SG11201809437TA - Intracellular delivery of biomolecules to induce tolerance - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111111111111111111111H11111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2017/192785 Al 09 November 2017 (09.11.2017) W I P0 I P C T (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) International Patent Classification: C12N 15/87 (2006.01) C12N C12M 1/00 (2006.01) A61K C12M 1/12 (2006.01) A61K C12M 3/06 (2006.01) International Application Number: International Filing Date: Publication Language: Priority Data: 62/331,368 03 May 2016 (03.05.2016) Applicant: SQZ BIOTECHNOLOGIES [US/US]; 134 Coolidge Avenue, (US). Inventors: GILBERT, Jonathan, nologies Company, 134 Coolidge 02472 (US). WANG, Bu; c/o SQZ pany, 134 Coolidge Avenue, Watertown, 03 May 2017 (03.05.2017) pany, 134 Coolidge Avenue, Watertown, MA 02472 (US). 5/078 (2010.01) SHAREI, Armon, R.; c/o SQZ Biotechnologies Compa- 39/00 (2006.01) ny, 134 Coolidge Avenue, Watertown, MA 02472 (US). 35/18 (2015.01) MOORE, Finola; c/o SQZ Biotechnologies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). (74) Agent: VANEVSKI, Filip et al.; Morrison & Foerster LLP, PCT/US2017/030932 755 Page Mill Road, Palo Alto, CA 94304-1018 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, US MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, COMPANY PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, Watertown, MA 02472 SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. B.; c/o SQZ Biotech- (84) Designated States (unless otherwise indicated, for every Avenue, Watertown, MA kind of regional protection available): ARIPO (BW, GH, Biotechnologies Com- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, MA 02472 (US). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Biotechnologies Compa- TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, MA 02472 (US). EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Biotechnologies Com- MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, = = LOUGHHEAD, Scott; c/o SQZ ny, 134 Coolidge Avenue, Watertown, BERNSTEIN, Howard; c/o SQZ _ cell Title: INTRACELLULAR DELIVERY FIG. 8 5 4 7 a' 4 C:3 gl . A 3 11 M 2 0 i 6 o OF a BIOMOLECULES TO INDUCE Spleen *x-ar* TOLERANCE Lymph Nodes l x;, l< = (54) = — = = __ T g ri 23 = el V'r 15 = n n ice. , Q I— 0 , 0 ,, a eu a'. = 4 Vf. - -4 , _ _ . 4 z •ca a\" b° & Cr CI 4r§' \" g- 4, and/or suppressing an immune response to an antigen wherein the constriction deforms the cell thereby enters the cell. In some embodiments, the anucleate in a tolerogenic environment to induce tolerance and/ _ passing suppress ,.. t z s e ,6 CP ( 46 \" : The present invention provides methods for inducing tolerance a cell suspension containing an anucleate cell through a constriction, a perturbation of the cell such that an antigen and/or tolerogenic factor is delivered to an individual and the antigen is delivered to and processed an immune response to the antigen. 1 ir) GC IN ei (57) 01 by 1-1 ---- causing IN C:0) or ei C [Continued on next page] WO 2017/192785 Al III V TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))