SG11201805493YA - Binding members with altered diversity scaffold domains - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 13 July 2017 (13.07.2017) WIPO I PCT (10) International Publication Number WO 2017/118761 Al 1111111111111101110111111111110101111101110111011111101111111111011111111111110111111 (51) International Patent Classification: C12N 15/10 (2006.01) (21) International Application Number: PCT/EP2017/050337 (22) International Filing Date: 9 January 2017 (09.01.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1600341.0 8 January 2016 (08.01.2016) GB 1621070.0 12 December 2016 (12.12.2016) GB (71) Applicant: IONTAS LTD [GB/GB]; 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). (72) Inventors: KARATT VELLATT, Aneesh; Iontas Ltd, 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). MCCAFFERTY, John; Iontas Ltd, 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). SURADE, Sachin; Iontas Ltd, 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). LUETKENS, Tim; 1163 E 200 S., Salt Lake City, UT 84102 (US). MASTERS, Edward,; Iontas Ltd, 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). DYSON, Michael; Iontas Ltd, 82B High Street, Sawston, Cambridge Cambridgeshire CB22 3HJ (GB). (74) Agents: SUTCLIFFE, Nicholas et al.; Mewburn Ellis LLP, City Tower, 40 Basinghall Street, London Greater London EC2V 5DE (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) W O 20 171187 / 6 1 Al (54) Title: BINDING MEMBERS WITH ALTERED DIVERSITY SCAFFOLD DOMAINS (57) : This invention relates to libraries of binding members that each comprise a fusion protein which contains a donor di - versity scaffold domain, such as a cysteine rich protein,inserted within a recipient diversity scaffold domain,such as an antibody con- stant or variable domain. Libraries and methods of generating libraries are provided, along with screening methods, binding mem- bers and methods of using the binding members.