TWI696699B

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Info

Publication number: TWI696699B
Application number: TW107145994A
Authority: TW
Inventors: 高志明; 林韋翰; 葉雅文; 陳師慶; 簡志青; 許藝騰
Original assignee: 國立中山大學
Priority date: 2018-12-19
Filing date: 2018-12-19
Publication date: 2020-06-21
Also published as: TW202024318A

Abstract

A method of degrading a chlorine-containing organic substance includes steps of: providing a medium comprising a chlorine-containing organic substance, a sulfate, a dechlorination bacteria, and a sulfate-reducing bacteria bacteria, wherein the chlorine-containing organic substance comprises trichloroethylene, and the dechlorination bacteria comprisesDehalococcoides sp.; and a ferric ion salt is applied to the medium to inhibit an activity of the sulfate-reducing bacteria, thereby improving an efficiency of dechlorination of the chlorine-containing organic substance.

Description

Translated fromChinese

降解含氯有機物的方法Method for degrading chlorine-containing organic matter

本發明係關於一種生物復育方法，特別是關於一種降解含氯有機物的方法。The present invention relates to a method of biological restoration, in particular to a method of degrading chlorine-containing organic matter.

含氯化合物的生物復育法有兩種，一種是透過天然的機制，污染物以自然衰減的方式，降解為毒性較小或是無毒性的物質。另一種生物復育方式是透過添加有利的物質，活化生物系統中的微生物，以達到強化天然衰減的目的。There are two methods of biological reincubation of chlorinated compounds. One is through a natural mechanism. The pollutants are degraded into less toxic or non-toxic substances by natural attenuation. Another way of biological restoration is to activate the microorganisms in the biological system by adding beneficial substances to achieve the purpose of strengthening natural attenuation.

現有的降解含氯有機物的方法，主要是通過脫氯菌的作用，來使含氯有機物降解為其他對環境較無害的物質(例如二氧化碳、甲烷或乙烯等)。然而，由於這種方式往往會受到其他菌種的干擾，導致無法達到預期的效果，甚至反而產生對人體更為有害的物質(例如順式二氯乙烯或氯乙烯等物質)。Existing methods for degrading chlorine-containing organic substances mainly degrade chlorine-containing organic substances into other substances that are less harmful to the environment (such as carbon dioxide, methane, or ethylene) through the action of dechlorinating bacteria. However, because this method is often interfered by other bacteria, it can not achieve the desired effect, and even produces substances that are more harmful to the human body (such as cis dichloroethylene or vinyl chloride).

故，有必要提供一種降解含氯有機物的方法，以解決習用技術所存在的問題。Therefore, it is necessary to provide a method of degrading chlorine-containing organic substances to solve the problems in the conventional technology.

本發明之一目的在於提供一種降解含氯有機物的方法，其係利用特定的化合物抑制特定菌種，以改善脫氯菌的作用效果。An object of the present invention is to provide a method for degrading chlorine-containing organic substances, which utilizes specific compounds to inhibit specific bacterial species to improve the dechlorination bacteria's effect.

為達上述之目的，本發明提供一種降解含氯有機物的方法，其包含步驟：提供一介質，該介質包括一含氯有機物、硫酸鹽、一脫氯菌以及一硫酸還原菌，其中該含氯有機物包含三氯乙烯，以及該脫氯菌包含一Dehalococcoides屬菌；以及施加三價鐵離子鹽類至該介質中，以抑制該硫酸還原菌的活性，進而提高該脫氯菌降解該含氯有機物的效率。To achieve the above objective, the present invention provides a method for degrading chlorine-containing organic matter, which comprises the steps of: providing a medium including a chlorine-containing organic matter, sulfate, a dechlorinating bacterium, and a sulfate-reducing bacterium, wherein the chlorine-containing Organic matter contains trichloroethylene, and the dechlorination bacteria contains a genusDehalococcoides ; and ferric ion salts are applied to the medium to inhibit the activity of the sulfuric acid reducing bacteria, thereby improving the dechlorination bacteria to degrade the chlorine-containing organic matter s efficiency.

在本發明之一實施例中，該介質包含堆肥、蔗渣、牛糞、落葉及雜草中的至少一種。In one embodiment of the present invention, the medium includes at least one of compost, bagasse, cow dung, fallen leaves, and weeds.

在本發明之一實施例中，該介質係源自於受該含氯污染物污染的一場址。In one embodiment of the invention, the medium is derived from a site contaminated with the chlorine-containing pollutant.

在本發明之一實施例中，在施加三價鐵離子鹽類至該介質中的步驟中，更包含加入一碳源至該介質中。In one embodiment of the present invention, the step of applying ferric ion salts to the medium further includes adding a carbon source to the medium.

在本發明之一實施例中，該碳源包含γ-聚麩胺酸。In one embodiment of the present invention, the carbon source contains γ-polyglutamic acid.

在本發明之一實施例中，該三價鐵離子鹽類包含氧化鐵與檸檬酸鐵中的至少一種。In one embodiment of the present invention, the ferric ion salts include at least one of iron oxide and iron citrate.

在本發明之一實施例中，該三價鐵離子鹽類的一濃度係介於0.2mM至0.6mM之間。In one embodiment of the present invention, a concentration of the ferric ion salts is between 0.2 mM and 0.6 mM.

在本發明之一實施例中，該脫氯菌還包含Desulfitobacterium屬菌、Dehalobacter屬菌、Desulfuromonas屬菌及Geobacter屬菌中的至少一種。In one embodiment of the present invention, further comprising the dechlorinationDesulfitobacterium bacteria belonging to the genus, belonging to the genusDehalobacter,Desulfuromonas genus bacteria and at least one bacterium of the genusGeobacter.

在本發明之一實施例中，該Dehalococcoides屬菌包含195菌種、CBDB1菌種、BAV1菌種、FL-2菌種及GT菌種中的至少一種。In one embodiment of the present invention, theDehalococcoides genus includes at least one of 195 strains, CBDB1 strains, BAV1 strains, FL-2 strains, and GT strains.

在本發明之一實施例中，該含氯有機物還包含四氯乙烯。In one embodiment of the present invention, the chlorine-containing organic substance further includes tetrachloroethylene.

10‧‧‧方法10‧‧‧Method

11‧‧‧步驟11‧‧‧Step

12‧‧‧步驟12‧‧‧Step

第1圖：本發明一實施例之降解含氯有機物的方法之流程示意圖。Figure 1: Schematic diagram of a method for degrading chlorine-containing organic matter according to an embodiment of the present invention.

為了讓本發明之上述及其他目的、特徵、優點能更明顯易懂，下文將特舉本發明較佳實施例，並配合所附圖式，作詳細說明如下。再者，本發明所提到的方向用語，例如上、下、頂、底、前、後、左、右、內、外、側面、周圍、中央、水平、橫向、垂直、縱向、軸向、徑向、最上層或最下層等，僅是參考附加圖式的方向。因此，使用的方向用語是用以說明及理解本發明，而非用以限制本發明。In order to make the above and other objects, features, and advantages of the present invention more obvious and understandable, the preferred embodiments of the present invention will be specifically described below in conjunction with the accompanying drawings, which are described in detail below. Furthermore, the direction words mentioned in the present invention, such as up, down,Top, bottom, front, back, left, right, inner, outer, side, surrounding, center, horizontal, horizontal, vertical, vertical, axial, radial, uppermost or lowermost layer, etc., only for reference to additional drawings direction. Therefore, the directional terminology is used to illustrate and understand the present invention, not to limit the present invention.

請參照第1圖所示，本發明一實施例之降解含氯有機物的方法10主要包含下列步驟11及12：提供一介質，該介質包括一含氯有機物、硫酸鹽、一脫氯菌以及一硫酸還原菌，其中該含氯有機物包含三氯乙烯，以及該脫氯菌包含一Dehalococcoides屬菌(步驟11)；以及施加三價鐵離子鹽類至該介質中，以抑制該硫酸還原菌的活性，進而提高該脫氯菌降解該含氯有機物的效率(步驟12)。本發明將於下文詳細說明實施例之上述各步驟的實施細節及其原理。Referring to FIG. 1, amethod 10 for degrading chlorine-containing organic matter according to an embodiment of the present invention mainly includes the followingsteps 11 and 12: providing a medium including a chlorine-containing organic matter, sulfate, a dechlorinating bacterium, and a Sulfate-reducing bacteria, wherein the chlorine-containing organic matter contains trichloroethylene, and the dechlorination bacteria contains aDehalococcoides genus (step 11); and applying ferric ion salts to the medium to inhibit the activity of the sulfate-reducing bacteria To further increase the efficiency of the dechlorination bacteria to degrade the chlorine-containing organic matter (step 12). The present invention will describe in detail the implementation details and principles of the above steps in the embodiments below.

本發明一實施例之降解含氯有機物的方法10首先係步驟11：提供一介質，該介質包括一含氯有機物、硫酸鹽、一脫氯菌以及一硫酸還原菌，其中該含氯有機物包含三氯乙烯，以及該脫氯菌包含一Dehalococcoides屬菌。在本步驟11中，該介質例如包含堆肥、蔗渣、牛糞、落葉及雜草中的至少一種。在一實施例中，該介質係源自於受該含氯污染物污染的一場址。在另一實施例中，該含氯有機物還可包含四氯乙烯。Amethod 10 for degrading a chlorine-containing organic substance according to an embodiment of the present invention is first step 11: providing a medium including a chlorine-containing organic substance, a sulfate, a dechlorinated bacterium, and a sulfate-reducing bacterium, wherein the chlorine-containing organic substance includes three Vinyl chloride, and the dechlorination bacteria contain a genusDehalococcoides . In thisstep 11, the medium includes, for example, at least one of compost, bagasse, cow dung, fallen leaves, and weeds. In one embodiment, the medium is derived from a site contaminated with the chlorine-containing pollutant. In another embodiment, the chlorine-containing organic matter may further include tetrachloroethylene.

在一實施例中，該脫氯菌還包含Desulfitobacterium屬菌、Dehalobacter屬菌、Desulfuromonas屬菌及Geobacter屬菌中的至少一種。在又一實施例中，該Dehalococcoides屬菌包含195菌種、CBDB1菌種、BAV1菌種、FL-2菌種及GT菌種中的至少一種(上述Dehalococcoides屬菌中的各菌種的定序可見於下列文獻中：Lee,P.K.,Cheng,D.,West,K.A.,Alvarez-Cohen,L.,& He,J.(2013).Isolation of two new Dehalococcoides mccartyi strains with dissimilar dechlorination functions and their characterization by comparative genomics via microarray analysis.Environmental microbiology,15(8),2293-2305.)。In one embodiment, the dechlorination bacteria further include at least one of bacteria of the genusDesulfitobacterium ,bacteria of the genusDehalobacter , bacteria of the genusDesulfuromonas , and bacteria of the genusGeobacter . In yet another embodiment, theDehalococcoides genus includes at least one of 195 species, CBDB1, BAV1, FL-2, and GT species (sequencing of each species in theDehalococcoides genus described above) Can be found in the following documents: Lee, PK, Cheng, D., West, KA, Alvarez-Cohen, L., & He, J. (2013). Isolation of two new Dehalococcoides mccartyi strains with dissimilar dechlorination functions and their characterization by comparative genomics via microarray analysis. Environmental microbiology, 15(8), 2293-2305.).

本發明一實施例之降解含氯有機物的方法10最後係步驟12：施加三價鐵離子鹽類至該介質中，以抑制該硫酸還原菌的活性，進而提高該脫氯菌降解該含氯有機物的效率。在本步驟12中，還可加入一碳源(例如包含γ-聚麩胺酸)至該介質中。Amethod 10 for degrading chlorine-containing organic matter according to an embodiment of the present invention is finally step 12: applying ferric ion salts to the medium to inhibit the activity of the sulfuric acid-reducing bacteria, thereby improving the dechlorination bacteria to degrade the chlorine-containing organic matter s efficiency. In thisstep 12, a carbon source (for example, containing γ-polyglutamic acid) can also be added to the medium.

這邊要提到的是，硫酸鹽是地下水中主要陰離子之一，對於富含硫酸鹽的環境(例如250mg/L)，因為氯乙烯(vinyl chloride；VC)及順式二氯乙烯(cis-1,2-dichloroethene；cis-DCE)的氫氣閥值水平低於硫酸還原菌(sulfate reduction bacteria；SRB)的氫氣閥值水平，並且Dehalococcoides(Dhc)屬菌的氫氣閥值水平也低於硫酸還原菌的氫氣閥值水平，故硫酸還原菌可以穩定生長並導致Dhc屬菌無法完全還原脫氯，反而會造成氯乙烯(VC)及順式二氯乙烯(cis-DCE)的累積之問題。因此，本發明實施例之降解含氯有機物的方法主要是通過加入特定的化合物(三價鐵離子鹽類)抑制特定菌種(硫酸還原菌)的活性，進而提高該脫氯菌完全降解該含氯有機物的效率。It should be mentioned here that sulfate is one of the main anions in groundwater. For sulfate-rich environments (such as 250mg/L), vinyl chloride (VC) and cis-dichloroethylene (cis- The hydrogen threshold level of 1,2-dichloroethene (cis-DCE) is lower than that of sulfur reduction bacteria (SRB), and the hydrogen threshold level ofDehalococcoides (Dhc ) bacteria is also lower than that of sulfuric acid reduction The hydrogen threshold level of bacteria, so sulfate-reducing bacteria can grow steadily and causeDhc genus bacteria to not be fully reduced and dechlorinated. Instead, it will cause the accumulation of vinyl chloride (VC) and cis-dichloroethylene (cis-DCE). Therefore, the method for degrading chlorine-containing organic substances in the embodiments of the present invention is mainly to inhibit the activity of specific bacterial species (sulfate-reducing bacteria) by adding specific compounds (ferric ion salts), thereby improving the complete degradation of the dechlorinated bacteria. Chlorine efficiency.

另外要提到的是，由於該三價鐵離子鹽類中的三價鐵的氧化還原電位(Fe³⁺/Fe²⁺：+0.77V)比硫酸鹽(SO₄^2-/HS^-：-0.22V)和二氧化碳(CO₂/CH₄：-0.24V)高，因此電子接受能力也較強，所以可生成亞鐵離子以與硫化物結合成不溶性硫化鐵(FeS)，進而使硫酸還原菌受到抑制作用。It should also be mentioned that the oxidation-reduction potential (Fe³⁺ /Fe²⁺ : +0.77V) of ferric iron in the ferric ion salts is higher than that of sulfate (SO₄^2- /HS^- :- 0.22V) and carbon dioxide (CO₂ /CH₄ : -0.24V) are high, so the electron accepting ability is also strong, so it can generate ferrous ions to combine with the sulfide to form insoluble iron sulfide (FeS), and then make sulfuric acid reducing bacteria Being inhibited.

以下將提出數個實施例，以說明本發明實施例之降解含氯有機物的方法10確實具有去除含氯有機物(特別是三氯乙烯)並且減少有害物質生成(例如順式二氯乙烯或氯乙烯等物質)的效果。Several examples will be presented below to illustrate that themethod 10 for degrading chlorine-containing organic substances according to an embodiment of the present invention does have the effect of removing chlorine-containing organic substances (especially trichloroethylene) and reducing the generation of harmful substances (such as cis-vinyl chloride or vinyl chloride) Etc.).

實施例1Example 1

首先提供含有一含氯有機物、硫酸鹽、一脫氯菌以及一硫酸還原菌的介質，將11克的該介質注入90mL的地下水中，其中該地下水內含濃度為250mg/L的硫酸鐵以及濃度為0.2mM的檸檬酸鐵。另一方面，再加入2.5mL的γ-聚麩胺酸作為碳源。First, provide a medium containing a chlorine-containing organic matter, sulfate, dechlorination bacteria, and a sulfate-reducing bacteria. Inject 11 grams of the medium into 90 mL of groundwater, where the groundwater contains 250 mg/L of ferric sulfate and its concentration It is 0.2 mM ferric citrate. On the other hand, 2.5 mL of γ-polyglutamic acid was added as a carbon source.

實施例2與3Examples 2 and 3

實施例2與3大致相同於實施例1，惟其不同之處在於檸檬酸鐵的濃度分別為0.4mM與0.6mM。Examples 2 and 3 are substantially the same as Example 1, except that the concentration of iron citrate is 0.4 mM and 0.6 mM, respectively.

比較例1Comparative example 1

比較例1大致相同於實施例1，惟其不同之處在於不添加檸檬酸鐵。Comparative Example 1 is roughly the same as Example 1, except that it does not add ferric citrate.

接下來針對實施例1至3與比較例1進行分析比較。首先，分析在經過40天降解過成後，實施例1至3與比較例1的含氯有機物中的三氯乙烯的殘餘百分比。根據分析結果，實施例1的降解效率為70.44%、實施例2的降解效率為70.04%及實施例3的降解效率為85.36%。而比較例1的降解效率為97.07%。根據此分析結果可知，實施例1至3與比較例1的三氯乙烯的降解效率皆具明顯效果。Next, Examples 1 to 3 and Comparative Example 1 are analyzed and compared. First, the residual percentage of trichloroethylene in the chlorine-containing organics of Examples 1 to 3 and Comparative Example 1 after 40 days of degradation was analyzed. According to the analysis results, the degradation efficiency of Example 1 is 70.44%, the degradation efficiency of Example 2 is 70.04%, and the degradation efficiency of Example 3 is 85.36%. The degradation efficiency of Comparative Example 1 was 97.07%. According to the analysis results, it can be seen that the degradation efficiency of trichloroethylene in Examples 1 to 3 and Comparative Example 1 has obvious effects.

然而，根據進一步分析氯乙烯(VC)及順式二氯乙烯(cis-DCE)的濃度可知，比較例1的氯乙烯濃度約為1.2mg/L，而順式二氯乙烯約為0.5mg/L。實施例1的氯乙烯濃度約為0.3mg/L，而順式二氯乙烯接近為0；實施例2的氯乙烯濃度約為0.9mg/L，而順式二氯乙烯接近為0；及實施例3的氯乙烯濃度約為1mg/L，而順式二氯乙烯接近為0.3mg/L。由此可見，通過加入三價鐵離子鹽類的加入，確實具有去除含氯有機物(特別是三氯乙烯)並且減少有害物質生成(例如順式二氯乙烯或氯乙烯等物質)的效果，亦即實施例1至3完全降解含氯有機物的效率遠高於比較例1。However, according to further analysis of the concentrations of vinyl chloride (VC) and cis-dichloroethylene (cis-DCE), it can be seen that the vinyl chloride concentration of Comparative Example 1 is about 1.2 mg/L, while the cis-dichloroethylene is about 0.5 mg/L L. The vinyl chloride concentration of Example 1 is about 0.3 mg/L, and cis-vinyl chloride is close to 0; the vinyl chloride concentration of Example 2 is about 0.9 mg/L, and cis-vinyl chloride is close to 0; and implementation The concentration of vinyl chloride in Example 3 is about 1 mg/L, while cis-dichloroethylene is close to 0.3 mg/L. It can be seen that the addition of ferric ion salts does have the effect of removing chlorine-containing organic substances (especially trichloroethylene) and reducing the generation of harmful substances (such as cis dichloroethylene or vinyl chloride). That is, the efficiency of Examples 1 to 3 in completely degrading chlorine-containing organic substances is much higher than that in Comparative Example 1.

另一方面，針對實施例1與3分析經過10天與30天的氫氣數據，其中實施例1及3在10天的氫氣數據分別為3.029mg/L及3.690mg/L，而在30天的氫氣數據則兩者皆大約為0。由此可見，在經過30天後，氫氣產出馬上被硫酸還原菌及其他菌種所利用，因此氫氣幾乎完全消耗。On the other hand, the hydrogen data after 10 days and 30 days were analyzed for Examples 1 and 3, wherein the hydrogen data of Examples 1 and 3 at 10 days were 3.029 mg/L and 3.690 mg/L, respectively, while at 30 days The hydrogen data is approximately 0 for both. It can be seen that after 30 days, hydrogen production is utilized by sulfate-reducing bacteria and other bacterial species, so the hydrogen is almost completely consumed.

另外，針對實施例1與3分析一開始(0天)與經過30天的硫酸還原菌的數量，實施例1從5×10¹¹基因複製/升(gene copies/L)下降到10⁹gene copies/L，實施例3從5×10¹¹gene copies/L下降到3×10⁸gene copies/L。由此可知，實施例1與3的硫酸還原菌皆有明顯下降。In addition, for the analysis of the number of sulfuric acid-reducing bacteria at the beginning (day 0) and after 30 days for Examples 1 and 3, Example 1 decreased from 5×10¹¹ gene copies/liter (gene copies/L) to 10⁹ gene copies /L, Example 3 decreased from 5×10¹¹ gene copies/L to 3×10⁸ gene copies/L. From this, it can be seen that the sulfate-reducing bacteria of Examples 1 and 3 have significantly decreased.

綜上所述，本發明實施例的降解含氯有機物的方法，是通過利用特定的化合物(三價鐵離子鹽類)抑制特定菌種(硫酸還原菌)，以提高該脫氯菌完全降解該含氯有機物的效率。In summary, the method for degrading chlorine-containing organic substances in the embodiments of the present invention is to inhibit specific bacteria (sulfuric acid reducing bacteria) by using specific compounds (ferric ion salts) to improve the complete degradation of the dechlorinated bacteria. The efficiency of chlorine-containing organics.

雖然本發明已以較佳實施例揭露，然其並非用以限制本發明，任何熟習此項技藝之人士，在不脫離本發明之精神和範圍內，當可作各種更動與修飾，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。Although the present invention has been disclosed in preferred embodiments, it is not intended to limit the present invention. Anyone who is familiar with this skill can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the present invention The scope of protection shall be deemed as defined by the scope of the attached patent application.

10‧‧‧方法10‧‧‧Method

11‧‧‧步驟11‧‧‧Step

12‧‧‧步驟12‧‧‧Step

Claims

Translated fromChinese

一種降解含氯有機物的方法，其包含步驟：提供一介質，該介質包括一含氯有機物、硫酸鹽、一脫氯菌以及一硫酸還原菌，其中該含氯有機物包含三氯乙烯，以及該脫氯菌包含一Dehalococcoides屬菌；以及施加三價鐵離子鹽類至該介質中，以抑制該硫酸還原菌的活性，進而提高該脫氯菌降解該含氯有機物的效率。A method for degrading chlorine-containing organic matter, comprising the steps of: providing a medium comprising a chlorine-containing organic matter, a sulfate, a dechlorinating bacterium and a sulfate-reducing bacterium, wherein the chlorine-containing organic matter comprises trichloroethylene, and the dechlorination The chlorine bacteria include aDehalococcoides genus; and ferric ion salts are applied to the medium to inhibit the activity of the sulfuric acid-reducing bacteria, thereby improving the efficiency of the dechlorination bacteria to degrade the chlorine-containing organic matter.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該介質包含堆肥、蔗渣、牛糞、落葉及雜草中的至少一種。The method for degrading chlorine-containing organic matter as described in item 1 of the patent application scope, wherein the medium contains at least one of compost, bagasse, cow dung, fallen leaves and weeds.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該介質係源自於受該含氯污染物污染的一場址。The method for degrading chlorine-containing organic matter as described in item 1 of the patent application scope, wherein the medium is derived from a site polluted by the chlorine-containing pollutant.

如申請專利範圍第1項所述之降解含氯有機物的方法，在施加三價鐵離子鹽類至該介質中的步驟中，更包含加入一碳源至該介質中。The method for degrading chlorine-containing organic substances as described in item 1 of the patent application scope, in the step of applying ferric ion salts to the medium, further includes adding a carbon source to the medium.

如申請專利範圍第4項所述之降解含氯有機物的方法，其中該碳源包含γ-聚麩胺酸。The method for degrading chlorine-containing organic matter as described in item 4 of the patent application scope, wherein the carbon source includes γ-polyglutamic acid.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該三價鐵離子鹽類包含氧化鐵與檸檬酸鐵中的至少一種。The method for degrading a chlorine-containing organic substance as described in item 1 of the patent application scope, wherein the ferric ion salt contains at least one of iron oxide and iron citrate.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該三價鐵離子鹽類的一濃度係介於0.2mM至0.6mM之間。The method for degrading chlorine-containing organic substances as described in item 1 of the patent application scope, wherein a concentration of the ferric ion salts is between 0.2 mM and 0.6 mM.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該脫氯菌還包含Desulfitobacterium屬菌、Dehalobacter屬菌、Desulfuromonas屬菌及Geobacter屬菌中的至少一種。The application methods for degrading chlorinated organic compounds in item 1 of the scope of the patent, wherein the dechlorination bacteria belonging to the genus further comprisingDesulfitobacterium,Dehalobacter belonging to the genus, the genusDesulfuromonas bacteria and at least one bacterium of the genusGeobacter.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該Dehalococcoides屬菌包含195菌種、CBDB1菌種、BAV1菌種、FL-2菌種及GT菌種中的至少一種。The method for degrading a chlorine-containing organic substance as described in item 1 of the patent application scope, wherein theDehalococcoides genus includes at least one of 195 species, CBDB1 strain, BAV1 strain, FL-2 strain and GT strain.

如申請專利範圍第1項所述之降解含氯有機物的方法，其中該含氯有機物還包含四氯乙烯。The method for degrading chlorine-containing organic matter as described in item 1 of the patent application scope, wherein the chlorine-containing organic matter further comprises tetrachloroethylene.