本發明有關一種殺蟲劑的藥效測試方法，特別是指一種針對具有固型物滅菌藥劑的水蒸式煙燻殺蟲劑測試方法。The invention relates to a method for testing the efficacy of an insecticide, in particular to a method for testing a water-steamed smoked insecticide having a solid sterilization agent.
歐美國家至今只生產傳統「點火引燃式的煙霧劑」(Smoke Generator)或研發「全釋型高壓噴霧煙霧劑」(Aerosol Total release Smoke Generator)。此種煙物劑或噴霧煙物劑的滅菌劑一般皆以液劑為主。In Europe and the United States, only the traditional "Smoke Generator" or the "Aerosol Total release Smoke Generator" has been produced. The sterilizing agent of such a tobacco agent or a sprayed tobacco agent is generally based on a liquid agent.
傳統滅菌液劑的殺菌測試方法是依據處方標示配製的Tryptone Soy Agar和Potato Dextrose Agar，於121℃，15磅高壓下滅菌15分鐘，冷卻至約50℃，倒入無菌培養皿中，每一皿倒入約15mL的培養基，待其冷卻凝固，在30℃下放置24小時，檢查有無雜菌生長。再配製無菌稀釋液：1.磷酸二氫鉀溶液：取3.4g磷酸二氫鉀(KH2PO4)溶於50mL之蒸餾水中，待完全溶解後，以1.0N氫氧化鈉溶液調整其PH值為7.2±0.5。然後加蒸餾水至全量為100mL，儲存於冰箱中做為母液備用；2.氯化鎂溶液：取8.1g氯化鎂(MgCl2‧6H2O)，先溶於少量蒸餾水中，待完全溶解後，再加蒸餾水至全量為100ml，儲存於冰箱中做為母液備用；分別取10ml氯化鎂溶液和2.5ml磷酸二氫鉀溶液，再加入蒸餾水至全量為2000ml，混合均勻後分散於稀釋瓶中，經121℃滅菌15分鐘，做為無菌稀釋液備用。另取隔夜平板培養的測試菌，以無菌稀釋液做成懸浮菌液備用，取0.1ml懸浮菌液加到10ml的藥液中混合均勻，靜置作用10分鐘，取0.2ml塗抹於Tryptone soy agar或是Potato Detrose Agar於35±2℃下培養48±1小時後計算菌數，真菌則置於30±2℃培養箱中，經5天後計算菌落數；最後做測試菌株之總活菌數。The sterilization test method of the traditional sterilizing agent is based on the prescription labeling Tryptone Soy Agar and Potato Dextrose Agar, sterilized at 121 ° C, 15 psi for 15 minutes, cooled to about 50 ° C, and poured into a sterile Petri dish. Pour about 15 mL of the medium, wait for it to cool and solidify, and let it stand at 30 ° C for 24 hours to check for the growth of bacteria. Reconstitute the sterile diluent: 1. Potassium dihydrogen phosphate solution: Take 3.4g potassium dihydrogen phosphate (KH2 PO4 ) dissolved in 50mL of distilled water, after completely dissolved, adjust the PH value with 1.0N sodium hydroxide solution. It is 7.2 ± 0.5. Then add distilled water to the whole amount of 100mL, stored in the refrigerator as a mother liquor reserve; 2. Magnesium chloride solution: take 8.1g magnesium chloride (MgCl2 ‧6H2 O), first dissolved in a small amount of distilled water, after completely dissolved, add distilled water The total amount is 100ml, stored in the refrigerator as a mother liquor; take 10ml magnesium chloride solution and 2.5ml potassium dihydrogen phosphate solution, then add distilled water to the whole amount of 2000ml, mix well, disperse in the dilution bottle, and sterilize at 121 °C. Minutes, as a sterile diluent for use. In addition, the test bacteria in overnight plate culture were prepared, and the suspension liquid was prepared as a sterile diluent. The 0.1 ml suspension solution was added to 10 ml of the drug solution and mixed uniformly. The solution was allowed to stand for 10 minutes, and 0.2 ml was applied to Tryptone soy agar. Or the population of Potato Detrose Agar was cultured at 35±2°C for 48±1 hours, and the fungus was placed in a 30±2°C incubator. After 5 days, the number of colonies was counted. Finally, the total viable count of the test strain was calculated. .
傳統噴霧殺蟲劑之殺菌試驗方法是處方標示配製Potato Dextrose Agar及三分之一強度的Tryptone Soy Agar，於121℃，15磅高壓下滅菌15分鐘，冷卻至約50℃，倒入無菌培養皿中，每一皿倒入約15mL的培養基，待其冷卻凝固，在30℃下放置24小時，檢查有無雜菌生長；再取0.1ml菌液加到10ml的藥液中混合均勻，靜置作用10分鐘，取0.2ml塗抹於Potato Dextrose Agar或三分之一強度的Tryptone Soy Agar，每處理各做5次重覆，細菌置於35±2℃培養箱中，48±1小時計算菌數，真菌則置於30±2℃培養箱中，經5天後計算菌數；最後做測試菌株之總活菌數。The traditional bactericidal test method for spray insecticides is to formulate Potato Dextrose Agar and one-third strength Tryptone Soy Agar, sterilize at 121 ° C, 15 psi for 15 minutes, cool to about 50 ° C, and pour into a sterile Petri dish. Into each dish, pour about 15mL of medium, wait for it to cool and solidify, and let it stand at 30 ° C for 24 hours to check for the growth of bacteria; then add 0.1ml of bacteria solution to 10ml of liquid medicine to mix evenly, let stand 10 minutes, 0.2 ml applied to Potato Dextrose Agar or one-third strength Tryptone Soy Agar, each treatment was repeated 5 times, the bacteria were placed in a 35 ± 2 ° C incubator, 48 ± 1 hour to calculate the number of bacteria, The fungus was placed in a 30 ± 2 ° C incubator, and the number of bacteria was counted after 5 days; finally, the total viable count of the test strain was made.
然而，一般亞洲國家常使用的水蒸式煙燻劑主要是以固型物進行氣化揮發，其殺蟲配方中大多以擊倒劑配合殺死劑使用，讓整體殺蟲功效提升，直到如台灣發明專利公開200913884號所揭示，水蒸式殺蟲煙燻劑已有達到擊倒、殺死及滅菌三效合一的複方製劑之前，先前尚無任何水蒸式煙燻劑有添加滅菌劑。However, water-steamed fumigants commonly used in Asian countries are mainly vaporized and volatilized by solids. Most of their insecticidal formulas are combined with a killer and a killer to enhance the overall insecticidal efficacy until Taiwan Invention Patent Publication No. 200913884 discloses that before the water-steaming insecticidal fuming agent has reached the compounding effect of knocking, killing and sterilizing three effects, there is no prior steaming fuming agent with added sterilizing agent. .
也由於三效合一煙燻劑需要採用固型化的藥粒，因此，煙燻劑本身的滅菌劑需要經由蒸發後形成微小粒子漂浮在空中後，降到表面進行滅菌，故無法採用傳統滅菌劑液劑或滅菌劑噴霧劑之接觸法去測試三合一水蒸式煙燻劑。Because the three-in-one smoker needs to use solidified granules, the sterilizing agent of the smoky agent itself needs to form tiny particles floating in the air after evaporation, and then descend to the surface for sterilization, so conventional sterilization cannot be used. The contact method of the liquid agent or the sterilizing agent spray is used to test the three-in-one water-steaming fuming agent.
有鑒於目前測試滅菌劑的方法大多都以液劑方式量測，無法適用於三合一水蒸式殺蟲煙燻劑，且目前尚無任何分析滅菌劑為固型物的方法，更無藥效試驗的方法與標準可遵循，因此，為能夠符合滅菌劑為固型物的實際使用狀態，去設計一種藥效測試方法實乃創新開發的重點。In view of the fact that most of the methods for testing sterilizing agents are measured by liquid dosage, they cannot be applied to the three-in-one steam-steaming insecticidal fuming agent, and there is currently no method for analyzing the sterilizing agent as a solid substance, and there is no medicine. The method and standard of the efficacy test can be followed. Therefore, in order to be able to conform to the actual use state of the sterilizing agent as a solid, it is the focus of innovative development to design a drug efficacy test method.
爰是，本發明之主要目的，旨在提供一種水蒸式煙燻劑的藥效測試方法，用以測試滅菌劑為固型物之水蒸式煙燻殺蟲劑，解決傳統測試方式僅能應用於液劑之困擾。Therefore, the main object of the present invention is to provide a method for testing the efficacy of a water-steaming fuming agent for testing a water-steaming smoked insecticide having a sterilizing agent as a solid, and solving the traditional test method can only Used in the trouble of liquid agents.
為達上揭目的，本發明藥效測試方法，主要是針對具有固型物滅菌劑的水蒸式煙燻劑開發創新，其包含以下步驟：In order to achieve the above, the efficacy test method of the present invention mainly aims at the development and innovation of a water-steaming fuming agent having a solid sterilizing agent, which comprises the following steps:
(A) 依處方標示配製胰蛋白大豆瓊脂培養基(Tryptone Soy Agar，TSA)及水洋菜培養基(Water Agar)，於高溫高壓下滅菌後冷卻凝固，於室溫下放置一段培養時間後檢視有無雜菌生長；其中，上述胰蛋白大豆瓊脂(TSA)培養基採用原始材料的三分之一強度，且高溫高壓下滅菌冷卻凝固狀態為121℃及15磅高壓下滅菌15分鐘並冷卻至約50℃凝固，而上述培養時間為24小時。(A) Prepare Tryptone Soy Agar (TSA) and Water Agar medium according to the prescription, sterilize under high temperature and high pressure, cool and solidify, and store at room temperature for a period of culture and then check for impurities. Bacterial growth; wherein the above-mentioned tryptic soy agar (TSA) medium adopts one-third strength of the original material, and is sterilized by high temperature and high pressure at 121 ° C and sterilized at 15 psi for 15 minutes and cooled to about 50 ° C for solidification. And the above culture time is 24 hours.
(B) 取磷酸二氫鉀溶完全溶解於蒸餾水中形成磷酸二氫鉀溶液，另取氯化鎂完全溶解於蒸餾水中形成氯化鎂溶液，將氯化鎂溶液和磷酸二氫鉀溶液配合蒸餾水均勻混合製成無菌稀釋液。(B) Dissolve potassium dihydrogen phosphate completely dissolved in distilled water to form potassium dihydrogen phosphate solution, and take magnesium chloride completely dissolved in distilled water to form magnesium chloride solution, and mix magnesium chloride solution and potassium dihydrogen phosphate solution with distilled water to make sterile dilution. liquid.
(C)將培養的測試菌以無菌稀釋液做連續稀釋，使菌數分別形成三種不同稀釋度菌液，取兩種稀釋度的菌液對無煙燻劑對照組進行抽氣過濾，另取兩種相同於無煙燻劑對照組稀釋度的菌液對煙燻劑試驗組進行抽氣過濾步驟；於一較佳實施利中，上述三種不同稀釋度菌液分別可為101、102及103CFU/mL。(C) The cultured test bacteria are serially diluted with sterile dilution solution, and the bacterial counts are respectively formed into three different dilutions of the bacterial liquid, and the two dilutions of the bacterial liquid are subjected to suction filtration of the smokeless fumigant control group, and two other two are taken. The bacterial liquid which is the same as the dilution of the smokeless fumigant control group is subjected to a suction filtration step of the smoked agent test group; in a preferred embodiment, the above three different dilutions of the bacterial liquid may be 101 , 102 and 10 respectively3 CFU/mL.
(D)吸取適當稀釋度的菌液以抽氣過濾方式，使菌均勻散布於濾膜上，將濾膜平貼於水洋菜培養基上，保持濾膜濕潤並維持測試菌株之活性；(D) sucking the appropriate dilution of the bacterial liquid by means of suction filtration, so that the bacteria are evenly spread on the filter membrane, and the filter membrane is flatly attached to the watering vegetable medium, keeping the filter membrane moist and maintaining the activity of the test strain;
(E)將煙燻室先以酒精表面消毒，把燻蒸試驗組貼有濾膜的水洋菜培養基打開且濾膜朝上，放入煙燻劑後將門關閉，當燻蒸劑的煙不再散出時，開始計時燻蒸時間，另把無燻蒸劑對照組貼有濾膜的水洋菜培養基打開且濾膜朝上，直接將門關閉，與燻蒸試驗組同步計時；其中，上述燻蒸時間為24小時。(E) Disinfect the smoking room with alcohol on the surface first, and open the watering vegetable medium with the filter membrane in the fumigation test group and the filter membrane is facing up. After the fuming agent is put in, the door is closed, when the fumigant smoke is no longer scattered. When the time is out, the time of fumigation is started, and the water-free vegetable medium with the filter is not opened in the non-fumigant control group, and the filter membrane is turned upward, the door is closed directly, and the time is synchronized with the fumigation test group; wherein the fumigation time is 24 hours. .
(F)燻蒸時間一到就開動抽氣馬達，將煙燻室內的煙抽出後開門取出無燻蒸劑對照組與燻蒸試驗組的水洋菜培養基，並把濾膜轉換至胰蛋白大豆瓊脂(TSA)培養基上，經由培育程序後，計算無燻蒸劑對照組的菌數，並計算燻蒸試驗組的菌數相互比較；於一較佳實施利中，上述培育程序是採用35±2℃的狀態下持續放置48小時。(F) When the fumigation time is up, the pumping motor is started, the smoke in the smoked room is taken out, the door is opened, the waterless vegetable medium of the fumigant control group and the fumigation test group is taken out, and the filter membrane is switched to the tryptic soy agar (TSA). On the medium, after the incubation procedure, the number of bacteria in the no fumigant control group is calculated, and the number of bacteria in the fumigating test group is calculated to be compared with each other; in a preferred embodiment, the above incubation procedure is in a state of 35±2 ° C.Keep on for 48 hours.
茲為便於更進一步對本發明之構造、使用及其特徵有更深一層明確、詳實的認識與瞭解，爰舉出較佳實施例，配合圖式詳細說明如下：本發明水蒸式煙燻劑的藥效測試方法如下：In order to facilitate further understanding and understanding of the structure, the use and the features of the present invention, the preferred embodiments are described in detail with reference to the drawings: the water-steaming fumigant of the present invention. The effectiveness test method is as follows:
1.藥劑成份及含量：1. Ingredients and content of the drug:
賽滅寧(Cypermethrin) 8.0%w/wCypermethrin 8.0% w/w
鄰-苯甲基對氯酚(O-Benzyl-p-chlorophenol) 5.0%w/wO-Benzyl-p-chlorophenol 5.0% w/w
2.包裝規格：20公克/罐2. Packing specification: 20 grams / can
3.測試濃度：依不同內容量使用於不同之空間(規格3、10、15、20公克/罐、使用空間分別為1~2坪、2~3坪、3~5坪、5~6坪)，此次實驗之燻蒸式空間為1.8×1.8×1.8公尺，故取3公克藥劑進行測試。3. Test concentration: used in different spaces according to different content (specification 3, 10, 15, 20 g / can, use space is 1~2 ping, 2~3 ping, 3~5 ping, 5~6 ping ), the fumigation space of this experiment was 1.8 × 1.8 × 1.8 meters, so take 3 grams of the drug for testing.
4.作用時間：緊閉門窗4小時。4. Action time: Close the doors and windows for 4 hours.
5.測試微生物：5. Testing microorganisms:
a. bacillus cereus BCRC 10603(仙人掌桿菌)B. bacillus cereus BCRC 10603 (Cactus bacillus)
b. Escherichia coli BCRC 10675(大腸桿菌)b. Escherichia coli BCRC 10675 (E. coli)
c. Pseudomonas aeruginosa BCRC 10994(綠膿桿菌)c. Pseudomonas aeruginosa BCRC 10994 (Pseudomonas aeruginosa)
d. Salmonella choleraesuis subsp.Choleraesuis BCEC 10744(沙門桿菌)d. Salmonella choleraesuis subsp.Choleraesuis BCEC 10744 (Salmonella)
e. Staphylococcus aereus subsp.Aureus BCRC 12657(金黃葡萄球菌)e. Staphylococcus aereus subsp. Aureus BCRC 12657 (Staphylococcus aureus)
6. 材料：6. Material:
Tryptone Soy Agar(Difco)Tryptone Soy Agar(Difco)
Water AgarWater Agar
濾膜0.45um cellulose ester membranes(Adventec MFC,Inc. USA)Membrane 0.45um cellulose ester membranes (Adventec MFC, Inc. USA)
抽氣過濾設備Pumping equipment
無菌稀釋液(依環保署公告之配方配製)Sterile dilution (formulated in accordance with the regulations of the Environmental Protection Agency)
天秤、秤量紙、藥匙、試管、量筒、微量吸管Libra, weighing paper, medicine spoon, test tube, measuring cylinder, micro straw
7. 方法：7. Method:
(1) 依處方標示配製三分之一強度的胰蛋白大豆瓊脂培養基(Tryptone Soy Agar，TSA)和水洋菜培養基(Water Agar)，於121℃，15磅高壓下滅菌15分鐘，冷卻至約50℃，倒入無菌培養皿中，每一皿倒入約15mL的培養基，待其冷卻凝固，在30℃下放置24小時，檢查有無雜菌生長。(1) Prepare one-third of the strength of Tryptone Soy Agar (TSA) and Water Agar (Water Agar) according to the prescription, sterilize at 121 ° C, 15 psi for 15 minutes, and cool to about Pour into a sterile Petri dish at 50 ° C, pour about 15 mL of the medium into each dish, wait for it to cool and solidify, and let it stand at 30 ° C for 24 hours to check for the growth of bacteria.
(2) 無菌稀釋液之配製：(2) Preparation of sterile diluent:
1. 磷酸二氫鉀溶液：取3.4g磷酸二氫鉀(KH2PO4)溶於50mL之蒸餾水中，待完全溶解後，以1.0N氫氧化鈉溶液調整其PH值為7.2±0.5；然後加蒸餾水至全量為100mL，儲存於冰箱中做為原液備用。1. Potassium dihydrogen phosphate solution: 3.4 g of potassium dihydrogen phosphate (KH2 PO4 ) was dissolved in 50 mL of distilled water. After completely dissolved, the pH was adjusted to 7.2 ± 0.5 with 1.0 N sodium hydroxide solution; Add distilled water to a total amount of 100 mL, and store it in the refrigerator as a stock solution.
2. 氯化鎂溶液：取8.1g氯化鎂(MgCl2‧6H2O)，先溶於少量蒸餾水中，待完全溶解後，再加蒸餾水至全量為100mL，儲存於冰箱中做為原液備用。2. Magnesium chloride solution: Take 8.1g of magnesium chloride (MgCl2 ‧6H2 O), first dissolve in a small amount of distilled water, and after completely dissolving, add distilled water to the whole amount of 100mL, and store it in the refrigerator as a stock solution.
分別取10mL氯化鎂溶液和2.5mL磷酸二氫鉀溶液，再加入蒸餾水至全量為2000mL，混合均勻後分裝於稀釋瓶中，經121℃滅菌15分鐘，做為無菌稀釋液備用。Take 10mL of magnesium chloride solution and 2.5mL of potassium dihydrogen phosphate solution respectively, then add distilled water to the whole amount of 2000mL, mix well and then pack in the dilution bottle, sterilize at 121 °C for 15 minutes, use as sterile diluent.
(3) 將隔夜平板培養的測試菌，以無菌稀釋液做連續稀釋，使菌數約分別為101、102及103CFU/mL不同稀釋度菌液。吸取1毫升適當稀釋度的菌液以抽氣過濾方式，使菌均勻散布於濾膜上，將濾膜平貼於水洋菜培養基上，水洋菜培養基在長時間測試實驗的過程中，可保持濾膜濕潤以維持測試菌株之活性，預備進行燻蒸試驗以及無燻蒸劑之對照組實驗。(3) The test bacteria cultured overnight were serially diluted with a sterile diluent, so that the number of bacteria was about 101 , 102 and 103 CFU/mL of different dilutions. Pipette 1 ml of the appropriate dilution of the bacterial solution by means of suction filtration, so that the bacteria are evenly spread on the filter membrane, and the filter membrane is flatly attached to the water agar culture medium, and the water agar culture medium can be in the process of long-term test experiment. The filter was moistened to maintain the activity of the test strain, and a fumigation test and a control experiment without a fumigant were prepared.
(4) 燻蒸試驗組：煙燻室先以75%酒精表面消毒，將貼有濾膜的水洋菜培養基打開，濾膜朝上，在煙燻室內排開，放入煙燻劑後將門關閉。當燻蒸劑的煙不再散出時(約需6分鐘)，開始計時4小時。燻蒸時間一到就開動抽氣馬達，將室內的煙抽出，約經15分鐘後開門取出水洋菜培養基，將濾膜轉換至三分之一強度的胰蛋白大豆瓊脂(TSA)培養基上，於35±2℃下經2天培養後，計算菌數。每測試菌為五重複。(4) Fumigation test group: the smoking room is first disinfected with 75% alcohol surface, the watery vegetable medium with the filter membrane is opened, the filter membrane is facing up, is arranged in the smoke room, and the door is closed after the fuming agent is placed. . When the fumigant smoke no longer scatters (about 6 minutes), start timing for 4 hours. When the fumigation time is up, the pumping motor is started, and the indoor smoke is taken out. After about 15 minutes, the water is removed from the water and the filter is transferred to one-third of the strength of tryptic soy agar (TSA) medium. After 2 days of culture at 35 ± 2 ° C, the number of bacteria was counted. Five replicates per test bacterium.
(5) 無燻蒸劑對照組；煙燻室先以75%酒精表面消毒，將貼有濾膜的水洋菜培養基打開，濾膜朝上，在煙燻室內排開，直接將門關閉。與燻蒸試驗組同步計時。燻蒸時間一到就開動抽氣馬達，將室內的煙抽出，約經15分鐘後開門取出水洋菜培養基，將濾膜轉換至三分之一強度的胰蛋白大豆瓊脂(TSA)培養基上，於35±2℃下經2天培養後，計算無燻蒸劑對照組的菌數，並比較煙燻試驗組的菌數。每測試菌為五重複。(5) No fumigant control group; the smoking room is first disinfected with 75% alcohol surface, and the water-repellent vegetable medium with the filter membrane is opened, the filter membrane is facing upwards, and is arranged in the smoke chamber, and the door is closed directly. Synchronized with the fumigation test group. When the fumigation time is up, the pumping motor is started, and the indoor smoke is taken out. After about 15 minutes, the water is removed from the water and the filter is transferred to one-third of the strength of tryptic soy agar (TSA) medium. After 2 days of culture at 35±2°C, the number of bacteria in the no fumigant control group was calculated, and the number of bacteria in the smoked test group was compared. Five replicates per test bacterium.
前述方法僅為方便舉例說明之用，並非加以限制，亦即，上述試驗方法亦依據實際狀況對培養時間、等待時間、燻蒸時間等各種不同的試驗條件進行微幅調整。The foregoing method is merely for convenience of illustration and is not limited, that is, the above test method also slightly adjusts various test conditions such as culture time, waiting time, and fumigation time according to actual conditions.
以下為單獨滅菌劑原體與各種不同三合一複合配方藥劑，應用前述方法進行之藥效試驗結果：
由前述列表可知，單獨滅菌劑原體沒有發煙劑應用本發明測試方法的滅菌效果為很差；另滅菌劑母粉與擊倒劑母粉、殺死劑母粉，先用粉對粉稀釋，再加入發煙劑，稀釋後之黏劑、溶劑及少量水，再一起攪拌混合並以冷風乾燥造粒成型再次乾燥之滅菌效果為最佳；而將滅菌劑、擊倒劑與殺死劑三劑原體一起混合攪拌，再次烘乾造粒再烘乾之滅菌效果為不穩定，時好時壞。It can be seen from the foregoing list that the sterilizing effect of the sterilizing agent precursor without the smog agent is very poor. The sterilizing agent mother powder and the knockdown agent mother powder and the killer mother powder are first diluted with powder. Adding a fuming agent, a diluted adhesive, a solvent and a small amount of water, stirring and mixing together, and drying in a cold air granulation to re-dry the sterilization effect is best; and the sterilizing agent, the knockdown agent and the killing agent The three doses of the original body are mixed and stirred together, and the sterilization effect of drying and granulating and drying again is unstable, and the time is good or bad.
綜上所述，本發明水蒸式煙燻劑的藥效測試方法以無菌稀釋液做連續稀釋，並以抽氣過濾步驟使菌均勻散布於濾膜上，進行燻蒸試驗以及無燻蒸劑之對照組實驗，可測得氣化揮發後滅菌劑固態粒子的滅菌效果，如此即可測試像是水蒸式煙燻殺蟲劑的滅菌劑固型物，解決傳統測試方式僅能應用於液劑之困擾In summary, the method for testing the efficacy of the water-steaming fumigant of the present invention is serially diluted with a sterile diluent, and the bacteria are uniformly dispersed on the filter membrane by a suction filtration step, and the fumigation test and the comparison of the fumigant-free agent are carried out. In the group experiment, the sterilization effect of the solid particles of the sterilizing agent after gasification and volatilization can be measured, so that the sterilizing agent solid like the steam-steaming insecticide can be tested, and the traditional testing method can only be applied to the liquid agent. Troubled
以上所舉實施例，僅用為方便說明本發明並非加以限制，在不離本發明精神範疇，熟悉此一行業技藝人士依本發明申請專利範圍及發明說明所作之各種簡易變形與修飾，均仍應含括於以下申請專利範圍中。The above embodiments are intended to be illustrative only, and are not intended to limit the scope of the present invention. It is included in the scope of the following patent application.
第1圖係本發明水蒸式煙燻劑的藥效測試方法之流程圖。BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart showing the method for testing the efficacy of the water-steamable fumigant of the present invention.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW100127870ATWI417045B (en) | 2011-08-05 | 2011-08-05 | Pharmacodynamic Test Method for Water - borne Smoke |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW100127870ATWI417045B (en) | 2011-08-05 | 2011-08-05 | Pharmacodynamic Test Method for Water - borne Smoke |
| Publication Number | Publication Date |
|---|---|
| TW201306738A TW201306738A (en) | 2013-02-16 |
| TWI417045Btrue TWI417045B (en) | 2013-12-01 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW100127870ATWI417045B (en) | 2011-08-05 | 2011-08-05 | Pharmacodynamic Test Method for Water - borne Smoke |
| Country | Link |
|---|---|
| TW (1) | TWI417045B (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10967010B2 (en) | 2016-07-13 | 2021-04-06 | 4D Pharma Plc | Compositions comprising bacterial strains |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4163038A (en)* | 1977-03-03 | 1979-07-31 | Earth Chemical Company, Limited | Fumigating method and apparatus |
| US4171340A (en)* | 1977-03-03 | 1979-10-16 | Earth Chemical Company, Ltd. | Fumigating apparatus and method |
| TW200913884A (en)* | 2007-09-21 | 2009-04-01 | Aerolead Internat Ltd | Disposable multi-function fumigant |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4163038A (en)* | 1977-03-03 | 1979-07-31 | Earth Chemical Company, Limited | Fumigating method and apparatus |
| US4171340A (en)* | 1977-03-03 | 1979-10-16 | Earth Chemical Company, Ltd. | Fumigating apparatus and method |
| US4171340B1 (en)* | 1977-03-03 | 1986-04-08 | ||
| TW200913884A (en)* | 2007-09-21 | 2009-04-01 | Aerolead Internat Ltd | Disposable multi-function fumigant |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10967010B2 (en) | 2016-07-13 | 2021-04-06 | 4D Pharma Plc | Compositions comprising bacterial strains |
| Publication number | Publication date |
|---|---|
| TW201306738A (en) | 2013-02-16 |
| Publication | Publication Date | Title |
|---|---|---|
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