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TWI253957B - Apparatus for processing biochemical sample - Google Patents

Apparatus for processing biochemical sample
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Publication number
TWI253957B
TWI253957BTW094103773ATW94103773ATWI253957BTW I253957 BTWI253957 BTW I253957BTW 094103773 ATW094103773 ATW 094103773ATW 94103773 ATW94103773 ATW 94103773ATW I253957 BTWI253957 BTW I253957B
Authority
TW
Taiwan
Prior art keywords
hole
flange
tube
receiving groove
box
Prior art date
Application number
TW094103773A
Other languages
Chinese (zh)
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TW200628228A (en
Inventor
David Daf
Original Assignee
Taigen Bioscience Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Taigen Bioscience CorpfiledCriticalTaigen Bioscience Corp
Priority to TW094103773ApriorityCriticalpatent/TWI253957B/en
Priority to JP2006015460Aprioritypatent/JP4459172B2/en
Priority to DE102006003777Aprioritypatent/DE102006003777B4/en
Priority to KR1020060010679Aprioritypatent/KR100830283B1/en
Priority to GB0602249Aprioritypatent/GB2422795B/en
Priority to FR0601045Aprioritypatent/FR2882943B1/en
Application grantedgrantedCritical
Publication of TWI253957BpublicationCriticalpatent/TWI253957B/en
Publication of TW200628228ApublicationCriticalpatent/TW200628228A/en

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Abstract

An apparatus for processing a biochemical sample is applicable in cleaning, separation or purification of biochemical molecules, e.g. DNA, RNA or proteins, etc., in biological laboratories, and includes: at least a semi-permeable membrane through pipe, at least a transfer pipe, and a pneumatic box. These components are assembled together and are joined together by using an airtight material, e.g. an airtight elastic ring. Thus, when the effective air pressure of the semi-permeable membrane through pipe is larger than the air pressure of the pneumatic box, the invented device displays an airtight status. If at this time a liquid specimen is loaded in the semi-permeable membrane through pipe, the pneumatic pressure pushes the liquid in the pipe to go through the special semi-permeable membrane and flow out of the pipe in order to achieve its purpose of use. The invented device is easy in assembly/disassembly compared to a conventional device.

Description

Translated fromChinese

!253957 九、發明說明: 【發明所屬之技術領域】 本發明係關於一種處理生化檢體之裝置 【先前技術】 在生物相關實驗室,f使料透膜通管清洗、分離或純 化生化分子,如DNA、驗或蛋白質。一般的半透膜通管 =為圓柱管,其底部放置—片或多片具特殊用途之半透膜, 官内可裝入液體,並施以適當的作用力於管内液體上,推 動液體通過半透膜流出管外而達到其助目的。 一般使用的作用力係包括離心力或氣体壓力,當使用離 心力操作半透膜通管時,通常先將半透膜管放人—液體收 集管,其次將液體檢體裝入半透膜管,再將其整個放入於 離心機之轉盤孔内,而後再開動離心機,以利用高速旋轉 :離心力,使液體通過半透膜流出管外而進入液體收集 官。如同以上—次動作之描述’當需連續多次操作時,特 別是連續清洗半透膜通管時,常伴隨很多重複多次的操作 動作’對操作者有較大的負擔。而當使用氣体麼力時,可 將多支半透膜通管分別插入同一個麼力盒,再施以正或負 氣体屋力’使液體通過半透膜流出通管而直接進入液體收 集瓶,對於多檢体或需連續多次之操作較方便。 更近距離觀看半透膜通管31(請參考圖1),其在幾何學上 的設計,通常可分成三部份··上頸部311、中間管體部312 :下:部3"。上頸部311直徑較管體大,有的連蓋子314; 間官體部份312主要用來裳液體檢體,其内底部有一片或 97623.doc 1253957 夕片具特殊用途之半透膜(圖未顯示);下尖部3丨3的設計則 有的有’有的無。 又如圖1所示,半透膜通管31和壓力盒13之連接方式,傳 統上都彳木取緊密插入式,亦即將半透膜通管3丨之下尖部B 3 直接或藉由插入式轉接管33插入壓力盒孔34。插入式轉接 管33主要用來避免半透膜通管31直接插入壓力盒孔μ,因 壓力盒孔34會重複被不同檢體之半透膜通管使用,而造成 檢體與檢體之間的交又污染。插入式轉接管33可以是即丟 式,清洗後重複使用’使用插人式轉接管33時,將半透膜 通g 31之下尖部313插入插入式轉接管%,然後再整個插入 廢力盒孔34’因㈣成半透料管儿插人式轉接管33•壓力 盒32之結構。很多應用都會用到插人式轉接扣,尤其一 些敏感怕檢體間之交叉污染的實驗,如純化核酸用於pcR 反應。這種連接方式必須緊密以避免漏氣,常須用手指抓 緊半透膜通管31及插人式轉接f 33,以便”,造成手指 疼痛,有時因插的太緊又必須費力拔出,又有時需層層相 插亦不易操作,因而在進行半透膜通管之多量檢體操作時 極為不便。 有鑑於此,申請人遂提供一 半透膜通管、轉接管及壓力盒 作者可輕鬆快速操作此裝置。 【發明内容】 種處理生化檢体之裝置,使 間以鬆配合互相容置,使操 種處理生化檢體之裝 轉接管及一氣壓盒, 為達成上述目的,本發明係提供一 置,其包含至少一半透膜通管、至少一 97623.doc 1253957 均以鬆配合互相容置,並 相互接觸位置’俾當該半透膜通Γ之Γ厂氣密彈性圈置於其 盒時,本裝置呈現-氣密狀態 液體檢体,此氣体壓力可推動^ 一透料官内裝有 半透膜而产出其外 _ s内液体通過其特殊功能之 处朕向級出官外,以達 包含-内目的。鮮透膜通管係 、邛&域及一底部區域。 内部界定出-第一容置空間 “透膜通官之253957 IX. Description of the Invention: [Technical Field] The present invention relates to a device for processing a biochemical sample [Prior Art] In a biologically relevant laboratory, the material is permeated through a membrane to clean, separate or purify biochemical molecules. Such as DNA, test or protein. The general semi-permeable membrane tube is a cylindrical tube, and the bottom is placed with a piece or a plurality of semi-permeable membranes for special purposes. The liquid can be filled into the body and the appropriate force is applied to the liquid in the tube to push the liquid through. The semi-permeable membrane flows out of the tube to achieve its purpose. The commonly used force includes centrifugal force or gas pressure. When using a centrifugal force to operate a semipermeable membrane tube, the semipermeable membrane tube is usually first placed in a liquid collection tube, and then the liquid sample is placed in a semipermeable membrane tube. The whole is placed in the turntable hole of the centrifuge, and then the centrifuge is started to take advantage of the high-speed rotation: centrifugal force to allow the liquid to flow out of the tube through the semi-permeable membrane and enter the liquid collection officer. As described above, the description of the sub-operations requires a large burden on the operator when the operation of the semi-permeable membrane is continuously cleaned, in particular, with many repeated operation operations. When using a gas force, a plurality of semi-permeable membrane tubes can be respectively inserted into the same force box, and then a positive or negative gas house force can be applied to allow the liquid to flow out through the semi-permeable membrane and directly into the liquid collection bottle. It is convenient for multiple samples or for multiple consecutive operations. The semipermeable membrane tube 31 (see Fig. 1) is viewed closer, and its geometric design is generally divided into three parts: an upper neck 311, an intermediate tube portion 312: lower: a portion 3". The upper neck 311 has a larger diameter than the tubular body, and some have a cover 314; the intermediate body portion 312 is mainly used for a liquid sample, and has a piece at the bottom or 97623.doc 1253957. The special film is semi-permeable ( The figure is not shown); the design of the lower tip 3丨3 has some 'none. As shown in FIG. 1, the connection mode of the semi-permeable membrane through-tube 31 and the pressure box 13 is conventionally a close-inserted type of eucalyptus, that is, the tip B 3 of the semi-permeable membrane tube 3 is directly or by The plug-in transfer tube 33 is inserted into the pressure cell hole 34. The plug-in transfer tube 33 is mainly used to prevent the semi-permeable membrane tube 31 from being directly inserted into the pressure box hole μ, because the pressure box hole 34 is repeatedly used by the semi-permeable membrane tube of different specimens, resulting in the specimen and the specimen. The traffic is polluted. The plug-in transfer tube 33 can be a drop-on type, and can be reused after cleaning. When the plug-in transfer tube 33 is used, the semi-permeable membrane is inserted into the plug-in transfer tube by the tip 313 of the g 31, and then the whole The insertion of the waste box hole 34' is configured by the (four) semi-transparent tube insertion type transfer tube 33 and the pressure box 32. Many applications use plug-in adapters, especially those that are sensitive to cross-contamination between specimens, such as purified nucleic acids for pcR reactions. This connection must be tight to avoid air leaks. It is often necessary to grasp the semi-permeable membrane tube 31 and the insertion adapter f 33 with your fingers, so as to cause finger pain, sometimes it is too difficult to pull out due to too tight insertion. Moreover, it is sometimes difficult to operate by layer-by-layer insertion, which is extremely inconvenient when performing a large amount of sample operation of the semi-permeable membrane. In view of this, the applicant provides half of the membrane through-tube, the transfer tube and the pressure box. The author can operate the device easily and quickly. [Summary of the Invention] A device for processing a biochemical sample body, which allows the device to be placed in a loose fit, so that the biochemical sample can be handled and transferred to a gas pressure box, in order to achieve the above purpose. The present invention provides a device comprising at least half of a through-membrane through-tube, at least one 97623.doc 1253957, which are each placed in a loose fit and in contact with each other at a position '俾 气 该 该 该 该 该 该 该 该 该 该 该 该 该 该 该 该 该 该 该When the ring is placed in the box, the device presents a liquid-tight state liquid sample, and the gas pressure can push the semi-permeable membrane inside the permeable body to produce the liquid outside the _ s liquid through its special function 朕Out of the ranks, to include Purpose: Fresh permeable membrane system, 邛& field and a bottom area. Internally defined - first accommodating space

該頂部區域設有一第 ή\· # v 一片半透膜, 一 幵 自该頂部區域向外延伸之一 凸緣,該底部區域設有一第一通孔。 該轉接管係包含-内部、一頂部區域及— 轉接管界定出一第二 -或。该 工間 6亥頂部區域設有一笫-戸弓 口及自4頂部區域向外 _ 仏伸之弟-凸緣,該底部區域設有 一、。该氣壓盒係包含一盒體之上表面及一各於 該盒體之上表面包含至少-承接槽,自該上表面::延 伸二用以7轉該至少―轉接管,該至少—承接槽之-底部 另叹有一第三通孔和盒體之貯存空間相通。該盒體之内部 界定出一貯存空間。 该半透膜通管係以鬆配合容置於該轉接管之第二容置空 門中且/、頂邛區域之第一凸緣適可抵靠於該轉接管之第 二凸緣上,該第二凸緣外環繞之一氣密件,如一氣密彈性 圈。而該轉接管亦以鬆配合容置於該承接槽中,且其第二 凸緣下適可抵靠於承接槽邊緣之盒體表面,第一通孔和第 二通孔和第三通孔均相通;俾當該半透膜通管之第一容置 空間之氣壓有效大於該氣壓盒之承接槽時,該半透膜通管 97623.doc 1253957 之第-凸緣、該轉接管之第二凸緣與承接 面間均呈現一氣密貼合狀態。 羞體表 本發明亦可於該轉接管底輕域之第二通孔及承接槽底 部之第三通孔間設有-氣密件,.俾當該半透膜通管之卜 之氣㈣效大於該㈣盒之承接槽時,該半透膜 ^官之弟-凸緣與該轉接管之第二凸緣間及該轉接管之底 部區域與該承接槽之底部間均分別呈現一氣密貼合狀能。 此外’本發明亦可將該半透膜通管直接與氣麼盒連接,直 二密件:設於半透膜通管之第-凸緣或承接槽底部之通; 間以達到相同之氣密效果。 為使熟悉該項技術人士更了解本發明之技術特點,並可 據以貫施,兹以下列之實施例配合圖式詳加說明,惟 述僅為用以敘述本發明奋與 八” X月之車乂么只轭例,而非對本發明做任 :形式之限制’凡是在相同發明精神下所作之任何修飾或 、夂更,皆應屬本發明保護之範圍。 一 【實施方式】 圖2係為一習用半透膜通管及使用於本發明之轉接管之 不意圖。半透膜通管u通常為圓柱狀,係包含—内部、一 頂部區域及—底部區域。半透膜通管u之内部係界定出一 弟一容置空間lu,第-容置空間⑴之底部置有至少—片 半透膜11 2。其頂邦p % 域政有一第一開口(圖中未示)及自頂 部區域向外延伸之—第一凸緣113,而底部區域設有一第」 通孔114。轉接營么 要吕丨2係大致為一試管狀,其可以任何人 之材料製成,並包含一内部、一頂部區域及一底部區:。 97623.doc 1253957 轉接管12之内部係界定出-第二容置空間m,其頂部區 域設有-第二開口(圖巾未示)及自該了貝部區域向外延伸之 第二凸緣122’而底部區域設有—第二通孔123。轉接管12 之内徑係大於半透膜通f U中㈣體部份,但小於頂部區 =113之直徑,以使半透膜通管u得以經由轉接管^之 第二開口以鬆配合容置於轉接管12之第二容置空間 内,而半透膜通f U頂部區域之第一凸緣⑴射抵靠於 轉接官12之第二凸緣122上。轉接管12之第二凸緣⑵ 外另可進-步包含一環繞第二凸、緣122之氣密件,其最佳 為-氣密彈性目η’以增強第—凸緣113與第二凸緣122 間之氣密。The top region is provided with a first semi-permeable membrane, and a flange extending outwardly from the top region, the bottom region being provided with a first through hole. The transfer tube includes an inner portion, a top region, and a transfer tube defining a second - or. The top area of the 6-well section is provided with a 笫-戸 bow opening and a 自 之 之 - 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘 凸缘The air pressure box comprises a top surface of a box body and a top surface of the box body comprising at least a receiving groove, and the extending surface is used for extending 7 at least 7 to the transfer tube, the at least one receiving The bottom of the slot is sighed with a third through hole communicating with the storage space of the box. The interior of the box defines a storage space. The semi-permeable membrane through tube is loosely fitted in the second accommodating empty door of the transfer tube and/or the first flange of the top cymbal region is adapted to abut against the second flange of the transfer tube The second flange is surrounded by a gas-tight member such as an airtight elastic ring. The transfer tube is also loosely fitted in the receiving groove, and the second flange is adapted to abut against the surface of the box body at the edge of the receiving groove, the first through hole and the second through hole and the third through hole The holes are all connected; when the air pressure of the first accommodating space of the semipermeable membrane tube is effectively greater than the receiving groove of the gas pressure box, the semi-permeable membrane is connected to the first flange of the 97623.doc 1253957, the transfer tube The second flange and the receiving surface each have an airtight fit state. The invention can also be provided with a gas-tight member between the second through hole of the light pipe bottom of the transfer pipe and the third through hole at the bottom of the receiving groove, and the gas of the semipermeable membrane is passed through the pipe (4) When the effect is greater than the receiving groove of the (4) box, the semi-permeable membrane and the second flange of the transfer tube and the bottom portion of the transfer tube and the bottom of the receiving groove are respectively presented An airtight fit can. In addition, the present invention can also directly connect the semipermeable membrane tube to the gas box, and the straight two-density member is disposed at the first flange of the semipermeable membrane tube or the bottom of the receiving groove; effect. In order to make those skilled in the art more aware of the technical features of the present invention, and to be consistently described, the following embodiments are described in detail with reference to the drawings, but are merely used to describe the present invention. 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 It is a conventional semi-permeable membrane tube and is not intended for use in the transfer tube of the present invention. The semi-permeable membrane tube u is generally cylindrical, and includes an inner portion, a top portion, and a bottom portion. The inner portion of the tube u defines a room-accommodating space lu, and the bottom of the first-accommodating space (1) is provided with at least a semi-transparent film 11 2 . Its top state p % domain has a first opening (not shown in the figure) And a first flange 113 extending outward from the top region and a first through hole 114 in the bottom region. Transfer Camp The Lu Wei 2 Series is roughly a test tube, which can be made of any person's material and includes an interior, a top area and a bottom area: 97623.doc 1253957 The interior of the transfer tube 12 defines a second accommodating space m, the top portion of which is provided with a second opening (not shown) and a second convex extending outward from the shell portion The edge 122' is provided with a second through hole 123. The inner diameter of the transfer tube 12 is larger than the (four) body portion of the semipermeable membrane, but smaller than the diameter of the top portion = 113, so that the semipermeable membrane tube u can be loosened through the second opening of the transfer tube The fitting is placed in the second receiving space of the transfer tube 12, and the first flange (1) of the semi-permeable membrane passing through the top region of the fU is directed against the second flange 122 of the adapter 12. The second flange (2) of the transfer tube 12 further includes a hermetic member surrounding the second protrusion and the edge 122, which is preferably a hermetic elastic η' to enhance the first flange 113 and the second The air gap between the flanges 122.

現蒼考目3及目4,其係顯示依據本發明之處理生化檢體 之裝置10之立體圖及其剖面圖。處理生化檢體裝置1〇係 包含至少一半透膜通管π、至少一轉接管12及一氣壓盒 14。氣壓盒14係包含一盒體之上表面141及一盒體142。 盒體142之内部界定出一貯存空間145(參考圖句,盒體之 上表面141係包含至少一承接槽143,自上表面141向下延 伸,用以承接至少一轉接管12,承接槽143之一底部另設 有一第三通孔144和盒體142之貯存空間丨45相通。承接 槽143之孔徑係略大於轉接管12中間管體部份,但小於轉 接管12第二凸緣122之直徑,使半透膜通管Η可於容置 於轉接管1 2後以鬆配合容置於承接槽丨43中,而第二凸緣 122下適可抵靠於承接槽143邊緣之盒體上表面141,此時 第一通孔114、第二通孔123及第三通孔144均相通。轉接 97623.doc !253957 吕12之第二凸緣122外包含一環繞第二凸緣I]]之氣密 件,其最佳為一氣密彈性圈13。因而當半透膜通管u之第 谷置空間111之氣壓有效大於氣壓盒14之承接槽工43 時,半透膜通管11之第一凸緣113、轉接管12之第二凸緣 122與承接槽143邊緣之盒體表面141間均呈現一氣密貼合 狀態,此時氣体壓力可作用在管内液體,使其流過半透膜 (翏考圖4)流到管外而達到操作目的。轉接管12除以上串 _ 鬆配合容置及氣密效果外,亦可避免氣壓盒14之承接槽 I43與半透膜通管11直接接觸及重複被不同檢體之半透膜 通言11使用而造成檢體與檢體之間的交又污染。 同樣地,當轉接管12,以鬆配合部分容置於承接槽143, 時,半透膜通管11,、轉接管12,及氣壓盒14,間之氣密方式, 亦如圖5及圖6所示。一氣密彈性圈125可設於轉接管12, 之第一通孔123’及承接槽143’之第三通孔144,之間,因而當 半透膜通管11’之第一容置空間m,之氣壓有效大於盒體14, _ 之承接槽143’時,半透膜通管π’之第一凸緣J13,與轉接管 12’之第二凸緣122,間及轉接管12,之底部區域、氣密彈性圈 125及承接槽143之底部間均分別呈現一氣密貼合狀態。轉 接官12’之第二凸緣122,外亦可進一步包含一環繞第二凸緣 122’之彈性圈13’,以增強第一凸緣113,與第二凸緣122,間 ' 之氣密。又如圖7及圖8所示,本發明亦可將半透膜通管 11、11直接與氣壓盒14”、14’’’連接,其氣密彈性圈13,,、 氣岔彈性圈125可設於半透膜通管11 ”、1丨”,之第一凸緣1 j 3,, 或半透膜通管11”,之第一通孔U4,”與承接槽143",底部之第 97623.doc 10 1253957 二通孔144”’間,俾當該半透膜通管 _ I弟一容置空間1 Π,, 之氣壓有效大於該氣壓盒14”之承接枰 ^ θ 3才,該半透膜通 管U”之第-凸緣⑴,,與承接槽143”邊緣之盒體表面間呈現 一氣密貼合狀態,或當該半透膜通管1 & 1灸弟一容置空間 111”’之氣壓有效大於該氣壓盒14”,之貯在*門, <灯仔工間145”,時,該半 透膜通管11",之底部區域與該承接槽143,”之底部間呈現一 氣密貼合狀態。References 3 and 4 show a perspective view of a device 10 for processing a biochemical sample according to the present invention and a cross-sectional view thereof. The biochemical sample device 1 comprises at least half of the membrane through tube π, at least one transfer tube 12 and a gas pressure box 14. The air box 14 includes a box upper surface 141 and a box 142. The interior of the casing 142 defines a storage space 145 (refer to the figure, the upper surface 141 of the casing includes at least one receiving groove 143 extending downward from the upper surface 141 for receiving at least one transfer pipe 12, the receiving groove A third through hole 144 is connected to the storage space 丨45 of the casing 142 at one of the bottoms of the 143. The aperture of the receiving groove 143 is slightly larger than the intermediate pipe portion of the transfer pipe 12, but smaller than the second convex portion of the transfer pipe 12. The diameter of the edge 122 allows the semipermeable membrane tube to be received in the receiving groove 43 after being placed in the transfer tube 12, and the second flange 122 is adapted to abut against the receiving groove 143. The upper surface of the box body 141, at this time, the first through hole 114, the second through hole 123 and the third through hole 144 are all connected. The adapter 9723.doc !253957 The airtight member of the second flange I] is preferably an airtight elastic ring 13. Therefore, when the air pressure of the first valley space 111 of the semipermeable membrane tube u is effectively greater than the receiving tank 43 of the air pressure box 14, the semi-transparent The first flange 113 of the membrane tube 11, the second flange 122 of the transfer tube 12 and the box surface 141 of the edge of the receiving groove 143 are airtightly fitted. State, at this time, the gas pressure can act on the liquid in the tube, and it flows through the semi-permeable membrane (refer to Figure 4) and flows out of the tube to achieve the operation purpose. The transfer tube 12 is in addition to the above-mentioned string _ loose fit and airtight effect It is also possible to prevent the receiving groove I43 of the air box 14 from directly contacting the semipermeable membrane through tube 11 and repeating the use of the semipermeable membrane of the different specimens to cause contamination between the sample and the specimen. When the transfer tube 12 is accommodated in the receiving groove 143 with the loose fitting portion, the semi-permeable membrane through tube 11, the transfer tube 12, and the pneumatic box 14 are also airtight, as shown in FIG. 5 and As shown in Fig. 6. A hermetic elastic ring 125 can be disposed between the first through hole 123' of the transfer tube 12 and the third through hole 144 of the receiving groove 143', and thus the semipermeable membrane through tube 11' When a space m is accommodated, the air pressure is effectively greater than the receiving groove 143' of the casing 14, _, the first flange J13 of the semipermeable membrane pipe π', and the second flange 122 of the transfer pipe 12', And the transfer tube 12, the bottom region, the airtight elastic ring 125 and the bottom of the receiving groove 143 respectively exhibit an airtight fit state. The second flange 122 of the transfer officer 12' can be further inserted into the outer flange 12 The elastic ring 13' surrounding the second flange 122' is included to enhance the airtightness of the first flange 113 and the second flange 122. As shown in FIGS. 7 and 8, the present invention may also The semipermeable membrane tubes 11, 11 are directly connected to the pneumatic boxes 14", 14"", and the airtight elastic ring 13, the pneumatic elastic ring 125 can be disposed in the semipermeable membrane through tubes 11", 1"" , the first flange 1 j 3, or the semi-permeable membrane through tube 11", the first through hole U4, "with the receiving groove 143 ", the bottom of the 97623.doc 10 1253957 two through hole 144"',俾 When the semi-permeable membrane tube _ I brother accommodates the space 1 Π, the air pressure is effectively greater than the pressure 14 θ 3 of the air box 14 ′′, the semi-permeable membrane tube U ′ the first flange (1) , a gas-tight fit state is formed between the surface of the box body at the edge of the receiving groove 143", or the air pressure of the semi-permeable film through tube 1 & 1 moxibustion room 111"' is effectively greater than the air pressure box 14" When it is stored in the * door, <lighting room 145", the semi-permeable membrane through tube 11", the bottom portion and the receiving groove 143," the bottom of the bottom of the air-tight state.

由如以上可知’本發明提供—種活動式、易裝置及易拆 卸之處理生化檢體之裝置,並改善習用裝置之諸多缺點。 因半透膜通管及轉接管具有易放入及易取出之優點,'亦可 應用於自動化操作以機器手臂移動半透膜通管。 【圖式簡單說明】 圖1係顯示一習用處理生化檢體裝置之示意圖; 圖2係顯示一習用半透膜通管及使用於本發明之轉接管 之不意圖; 圖3係顯示依據本發明之處理生化檢體裝置之立體圖; 圖4係為圖3之剖面圖; 圖5係顯示處理生化檢體之裝置另一實施例之立體圖; 圖6係為圖5之剖面圖; 圖7係顯示處理生化檢體之裝置另一實施例之立體圖; 圖8係為圖7之剖面圖; 圖9係顯示處理生化檢體之裝置另一實施例之立體圖;及 圖10係為圖9之剖面圖。 97623.doc 11 1253957 【主要元件符號說明】As can be seen from the above, the present invention provides a mobile, easy-to-disassemble and easy-to-disassemble apparatus for processing biochemical samples, and improves many disadvantages of conventional devices. Because the semi-permeable membrane tube and the transfer tube have the advantages of easy insertion and easy removal, 'can also be applied to the automatic operation to move the semi-permeable membrane through the robot arm. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a conventional biochemical sample device; FIG. 2 is a schematic view showing a conventional semipermeable membrane tube and an adapter tube used in the present invention; 3 is a perspective view of the biochemical sample device; FIG. 4 is a cross-sectional view of another embodiment of the device for processing a biochemical sample; FIG. 6 is a cross-sectional view of FIG. 5; FIG. 8 is a cross-sectional view showing another embodiment of the apparatus for processing a biochemical specimen; FIG. 9 is a perspective view showing another embodiment of the apparatus for processing a biochemical specimen; Figure. 97623.doc 11 1253957 [Main component symbol description]

10 處理生化檢體裝置 10, 處理生化檢體裝置 10,, 處理生化檢體裝置 10,,, 處理生化檢體裝置 11 半透膜通管 11, 半透膜通管 11,’ 半透膜通管 11,’, 半透膜通管 12 轉接管 12, 轉接管 13 氣密彈性圈 13, 氣密彈性圈 13,, 氣密彈性圈 14 氣壓盒 14, 氣壓盒 14,, 氣壓盒 14m 氣壓盒 31 半透膜通管 32 氣壓盒 33 轉接管 34 壓力盒孔 111 第一容置空間 111, 第一容置空間 97623.doc -12- 125395710 processing biochemical sample device 10, processing biochemical sample device 10, processing biochemical sample device 10, processing biochemical sample device 11 semipermeable membrane through tube 11, semipermeable membrane through tube 11, 'semipermeable membrane through Tube 11, ', semi-permeable membrane tube 12 adapter tube 12, adapter tube 13 hermetic elastic ring 13, hermetic elastic ring 13, airtight elastic ring 14 pneumatic box 14, pneumatic box 14, pneumatic box 14m Pneumatic box 31 semi-permeable membrane through pipe 32 air pressure box 33 transfer tube 34 pressure box hole 111 first accommodation space 111, first accommodation space 97623.doc -12- 1253957

111” 第一容置空間 111’" 第一容置空間 112 半透膜 113 第一凸緣 113, 第一凸緣 113" 第一凸緣 114 第一通孔 114’,, 第一通孔 121 第二容置空間 122 第二凸緣 122, 第二凸緣 123 第二通孔 123, 第二通孔 125 氣密彈性圈 125’,, 氣密彈性圈 141 上表面 142 盒體 143 承接槽 143, 承接槽 143,, 承接槽 143,,, 承接槽 144 第三通孔 144’ 第三通孔 144… 第二通孔 145 貯存空間 97623.doc 1253957111" first accommodating space 111'" first accommodating space 112 semi-permeable membrane 113 first flange 113, first flange 113" first flange 114 first through hole 114', first through hole 121 second accommodating space 122 second flange 122, second flange 123 second through hole 123, second through hole 125 airtight elastic ring 125', airtight elastic ring 141 upper surface 142 case 143 receiving groove 143, receiving groove 143, receiving groove 143,, receiving groove 144, third through hole 144', third through hole 144... second through hole 145 storage space 97623.doc 1253957

145”’ 貯存空間 311 上頸部 312 中間管體部 313 下尖部 314 蓋子 97623.doc -14145”’ storage space 311 upper neck 312 middle tube body 313 lower tip 314 cover 97623.doc -14

Claims (1)

Translated fromChinese
1253957 十、申請專利範圍: 1. 一種處理生化檢體之裝置,包含: 至少一半透膜通管,包含·· 该容置空間底 内部,界定出一第一容置空間 置有至少一片半透膜; 口及自該頂部區域向 5又, 外延伸之一第一凸緣;及1253957 X. Patent application scope: 1. A device for processing biochemical samples, comprising: at least half of a membrane through-tube, comprising: · inside the bottom of the accommodating space, defining a first accommodating space with at least one semi-transparent a membrane; a mouth and a first flange extending outward from the top region; and一底部區域,設有一第一通孔; 至少一轉接管,包含: 一内部,界定出一第二容置空間; 一頂部區域,設有一第-M 从 弟—開口及自該頂部區域向 外延伸之第二凸緣;及 一底部區域,設有一第二通孔; 一氣壓盒,包含·· 益體,其内部界定出一貯存空間;及 -盒體之上表面,包含至少一承接槽,自該上表 面向下延伸’用以承接該至少—轉接管,該至少一 承接槽之—底部另設有_第三通孔與該盒體之貯存 空間相通; *該半透膜通管係以鬆配合容置於該轉接管之第二容置 > 中其頂部區域之第一凸緣適可抵靠於該轉接管之 =二凸緣上’且該第二凸緣外環繞-氣密件,而該轉接 g f以秦配合容置於該承接槽中,且其n緣下適可 一 #、承接槽邊緣之盒體表面,第一通孔和第二通孔和 97623.doc 1253957 第三通孔均相通;俾當該半透膜通管之第一容置空間之 氣壓有效大於該氣壓盒之承接槽時,該半透膜通管之第 一凸緣與該轉接管之第二凸緣間,及該轉接管之第二凸 緣與承接槽邊緣之盒體表面間均呈現一氣密貼合狀態。 2. 一種處理生化檢體之裝置,包含: 至少一半透膜通管,包含: 一内部,界定出一第一容置空間,該容置空間底 置有至少一片半透膜; 一頂部區域,設有一第一開口及自該頂部區域向 外延伸之一第一凸緣;及 一底部區域,設有一第一通孔; 至少一轉接管,包含: 一内部,界定出一第二容置空間; 一頂部區域,設有一第二開口及自該頂部區域向 外延伸之第二凸緣;及 一底部區域,設有一第二通孔; 一氣壓盒,包含: 一盒體,其内部界定出一貯存空間;及 一盒體之上表面,包含至少一承接槽,自該上表 面向下延伸,用以承接該至少一轉接管,該至少一 承接槽之一底部另設有一第三通孔與該盒體之貯存 空間相通, 該半透膜通管係以鬆配合容置於該轉接管之第二容置 空間中,且其頂部區域之第一凸緣適可抵靠於該轉接管 97623.doc -2- 1253957 弟二凸緣上,且該第二凸緣外環繞一氣密件,而該轉 接管亦以鬆配合部分容置於該承接槽中,該底部第二通 孔可抵靠於承接槽底部之第三通孔邊緣,其間設有一氣 抢件,第一通孔和第二通孔和第三通孔均相通;俾當該 半透膜通官之第一容置空間之氣壓有效大於該氣壓盒之 承接槽時,該半透膜通管之第一凸緣與該轉接管之第二 凸緣間及該轉接管之底部區域與該承接槽之底部間均分 別呈現一氣密貼合狀態。 1如請求項1或2之處理生化檢體之裝置’其中該氣密件係為 一氣密彈性圈。 4· 種用於請求項1或2之處理生化檢體之裝置之轉接管, 包含: 一内部,界定出一容置空間; —頂部區域,設有-開口及自該頂部區域向外延伸 之凸緣’該凸緣環繞一氣密彈性圈;及 一底部區域,設有一通孔。 一種處理生化檢體之裝置,包含: 至少一半透膜通管,包含: 4容置空間底 一内部,界定出一第一容置空間 置有至少一片半透膜; 一頂部區域,設有一第 外延伸之一第一凸緣;及 一開口及自該 丁貝部區域向 一底部區域,設有一第一通孔; 一氣壓盒,包含: 97623.doc 1253957 一盒體,其内部界定屮 日a十〜 丨疋出一貝丁存空間;及 一盒體之上表面,包含 至/ 一承接槽,自該上表 面向下延伸,用以承接 .关μ至/ 一轉接管,該至少_ 承接槽之一底部另設有_ 空間相通; 通孔與該盒體之貯存 該+透膜通管以鬆配合容置於該承接槽中,且— 凸緣下適可抵靠於承接槽邊 ” 备它政〜 水<|體表面,其間設有一 瑕^件,弟一通孔和第二通孔 令梦一〜职 早田5亥+透膜通管 之弟谷置空間之氣壓有效大於兮洛颅人 兮本读脸、ρ > ’政大於忒乳壓盒之承接槽時, μ半透膜通管之第一凸绫愈 〜 ㈣承接槽邊緣之盒體表面間呈 現一乳岔貼合狀態。 6 一種處理生化檢體之裝置,包含·· 至少一半透膜通管,包含: 一内部,界定出一第一容 罟古。, *置工間,該容置空間底 置有至少一片半透膜; 夕^頂部區域,設有—第—開π及自該頂部區域向 卜l伸之一第一凸緣;及 一底部區域,設有一第一通孔; 一氣壓盒,包含·· —盒體,其内部界定出一貯存空間;及 ~盒體之上表面,包含至少_承接槽,自該上表 面向下延伸,用以承接該至少一轉接管,該至少J 接槽之一底部另設有一第二通 空間相通; …玄皿體之貯存 97623.doc 1253957 該半透膜通營在 产 ’、氣、配合部分容置於該承接槽中,立 、 弟通孔可抵靠於承接槽底部之第二通孔, 並與第二通孔相通,盆 < 、一 十 /、間5又有一氣密件;俾當該半透膜 通吕之第a置空間之氣壓有效大於該氣壓盒之貯存空 間時,該半透膜通管之底部區域與該承接槽之底部^ 現一氣密貼合狀態。a bottom portion is provided with a first through hole; at least one transfer tube includes: an inner portion defining a second receiving space; and a top portion having a first-M slave-opening and from the top portion a second flange extending outwardly; and a bottom portion provided with a second through hole; a pneumatic box containing a body defining a storage space therein; and - an upper surface of the box containing at least one receiving space a slot extending downward from the upper surface to receive the at least one of the transfer tubes, the bottom of the at least one receiving groove is further provided with a third through hole communicating with a storage space of the box; * the semipermeable membrane The first pipe flange of the top portion of the adapter tube is adapted to be placed in the second receiving portion of the adapter tube with a loose fit; and the second flange is adapted to abut against the second flange of the adapter tube and the second protrusion The outer edge surrounds the airtight member, and the transfer gf is placed in the receiving groove with the Qin fitting, and the n-edge is suitable for one, the surface of the box receiving the edge of the groove, the first through hole and the second through hole And the third through hole of the 97623.doc 1253957 is connected; when the first accommodating space of the semipermeable membrane is passed When the air pressure is effectively greater than the receiving groove of the air box, the first flange of the semipermeable membrane tube and the second flange of the transfer tube, and the second flange of the transfer tube and the box at the edge of the receiving groove The air surface is in a state of airtight fit. 2. A device for processing a biochemical sample, comprising: at least half of a membrane through-tube, comprising: an interior defining a first accommodating space, the accommodating space having at least one semi-permeable membrane at the bottom; a top region, a first opening and a first flange extending outward from the top region; and a bottom portion, a first through hole; at least one transfer tube comprising: an interior defining a second receiving a top portion having a second opening and a second flange extending outwardly from the top portion; and a bottom portion having a second through hole; a pneumatic box comprising: a casing defining an interior thereof And a storage space; and a top surface of the casing, comprising at least one receiving groove extending downward from the upper surface for receiving the at least one transfer tube, and a third portion of the at least one receiving groove is further provided with a third portion The through hole communicates with the storage space of the box body, and the semipermeable membrane tube is loosely fitted in the second accommodating space of the transfer tube, and the first flange of the top region is adapted to abut The transfer tube 97623.doc -2- 12 53957 is a flange of the second flange, and the second flange is surrounded by a gas-tight member, and the adapter tube is also received in the receiving groove by a loose fitting portion, and the bottom second through hole can abut against the bottom of the receiving groove a third through hole edge with a gas grabbing member therebetween, wherein the first through hole and the second through hole and the third through hole are in communication; and when the semipermeable membrane passes through the first receiving space, the air pressure is effectively greater than the air pressure When the receiving groove of the box, the first flange of the semipermeable membrane tube and the second flange of the transfer tube and the bottom portion of the transfer tube and the bottom of the receiving groove respectively exhibit an airtight fit status. A device for processing a biochemical sample according to claim 1 or 2, wherein the airtight member is an airtight elastic ring. 4. An adapter tube for a device for processing a biochemical specimen of claim 1 or 2, comprising: an interior defining an accommodation space; a top region having an opening and extending outwardly from the top region The flange 'the flange surrounds an airtight elastic ring; and a bottom portion is provided with a through hole. The invention relates to a device for processing a biochemical sample, comprising: at least half of a membrane through-tube, comprising: 4 a receiving space bottom and an inner portion, defining a first receiving space with at least one semi-permeable membrane; a top region having a first a first flange extending outwardly; and an opening and a first through hole from the peripheral portion to the bottom portion; a pneumatic box comprising: 97623.doc 1253957 a box body, the interior of which defines the next day a ten ~ 丨疋 一 贝 贝 ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; _ One of the bottoms of the receiving groove is provided with _ spatial communication; the through hole and the storage box of the box are loosely fitted in the receiving groove, and the bottom of the flange is adapted to abut against the receiving groove Side" Prepare it~ Water <| Body surface, with a piece of it in between, the younger one through hole and the second through hole make the dream one ~ the job early field 5 Hai + the membrane through the tube of the valley space effective兮洛颅人兮本读脸,ρ > '政大忒忒乳压盒When receiving the groove, the first convex ridge of the μ semi-permeable membrane is made to a lower pressure. (4) A nipple conforming state is formed between the surfaces of the casings at the edge of the receiving groove. 6 A device for processing a biochemical sample, comprising at least half of the permeable membrane The pipe, comprising: an interior, defines a first volume of the ancient.. * In the work space, the accommodating space is provided with at least one semi-permeable membrane at the bottom; the oh ^ top area is provided with - first - open π and The top region extends to a first flange; and a bottom portion is provided with a first through hole; a pneumatic box containing a box body defining a storage space therein; and The surface includes at least a receiving groove extending downward from the upper surface for receiving the at least one transfer tube, and at least one of the at least one J-shaped groove is provided with a second through space at the bottom; .doc 1253957 The semi-permeable membrane is placed in the receiving groove in the production, gas and mating parts, and the vertical through hole can abut the second through hole at the bottom of the receiving groove and communicate with the second through hole. , basin <, one ten /, between 5 and one airtight; When the semipermeable membrane through a first Lu of the effective pressure is greater than the space between the cartridge storage space of the gas pressure when the bottom region of the semi-permeable and the bottom of the receiving groove ^ Now a hermetically bonded state.如請求項5或6之處理生化檢體之裝置,其中該氣密件係為 一氣密彈性圈。A device for processing a biochemical sample according to claim 5 or 6, wherein the airtight member is an airtight elastic ring.97623.doc97623.doc
TW094103773A2005-02-042005-02-04Apparatus for processing biochemical sampleTWI253957B (en)

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TW094103773ATWI253957B (en)2005-02-042005-02-04Apparatus for processing biochemical sample
JP2006015460AJP4459172B2 (en)2005-02-042006-01-24 Apparatus for processing biological samples
DE102006003777ADE102006003777B4 (en)2005-02-042006-01-25 Device for processing biological samples and use of a vacuum connection column or a vacuum connection tube
KR1020060010679AKR100830283B1 (en)2005-02-042006-02-03Apparatus for processing biological sample
GB0602249AGB2422795B (en)2005-02-042006-02-06Apparatus for processing biological sample
FR0601045AFR2882943B1 (en)2005-02-042006-02-06 APPARATUS FOR PROCESSING BIOLOGICAL SAMPLES.

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GB2422795A (en)2006-08-09
GB2422795B (en)2010-12-08
DE102006003777B4 (en)2010-06-10
GB0602249D0 (en)2006-03-15
FR2882943A1 (en)2006-09-15
TW200628228A (en)2006-08-16
KR100830283B1 (en)2008-05-16
DE102006003777A1 (en)2007-01-18
JP2006212028A (en)2006-08-17
KR20060089668A (en)2006-08-09
FR2882943B1 (en)2009-10-30

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