• FIGS. 3A-3G depict steps used in the manufacture of a 5-layer tubular prosthesis device of the invention.
• FIG. 4 depicts a tubular submucosa covered stent device in accordance with the invention.
DETAILED DESCRIPTION
• For the purpose of promoting an understanding of the principles of the invention, reference will now be made to certain embodiments thereof and specific language will be used to describe the same. It will nevertheless be understood that no limitation of the scope of the invention is thereby intended, such alterations, further modifications and applications of the principles of the invention as described herein being contemplated as would normally occur to one skilled in the art to which the invention relates.
• As disclosed above, one aspect of the present invention provides tissue graft prosthesis devices that incorporate juvenile submucosa tissue from a warm-blooded vertebrate. Preferred graft devices include a tubular element having walls and lumen, wherein the walls include juvenile submucosa tissue or small-diameter tubular submucosa tissue from a warm-blooded vertebrate. In this regard, as used herein, the term “juvenile” refers to a warm-blooded vertebrate having an age of not greater than about 30 days. This includes both fetal (prenatal) submucosa tissues and those taken from postnatal animals. The term “small diameter” as used herein refers to tubular materials having an internal diameter no greater than about 12 mm. For example, small diameter intestinal submucosa may be obtained from juvenile animals, and/or from older (including adult) animals of dwarf, pigmy, or other unusually small breeds. As well, the animal from which the submucosa tissue is taken may be male or female.
• With reference now toFIG. 1, shown is a perspective view of atubular graft prosthesis10 in accordance with the present invention.Tubular graft prosthesis10 defines aninner lumen11 and has a length L and diameter D rendering the construct suitable for the intended use, for example a vascular use.
• With reference now toFIGS. 1 and 2 together, shown inFIG. 2 is a cross-sectional view of theprosthesis10 ofFIG. 1 taken along line2-2 and viewed in the direction of the arrows.Prosthesis10 has walls defininginner lumen11, preferably including several layers of material as illustrated. In particular, shown inprosthesis10 is a firsttubular layer12, a second layertubular layer13, a thirdtubular layer14, a fourthtubular layer15, and a fifthtubular layer16. In accordance with certain aspects of the invention, at least one of these layers includes juvenile submucosa from a warm-blooded vertebrate animal, or otherwise includes an intact tubular submucosa segment having a small native internal diameter (12 mm or less). The animal is preferably a mammal, such as a porcine, ovine, bovine, or other mammalian animal. Human donor tissues may also be used in the present invention. In the case of juvenile porcine submucosa, the animal at harvest will typically not exceed about 10 kilograms (kg).
• In preferred aspects of the invention, the juvenile or other small diameter submucosa tissue will retain its intact, tubular form as harvested from the animal. More preferably, at least theinnermost layer12 will be formed from intact, tubular juvenile submucosa tissue. In this fashion, thesurface17 of thelumen11 will be defined by the intact juvenile submucosa tissue, and will be free of any seams that would otherwise be created when configuring sheet-form tissue into a tube. Preferred devices will include at least one additional layer, for example,layers13,14,15 and16 as illustrated inFIG. 2. These additional layers can be made of any suitable material and desirably provide reinforcement and strength to the device supplemental to that provided byinnermost layer12. Wheninnermost layer12 is comprised of juvenile submucosa tissue, one or more oflayers13,14, and15 may, for example, be formed of synthetic materials such as synthetic polymer materials. Suitable synthetic materials may be biodegradable or non-biodegradable materials. These include, for example, synthetic biocompatible polymers such as cellulose acetate, cellulose nitrate, silicone, polyethylene teraphthalate, polyurethane, polyamide, polyester, polyorthoester, polyanhydride, polyether sulfone, polycarbonate, polypropylene, high molecular weight polyethylene, polytetrafluoroethylene, or mixtures or copolymers thereof; polylactic acid, polyglycolic acid or copolymers thereof, a polyanhydride, polycaprolactone, polyhydroxy-butyrate valerate, polyhydroxyalkanoate, or another biodegradable polymer.
• In certain embodiments of the invention wherelayer12 is comprised of juvenile submucosa, one or more of, and potentially all oflayers13,14,15 and/or16 are formed from additional collagenous materials. For example, suitable collagenous materials include extracellular matrix layers including, for instance, submucosa, renal capsule membrane, dura mater, pericardium, serosa, peritoneum or basement membrane layers, including liver basement membrane. These layers may be isolated and used as intact membranes, or reconstituted collagen layers including collagen derived from these materials or other collagenous materials may be used.
• Desirably,layers13,14,15 and16 are made from additional submucosa tissue layers. Suitable submucosa tissues for these purposes include, for instance, intestinal submucosa including small intestinal submucosa, stomach submucosa, urinary bladder submucosa, and uterine submucosa. Small intestinal submucosa, when employed, can be used in an intact, native tubular form or can be a tubular form shaped from flat sheets including one or more seams along all or a portion of its length. Desirably, at least one oflayers13,14,15 and16 will include adult submucosa tissue, as such tissue in its native condition is generally superior in mechanical properties to juvenile submucosa tissue. In this fashion, adult submucosa tissue can be used to provide strength to theoverall graft construct10. Porcine small intestinal submucosa is particularly preferred for these purposes.
• In one form,intermediate layers14 and15 can be made from adult small intestinal submucosa, andintermediate layer12 andoutermost layer16 can be made from juvenile small intestinal submucosa, preferably again in its native, intact tubular form. In this fashion, seamlessinner layer12 and seamlessouter layer16 can be provided.
• Layers12,13,14,15 and16 can be adhered to one another so as to generally form a unitary construct. This adherence may be achieved, for example, by crosslinking, including for example dehydrothermal crosslinking or chemical crosslinking, and/or by the use of a bonding agent. As bonding agents for these purposes, one may use fibron glue, or gelatin or collagenous pastes in sufficient amount to bond adjacent layers to one another.
• Tubular devices of the invention may be prepared, for example, by positioning the appropriate tissue layers over a mandrel, and subsequently bonding or adhering the tissue layers together to form a generally unitary tubular construct. This may be accomplished, for instance, using intact tubes, and/or by wrapping or winding sheet- or strip-form adult submucosa tissue around the mandrel to form overlapped sections which are subsequently bonded or adhered. In some embodiments, an outermost covering layer may be provided by an intact juvenile submucosa segment positioned over the underlying tissue layers. If a bonding agent is to be used in forming the construct, the agent or its components can be applied at appropriate points intermediate the application of layers to the mandrel. The entire construct can then be dried, e.g., lyophilized and/or dried under vacuum, to form the overall tubular graft construct.
• In some embodiments of the invention, tubular prosthesis devices are prepared using a two component bonding agent such as fibrin glue (e.g., having thrombin and fibrinogen as separate components). To prepare such devices, subsequent layers are added after coating the previously-applied layer with a first component of the bonding agent (e.g., thrombin) and coating a layer to be applied with a second component of the bonding agent (e.g., fibrinogen). Thereafter, the layer to be applied is positioned over the previously-applied layer so as to bring the two bonding components into contact, thus causing the curing process to begin. This process can be repeated for any and all additional layers to be applied to the tubular construct.
• With reference now toFIGS. 3A through 3G, an illustrative manufacture of a 5-layer (5L) tubular device of the invention will now be described. An intacttubular submucosa segment21 from a juvenile animal may first be positioned over amandrel20 as depicted inFIG. 3A, to provide a one-layer (1L) construct. Thrombin (light shading,FIG. 3B) is then applied to theintact segment21. A second intact tube ofjuvenile submucosa22 is provided either on an extension of the same mandrel as illustrated, or on a second mandrel connectable to the first mandrel. The secondintact segment22 is coated with fibrinogen (dark shading), and thesegment22 is positioned immediately adjacent thefirst segment21.Segment22 is then and pulled over the first submucosa tube in a fashion causing inversion of the tube22 (FIGS. 3C-3E). Thus, leadingend25 ofsegment22 remains substantially in place, but inverted, in contact with the trailingend23 ofsegment21. Trailingend26 ofsegment22 finally inverts and comes into contact with leadingend24 of thefirst segment21. In this fashion, portions of the two submucosa tubes coming together will remain substantially together, i.e., one, submucosa layer will not pulled along another submucosa layer. This is beneficial in that as the bonding agent begins to cure, movement of submucosa layers relative to one another becomes difficult. The inversion of the second tube is continued until it is completely inverted and lying atop the first submucosa tube, creating a two-layer (2L) construct as illustrated inFIG. 3E. The fibrin glue of the 2L construct is then allowed to cure (typically 1 to 5 minutes). The outer surface of the 2L construct is coated with thrombin (light shading,FIG. 3F). Fibrinogen (dark shading,FIG. 3F) is then applied to one surface of asheet27 of adult submucosa of a dimension sufficient to encircle the prior-applied layers two times. As illustrated inFIGS. 3F-3H, the adult submucosa is then wrapped around the 2L construct for a single turn (clockwise rotation inFIGS. 3F-3G), resulting in a completed three-layer (3L) construct on themandrel20. During or after curing of the applied fibrin glue components, thrombin is applied to the outer surface of the 3L construct (light shading,FIG. 3I). A second turn of theadult submucosa sheet27 is then completed (FIG. 3I), bringing the applied thrombin and fibrinogen components into contact with one another, and forming the completed four-layer device (4L,FIG. 3J). During or after cure of the newly-contacted fibrin glue components, a third intact tubular juvenile submucosa segment28 (FIG. 3K) is positioned adjacent to the applied layers, thrombin (light shading) is coated onto the 4L construct and fibrinogen (dark shading) is coated onto the thirdtubular segment28. The thirdtubular segment28 is then pulled and inverted over onto the applied layers (FIGS. 3K-3M) as before to complete the 5L construct (FIG. 3N), with the leadingend31 of thethird segment28 lying atop the trailingend29 of segment the 4L construct, and the trailingend32 ofsegment28 lying atop the leadingend30 of the 4L construct. The device is then allowed to cure and is trimmed as necessary. It will be understood that the application of thrombin and fibrinogen or any other two-components for the bonding agent could be reversed in order. As well, the components of the bonding agents can be applied by any suitable method, including spray or brush application methods, and intermediate constructs can be hydrated at appropriate points in the manufacture. The entire construct may then be freeze-dried or otherwise processed if desired.
• Submucosa for use in the invention can be derived from any suitable organ or other biological structure, including for example submucosa tissues derived from the alimentary, respiratory, intestinal, urinary or genital tracts of warm-blooded vertebrates. Submucosa useful in the present invention can be obtained by harvesting such tissue sources and delaminating the submucosa from smooth muscle layers, mucosal layers, and/or other layers occurring in the tissue source. For additional information as to submucosa useful in the present invention, and its isolation and treatment, reference can be made to U.S. Pat. Nos. 4,902,508, 5,554,389, 5,993,844, 6,206,931, and 6,099,567.
• As prepared and used, the juvenile submucosa tissue and any other tissue used, may optionally retain growth factors or other bioactive components native to the source tissue. For example, the submucosa or other tissue may include one or more growth factors such as basic fibroblast growth factor (FGF-2), transforming growth factor beta (TGF-beta), epidermal growth factor (EGF), and/or platelet derived growth factor (PDGF). As well, submucosa tissue used in the invention may include other biological materials such as heparin, heparin sulfate, hyaluronic acid, fibronectin and the like. Thus, generally speaking, the submucosa or other tissue may include a bioactive component that induces, directly or indirectly, a cellular response such as a change in cell morphology, proliferation, growth, protein or gene expression. Further, in addition or as an alternative to the inclusion of such native bioactive components, non-native bioactive components such as those synthetically produced by recombinant technology or other methods, may be incorporated into the submucosa tissue.
• Submucosa tissue used in the invention is preferably highly purified, for example, as described in U.S. Pat. No. 6,206,931 to Cook et al. Thus, preferred material will exhibit an endotoxin level of less than about 12 endotoxin units (EU) per gram, more preferably less than about 5 EU per gram, and most preferably less than about 1 EU per gram. As additional preferences, the submucosa material may have a bioburden of less than about 1 colony forming units (CFU) per gram, more preferably less than about 0.5 CFU per gram. Fungus levels are desirably similarly low, for example less than about 1 CFU per gram, more preferably less than about 0.5 CFU per gram. Nucleic acid levels are preferably less than about 5 μg/mg, more preferably less than about 2 μg/mg, and virus levels are preferably less than about 50 plaque forming units (PFU) per gram, more preferably less than about 5 PFU per gram. These and additional properties of submucosa tissue taught in U.S. Pat. No. 6,206,931 may be characteristic of the submucosa tissue used in the present invention.
• A typical layer thickness for the as-isolated-juvenile submucosa layer used in the invention ranges from about 50 to about 200 microns when fully hydrated. This layer thickness may vary with the type and age of the animal used as the tissue source. As well, this layer thickness may vary with the source of the tissue obtained from the animal source. In particular, when juvenile mammalian submucosa tissue is used in the invention, the as-isolated submucosa layer will typically have a thickness in the range of about 80 to about 150 microns when fully hydrated, and the tissue will be generally more compliant than adult tissue from the same species. Other characteristics of juvenile small intestinal submucosa include, for example, a native inner diameter of about 1 mm to about 12 mm, more typically about 3 mm to about 8 mm. The native juvenile submucosa tissue may also exhibit a higher level of solubility in urea or other similar collagen-degrading agents, evidencing a difference in composition likely relating at least in part to the maturity of the collagen. Porcine, ovine, or bovine submucosa tissues having these characteristics are preferred for use in the present invention, particularly porcine small intestinal submucosa.
• Submucosa tissue used in the invention may be free of additional, non-native crosslinking, or may contain additional crosslinking. Such additional crosslinking may be achieved by photo-crosslinking techniques, by chemical crosslinkers, or by protein crosslinking induced by dehydration or other means. Chemical crosslinkers that may be used include for example aldehydes such as glutaraldehydes, diimides such as carbodiimides, e.g., 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, ribose or other sugars, acyl-azide, sulfo-N-hydroxysuccinamide, or polyepoxide compounds, including for example polyglycidyl ethers such as ethyleneglycol diglycidyl ether, available under the trade name DENACOL EX810 from Nagese Chemical Co., Osaka, Japan, and glycerol polyglycerol ether available under the trade name DENACOL EX 313 also from Nagese Chemical Co. Typically, when used, polyglycerol ethers or other polyepoxide compounds will have from 2 to about 10 epoxide groups per molecule.
• When additionally crosslinked, submucosa tissues of the invention can be additionally crosslinked internally within a single layer, and/or crosslinking may be used in whole or in part to bond multiple submucosa layers to one another. Thus, additional crosslinking may be added to individual submucosa layers prior to bonding to one another, during bonding to one another, and/or after bonding to one another.
• Graft constructs in accordance with the invention can be used to graft mammalian patients, including humans. Preferred, tubular graft constructs of the invention find particular utility in repairing or replacing tubular structures within the body. For example, tubular graft constructs of the invention are used with preference in vascular applications nerve tube applications, ductal repair or replacement, urethral repair or replacement, or ureter repair or replacement. Vascular applications include, for example, use as arterial or venous grafts, and/or bypass grafts. Generally, tubular graft constructs of the invention will have internal diameters ranging from about 1 mm to about 30 mm, more typically in the range of about 1 mm to about 12 mm, and most typically in the range of about 3 mm to about 8 mm.
• Graft constructs of the invention may include coatings or other incorporated materials to assist in reducing the frequency or incidence of thrombosis when used in the vasculature. For example, grafts in the invention may be coated with heparin. In this regard, the heparin may be bound to the graft construct by any suitable method including physical, ionic, or covalent bonding. In one preferred embodiment, heparin is bound to the collagen construct using a suitable crosslinking agent such as a polyepoxide as described hereinabove. In multi-layer constructs, heparin or other agents can be applied to the layers individually before incorporation of the layer into the construct, after the layers are incorporated into the construct (e.g. coating a luminal surface of an inner tubular layer), or both.
• Prosthesis devices of the invention may optionally include medical structures other than tissue and/or polymer layers. For example, tissue graft materials including juvenile or other small-diameter submucosa may be attached or otherwise mounted in combination with stents, rings, valves, or other similar medical structures. In such devices, the tissue graft material may for example be used as a coating to facilitate tissue incorporation of the medical structure, and/or may be used to create one or more functional tissue segments, such as tissue valve structures, associated with the medical structure. With reference toFIG. 4, in certain embodiments of the invention, the tissue graft material including juvenile or other small diameter submucosa is used as acoating41 for astent42 comprised of wire or another biocompatible material, to form a coated orsleeved stent device40. In such devices, an intact cylindrical segment of juvenile or other small diameter submucosa provides an effective cylindrical coating orsleeve41 for thestent42. In this regard,cylindrical coating41 may comprise a single layer or multilaminate construct in configurations described hereinabove, or still other configurations, and may optionally be attached to thestent42 at one or more locations along its length, for example by sutures, adhesives, bonding, or other attachment means. Thestent41 may be a self-expanding stent, or an expandable stent (e.g. by balloon), useful for example in vascular, gastrointestinal, or other body passageways. In one mode of construction, thecoating41 may be applied to thestent42 while thestent42 is in its expanded state, and thestent42 thereafter converted to a retracted state, preferably without causing significant damage to thecoating41. Thecoating41 may optionally be in hydrated condition during retraction to facilitate this operation. Thecoated stent40 can thereafter be conventionally processed and packaged for medical use.
• The invention also encompasses medical products including a prosthesis device of the invention sealed within sterile medical packaging. The final, packaged product is provided in a sterile condition. This may be achieved, for example, by gamma, e-beam or other irradiation techniques, ethylene oxide gas, or any other suitable sterilization technique, and the materials and other properties of the medical packaging will be selected accordingly.
• For the purposes of promoting an additional understanding of the invention and its features and advantages, the following specific examples are provided. It will be understood that these examples are illustrative, and not limiting, of the invention.
EXAMPLE 1Isolation of Juvenile Intestinal Submucosa
• Frozen intact juvenile porcine small intestine was immersed in tap (<38° C.) water until it was thawed. At room temperature, the intestine was then cleaned out by running tap water through the entire length to remove any remaining chyme. Then, the intestine was cut into one-foot lengths and hand-scraped with a Teflon plate. Scraped tubular submucosa pieces were placed into high purity water to keep hydrated until the material could be disinfected.
EXAMPLE 2Disinfection of Juvenile Intestinal Submucosa
• Isolated juvenile porcine small intestine submucosa was submerged into one liter of 0.2% (v/v) peracetic acid/0.05% (v/v) ethanol solution and was shaken for two hours at room temperature. After two hours, the peracetic acid solution was drained, and high purity water was added. The submucosa was then shaken at room temperature for five minutes and drained. Subsequently, the juvenile submucosa was rinsed three more times at room temperature with high purity water. Finally, the intact tubular juvenile submucosa was stored in high purity water at 4° C.
EXAMPLE 3Preparation of Multilaminate Tubular Graft Construct
• The luminal juvenile submucosal layer was pretreated with an antithrombogenic heparin coating. Then, the treated mucosal surface was positioned on a stainless steel mandrel so that the mandrel faced the lumen. The outer serosal surface was sprayed with the thrombin component of fibrin glue, while another piece of juvenile submucosa was inverted on another stainless steel mandrel, so that the serosal surface was contacting the mandrel. This piece was sprayed with the fibrinogen component. The ends of the two pieces were secured, and the inverted juvenile submucosa was reinverted onto the first layer. The fibrin glue was allowed to cure for two minutes at room temperature and submerged in high purity water. A sheet of hydrated adult small intestinal submucosa was laid out and completely sprayed with thrombin, and the outer surface of the two-layer graft was sprayed with fibrinogen. Then, the tubular submucosa was carefully laid on the adult submucosa and wrapped once with the adult submucosa. The remainder of the sheet was covered with Parafilm, and the outer surface of the third layer was sprayed with fibrinogen. One complete turn of the mandrel was made, and the excess adult submucosa was trimmed. The fibrin glue was allowed to cure for two minutes at room temperature, and the entire four-layer graft was submerged into high purity water for two minutes. A final layer of juvenile submucosa was inverted onto another stainless steel mandrel and sprayed with thrombin. The four-layer graft was then sprayed with fibrinogen. The final juvenile submucosa layer was reinverted onto the outer surface of the four-layer graft and allowed to cure for two minutes at room temperature. The graft was submerged back into high purity water for thirty minutes, then frozen for three minutes in a −80° C. freezer. Finally, the graft was lyophilized overnight, trimmed, packaged, and sterilized via ethylene oxide sterilization.
EXAMPLE 4Utilization of Multilaminate Graft Construct
• A five-layer multi-laminate small diameter vascular graft containing multiple layers of tubular juvenile small intestinal submucosa was implanted into the left anterior descending coronary artery of a 70-pound dog. The graft was anastomosed in with a running 7-0 prolene suture, with each suture spaced about 1-2 mm apart. Upon removal of the clamps, normal blood flow was re-established, and the graft was patent with no apparent leaking from the anastomosis or dilatation.
• While the invention has been illustrated and described in detail in the drawings and foregoing description, the same is to be considered as illustrative and not restrictive in character, it being understood that only the preferred embodiment has been shown and described and that all changes and modifications that come within the spirit of the invention are desired to be protected. In addition, all publications cited herein are hereby incorporated by reference in their entirety as if each had been individually incorporated by reference and fully set forth.

Claims (40)

1. A tubular graft construct, comprising:

a tubular element having walls and a lumen; and

said walls including juvenile submucosa tissue from a warm-blooded vertebrate.

2. A tubular graft construct ofclaim 1, wherein said juvenile submucosa tissue retains a natural, intact tubular form.

3. A tubular graft construct ofclaim 2, wherein said juvenile submucosa tissue is juvenile small intestinal submucosa tissue, and wherein a surface of said lumen is defined by said juvenile small intestinal submucosa tissue.

4. A tubular graft construct ofclaim 1, wherein said walls include a layer provided by said juvenile submucosa tissue, and at least one additional layer.

5. A tubular graft construct ofclaim 4, wherein a surface of said lumen is defined by said submucosa tissue.

6. A tubular graft construct ofclaim 5, wherein said at least one additional layer includes a collagen layer.

7. A tubular graft construct ofclaim 6, wherein said collagen layer is a naturally derived collagen layer.

8. A tubular graft construct ofclaim 7, wherein said naturally derived collagen layer is an extracellular matrix layer.

9. A tubular graft construct ofclaim 5, wherein said at least one additional layer includes a synthetic layer.

10. A tubular graft construct ofclaim 9, wherein said synthetic layer is comprised of a synthetic polymer.

11. A tubular graft construct ofclaim 8, wherein said extracellular matrix layer comprises submucosa, dura mater, pericardium, serosa, peritoneum, or basement membrane.

12. A tubular graft construct ofclaim 11, wherein said extracellular matrix layer comprises submucosa.

13. A tubular graft construct ofclaim 12, wherein said submucosa is mammalian submucosa.

14. A tubular graft construct ofclaim 13, wherein said mammalian submucosa is porcine, bovine, or ovine submucosa.

15. A tubular graft construct ofclaim 14, wherein said submucosa is porcine submucosa.

16. A tubular graft construct ofclaim 15, wherein said porcine submucosa is adult porcine submucosa.

17. A tubular graft construct ofclaim 15, wherein said porcine submucosa is a second layer of juvenile porcine submucosa.

18. A tubular graft construct according toclaim 17, wherein said second layer of juvenile porcine submucosa constitutes an outermost layer of the construct.

19. A tubular graft construct ofclaim 18, comprising:

an innermost layer defining a surface of the lumen, the innermost layer provided by juvenile small intestinal submucosa tissue retaining a natural, intact tubular form;

at least one intermediate layer; and

an outermost layer provided by juvenile small intestinal submucosa tissue retaining a natural, intact tubular form.

20. A tubular graft construct ofclaim 19, wherein said at least one intermediate layer includes a collagenous layer.

21. A tubular graft construct ofclaim 20, wherein said collagenous layer is an extracellular matrix layer.

22. A tubular graft construct ofclaim 21, wherein said extracellular matrix layer is submucosa.

23. A tubular graft construct ofclaim 22, wherein said submucosa is small intestinal submucosa.

24. A tubular graft construct ofclaim 23, wherein said small intestinal submucosa is adult small intestinal submucosa.

25. A tubular graft construct ofclaim 24, wherein said adult small intestinal submucosa is porcine small intestinal submucosa.

26. A tubular graft construct ofclaim 4, wherein said juvenile submucosa layer and at least one additional layer are bonded to one another.

27. A tubular graft construct ofclaim 19, wherein said innermost layer, at least one intermediate layer, and outermost layer are bonded to one another.

28. A tubular graft construct ofclaim 1, wherein said lumen has an anti-thrombogenic coating.

29. A tissue graft composition, comprising juvenile submucosa tissue.

30. A tissue graft composition ofclaim 29, wherein said submucosa tissue is small intestinal submucosa tissue.

31. A tissue graft composition ofclaim 30, wherein said small intestinal submucosa tissue retains an intact, tubular form.

32. A tissue graft composition ofclaim 31, wherein said tubular form has a diameter not exceeding about 8 mm.

33. A tissue graft composition ofclaim 29, wherein said submucosa tissue is porcine, bovine, or ovine.

34. A method for tissue grafting in a mammal, comprising grafting said mammal with a tissue graft material comprising juvenile submucosa tissue.

35. A method ofclaim 34, wherein said juvenile submucosa tissue retains an intact, tubular form.

36. A method ofclaim 35, wherein said tubular form has a diameter not exceeding about 12 mm.

37. A method ofclaim 34, wherein said juvenile submucosa tissue is porcine, bovine, or ovine.

38. A tubular graft construct, comprising:

a tubular element having walls and a lumen;

said walls including at least a first layer formed with intact tubular submucosa having a native internal diameter no greater than about 12 mm.

39. A tubular graft construct ofclaim 38, wherein said walls include at least a second layer.

40. A tubular graft construct ofclaim 39, wherein said second layer includes submucosa tissue.

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