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NZ240079A - Method for the detection of a nucleic acid or part thereof - Google Patents

Method for the detection of a nucleic acid or part thereof

Info

Publication number
NZ240079A
NZ240079ANZ240079ANZ24007991ANZ240079ANZ 240079 ANZ240079 ANZ 240079ANZ 240079 ANZ240079 ANZ 240079ANZ 24007991 ANZ24007991 ANZ 24007991ANZ 240079 ANZ240079 ANZ 240079A
Authority
NZ
New Zealand
Prior art keywords
nucleic acid
sample
solid phase
reacted
detection
Prior art date
Application number
NZ240079A
Inventor
Christoph Kessler
Ruediger Rueger
Cornelia Kruse-Mueller
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4038804Aexternal-prioritypatent/DE4038804A1/en
Priority claimed from DE4041608Aexternal-prioritypatent/DE4041608A1/en
Application filed by Boehringer Mannheim GmbhfiledCriticalBoehringer Mannheim Gmbh
Publication of NZ240079ApublicationCriticalpatent/NZ240079A/en

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Abstract

A process is disclosed for specifically detecting nucleic acids in a sample. The sample is reacted with one or several labeled nucleotide triphosphates and one or several enzymes which catalyze the production of a labeled nucleic acid B containing said nucleotide. Then the sample is subjected to a non thermal denaturation and reacted with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group. The possibly formed nucleic acid hybrid D is contacted with a solid phase which recognizes and binds the immobilizable group, the liquid phase is removed from the solid phase and the labeling of the solid phase is considered as a measure of the presence of nucleic acids.
NZ240079A1990-10-091991-10-03Method for the detection of a nucleic acid or part thereofNZ240079A (en)

Applications Claiming Priority (3)

Application NumberPriority DateFiling DateTitle
DE40320241990-10-09
DE4038804ADE4038804A1 (en)1990-10-091990-12-05 METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID
DE4041608ADE4041608A1 (en)1990-12-221990-12-22Sensitive and selective nucleic acid detection

Publications (1)

Publication NumberPublication Date
NZ240079Atrue NZ240079A (en)1993-07-27

Family

ID=27201764

Family Applications (1)

Application NumberTitlePriority DateFiling Date
NZ240079ANZ240079A (en)1990-10-091991-10-03Method for the detection of a nucleic acid or part thereof

Country Status (15)

CountryLink
EP (1)EP0552203B1 (en)
JP (1)JP2673162B2 (en)
AT (1)ATE122104T1 (en)
AU (1)AU655781B2 (en)
CA (1)CA2093647C (en)
DE (1)DE59105402D1 (en)
DK (1)DK0552203T3 (en)
ES (1)ES2073771T3 (en)
FI (1)FI103808B1 (en)
IE (1)IE68525B1 (en)
IL (1)IL99663A (en)
NO (1)NO305995B1 (en)
NZ (1)NZ240079A (en)
PT (1)PT99177B (en)
WO (1)WO1992006216A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
DE4344742A1 (en)*1993-06-091994-12-15Boehringer Mannheim Gmbh Method for the immobilization of nucleic acids
DK0702728T3 (en)*1993-06-091998-06-02Gamera Bioscience Corp Magnetic cycle reaction
RU2146707C1 (en)*1996-04-112000-03-20Куликова Валентина ФилипповнаMethod of detection of analyzed sequence of nucleic acid
ES2252011T3 (en)*1999-05-072006-05-16Roche Diagnostics Gmbh HIGH DENSITY MARKING OF DNA WITH MODIFIED NUCLEOTIDES OR WHICH CARRY AN EMPLOYED CHROMOPHORE AND DNA-POLYMERASE.
CA2506654C (en)*2002-11-072014-02-25Yoichi MatsubaraMethod of detecting gene mutation

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
ZA862335B (en)*1985-03-281987-11-25Cetus CorpProcess for amplifying nucleic acid sequences
AU622104B2 (en)*1987-03-111992-04-02Sangtec Molecular Diagnostics AbMethod of assaying of nucleic acids, a reagent combination and kit therefore
JP2650159B2 (en)*1988-02-241997-09-03アクゾ・ノベル・エヌ・ベー Nucleic acid amplification method
CA1340807C (en)*1988-02-241999-11-02Lawrence T. MalekNucleic acid amplification process
AT390080B (en)*1988-07-291990-03-12Lion Thomas Dr FAST BLOT METHOD FOR DETECTING VIRAL SEQUENCES AND AMPLIFIED GENE SEQUENCES IN HUMAN CELLS
CA2004326A1 (en)*1988-12-231990-06-23Nanibushan DattaguptaAssay of sequences using amplified genes
KR920700360A (en)*1989-03-221992-02-19하리크 프리드리히 Sliding bearing
CA2012982A1 (en)*1989-03-271990-09-27Frank W. HobbsProcess for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid
US5043272A (en)*1989-04-271991-08-27Life Technologies, IncorporatedAmplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5232829A (en)*1989-09-291993-08-03Hoffmann-La Roche Inc.Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes

Also Published As

Publication numberPublication date
CA2093647A1 (en)1992-04-10
FI103808B (en)1999-09-30
WO1992006216A1 (en)1992-04-16
JPH06501157A (en)1994-02-10
NO305995B1 (en)1999-08-30
NO931309L (en)1993-04-06
PT99177B (en)1999-04-30
CA2093647C (en)2000-09-19
IE68525B1 (en)1996-06-26
DK0552203T3 (en)1995-10-02
EP0552203A1 (en)1993-07-28
EP0552203B1 (en)1995-05-03
FI931586L (en)1993-04-07
FI103808B1 (en)1999-09-30
ATE122104T1 (en)1995-05-15
DE59105402D1 (en)1995-06-08
IE913552A1 (en)1992-04-22
FI931586A0 (en)1993-04-07
AU655781B2 (en)1995-01-12
NO931309D0 (en)1993-04-06
PT99177A (en)1992-09-30
JP2673162B2 (en)1997-11-05
IL99663A (en)1995-12-31
AU8627591A (en)1992-04-28
IL99663A0 (en)1992-08-18
ES2073771T3 (en)1995-08-16

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