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SG11201909957TA - Engineered ligase variants - Google Patents

Engineered ligase variants

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Publication number
SG11201909957TA
SG11201909957TASG11201909957TASG11201909957TASG 11201909957T ASG11201909957T ASG 11201909957TASG 11201909957T ASG11201909957T ASG 11201909957TASG 11201909957T ASG11201909957T ASG 11201909957TA
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SG
Singapore
Prior art keywords
california
redwood city
penobscot
international
drive
Prior art date
Application number
Inventor
Mathew Miller
Jonathan Vroom
Nikki Dellas
Donald Baskerville
Sandy Gomes
David Elgart
Judy Viduya
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis IncfiledCriticalCodexis Inc
Publication of SG11201909957TApublicationCriticalpatent/SG11201909957TA/en

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Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 15 November 2018 (15.11.2018) WIPO I PCT ~ill IIIIIIIIII1101DIVIIIIIIIHo o Holommiliu imio oimIE (10) International Publication Number WO 2018/208665 Al (51) International Patent Classification: C12N 9/00 (2006.01) C12N 15/31 (2006.01) C12N 15/09 (2006.01) C12N 15/52 (2006.01) (21) International Application Number: PCT/US2018/031376 (22) International Filing Date: 07 May 2018 (07.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/503,075 08 May 2017 (08.05.2017) US 62/540,734 03 August 2017 (03.08.2017) US 62/587,030 16 November 2017 (16.11.2017) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: MILLER, Mathew„ G.; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). VROOM, Jonathan; 200 Penobscot Drive, Redwood City, California 94063 (US). DELLAS, Nikki; 200 Penobscot Drive, Red- wood City, California 94063 (US). BASKERVILLE, Don- ald„ S.; 200 Penobscot Drive, Redwood City, California 94063 (US). GOMES, Sandy„ M.; 200 Penobscot Drive, Redwood City, California 94063 (US). ELGART, David; 200 Penobscot Drive, Redwood City, California 94063 (US). VIDUYA, Judy, Victoria Antonio; 200 Penobscot Drive, Redwood City, California94063 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: ENGINEERED LIGASE VARIANTS FIG. 1 Temperature / Activity Profile 90 80 0 70 co 60 -A-SEQ ID NO : 38 0 50 11-WT T4 DNA ligase CU 40 4 , .04 30 7 2 20 15 20 25 30 35 40 Temperature (°C) (57) : The present invention provides engineered ligase polypeptides and compositions thereof, as well as polynucleotides C © encoding the engineered ligase polypeptides. The invention also provides methods for use of the compositions comprising the engineered N • ligase polypeptides for diagnostic and other purposes. [Continued on next page] 0 10 0 00 O 00 WO 2018/208665 Al MIDEDIMOMMIDEFIE1111111111111111111111111101111111111111111111111111 Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))
SG11201909957T2017-05-082018-05-07Engineered ligase variantsSG11201909957TA (en)

Applications Claiming Priority (4)

Application NumberPriority DateFiling DateTitle
US201762503075P2017-05-082017-05-08
US201762540734P2017-08-032017-08-03
US201762587030P2017-11-162017-11-16
PCT/US2018/031376WO2018208665A1 (en)2017-05-082018-05-07Engineered ligase variants

Publications (1)

Publication NumberPublication Date
SG11201909957TAtrue SG11201909957TA (en)2019-11-28

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Family Applications (1)

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SG11201909957TSG11201909957TA (en)2017-05-082018-05-07Engineered ligase variants

Country Status (15)

CountryLink
US (1)US12344872B2 (en)
EP (2)EP3635101B1 (en)
JP (2)JP7401902B2 (en)
KR (1)KR20200003903A (en)
CN (1)CN110914415B (en)
AU (1)AU2018266606B2 (en)
BR (1)BR112019023337A2 (en)
CA (1)CA3062550A1 (en)
DK (1)DK3635101T3 (en)
ES (1)ES2887078T3 (en)
HU (1)HUE055411T2 (en)
MX (1)MX2019013289A (en)
PL (1)PL3635101T3 (en)
SG (1)SG11201909957TA (en)
WO (1)WO2018208665A1 (en)

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Also Published As

Publication numberPublication date
DK3635101T3 (en)2021-09-20
EP3957723A2 (en)2022-02-23
CN110914415B (en)2024-09-17
JP2023166579A (en)2023-11-21
EP3635101A1 (en)2020-04-15
WO2018208665A1 (en)2018-11-15
EP3635101A4 (en)2020-09-30
KR20200003903A (en)2020-01-10
ES2887078T3 (en)2021-12-21
AU2018266606A1 (en)2019-11-14
JP2020518278A (en)2020-06-25
CA3062550A1 (en)2018-11-15
EP3635101B1 (en)2021-08-18
US20180320162A1 (en)2018-11-08
PL3635101T3 (en)2021-12-13
EP3957723A3 (en)2022-04-20
MX2019013289A (en)2020-01-15
AU2018266606B2 (en)2024-09-05
HUE055411T2 (en)2021-11-29
BR112019023337A2 (en)2020-06-16
CN110914415A (en)2020-03-24
US12344872B2 (en)2025-07-01
JP7401902B2 (en)2023-12-20

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