SG11201704961VA - Suspension culturing of pluripotent stem cells - Google Patents

Suspension culturing of pluripotent stem cells

Info

Publication number: SG11201704961VA
Authority: SG; Singapore
Prior art keywords: stem cells; pluripotent stem; suspension culturing; culturing; suspension
Prior art date: 2014-12-19

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2015-12-09Application filed by Janssen Biotech IncfiledCriticalJanssen Biotech Inc

2017-07-28Publication of SG11201704961VApublicationCriticalpatent/SG11201704961VA/en

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/19—Growth and differentiation factors [GDF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

SG11201704961VA2014-12-192015-12-09Suspension culturing of pluripotent stem cellsSG11201704961VA (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201462094509P	2014-12-19	2014-12-19
PCT/US2015/064713WO2016100035A1 (en)	2014-12-19	2015-12-09	Suspension culturing of pluripotent stem cells

Publications (1)

Publication Number	Publication Date
SG11201704961VAtrue SG11201704961VA (en)	2017-07-28

Family

ID=56127372

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SG11201704961VASG11201704961VA (en)	2014-12-19	2015-12-09	Suspension culturing of pluripotent stem cells

Country Status (16)

Country	Link
US (1)	US20160215268A1 (en)
EP (1)	EP3234109A4 (en)
JP (1)	JP6800854B2 (en)
KR (3)	KR102519502B1 (en)
CN (1)	CN107250349A (en)
AR (1)	AR103080A1 (en)
AU (1)	AU2015363008B2 (en)
BR (1)	BR112017012974A2 (en)
CA (1)	CA2970935A1 (en)
MX (1)	MX2017008176A (en)
PH (1)	PH12017501153A1 (en)
RU (1)	RU2017125728A (en)
SG (1)	SG11201704961VA (en)
TW (1)	TW201636421A (en)
WO (1)	WO2016100035A1 (en)
ZA (1)	ZA201704868B (en)

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CA3012603A1 (en)	2016-01-26	2017-08-03	Centre For Commercialization Of Regenerative Medicine	Method for dissociating cell aggregates
US10391156B2 (en)	2017-07-12	2019-08-27	Viacyte, Inc.	University donor cells and related methods
CN111246864A (en)*	2017-07-21	2020-06-05	森玛治疗公司	Repopulation of stem cell-derived pancreatic beta cells
MX2020002421A (en)*	2017-09-11	2020-07-13	Novo Nordisk As	Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells.
IL305391B2 (en)*	2017-11-15	2024-09-01	Vertex Pharma	Islet cell manufacturing compositions and methods of use
AU2019320072A1 (en)	2018-08-10	2021-02-25	Vertex Pharmaceuticals Incorporated	Stem cell derived islet differentiation
US20200080107A1 (en)	2018-09-07	2020-03-12	Crispr Therapeutics Ag	Universal donor cells
TWI862513B (en)*	2018-09-19	2024-11-21	日商千紙鶴治療公司	Cell group containing insulin producing cells, medicines containing the producing cells, and uses and manufacturing methods thereof
AU2020282355B2 (en)	2019-05-31	2023-11-02	Viacyte, Inc.	A biocompatible membrane composite
AU2020283150B2 (en)	2019-05-31	2023-08-17	Viacyte, Inc.	Cell encapsulation devices with controlled oxygen diffusion distances
AU2020284245B2 (en)	2019-05-31	2023-10-05	Viacyte, Inc.	A biocompatible membrane composite
EP3975925A1 (en)	2019-05-31	2022-04-06	W.L. Gore & Associates, Inc.	A biocompatible membrane composite
EP4025224A1 (en)	2019-09-05	2022-07-13	CRISPR Therapeutics AG	Universal donor cells
PH12022550543A1 (en)	2019-09-05	2023-03-20	Crispr Therapeutics Ag	Universal donor cells
EP4271796A1 (en)	2020-12-31	2023-11-08	CRISPR Therapeutics AG	Universal donor cells

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US5843780A (en)	1995-01-20	1998-12-01	Wisconsin Alumni Research Foundation	Primate embryonic stem cells
KR101089591B1 (en)	2001-12-07	2011-12-05	제론 코포레이션	Islet cells from human embryonic stem cells
CA2530732C (en)	2003-06-27	2015-03-31	Ethicon, Incorporated	Regeneration and repair of neural tissue using postpartum-derived cells
CN103898047B (en)	2003-12-23	2020-03-03	维亚希特公司	Definitive endoderm
US20050266554A1 (en)	2004-04-27	2005-12-01	D Amour Kevin A	PDX1 expressing endoderm
CA2560581C (en)	2004-03-23	2013-06-11	Toshihiro Akaike	Pluripotent stem cell growing method
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US7695965B2 (en)	2006-03-02	2010-04-13	Cythera, Inc.	Methods of producing pancreatic hormones
WO2008013664A2 (en)	2006-07-26	2008-01-31	Cythera, Inc.	Methods of producing pancreatic hormones
ES2874223T3 (en)	2006-08-02	2021-11-04	Technion Res & Dev Foundation	Embryonic stem cell expansion methods in suspension culture
JP5991796B2 (en)	2007-06-29	2016-09-14	セルラーダイナミクスインターナショナル，インコーポレイテッド	Automated method and apparatus for embryonic stem cell culture
JP5734183B2 (en)	2008-06-30	2015-06-17	ヤンセンバイオテツク，インコーポレーテツド	Differentiation of pluripotent stem cells
WO2010022395A2 (en)*	2008-08-22	2010-02-25	President And Fellows Of Harvard College	Methods of reprogramming cells
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2015
- 2015-12-09CACA2970935Apatent/CA2970935A1/enactivePending
- 2015-12-09AUAU2015363008Apatent/AU2015363008B2/enactiveActive
- 2015-12-09RURU2017125728Apatent/RU2017125728A/ennot_activeApplication Discontinuation
- 2015-12-09WOPCT/US2015/064713patent/WO2016100035A1/enactiveApplication Filing
- 2015-12-09SGSG11201704961VApatent/SG11201704961VA/enunknown
- 2015-12-09USUS14/963,730patent/US20160215268A1/ennot_activeAbandoned
- 2015-12-09KRKR1020217022958Apatent/KR102519502B1/enactiveActive
- 2015-12-09EPEP15870718.2Apatent/EP3234109A4/enactivePending
- 2015-12-09KRKR1020237011455Apatent/KR102700499B1/enactiveActive
- 2015-12-09KRKR1020177019548Apatent/KR102281752B1/enactiveActive
- 2015-12-09JPJP2017532641Apatent/JP6800854B2/enactiveActive
- 2015-12-09BRBR112017012974Apatent/BR112017012974A2/ennot_activeApplication Discontinuation
- 2015-12-09CNCN201580076646.0Apatent/CN107250349A/enactivePending
- 2015-12-09MXMX2017008176Apatent/MX2017008176A/enunknown
- 2015-12-17ARARP150104154Apatent/AR103080A1/enunknown
- 2015-12-17TWTW104142402Apatent/TW201636421A/enunknown
2017
- 2017-06-19PHPH12017501153Apatent/PH12017501153A1/enunknown
- 2017-07-18ZAZA2017/04868Apatent/ZA201704868B/enunknown

Also Published As

Publication number	Publication date
KR20170087520A (en)	2017-07-28
CN107250349A (en)	2017-10-13
RU2017125728A3 (en)	2019-06-03
KR102519502B1 (en)	2023-04-06
CA2970935A1 (en)	2016-06-23
MX2017008176A (en)	2018-02-09
AU2015363008B2 (en)	2021-12-09
AU2015363008A1 (en)	2017-06-29
KR102700499B1 (en)	2024-08-28
PH12017501153A1 (en)	2017-11-27
KR102281752B1 (en)	2021-07-23
US20160215268A1 (en)	2016-07-28
JP2017537649A (en)	2017-12-21
EP3234109A1 (en)	2017-10-25
AR103080A1 (en)	2017-04-12
KR20230050478A (en)	2023-04-14
BR112017012974A2 (en)	2018-01-09
WO2016100035A1 (en)	2016-06-23
RU2017125728A (en)	2019-01-22
JP6800854B2 (en)	2020-12-16
EP3234109A4 (en)	2018-06-27
KR20210094141A (en)	2021-07-28
ZA201704868B (en)	2019-02-27
TW201636421A (en)	2016-10-16

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ZA201704868B (en)	2019-02-27	Suspension culturing of pluripotent stem cells
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SG11201609269TA (en)	2016-12-29	Method of culturing cells

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SG11201704961VA - Suspension culturing of pluripotent stem cells - Google Patents

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Applications Claiming Priority (2)

Publications (1)

Family

ID=56127372

Family Applications (1)

Country Status (16)

Families Citing this family (15)

Family Cites Families (22)

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