Fig0 34 16 cr cc O 00 O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 November 2018 (08.11.2018) WIPO I PCT omit ° oI0III0101101OHElmoIIII 0IIIIII (10) International Publication Number WO 2018/202894 Al (51) International Patent Classification: C12M 1/34 (2006.01) C12M 3/00 (2006.01) C12M 1/00 (2006.01) (21) International Application Number: PCT/EP2018/061604 (22) International Filing Date: 04 May 2018 (04.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17169597.6 04 May 2017 (04.05.2017) EP 17200210.7 06 November 2017 (06.11.2017) EP (71) Applicants: UNIVERSITAT ZURICH [CH/CH]; Prorek- torat VNW, Ramistr. 71, 8006 Zfirich (CH). NTNU - NORGES TEKNISK-NATURVITENSKAPELIGE UNIVERSITET [NO/NO]; c/o NTNU Technology Trans- fer AS, Sem Selands vei 14, 7491 Trondheim (NO). (72) Inventors: KRIESI, Carlo; Klostergata 44a, 7030 Trond- heim (NO). KURTCUOGLU, Vartan; Reismilhleweg 50, Winterthur 8409 (CH). MARMARAS, Anastasios; Balm- haldenstrasse 12, 8052 Zfirich (CH). STEINERT, Martin; Hogasen 8, 7560 Vikhammer (NO). (74) Agent: SCHULZ, Ben Jesko; Schulz Junghans Patentan- walte PartGmbB, GroBbeerenstraBe 71, 10963 Berlin (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (54) Title: CELL CULTURE DEVICE (57) : The present invention relates to a cell culture device (1) for use with an optical microscope (40), comprising a housing (10) that is configured to be placed onto a stage of an optical microscope (40) in front of an objective (41) of the microscope (40), the housing (10) enclosing an internal space (11) of the housing (10), a removable flow chamber (2) enclosing an internal space (20) for accommodating a cell culture (CC) comprising living biological cells, a heater (3) arranged in the internal space (11) of the housing (10) for heating said fluid medium (M) to be guided through the flow chamber (2), a first flow path (P1) arranged in the internal space (11) of the housing (10) for guiding said fluid medium (M) towards the flow chamber (2) via said heater (3), and a second flow path (P2) arranged in the internal space (11) of the housing (2) for guiding said fluid medium (M) away from the flow chamber (2), and a pump (4) for pumping said fluid medium (M) through the first flow path (P1) into the internal space (20) of the flow chamber (2) and through the second flow path (P2) out of the internal space (20) of the flow chamber (2) when the flow chamber (2) is inserted [Continued on next page] WO 2018/202894 Al MIDEDIMOMOIDEIRDERI001010110111101MOHMEMOIMIE OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the identity of the inventor (Rule 4.17(0) as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) Published: — with international search report (Art. 21(3)) into the internal space (11) of the housing (10).