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TW200512459A - Global analysis for protein expression kit and protein qualitative and quantitative method thereof - Google Patents

Global analysis for protein expression kit and protein qualitative and quantitative method thereof

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Publication number
TW200512459A
TW200512459ATW092126189ATW92126189ATW200512459ATW 200512459 ATW200512459 ATW 200512459ATW 092126189 ATW092126189 ATW 092126189ATW 92126189 ATW92126189 ATW 92126189ATW 200512459 ATW200512459 ATW 200512459A
Authority
TW
Taiwan
Prior art keywords
protein
quantitative method
qualitative
global analysis
protein expression
Prior art date
Application number
TW092126189A
Other languages
Chinese (zh)
Other versions
TWI328117B (en
Inventor
Shu-Hui Chen
Rui-Liang Xu
Sheng-Yu Huang
Original Assignee
Univ Nat Cheng Kung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nat Cheng KungfiledCriticalUniv Nat Cheng Kung
Priority to TW092126189ApriorityCriticalpatent/TW200512459A/en
Priority to US10/909,294prioritypatent/US7338806B2/en
Publication of TW200512459ApublicationCriticalpatent/TW200512459A/en
Application grantedgrantedCritical
Publication of TWI328117BpublicationCriticalpatent/TWI328117B/zh

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Abstract

The present invention provides a global analysis for protein expression (GAPE) kit and protein qualitative and quantitative method thereof, which mainly employ an isotope-coded affinity tag (ICAT) reagent, target at normal cell or a protein peptide in an abnormal cell digested through an enzyme to carry out a chemical modification. Further combining with a spectrometry technique, identify a protein sequence through a serially connected spectrometry and precisely quantify the protein performance according to the rise and fall of the signal.
TW092126189A2003-09-232003-09-23Global analysis for protein expression kit and protein qualitative and quantitative method thereofTW200512459A (en)

Priority Applications (2)

Application NumberPriority DateFiling DateTitle
TW092126189ATW200512459A (en)2003-09-232003-09-23Global analysis for protein expression kit and protein qualitative and quantitative method thereof
US10/909,294US7338806B2 (en)2003-09-232004-08-03Reagent kit of global analysis for protein expression and method for qualitative and quantitative proteomic analysis using the same

Applications Claiming Priority (1)

Application NumberPriority DateFiling DateTitle
TW092126189ATW200512459A (en)2003-09-232003-09-23Global analysis for protein expression kit and protein qualitative and quantitative method thereof

Publications (2)

Publication NumberPublication Date
TW200512459Atrue TW200512459A (en)2005-04-01
TWI328117B TWI328117B (en)2010-08-01

Family

ID=34311583

Family Applications (1)

Application NumberTitlePriority DateFiling Date
TW092126189ATW200512459A (en)2003-09-232003-09-23Global analysis for protein expression kit and protein qualitative and quantitative method thereof

Country Status (2)

CountryLink
US (1)US7338806B2 (en)
TW (1)TW200512459A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
CN105785048A (en)*2016-04-152016-07-20同济大学Global protein quantitative analysis method based on light isotope and isobar labeling
CN106990159A (en)*2017-05-042017-07-28同济大学A kind of protein quantitation methods based on complete accurate same weight diethyl mark

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
CA2393726A1 (en)*2002-07-162004-01-16Steven J. LockeQuantitative proteomics via isotopically differentiated derivatization
HRP20100044B1 (en)*2010-01-252016-11-18Institut Ruđer BoškovićProtein identification using mass spectrometry
CA2836114C (en)*2011-05-182016-10-25Rudjer Boskovic InstituteMethod of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonicacid as derivatization reagent
WO2013033713A1 (en)2011-09-022013-03-07Dh Technologies Development Pte. Ltd.System and method for the detection of allergens
CN108088945B (en)*2016-11-212020-11-17中国科学院大连化学物理研究所Absolute quantification method based on dimethylation multiple markers and characteristic fragment ions
CN107796748B (en)*2017-09-282020-06-26上海交通大学Detection method for single-cell mass spectrometry flow cytometry

Cited By (3)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
CN105785048A (en)*2016-04-152016-07-20同济大学Global protein quantitative analysis method based on light isotope and isobar labeling
CN105785048B (en)*2016-04-152017-07-28同济大学Based on the nearly overall Protein quantitative analysis method with heavy label of light isotope
CN106990159A (en)*2017-05-042017-07-28同济大学A kind of protein quantitation methods based on complete accurate same weight diethyl mark

Also Published As

Publication numberPublication date
US20050064515A1 (en)2005-03-24
US7338806B2 (en)2008-03-04
TWI328117B (en)2010-08-01

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MM4AAnnulment or lapse of patent due to non-payment of fees

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